Intrastriatal grafts of mesenchymal stem cells in adult intact rats can elevate tyrosine hydroxylase expression and dopamine levels
MSCs (mesenchymal stem cells) derived from the bone marrow have shown to be a promising source of stem cells in a therapeutic strategy of neurodegenerative disorder. Also, MSCs can enhance the TH (tyrosine hydroxylase) expression and DA (dopamine) content in catecholaminergic cells by in vitro co‐cu...
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| Veröffentlicht in: | Cell biology international 2010-01, Vol.34 (1), p.135-140 |
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| creator | Jin, Guang-Zhen Cho, Su-Jin Lee, Young-S Kim, Myeong-Ok Cho, Dong-Woo Kong, Il-Keun |
| description | MSCs (mesenchymal stem cells) derived from the bone marrow have shown to be a promising source of stem cells in a therapeutic strategy of neurodegenerative disorder. Also, MSCs can enhance the TH (tyrosine hydroxylase) expression and DA (dopamine) content in catecholaminergic cells by in vitro co‐culture system. In the present study, we investigated the effect of intrastriatal grafts of MSCs on TH protein and gene levels and DA content in adult intact rats. When MSCs were transplanted into the striatum of normal rats, the grafted striatum not only had significantly higher TH protein and mRNA levels, but also significantly higher DA content than the untransplanted striatum. Meanwhile, the grafted MSCs differentiated into neurons, astrocytes and oligodendrocytes; however, TH‐positive cells could not be detected in our study. These experimental results offer further evidence that MSCs are a promising candidate for treating neurodegenerative diseases such as Parkinson's disease. |
| doi_str_mv | 10.1042/CBI20090012 |
| format | Article |
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These experimental results offer further evidence that MSCs are a promising candidate for treating neurodegenerative diseases such as Parkinson's disease.</description><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Cell Differentiation</subject><subject>Corpus Striatum</subject><subject>dopamine</subject><subject>Dopamine - metabolism</subject><subject>Immunohistochemistry</subject><subject>Mesenchymal Stem Cell Transplantation</subject><subject>mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - metabolism</subject><subject>Rats</subject><subject>RNA, Messenger - metabolism</subject><subject>transplantation</subject><subject>Tyrosine 3-Monooxygenase - genetics</subject><subject>Tyrosine 3-Monooxygenase - metabolism</subject><subject>tyrosine hydroxylase</subject><issn>1065-6995</issn><issn>1095-8355</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM9vFCEYhonR2Fo9eTfcPJipMMAAR930xyZN7UFj0gth4cOOMjMrsLpz9h-XyW7UkyfekOd5k-9F6CUl55Tw9u3q_bolRBNC20folBItGsWEeLzkTjSd1uIEPcv5ayUoV91TdEK15lLz7hT9Wo8l2VxSb4uN-EuyoWQ8BTxAhtE9zEP9zQUG7CDGjPsRW7-LpYZiXcHJVtzZEUOEH7YALnOacj8Cfph9mvZztBkw7LcJcu6nao8e-2lrh4WpDsT8HD0JNmZ4cXzP0KfLi4-r6-bmw9V69e6mcaxTrFE8uFZYoCSEDXhJvdoo1SrpNRfcei-EU4FzKWxwjgoHQmipFO2E6BxwdoZeH3q3afq-g1zM0OflLDvCtMtGMqaZ1q2q5JsD6eoxOUEw29QPNs2GErOMbv4ZvdKvjr27zQD-L3tcuQL0APzsI8z_61ryLWspq05zcPq6_v6PY9M300kmhfl8e2XuLu-l4vfK3LHfzA6dHg</recordid><startdate>201001</startdate><enddate>201001</enddate><creator>Jin, Guang-Zhen</creator><creator>Cho, Su-Jin</creator><creator>Lee, Young-S</creator><creator>Kim, Myeong-Ok</creator><creator>Cho, Dong-Woo</creator><creator>Kong, Il-Keun</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201001</creationdate><title>Intrastriatal grafts of mesenchymal stem cells in adult intact rats can elevate tyrosine hydroxylase expression and dopamine levels</title><author>Jin, Guang-Zhen ; Cho, Su-Jin ; Lee, Young-S ; Kim, Myeong-Ok ; Cho, Dong-Woo ; Kong, Il-Keun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3683-84fc25ae10ffbed71d8b88287d9454add55c8f4475afcc15ce55978816556ce43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Cell Differentiation</topic><topic>Corpus Striatum</topic><topic>dopamine</topic><topic>Dopamine - metabolism</topic><topic>Immunohistochemistry</topic><topic>Mesenchymal Stem Cell Transplantation</topic><topic>mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - metabolism</topic><topic>Rats</topic><topic>RNA, Messenger - metabolism</topic><topic>transplantation</topic><topic>Tyrosine 3-Monooxygenase - genetics</topic><topic>Tyrosine 3-Monooxygenase - metabolism</topic><topic>tyrosine hydroxylase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jin, Guang-Zhen</creatorcontrib><creatorcontrib>Cho, Su-Jin</creatorcontrib><creatorcontrib>Lee, Young-S</creatorcontrib><creatorcontrib>Kim, Myeong-Ok</creatorcontrib><creatorcontrib>Cho, Dong-Woo</creatorcontrib><creatorcontrib>Kong, Il-Keun</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biology international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jin, Guang-Zhen</au><au>Cho, Su-Jin</au><au>Lee, Young-S</au><au>Kim, Myeong-Ok</au><au>Cho, Dong-Woo</au><au>Kong, Il-Keun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrastriatal grafts of mesenchymal stem cells in adult intact rats can elevate tyrosine hydroxylase expression and dopamine levels</atitle><jtitle>Cell biology international</jtitle><addtitle>Cell Biol Int</addtitle><date>2010-01</date><risdate>2010</risdate><volume>34</volume><issue>1</issue><spage>135</spage><epage>140</epage><pages>135-140</pages><issn>1065-6995</issn><eissn>1095-8355</eissn><abstract>MSCs (mesenchymal stem cells) derived from the bone marrow have shown to be a promising source of stem cells in a therapeutic strategy of neurodegenerative disorder. 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| ispartof | Cell biology international, 2010-01, Vol.34 (1), p.135-140 |
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| source | Wiley-Blackwell Journals; MEDLINE |
| subjects | Animals Bone Marrow Cells - cytology Cell Differentiation Corpus Striatum dopamine Dopamine - metabolism Immunohistochemistry Mesenchymal Stem Cell Transplantation mesenchymal stem cells Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - metabolism Rats RNA, Messenger - metabolism transplantation Tyrosine 3-Monooxygenase - genetics Tyrosine 3-Monooxygenase - metabolism tyrosine hydroxylase |
| title | Intrastriatal grafts of mesenchymal stem cells in adult intact rats can elevate tyrosine hydroxylase expression and dopamine levels |
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