Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia
Objective To investigate the effects of Kangquan Recipe (康泉方, KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats. Methods Seventy-two SD rats were randomly divided into six groups: the normal group, the model group, the finasteride group,...
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| Veröffentlicht in: | Chinese journal of integrative medicine 2009-08, Vol.15 (4), p.289-292 |
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| creator | Huang, Yuan-peng Du, Jian Hong, Zhen-feng Chen, Zhi-qing Wu, Jin-fa Zhao, Jin-yan |
| description | Objective
To investigate the effects of Kangquan Recipe (康泉方, KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats.
Methods
Seventy-two SD rats were randomly divided into six groups: the normal group, the model group, the finasteride group, and the low-, middle-, and high-dose KQR groups, 12 in each group. Except those in the normal group, the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline, finasteride, and low-, middle-, and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E
2
) were determined by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression ) of proliferating cell nuclear antigen (PCNA) in prostate tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) after administration.
Results
Compared with the model group, the prostate weight, the plasma T, and the mRNA expression of PCNA were significantly lower, and the plasma E
2
and the ratio of E
2
/T were higher in the three KQR groups (
P |
| doi_str_mv | 10.1007/s11655-009-0289-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733943018</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733943018</sourcerecordid><originalsourceid>FETCH-LOGICAL-c343t-e1856e5fbad0226a989f0652ffce814b674df83bdb6bcbd2e16606a5d0361d663</originalsourceid><addsrcrecordid>eNp9kMFu1DAQhi0EasvSB-BS-QYcAuM4dpxjtSqlolKlCs6WE493XWWd1E7U9hF4AV6kBwTv0z4HXu1K3DjNL803v_75CXnL4CMDqD8lxqQQBUBTQKmagr8gR6xpeAEVlC-zlnWZNROH5HVKNwCiliAOyCFrpFKcqSNyd-YcdlOig6NfTVjdzibQa-z8iPT9059fz48_nn_-_kCHQBPe0zRhHLxN1ARLO-x7Osah9w6jmXxmfKBZJXrnpzVtMfhV2BJpyuuOrh9GjGNvkjdvyCtn-oTH-7kg3z-ffVt-KS6vzi-Wp5dFxys-FciUkChcayyUpTSNahxIUTrXoWJVK-vKOsVb28q2a22JTEqQRljgklkp-YK82_nmFLczpklvfNoGNwGHOema86biwFQm2Y7sct4U0ekx-o2JD5qB3tatd3XrXLfe1q15vjnZu8_tBu2_i32_GSh3QMqrsMKob4Y5hvzxf1z_ArAzj6A</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733943018</pqid></control><display><type>article</type><title>Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia</title><source>MEDLINE</source><source>SpringerLink Journals</source><source>Alma/SFX Local Collection</source><creator>Huang, Yuan-peng ; Du, Jian ; Hong, Zhen-feng ; Chen, Zhi-qing ; Wu, Jin-fa ; Zhao, Jin-yan</creator><creatorcontrib>Huang, Yuan-peng ; Du, Jian ; Hong, Zhen-feng ; Chen, Zhi-qing ; Wu, Jin-fa ; Zhao, Jin-yan</creatorcontrib><description>Objective
To investigate the effects of Kangquan Recipe (康泉方, KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats.
Methods
Seventy-two SD rats were randomly divided into six groups: the normal group, the model group, the finasteride group, and the low-, middle-, and high-dose KQR groups, 12 in each group. Except those in the normal group, the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline, finasteride, and low-, middle-, and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E
2
) were determined by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression ) of proliferating cell nuclear antigen (PCNA) in prostate tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) after administration.
Results
Compared with the model group, the prostate weight, the plasma T, and the mRNA expression of PCNA were significantly lower, and the plasma E
2
and the ratio of E
2
/T were higher in the three KQR groups (
P
<0.05 or
P
<0.01). There was no significant difference in the prostate weight, plasma T and E
2
, and ratio of E
2
/T among the finasteride group and the three KQR groups (
P
>0.05). The mRNA expressions of PCNA were significantly higher in the middle- and low-dose of KQR groups than those in the finasteride group (
P
<0.05).
