Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing
Abstract The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primar...
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| Veröffentlicht in: | Biomaterials 2009-10, Vol.30 (29), p.5427-5432 |
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| creator | Elloumi Hannachi, Imen Itoga, Kazuyoshi Kumashiro, Yoshikazu Kobayashi, Jun Yamato, Masayuki Okano, Teruo |
| description | Abstract The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture. |
| doi_str_mv | 10.1016/j.biomaterials.2009.06.033 |
| format | Article |
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To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2009.06.033</identifier><identifier>PMID: 19608271</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; Animals ; Biocompatible Materials - chemistry ; Cattle ; Cell sheet ; Cells, Cultured ; Co-culture ; Coculture Techniques - methods ; Dentistry ; Endothelial Cells - cytology ; Endothelial Cells - physiology ; Hepatocytes - cytology ; Hepatocytes - physiology ; Maskless photolithography ; Materials Testing ; Microcontact printing ; Micropatterning ; Rats ; Surface Properties ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2009-10, Vol.30 (29), p.5427-5432</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-95359db986abaf4b436da5b6fb3f8ef3f6bb79871f08cc3ed78fb74b7dc62e753</citedby><cites>FETCH-LOGICAL-c496t-95359db986abaf4b436da5b6fb3f8ef3f6bb79871f08cc3ed78fb74b7dc62e753</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2009.06.033$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19608271$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Elloumi Hannachi, Imen</creatorcontrib><creatorcontrib>Itoga, Kazuyoshi</creatorcontrib><creatorcontrib>Kumashiro, Yoshikazu</creatorcontrib><creatorcontrib>Kobayashi, Jun</creatorcontrib><creatorcontrib>Yamato, Masayuki</creatorcontrib><creatorcontrib>Okano, Teruo</creatorcontrib><title>Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture.</description><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Cattle</subject><subject>Cell sheet</subject><subject>Cells, Cultured</subject><subject>Co-culture</subject><subject>Coculture Techniques - methods</subject><subject>Dentistry</subject><subject>Endothelial Cells - cytology</subject><subject>Endothelial Cells - physiology</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - physiology</subject><subject>Maskless photolithography</subject><subject>Materials Testing</subject><subject>Microcontact printing</subject><subject>Micropatterning</subject><subject>Rats</subject><subject>Surface Properties</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkkGLFDEQhYMo7uzqX5DGg566TTrppONBkHVXhQUPKniRkKQrbsaeZDZJK_vvTTsDigf1FALfq1dVrxB6THBHMOHPtp3xcacLJK_n3PUYyw7zDlN6B23IKMZ2kHi4izaYsL6VnPQn6DTnLa5_zPr76IRIjsdekA36fKlN8lYXH0MTXVOSDtlB0maGZudtintdqlOAqbWxtctclgRTY2Gem3wNUHLz3ZfrA2tjKNqWZp98KD58eYDuudoiPDy-Z-jj5cWH8zft1bvXb89fXrWWSV5aOdBBTkaOXBvtmGGUT3ow3BnqRnDUcWOEHAVxeLSWwiRGZwQzYrK8BzHQM_T0UHef4s0Cuaidz2uLOkBcshKUSjYQJiv55K8kZYKNA6H_BHsshGQ_vZ8fwDp_zgmcqtPvdLpVBKs1L7VVv-el1rwU5qrmVcWPji6L2cH0S3oMqAKvDgDU9X3zkFS2HoKFySewRU3R_5_Piz_K2NmHmvv8FW4hb-OSwqohKvcKq_fr5ayHgyXGnPJP9Adv9sU-</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Elloumi Hannachi, Imen</creator><creator>Itoga, Kazuyoshi</creator><creator>Kumashiro, Yoshikazu</creator><creator>Kobayashi, Jun</creator><creator>Yamato, Masayuki</creator><creator>Okano, Teruo</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20091001</creationdate><title>Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing</title><author>Elloumi Hannachi, Imen ; Itoga, Kazuyoshi ; Kumashiro, Yoshikazu ; Kobayashi, Jun ; Yamato, Masayuki ; Okano, Teruo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-95359db986abaf4b436da5b6fb3f8ef3f6bb79871f08cc3ed78fb74b7dc62e753</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Advanced Basic Science</topic><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Cattle</topic><topic>Cell sheet</topic><topic>Cells, Cultured</topic><topic>Co-culture</topic><topic>Coculture Techniques - methods</topic><topic>Dentistry</topic><topic>Endothelial Cells - cytology</topic><topic>Endothelial Cells - physiology</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - physiology</topic><topic>Maskless photolithography</topic><topic>Materials Testing</topic><topic>Microcontact printing</topic><topic>Micropatterning</topic><topic>Rats</topic><topic>Surface Properties</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Elloumi Hannachi, Imen</creatorcontrib><creatorcontrib>Itoga, Kazuyoshi</creatorcontrib><creatorcontrib>Kumashiro, Yoshikazu</creatorcontrib><creatorcontrib>Kobayashi, Jun</creatorcontrib><creatorcontrib>Yamato, Masayuki</creatorcontrib><creatorcontrib>Okano, Teruo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Elloumi Hannachi, Imen</au><au>Itoga, Kazuyoshi</au><au>Kumashiro, Yoshikazu</au><au>Kobayashi, Jun</au><au>Yamato, Masayuki</au><au>Okano, Teruo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>30</volume><issue>29</issue><spage>5427</spage><epage>5432</epage><pages>5427-5432</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract The purpose of the present study is to develop a novel method for the fabrication of transferable micropatterned cell sheets for tissue engineering. To achieve this development, microcontact printing of fibronectin on commercially available temperature-responsive dishes was employed. Primary rat hepatocytes were seeded on the dish surfaces printed with fibronectin. Under serum-free conditions, hepatocytes were attached onto fibronectin domains selectively. Then, a second cell type of endothelial cells was seeded in the presence of serum. Double fluorescent staining revealed that endothelial cells successfully adhered to the intervals of hepatocyte domains. Finally, all the cells were harvested as a single contiguous micropatterned cell sheet upon temperature-reduction. With a cell sheet manipulator having a gelatin layer for the support of harvested cell sheets, harvested micropatterned cell sheets were transferred to new dish surfaces. This technique would be useful for the fabrication of thick tissue constructs having a complex microarchitecture.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>19608271</pmid><doi>10.1016/j.biomaterials.2009.06.033</doi><tpages>6</tpages></addata></record> |
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| subjects | Advanced Basic Science Animals Biocompatible Materials - chemistry Cattle Cell sheet Cells, Cultured Co-culture Coculture Techniques - methods Dentistry Endothelial Cells - cytology Endothelial Cells - physiology Hepatocytes - cytology Hepatocytes - physiology Maskless photolithography Materials Testing Microcontact printing Micropatterning Rats Surface Properties Tissue engineering Tissue Engineering - methods |
| title | Fabrication of transferable micropatterned-co-cultured cell sheets with microcontact printing |
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