Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides
Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryot...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2010-05, Vol.58 (9), p.5380-5386 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Islam, Shah Md. Asraful Math, Renukaradhya K Cho, Kye Man Lim, Woo Jin Hong, Su Young Kim, Jong Min Yun, Myoung Geun Cho, Ji Joong Yun, Han Dae |
| description | Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 °C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion. |
| doi_str_mv | 10.1021/jf903878e |
| format | Article |
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Asraful ; Math, Renukaradhya K ; Cho, Kye Man ; Lim, Woo Jin ; Hong, Su Young ; Kim, Jong Min ; Yun, Myoung Geun ; Cho, Ji Joong ; Yun, Han Dae</creator><creatorcontrib>Islam, Shah Md. Asraful ; Math, Renukaradhya K ; Cho, Kye Man ; Lim, Woo Jin ; Hong, Su Young ; Kim, Jong Min ; Yun, Myoung Geun ; Cho, Ji Joong ; Yun, Han Dae</creatorcontrib><description>Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 °C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf903878e</identifier><identifier>PMID: 20405842</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Aryldialkylphosphatase - metabolism ; Base Sequence ; biodegradation ; Biological and medical sciences ; Chemical Aspects of Biotechnology/Molecular Biology ; chlorpyrifos ; DNA Primers ; enzyme activity ; food contamination ; Food industries ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; General aspects ; Hygiene and safety ; kimchi ; lactic acid bacteria ; Lactobacillus brevis ; Lactobacillus brevis - enzymology ; Lactobacillus brevis WCP902 ; model food systems ; Organophosphorus Compounds - metabolism ; organophosphorus hydrolase (OpdB) ; organophosphorus pesticides ; pesticide residues ; Pesticides - metabolism</subject><ispartof>Journal of agricultural and food chemistry, 2010-05, Vol.58 (9), p.5380-5386</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a434t-d2cd7dd64cdc9d51508786db7be6d8f06957c543f0c917e23e635e84f8e0d5a13</citedby><cites>FETCH-LOGICAL-a434t-d2cd7dd64cdc9d51508786db7be6d8f06957c543f0c917e23e635e84f8e0d5a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf903878e$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf903878e$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27055,27903,27904,56716,56766</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22764410$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20405842$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Islam, Shah Md. Asraful</creatorcontrib><creatorcontrib>Math, Renukaradhya K</creatorcontrib><creatorcontrib>Cho, Kye Man</creatorcontrib><creatorcontrib>Lim, Woo Jin</creatorcontrib><creatorcontrib>Hong, Su Young</creatorcontrib><creatorcontrib>Kim, Jong Min</creatorcontrib><creatorcontrib>Yun, Myoung Geun</creatorcontrib><creatorcontrib>Cho, Ji Joong</creatorcontrib><creatorcontrib>Yun, Han Dae</creatorcontrib><title>Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 °C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion.</description><subject>Aryldialkylphosphatase - metabolism</subject><subject>Base Sequence</subject><subject>biodegradation</subject><subject>Biological and medical sciences</subject><subject>Chemical Aspects of Biotechnology/Molecular Biology</subject><subject>chlorpyrifos</subject><subject>DNA Primers</subject><subject>enzyme activity</subject><subject>food contamination</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects</subject><subject>Hygiene and safety</subject><subject>kimchi</subject><subject>lactic acid bacteria</subject><subject>Lactobacillus brevis</subject><subject>Lactobacillus brevis - enzymology</subject><subject>Lactobacillus brevis WCP902</subject><subject>model food systems</subject><subject>Organophosphorus Compounds - metabolism</subject><subject>organophosphorus hydrolase (OpdB)</subject><subject>organophosphorus pesticides</subject><subject>pesticide residues</subject><subject>Pesticides - metabolism</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0MtuEzEUBmALgWhaWPAC4A2CLgaObzOeZQmXVkRKJVqxHHns49TRTJzaGaSqL49RQruAheWFP_3n-CfkFYMPDDj7uPYtCN1ofEJmTHGoFGP6KZlBeay0qtkROc55DQBaNfCcHHGQoLTkM3K_TCuzidubmMtJU6bndy7FwWSk75db9-mURk8Xxu5ib2wYhiL6hL9Cpj_nly1w6lMc6fcw2ptALzI96wekV5F-xlUyDuk_-ZeYd8EGh_kFeebNkPHl4T4h11-_XM3Pq8Xy28X8bFEZKeSucty6xrlaWmdbp5iC8tXa9U2PtdMe6lY1VknhwbasQS6wFgq19BrBKcPECXm3z92meDuV8d0YssVhMBuMU-4aIVrZQFsXebqXNsWcE_pum8Jo0l3HoPtTdfdQdbGvD6lTP6J7kH-7LeDtAZhszeCT2diQHx1vaikZFPdm77yJnVmlYq5_cGACWIlptXhMMjZ36zilTanrPyv9Bsuom2s</recordid><startdate>20100512</startdate><enddate>20100512</enddate><creator>Islam, Shah