Conclusion
KQR shows multitarget effects on experimental BPH rats, and the mechanism might be related with regulating the balance of plasma T and E
2
and decreasing the PCNAmRNA expression in prostate tissue to restrain cell proliferation in a dose-dependent manner.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><identifier>DOI: 10.1007/s11655-009-0289-3</identifier><identifier>PMID: 19688318</identifier><language>eng</language><publisher>Heidelberg: Chinese Association of Traditional and Western Medicine</publisher><subject>Animals ; Body Weight - drug effects ; Cell Proliferation - drug effects ; Cookbooks as Topic ; Drug Evaluation, Preclinical ; Drugs, Chinese Herbal - pharmacology ; Drugs, Chinese Herbal - therapeutic use ; Experimental Research ; Gonadal Steroid Hormones - blood ; Gonadal Steroid Hormones - metabolism ; Male ; Medicine ; Medicine & Public Health ; Medicine, Chinese Traditional - methods ; Organ Size - drug effects ; Proliferating Cell Nuclear Antigen - genetics ; Proliferating Cell Nuclear Antigen - metabolism ; Prostate - drug effects ; Prostate - pathology ; Prostatic Hyperplasia - blood ; Prostatic Hyperplasia - drug therapy ; Prostatic Hyperplasia - metabolism ; Prostatic Hyperplasia - pathology ; Rats ; Rats, Sprague-Dawley</subject><ispartof>Chinese journal of integrative medicine, 2009-08, Vol.15 (4), p.289-292</ispartof><rights>Chinese Association of the Integration of Traditional and Western Medicine and Springer-Verlag GmbH 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-e1856e5fbad0226a989f0652ffce814b674df83bdb6bcbd2e16606a5d0361d663</citedby><cites>FETCH-LOGICAL-c343t-e1856e5fbad0226a989f0652ffce814b674df83bdb6bcbd2e16606a5d0361d663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11655-009-0289-3$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11655-009-0289-3$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19688318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Huang, Yuan-peng</creatorcontrib><creatorcontrib>Du, Jian</creatorcontrib><creatorcontrib>Hong, Zhen-feng</creatorcontrib><creatorcontrib>Chen, Zhi-qing</creatorcontrib><creatorcontrib>Wu, Jin-fa</creatorcontrib><creatorcontrib>Zhao, Jin-yan</creatorcontrib><title>Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia</title><title>Chinese journal of integrative medicine</title><addtitle>Chin. J. Integr. Med</addtitle><addtitle>Chin J Integr Med</addtitle><description>Objective
To investigate the effects of Kangquan Recipe (康泉方, KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats.
Methods
Seventy-two SD rats were randomly divided into six groups: the normal group, the model group, the finasteride group, and the low-, middle-, and high-dose KQR groups, 12 in each group. Except those in the normal group, the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline, finasteride, and low-, middle-, and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E
2
) were determined by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression ) of proliferating cell nuclear antigen (PCNA) in prostate tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) after administration.
Results
Compared with the model group, the prostate weight, the plasma T, and the mRNA expression of PCNA were significantly lower, and the plasma E
2
and the ratio of E
2
/T were higher in the three KQR groups (
P
<0.05 or
P
<0.01). There was no significant difference in the prostate weight, plasma T and E
2
, and ratio of E
2
/T among the finasteride group and the three KQR groups (
P
>0.05). The mRNA expressions of PCNA were significantly higher in the middle- and low-dose of KQR groups than those in the finasteride group (
P
<0.05).
Conclusion
KQR shows multitarget effects on experimental BPH rats, and the mechanism might be related with regulating the balance of plasma T and E
2
and decreasing the PCNAmRNA expression in prostate tissue to restrain cell proliferation in a dose-dependent manner.</description><subject>Animals</subject><subject>Body Weight - drug effects</subject><subject>Cell Proliferation - drug effects</subject><subject>Cookbooks as Topic</subject><subject>Drug Evaluation, Preclinical</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Drugs, Chinese Herbal - therapeutic use</subject><subject>Experimental Research</subject><subject>Gonadal Steroid Hormones - blood</subject><subject>Gonadal Steroid Hormones - metabolism</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Medicine, Chinese Traditional - methods</subject><subject>Organ Size - drug effects</subject><subject>Proliferating Cell Nuclear Antigen - genetics</subject><subject>Proliferating Cell Nuclear Antigen - metabolism</subject><subject>Prostate - drug effects</subject><subject>Prostate - pathology</subject><subject>Prostatic Hyperplasia - blood</subject><subject>Prostatic Hyperplasia - drug therapy</subject><subject>Prostatic Hyperplasia - metabolism</subject><subject>Prostatic Hyperplasia - pathology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFu1DAQhi0EasvSB-BS-QYcAuM4dpxjtSqlolKlCs6WE493XWWd1E7U9hF4AV6kBwTv0z4HXu1K3DjNL803v_75CXnL4CMDqD8lxqQQBUBTQKmagr8gR6xpeAEVlC-zlnWZNROH5HVKNwCiliAOyCFrpFKcqSNyd-YcdlOig6NfTVjdzibQa-z8iPT9059fz48_nn_-_kCHQBPe0zRhHLxN1ARLO-x7Osah9w6jmXxmfKBZJXrnpzVtMfhV2BJpyuuOrh9GjGNvkjdvyCtn-oTH-7kg3z-ffVt-KS6vzi-Wp5dFxys-FciUkChcayyUpTSNahxIUTrXoWJVK-vKOsVb28q2a22JTEqQRljgklkp-YK82_nmFLczpklvfNoGNwGHOema86biwFQm2Y7sct4U0ekx-o2JD5qB3tatd3XrXLfe1q15vjnZu8_tBu2_i32_GSh3QMqrsMKob4Y5hvzxf1z_ArAzj6A</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Huang, Yuan-peng</creator><creator>Du, Jian</creator><creator>Hong, Zhen-feng</creator><creator>Chen, Zhi-qing</creator><creator>Wu, Jin-fa</creator><creator>Zhao, Jin-yan</creator><general>Chinese