Detection of Mycoplasma in cell cultures
Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell physiology, rendering experiments that are conducted with contaminated cells worthless. Because of their small size, Mycoplasmas can pass through filters use...
Gespeichert in:
| Veröffentlicht in: | Nature protocols 2010-05, Vol.5 (5), p.929-934 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 934 |
|---|---|
| container_issue | 5 |
| container_start_page | 929 |
| container_title | Nature protocols |
| container_volume | 5 |
| creator | Young, Lesley Sung, Julia Stacey, Glyn Masters, John R |
| description | Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell physiology, rendering experiments that are conducted with contaminated cells worthless. Because of their small size, Mycoplasmas can pass through filters used to prevent bacterial and fungal contamination and potentially spread to all the cultures in a laboratory. It is essential that all new cell cultures entering a laboratory and all cell banks are tested for the presence of Mycoplasma. It is recommended that two techniques be used, selected from a PCR-based method, indirect staining and an agar and broth culture. This protocol describes these three tests for detecting Mycoplasma, which take from 1 d to 3–4 weeks, and such tests should be an obligatory component of quality control in every tissue culture laboratory. |
| doi_str_mv | 10.1038/nprot.2010.43 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_733934172</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A284450644</galeid><sourcerecordid>A284450644</sourcerecordid><originalsourceid>FETCH-LOGICAL-c529t-238c17d3a1dd35c23b7faec53eb3b95252d3fc67eb16c225fcdd1de88fc375353</originalsourceid><addsrcrecordid>eNp90sFu1DAQBmALgWgpHLmiSBwoQllij71OjqulQKUCEhRxtBx7vEqVxFvbkejb19kWyqJCfEicfB7lHw0hz2m1oBXUb8dt8GnBqrzn8IAcUimqksmmebh75iWjdXNAnsR4UVVcwlI-Jges4kAF1Ifk-B0mNKnzY-Fd8enK-G2v46CLbiwM9n1hpj5NAeNT8sjpPuKz2_sR-f7-5Hz9sTz78uF0vTorjWBNKhnUhkoLmloLwjBopdNoBGALbSOYYBacWUps6dIwJpyxllqsa2dAChBwRF7d1M25LieMSQ1dnP9Ej-inqCRAA5xKluXxf-XcHtE0kteZvvyLXvgpjDnHrJgQMl93aqN7VN3ofArazEXVitWci2rJeVaLe1ReFofO-BFdl9_vHXi9dyCbhD_TRk8xqtNvX_ftm3_b1fmP9ed9Xd5oE3yMAZ3ahm7Q4Sqn2sVXu-lQ83QoDtm_uG3D1A5of-tf43CXLeZP4wbDn326r-I1DHDAGA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1032557777</pqid></control><display><type>article</type><title>Detection of Mycoplasma in cell cultures</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><source>Nature Journals Online</source><creator>Young, Lesley ; Sung, Julia ; Stacey, Glyn ; Masters, John R</creator><creatorcontrib>Young, Lesley ; Sung, Julia ; Stacey, Glyn ; Masters, John R</creatorcontrib><description>Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell physiology, rendering experiments that are conducted with contaminated cells worthless. Because of their small size, Mycoplasmas can pass through filters used to prevent bacterial and fungal contamination and potentially spread to all the cultures in a laboratory. It is essential that all new cell cultures entering a laboratory and all cell banks are tested for the presence of Mycoplasma. It is recommended that two techniques be used, selected from a PCR-based method, indirect staining and an agar and broth culture. This protocol describes these three tests for detecting Mycoplasma, which take from 1 d to 3–4 weeks, and such tests should be an obligatory component of quality control in every tissue culture laboratory.</description><identifier>ISSN: 1754-2189</identifier><identifier>EISSN: 1750-2799</identifier><identifier>DOI: 10.1038/nprot.2010.43</identifier><identifier>PMID: 20431538</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/1647/1407/651 ; 631/326/41 ; Analytical Chemistry ; Antibiotics ; Bacteriological Techniques - methods ; Banks (Finance) ; Biological Techniques ; Biomedical and Life Sciences ; Cell culture ; Computational Biology/Bioinformatics ; Contaminants ; DNA, Bacterial ; Electrophoresis, Agar Gel ; Filters ; Laboratories ; Life Sciences ; Microarrays ; Mycoplasma ; Mycoplasma - isolation & purification ; Organic Chemistry ; Organisms ; Physiology ; Polymerase Chain Reaction - methods ; Protocol ; Quality control ; Sensitivity and Specificity ; Staining