Md. Asraful</creator><creator>Math, Renukaradhya K</creator><creator>Cho, Kye Man</creator><creator>Lim, Woo Jin</creator><creator>Hong, Su Young</creator><creator>Kim, Jong Min</creator><creator>Yun, Myoung Geun</creator><creator>Cho, Ji Joong</creator><creator>Yun, Han Dae</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100512</creationdate><title>Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides</title><author>Islam, Shah Md. Asraful ; Math, Renukaradhya K ; Cho, Kye Man ; Lim, Woo Jin ; Hong, Su Young ; Kim, Jong Min ; Yun, Myoung Geun ; Cho, Ji Joong ; Yun, Han Dae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a434t-d2cd7dd64cdc9d51508786db7be6d8f06957c543f0c917e23e635e84f8e0d5a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aryldialkylphosphatase - metabolism</topic><topic>Base Sequence</topic><topic>biodegradation</topic><topic>Biological and medical sciences</topic><topic>Chemical Aspects of Biotechnology/Molecular Biology</topic><topic>chlorpyrifos</topic><topic>DNA Primers</topic><topic>enzyme activity</topic><topic>food contamination</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>Hygiene and safety</topic><topic>kimchi</topic><topic>lactic acid bacteria</topic><topic>Lactobacillus brevis</topic><topic>Lactobacillus brevis - enzymology</topic><topic>Lactobacillus brevis WCP902</topic><topic>model food systems</topic><topic>Organophosphorus Compounds - metabolism</topic><topic>organophosphorus hydrolase (OpdB)</topic><topic>organophosphorus pesticides</topic><topic>pesticide residues</topic><topic>Pesticides - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Islam, Shah Md. Asraful</creatorcontrib><creatorcontrib>Math, Renukaradhya K</creatorcontrib><creatorcontrib>Cho, Kye Man</creatorcontrib><creatorcontrib>Lim, Woo Jin</creatorcontrib><creatorcontrib>Hong, Su Young</creatorcontrib><creatorcontrib>Kim, Jong Min</creatorcontrib><creatorcontrib>Yun, Myoung Geun</creatorcontrib><creatorcontrib>Cho, Ji Joong</creatorcontrib><creatorcontrib>Yun, Han Dae</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Islam, Shah Md. Asraful</au><au>Math, Renukaradhya K</au><au>Cho, Kye Man</au><au>Lim, Woo Jin</au><au>Hong, Su Young</au><au>Kim, Jong Min</au><au>Yun, Myoung Geun</au><au>Cho, Ji Joong</au><au>Yun, Han Dae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2010-05-12</date><risdate>2010</risdate><volume>58</volume><issue>9</issue><spage>5380</spage><epage>5386</epage><pages>5380-5386</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>Lactobacillus brevis WCP902 that is capable of biodegrading chlorpyrifos was isolated from kimchi. The opdB gene cloned from this strain revealed 825 bp, encoding 274 aa, and an enzyme molecular weight of about 27 kDa. OpdB contains the same Gly-X-Ser-X-Gly motif found in most bacterial and eukaryotic esterase, lipase, and serine hydrolases, yet it is a novel member of the GDSVG family of esterolytic enzymes. Its conserved serine residue, Ser82, is significantly involved with enzyme activity that may have application for removing some pesticides. Optimum organophosphorus hydrolase (OpdB) activity appeared at pH 6.0 and 35 °C and during degradation of chlorpyrifos, coumaphos, diazinon, methylparathion, and parathion.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20405842</pmid><doi>10.1021/jf903878e</doi><tpages>7</tpages></addata></record> |
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| subjects | Aryldialkylphosphatase - metabolism Base Sequence biodegradation Biological and medical sciences Chemical Aspects of Biotechnology/Molecular Biology chlorpyrifos DNA Primers enzyme activity food contamination Food industries Food microbiology Fundamental and applied biological sciences. Psychology General aspects Hygiene and safety kimchi lactic acid bacteria Lactobacillus brevis Lactobacillus brevis - enzymology Lactobacillus brevis WCP902 model food systems Organophosphorus Compounds - metabolism organophosphorus hydrolase (OpdB) organophosphorus pesticides pesticide residues Pesticides - metabolism |
| title | Organophosphorus Hydrolase (OpdB) of Lactobacillus brevis WCP902 from Kimchi Is Able To Degrade Organophosphorus Pesticides |
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