Association of Traditional and Western Medicine</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia</title><author>Huang, Yuan-peng ; Du, Jian ; Hong, Zhen-feng ; Chen, Zhi-qing ; Wu, Jin-fa ; Zhao, Jin-yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-e1856e5fbad0226a989f0652ffce814b674df83bdb6bcbd2e16606a5d0361d663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Body Weight - drug effects</topic><topic>Cell Proliferation - drug effects</topic><topic>Cookbooks as Topic</topic><topic>Drug Evaluation, Preclinical</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Drugs, Chinese Herbal - therapeutic use</topic><topic>Experimental Research</topic><topic>Gonadal Steroid Hormones - blood</topic><topic>Gonadal Steroid Hormones - metabolism</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Medicine, Chinese Traditional - methods</topic><topic>Organ Size - drug effects</topic><topic>Proliferating Cell Nuclear Antigen - genetics</topic><topic>Proliferating Cell Nuclear Antigen - metabolism</topic><topic>Prostate - drug effects</topic><topic>Prostate - pathology</topic><topic>Prostatic Hyperplasia - blood</topic><topic>Prostatic Hyperplasia - drug therapy</topic><topic>Prostatic Hyperplasia - metabolism</topic><topic>Prostatic Hyperplasia - pathology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Huang, Yuan-peng</creatorcontrib><creatorcontrib>Du, Jian</creatorcontrib><creatorcontrib>Hong, Zhen-feng</creatorcontrib><creatorcontrib>Chen, Zhi-qing</creatorcontrib><creatorcontrib>Wu, Jin-fa</creatorcontrib><creatorcontrib>Zhao, Jin-yan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Chinese journal of integrative medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Huang, Yuan-peng</au><au>Du, Jian</au><au>Hong, Zhen-feng</au><au>Chen, Zhi-qing</au><au>Wu, Jin-fa</au><au>Zhao, Jin-yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia</atitle><jtitle>Chinese journal of integrative medicine</jtitle><stitle>Chin. J. Integr. Med</stitle><addtitle>Chin J Integr Med</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>15</volume><issue>4</issue><spage>289</spage><epage>292</epage><pages>289-292</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective
To investigate the effects of Kangquan Recipe (康泉方, KQR) on sex steroids and cell proliferation in an experimental benign prostatic hyperplasia (BPH) model in rats.
Methods
Seventy-two SD rats were randomly divided into six groups: the normal group, the model group, the finasteride group, and the low-, middle-, and high-dose KQR groups, 12 in each group. Except those in the normal group, the rats were injected with testosterone after castration for the establishment of BPH model and then given respectively with normal saline, finasteride, and low-, middle-, and high-dose of KQR for 30 days. The levels of plasma testosterone (T) and estradiol (E
2
) were determined by enzyme-linked immunosorbent assay (ELISA), and the mRNA expression ) of proliferating cell nuclear antigen (PCNA) in prostate tissue was detected by reverse transcription-polymerase chain reaction (RT-PCR) after administration.
Results
Compared with the model group, the prostate weight, the plasma T, and the mRNA expression of PCNA were significantly lower, and the plasma E
2
and the ratio of E
2
/T were higher in the three KQR groups (
P
<0.05 or
P
<0.01). There was no significant difference in the prostate weight, plasma T and E
2
, and ratio of E
2
/T among the finasteride group and the three KQR groups (
P
>0.05). The mRNA expressions of PCNA were significantly higher in the middle- and low-dose of KQR groups than those in the finasteride group (
P
<0.05).
Conclusion
KQR shows multitarget effects on experimental BPH rats, and the mechanism might be related with regulating the balance of plasma T and E
2
and decreasing the PCNAmRNA expression in prostate tissue to restrain cell proliferation in a dose-dependent manner.</abstract><cop>Heidelberg</cop><pub>Chinese Association of Traditional and Western Medicine</pub><pmid>19688318</pmid><doi>10.1007/s11655-009-0289-3</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-0415 |
| ispartof | Chinese journal of integrative medicine, 2009-08, Vol.15 (4), p.289-292 |
| issn | 1672-0415 1993-0402 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733943018 |
| source | MEDLINE; SpringerLink Journals; Alma/SFX Local Collection |
| subjects | Animals Body Weight - drug effects Cell Proliferation - drug effects Cookbooks as Topic Drug Evaluation, Preclinical Drugs, Chinese Herbal - pharmacology Drugs, Chinese Herbal - therapeutic use Experimental Research Gonadal Steroid Hormones - blood Gonadal Steroid Hormones - metabolism Male Medicine Medicine & Public Health Medicine, Chinese Traditional - methods Organ Size - drug effects Proliferating Cell Nuclear Antigen - genetics Proliferating Cell Nuclear Antigen - metabolism Prostate - drug effects Prostate - pathology Prostatic Hyperplasia - blood Prostatic Hyperplasia - drug therapy Prostatic Hyperplasia - metabolism Prostatic Hyperplasia - pathology Rats Rats, Sprague-Dawley |
| title | Effects of Kangquan Recipe (康泉方) on sex steroids and cell proliferation in rats with benign prostatic hyperplasia |
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