A sample preparation protocol for 1H nuclear magnetic resonance studies of water-soluble metabolites in blood and urine
We describe a general protocol for preparing protein-containing biofluids for 1H nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference com...
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| Veröffentlicht in: | Analytical biochemistry 2010-03, Vol.398 (2), p.263-265 |
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| container_title | Analytical biochemistry |
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| creator | Sheedy, John R. Ebeling, Peter R. Gooley, Paul R. McConville, Malcolm J. |
| description | We describe a general protocol for preparing protein-containing biofluids for
1H nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference compounds such as 3-trimethylsilylpropionic acid (TSP) and 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS). The efficacy of this protocol was tested using a bovine serum albumin/metabolite mix and human serum samples. This sample preparation method can be readily applied to any protein-containing biofluid for
1H NMR studies. |
| doi_str_mv | 10.1016/j.ab.2009.11.027 |
| format | Article |
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1H nuclear magnetic resonance (NMR) metabolomic studies. In this protocol, untreated samples are diluted in deuterated solvents to precipitate proteins and recover metabolites quantitated relative to standard reference compounds such as 3-trimethylsilylpropionic acid (TSP) and 2,2-dimethyl-2-silapentane-5-sulfonic acid (DSS). The efficacy of this protocol was tested using a bovine serum albumin/metabolite mix and human serum samples. This sample preparation method can be readily applied to any protein-containing biofluid for
1H NMR studies.</description><subject>Analytic Sample Preparation Methods - methods</subject><subject>Animals</subject><subject>Biofluids</subject><subject>Blood Chemical Analysis - methods</subject><subject>Cattle</subject><subject>Humans</subject><subject>Magnetic Resonance Spectroscopy</subject><subject>Metabolites</subject><subject>NMR</subject><subject>Proteins - analysis</subject><subject>Proteins - isolation & purification</subject><subject>Proteins - metabolism</subject><subject>Solubility</subject><subject>Urinalysis - methods</subject><subject>Water - chemistry</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM2LFDEQxYMo7uzq3ZPk5qnbVCf95W1Z1F1Y8KLnUJ1US4Z0MiZpF_97s8yAJ09VUO896v0YeweiBQHDx2OLS9sJMbcArejGF-wAYh4aIcX8kh2EELLphnm8Ytc5H4UAUP3wml3BPCuYJBzY0y3PuJ088VOiEyYsLoa6xxJN9HyNicM9D7vxhIlv-DNQcYYnyjFgMMRz2a2jzOPKn7BQanL0-1LzNiq4RO9KPbrAFx-j5Rgs35ML9Ia9WtFnenuZN-zHl8_f7-6bx29fH-5uHxsD9cXGgpJo-3VchRp7rO_3curJTtgNKHFVCrpusQZwwnHq-rkDO3RSqhkJUAl5wz6cc2ulXzvlojeXDXmPgeKe9SjlLGEcVVWKs9KkmHOiVZ-S2zD90SD0M2191LjoZ9oaQFfa1fL-Er4vG9l_hgveKvh0FlCt-NtR0tk4qtisS2SKttH9P_0v-WaPhA</recordid><startdate>20100315</startdate><enddate>20100315</enddate><creator>Sheedy, John R.</creator><creator>Ebeling, Peter R.</creator><creator>Gooley, Paul R.</creator><creator>McConville, Malcolm J.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100315</creationdate><title>A sample preparation protocol for 1H nuclear magnetic resonance studies of water-soluble metabolites in blood and urine</title><author>Sheedy, John R. ; Ebeling, Peter R. ; Gooley, Paul R. ; McConville, Malcolm J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1941-d143ad5f7f0475a1455385ed8a26a3af44122bdc1a8a7825921d623349ae1a403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytic Sample Preparation Methods - methods</topic><topic>Animals</topic><topic>Biofluids</topic><topic>Blood Chemical Analysis - methods</topic><topic>Cattle</topic><topic>Humans</topic><topic>Magnetic Resonance Spectroscopy</topic><topic>Metabolites</topic><topic>NMR</topic><topic>Proteins - analysis</topic><topic>Proteins - isolation & purification</topic><topic>Proteins - metabolism</topic><topic>Solubility</topic><topic>Urinalysis - methods</topic><topic>Water - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sheedy, John R.</creatorcontrib><creatorcontrib>Ebeling, Peter R.</creatorcontrib><creatorcontrib>Gooley, Paul R.</creatorcontrib><creatorcontrib>McConville, Malcolm J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sheedy, John R.</au><au>Ebeling, Peter R.</au><au>Gooley, Paul R.</au><au>McConville, Malcolm J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A sample preparation protocol for 1H nuclear magnetic resonance studies of water-soluble metabolites in blood and urine</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>2010-03-15</date><risdate>2010</risdate><volume>398</volume><issue>2</issue><spage>263</spage><epage>265</epage><pages>263-265</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>We describe a general protocol for preparing protein-containing biofluids for
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| ispartof | Analytical biochemistry, 2010-03, Vol.398 (2), p.263-265 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analytic Sample Preparation Methods - methods Animals Biofluids Blood Chemical Analysis - methods Cattle Humans Magnetic Resonance Spectroscopy Metabolites NMR Proteins - analysis Proteins - isolation & purification Proteins - metabolism Solubility Urinalysis - methods Water - chemistry |
| title | A sample preparation protocol for 1H nuclear magnetic resonance studies of water-soluble metabolites in blood and urine |
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