Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration
The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is used a...
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| Veröffentlicht in: | Bioresource technology 2009-12, Vol.100 (23), p.5566-5573 |
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| creator | Van Hecke, Wouter Salaheddin, Clara Ludwig, Roland Dewulf, Jo Haltrich, Dietmar Langenhove, Herman Van |
| description | The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is used as electron acceptor for pyranose 2-oxidase because it shows more favourable kinetics in comparison to oxygen. The reduced redox mediator is in turn re-oxidized by laccase, which uses oxygen as the terminal electron acceptor, reducing it fully to water. However, pyranose 2-oxidase is capable of using oxygen as an electron acceptor in a competing side reaction, leading to the formation of hydrogen peroxide, which is detrimental for both enzymes and seriously limits the operational stability of both enzymes.
The experimental results showed full conversion of the aldose to the 2-ketoaldose and a good agreement with the simulations of the process. |
| doi_str_mv | 10.1016/j.biortech.2009.06.032 |
| format | Article |
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The experimental results showed full conversion of the aldose to the 2-ketoaldose and a good agreement with the simulations of the process.</description><subject>2-Ketoaldose</subject><subject>Adsorption</subject><subject>Benzoquinones - chemistry</subject><subject>Biocatalysis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Biotechnology - methods</subject><subject>Biotransformation</subject><subject>Carbohydrate Dehydrogenases - chemistry</subject><subject>Charcoal - chemistry</subject><subject>Chemistry - methods</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Kinetics</subject><subject>Laccase</subject><subject>Laccase - chemistry</subject><subject>Models, Chemical</subject><subject>Oxidation-Reduction</subject><subject>Oxygen - chemistry</subject><subject>Pyranose 2-oxidase</subject><subject>Reaction engineering</subject><subject>Redox mediator</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2P0zAQhi0EYsvCX1hyAU4JM3bixDdgxZe0EkiwZ8sfk-IqjYudIvrvcbcFbnAafzzvzOhh7AqhQUD5ctPYENNC7lvDAVQDsgHB77EVDr2ouerlfbYCJaEeOt5esEc5bwBAYM8fsgtUneq6Qa3Y5zchOrOY6bAEVzmTnfFUxZ_BmyXEudrnMK-rybjyRdUYU7U7JDPHcuH1HVZOidY0U7pLPGYPRjNlenKul-z23duv1x_qm0_vP16_vqld2_Gllr2VaJVXxrcCOy4GbiVZ4WU32K7U8mzBS4VtZ72zIAdwgN73XJmyubhkL059dyl-31Ne9DZkR9NkZor7rHshVMuHFgv5_J8kRxQC2qGA8gS6FHNONOpdCluTDhpBH63rjf5tXR-ta5C6WC_Bq_OEvd2S_xs7ay7AszNwFDyNxaAL-Q_HUXElZFu4pyduNFGbdSrM7RcOKMrwXiAeiVcngorbH4GSzi7Q7MiHRG7RPob_bfsLstKs4g</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Van Hecke, Wouter</creator><creator>Salaheddin, Clara</creator><creator>Ludwig, Roland</creator><creator>Dewulf, Jo</creator><creator>Haltrich, Dietmar</creator><creator>Langenhove, Herman Van</creator><general>Elsevier Ltd</general><general>[New York, NY]: Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7ST</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>20091201</creationdate><title>Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration</title><author>Van Hecke, Wouter ; Salaheddin, Clara ; Ludwig, Roland ; Dewulf, Jo ; Haltrich, Dietmar ; Langenhove, Herman Van</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-67b61b9d9ad43152382b6eb3d658b5b3dd43b0d69145bdcb0680c01dd729a5893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>2-Ketoaldose</topic><topic>Adsorption</topic><topic>Benzoquinones - chemistry</topic><topic>Biocatalysis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Biotechnology - methods</topic><topic>Biotransformation</topic><topic>Carbohydrate Dehydrogenases - chemistry</topic><topic>Charcoal - chemistry</topic><topic>Chemistry - methods</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Kinetics</topic><topic>Laccase</topic><topic>Laccase - chemistry</topic><topic>Models, Chemical</topic><topic>Oxidation-Reduction</topic><topic>Oxygen - chemistry</topic><topic>Pyranose 2-oxidase</topic><topic>Reaction engineering</topic><topic>Redox mediator</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Van Hecke, Wouter</creatorcontrib><creatorcontrib>Salaheddin, Clara</creatorcontrib><creatorcontrib>Ludwig, Roland</creatorcontrib><creatorcontrib>Dewulf, Jo</creatorcontrib><creatorcontrib>Haltrich, Dietmar</creatorcontrib><creatorcontrib>Langenhove, Herman Van</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Van Hecke, Wouter</au><au>Salaheddin, Clara</au><au>Ludwig, Roland</au><au>Dewulf, Jo</au><au>Haltrich, Dietmar</au><au>Langenhove, Herman Van</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>100</volume><issue>23</issue><spage>5566</spage><epage>5573</epage><pages>5566-5573</pages><issn>0960-8524</issn><eissn>1873-2976</eissn><abstract>The interactions between two oxidoreductases coupled by an artificial redox mediator have been described quantitatively to increase both stability and productivity. In this cascade oxidation, pyranose 2-oxidase oxidizes several aldoses at the C-2 position to 2-ketoaldoses. A redox mediator is used as electron acceptor for pyranose 2-oxidase because it shows more favourable kinetics in comparison to oxygen. The reduced redox mediator is in turn re-oxidized by laccase, which uses oxygen as the terminal electron acceptor, reducing it fully to water. However, pyranose 2-oxidase is capable of using oxygen as an electron acceptor in a competing side reaction, leading to the formation of hydrogen peroxide, which is detrimental for both enzymes and seriously limits the operational stability of both enzymes.
The experimental results showed full conversion of the aldose to the 2-ketoaldose and a good agreement with the simulations of the process.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19595589</pmid><doi>10.1016/j.biortech.2009.06.032</doi><tpages>8</tpages></addata></record> |
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| subjects | 2-Ketoaldose Adsorption Benzoquinones - chemistry Biocatalysis Biological and medical sciences Biotechnology Biotechnology - methods Biotransformation Carbohydrate Dehydrogenases - chemistry Charcoal - chemistry Chemistry - methods Chromatography, High Pressure Liquid - methods Fundamental and applied biological sciences. Psychology Hydrogen Peroxide - chemistry Kinetics Laccase Laccase - chemistry Models, Chemical Oxidation-Reduction Oxygen - chemistry Pyranose 2-oxidase Reaction engineering Redox mediator |
| title | Biocatalytic cascade oxidation using laccase for pyranose 2-oxidase regeneration |
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