and Labeling - methods ; Stains & staining ; Stem cells ; Time Factors</subject><ispartof>Nature protocols, 2010-05, Vol.5 (5), p.929-934</ispartof><rights>Springer Nature Limited 2010</rights><rights>COPYRIGHT 2010 Nature Publishing Group</rights><rights>Copyright Nature Publishing Group May 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c529t-238c17d3a1dd35c23b7faec53eb3b95252d3fc67eb16c225fcdd1de88fc375353</citedby><cites>FETCH-LOGICAL-c529t-238c17d3a1dd35c23b7faec53eb3b95252d3fc67eb16c225fcdd1de88fc375353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/nprot.2010.43$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/nprot.2010.43$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20431538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Young, Lesley</creatorcontrib><creatorcontrib>Sung, Julia</creatorcontrib><creatorcontrib>Stacey, Glyn</creatorcontrib><creatorcontrib>Masters, John R</creatorcontrib><title>Detection of Mycoplasma in cell cultures</title><title>Nature protocols</title><addtitle>Nat Protoc</addtitle><addtitle>Nat Protoc</addtitle><description>Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell physiology, rendering experiments that are conducted with contaminated cells worthless. Because of their small size, Mycoplasmas can pass through filters used to prevent bacterial and fungal contamination and potentially spread to all the cultures in a laboratory. It is essential that all new cell cultures entering a laboratory and all cell banks are tested for the presence of Mycoplasma. It is recommended that two techniques be used, selected from a PCR-based method, indirect staining and an agar and broth culture. This protocol describes these three tests for detecting Mycoplasma, which take from 1 d to 3–4 weeks, and such tests should be an obligatory component of quality control in every tissue culture laboratory.</description><subject>631/1647/1407/651</subject><subject>631/326/41</subject><subject>Analytical Chemistry</subject><subject>Antibiotics</subject><subject>Bacteriological Techniques - methods</subject><subject>Banks (Finance)</subject><subject>Biological Techniques</subject><subject>Biomedical and Life Sciences</subject><subject>Cell culture</subject><subject>Computational Biology/Bioinformatics</subject><subject>Contaminants</subject><subject>DNA, Bacterial</subject><subject>Electrophoresis, Agar Gel</subject><subject>Filters</subject><subject>Laboratories</subject><subject>Life Sciences</subject><subject>Microarrays</subject><subject>Mycoplasma</subject><subject>Mycoplasma - isolation & purification</subject><subject>Organic Chemistry</subject><subject>Organisms</subject><subject>Physiology</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Protocol</subject><subject>Quality control</subject><subject>Sensitivity and Specificity</subject><subject>Staining and Labeling - methods</subject><subject>Stains & staining</subject><subject>Stem cells</subject><subject>Time Factors</subject><issn>1754-2189</issn><issn>1750-2799</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp90sFu1DAQBmALgWgpHLmiSBwoQllij71OjqulQKUCEhRxtBx7vEqVxFvbkejb19kWyqJCfEicfB7lHw0hz2m1oBXUb8dt8GnBqrzn8IAcUimqksmmebh75iWjdXNAnsR4UVVcwlI-Jges4kAF1Ifk-B0mNKnzY-Fd8enK-G2v46CLbiwM9n1hpj5NAeNT8sjpPuKz2_sR-f7-5Hz9sTz78uF0vTorjWBNKhnUhkoLmloLwjBopdNoBGALbSOYYBacWUps6dIwJpyxllqsa2dAChBwRF7d1M25LieMSQ1dnP9Ej-inqCRAA5xKluXxf-XcHtE0kteZvvyLXvgpjDnHrJgQMl93aqN7VN3ofArazEXVitWci2rJeVaLe1ReFofO-BFdl9_vHXi9dyCbhD_TRk8xqtNvX_ftm3_b1fmP9ed9Xd5oE3yMAZ3ahm7Q4Sqn2sVXu-lQ83QoDtm_uG3D1A5of-tf43CXLeZP4wbDn326r-I1DHDAGA</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Young, Lesley</creator><creator>Sung, Julia</creator><creator>Stacey, Glyn</creator><creator>Masters, John R</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ATWCN</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PATMY</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7QL</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>201005</creationdate><title>Detection of Mycoplasma in cell cultures</title><author>Young, Lesley ; Sung, Julia ; Stacey, Glyn ; Masters, John R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c529t-238c17d3a1dd35c23b7faec53eb3b95252d3fc67eb16c225fcdd1de88fc375353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>631/1647/1407/651</topic><topic>631/326/41</topic><topic>Analytical