Quadruple labeling of brain-stem neurons: a multiple retrograde fluorescent tracer study of axonal collateralization

Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction t...

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Veröffentlicht in:Journal of neuroscience methods 1992-10, Vol.45 (1), p.15-22
Hauptverfasser: Akintunde, Akinsegun, Buxton, Donald F.
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Sprache:eng
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