Chemistry</topic><topic>Antibiotics</topic><topic>Bacteriological Techniques - methods</topic><topic>Banks (Finance)</topic><topic>Biological Techniques</topic><topic>Biomedical and Life Sciences</topic><topic>Cell culture</topic><topic>Computational Biology/Bioinformatics</topic><topic>Contaminants</topic><topic>DNA, Bacterial</topic><topic>Electrophoresis, Agar Gel</topic><topic>Filters</topic><topic>Laboratories</topic><topic>Life Sciences</topic><topic>Microarrays</topic><topic>Mycoplasma</topic><topic>Mycoplasma - isolation & purification</topic><topic>Organic Chemistry</topic><topic>Organisms</topic><topic>Physiology</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Protocol</topic><topic>Quality control</topic><topic>Sensitivity and Specificity</topic><topic>Staining and Labeling - methods</topic><topic>Stains & staining</topic><topic>Stem cells</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Young, Lesley</creatorcontrib><creatorcontrib>Sung, Julia</creatorcontrib><creatorcontrib>Stacey, Glyn</creatorcontrib><creatorcontrib>Masters, John R</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Middle School</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Nature protocols</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Young, Lesley</au><au>Sung, Julia</au><au>Stacey, Glyn</au><au>Masters, John R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Detection of Mycoplasma in cell cultures</atitle><jtitle>Nature protocols</jtitle><stitle>Nat Protoc</stitle><addtitle>Nat Protoc</addtitle><date>2010-05</date><risdate>2010</risdate><volume>5</volume><issue>5</issue><spage>929</spage><epage>934</epage><pages>929-934</pages><issn>1754-2189</issn><eissn>1750-2799</eissn><abstract>Mycoplasma is a prokaryotic organism that is a frequent and occult contaminant of cell cultures. This organism can modify many aspects of cell physiology, rendering experiments that are conducted with contaminated cells worthless. Because of their small size, Mycoplasmas can pass through filters used to prevent bacterial and fungal contamination and potentially spread to all the cultures in a laboratory. It is essential that all new cell cultures entering a laboratory and all cell banks are tested for the presence of Mycoplasma. It is recommended that two techniques be used, selected from a PCR-based method, indirect staining and an agar and broth culture. This protocol describes these three tests for detecting Mycoplasma, which take from 1 d to 3–4 weeks, and such tests should be an obligatory component of quality control in every tissue culture laboratory.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>20431538</pmid><doi>10.1038/nprot.2010.43</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1754-2189 |
| ispartof | Nature protocols, 2010-05, Vol.5 (5), p.929-934 |
| issn | 1754-2189 1750-2799 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733934172 |
| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | 631/1647/1407/651 631/326/41 Analytical Chemistry Antibiotics Bacteriological Techniques - methods Banks (Finance) Biological Techniques Biomedical and Life Sciences Cell culture Computational Biology/Bioinformatics Contaminants DNA, Bacterial Electrophoresis, Agar Gel Filters Laboratories Life Sciences Microarrays Mycoplasma Mycoplasma - isolation & purification Organic Chemistry Organisms Physiology Polymerase Chain Reaction - methods Protocol Quality control Sensitivity and Specificity Staining and Labeling - methods Stains & staining Stem cells Time Factors |
| title | Detection of Mycoplasma in cell cultures |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-20T20%3A54%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Detection%20of%20Mycoplasma%20in%20cell%20cultures&rft.jtitle=Nature%20protocols&rft.au=Young,%20Lesley&rft.date=2010-05&rft.volume=5&rft.issue=5&rft.spage=929&rft.epage=934&rft.pages=929-934&rft.issn=1754-2189&rft.eissn=1750-2799&rft_id=info:doi/10.1038/nprot.2010.43&rft_dat=%3Cgale_proqu%3EA284450644%3C/gale_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1032557777&rft_id=info:pmid/20431538&rft_galeid=A284450644&rfr_iscdi=true |