Differential expression of new splice variants of the neurotensin receptor 1 gene in human prostate cancer cell lines
Neurotensin is a neuroendocrine peptide acting as a trophic factor in a variety of cells in vivo but it can also function as an autocrine growth factor in human prostate cancer cells in vitro. In addition, the high-affinity G protein-coupled NT receptor (NTS1) is overexpressed in prostate cancer cel...
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| Veröffentlicht in: | Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2010-02, Vol.31 (2), p.242-247 |
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| container_title | Peptides (New York, N.Y. : 1980) |
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| creator | Almeida, Teresa A. Rodriguez, Yurena Hernández, Mariano Reyes, Ricardo Bello, Aixa R. |
| description | Neurotensin is a neuroendocrine peptide acting as a trophic factor in a variety of cells in vivo but it can also function as an autocrine growth factor in human prostate cancer cells in vitro. In addition, the high-affinity G protein-coupled NT receptor (NTS1) is overexpressed in prostate cancer cell lines. Increasing evidence argues for a direct correlation between specific alternative splice variants and cancer. We detected four splice variants of the NTS1 receptor in human prostate cancer cell lines. These isoforms include one or more exons skipping as well as an alternative 5′ splice donor site and are expressed in the late-stage androgen independent prostate cancer cell lines PC3 and DU145, but not in the early-stage androgen-sensitive LNCaP or in normal prostate tissue, which only express the normal transcript. This result shows new splice variants of NTS1 for the first time. The differential expression observed among prostate cancer cell lines and normal prostate tissue opens the interesting possibility of a new role of NT/NTS1 pathway in prostate cancer. |
| doi_str_mv | 10.1016/j.peptides.2009.12.007 |
| format | Article |
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In addition, the high-affinity G protein-coupled NT receptor (NTS1) is overexpressed in prostate cancer cell lines. Increasing evidence argues for a direct correlation between specific alternative splice variants and cancer. We detected four splice variants of the NTS1 receptor in human prostate cancer cell lines. These isoforms include one or more exons skipping as well as an alternative 5′ splice donor site and are expressed in the late-stage androgen independent prostate cancer cell lines PC3 and DU145, but not in the early-stage androgen-sensitive LNCaP or in normal prostate tissue, which only express the normal transcript. This result shows new splice variants of NTS1 for the first time. The differential expression observed among prostate cancer cell lines and normal prostate tissue opens the interesting possibility of a new role of NT/NTS1 pathway in prostate cancer.</description><identifier>ISSN: 0196-9781</identifier><identifier>EISSN: 1873-5169</identifier><identifier>DOI: 10.1016/j.peptides.2009.12.007</identifier><identifier>PMID: 20018219</identifier><identifier>CODEN: PPTDD5</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Alternative splicing ; Amino Acid Sequence ; Biological and medical sciences ; Cancer ; Cell Line, Tumor ; Cloning, Molecular ; Codon, Terminator - genetics ; DNA Primers - genetics ; Exons - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Expression - genetics ; Gynecology. Andrology. Obstetrics ; Humans ; Male ; Male genital diseases ; Medical sciences ; Molecular Sequence Data ; Nephrology. Urinary tract diseases ; Neurotensin ; Neurotensin receptor 1 ; Prostatic Neoplasms - metabolism ; Protein Isoforms - genetics ; Receptors, Neurotensin - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Splice Sites - genetics ; RNA, Messenger - genetics ; RT-PCR ; Sequence Alignment ; Tumors ; Tumors of the urinary system ; Urinary tract. Prostate gland ; Vertebrates: endocrinology</subject><ispartof>Peptides (New York, N.Y. : 1980), 2010-02, Vol.31 (2), p.242-247</ispartof><rights>2009 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><rights>(c) 2009 Elsevier Inc. 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In addition, the high-affinity G protein-coupled NT receptor (NTS1) is overexpressed in prostate cancer cell lines. Increasing evidence argues for a direct correlation between specific alternative splice variants and cancer. We detected four splice variants of the NTS1 receptor in human prostate cancer cell lines. These isoforms include one or more exons skipping as well as an alternative 5′ splice donor site and are expressed in the late-stage androgen independent prostate cancer cell lines PC3 and DU145, but not in the early-stage androgen-sensitive LNCaP or in normal prostate tissue, which only express the normal transcript. This result shows new splice variants of NTS1 for the first time. The differential expression observed among prostate cancer cell lines and normal prostate tissue opens the interesting possibility of a new role of NT/NTS1 pathway in prostate cancer.</description><subject>Alternative splicing</subject><subject>Amino Acid Sequence</subject><subject>Biological and medical sciences</subject><subject>Cancer</subject><subject>Cell Line, Tumor</subject><subject>Cloning, Molecular</subject><subject>Codon, Terminator - genetics</subject><subject>DNA Primers - genetics</subject><subject>Exons - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - genetics</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Male</subject><subject>Male genital diseases</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Nephrology. Urinary tract diseases</subject><subject>Neurotensin</subject><subject>Neurotensin receptor 1</subject><subject>Prostatic Neoplasms - metabolism</subject><subject>Protein Isoforms - genetics</subject><subject>Receptors, Neurotensin - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Splice Sites - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RT-PCR</subject><subject>Sequence Alignment</subject><subject>Tumors</subject><subject>Tumors of the urinary system</subject><subject>Urinary tract. Prostate gland</subject><subject>Vertebrates: endocrinology</subject><issn>0196-9781</issn><issn>1873-5169</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1v1DAQhi0EokvhL1S-IE4JHie24xuofEqVuMDZcp0J9SrrBI9T4N_j1W7hyGmk8fOOXz2MXYFoQYB-vW9XXEsckVophG1BtkKYR2wHg-kaBdo-ZjsBVjfWDHDBnhHthRB9b4en7KJGYJBgd2x7F6cJM6YS_czx15qRKC6JLxNP-JPTOseA_N7n6FOh47rcYX3a8lIwUUw8Y6hVlsyBf8eEvK7utoNPfM0LFV-QB58CZh5wnvkcE9Jz9mTyM-GL87xk3z68_3r9qbn58vHz9dubJnTWlMaOGAZABZ0CP2kzeaxDjzIYpUUPEHSvVd91aJQU-ha9BjmYgKOyQ1Bjd8lene7WKj82pOIOkY41fMJlI2e6zkqttKqkPpGhlqaMk1tzPPj824FwR-Nu7x6Mu6NxB9JV4zV4df5iuz3g-Df2oLgCL8-Ap-DnKVcZkf5xsjPQK1G5NycOq5D7iNlRiFjFjbEaLm5c4v-6_AGky6Q6</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Almeida, Teresa A.</creator><creator>Rodriguez, Yurena</creator><creator>Hernández, Mariano</creator><creator>Reyes, Ricardo</creator><creator>Bello, Aixa R.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>Differential expression of new splice variants of the neurotensin receptor 1 gene in human prostate cancer cell lines</title><author>Almeida, Teresa A. ; Rodriguez, Yurena ; Hernández, Mariano ; Reyes, Ricardo ; Bello, Aixa R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-9dec81e51351af67faeaf66d2c7560411c6465433e75206bea61287ced598c5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Alternative splicing</topic><topic>Amino Acid Sequence</topic><topic>Biological and medical sciences</topic><topic>Cancer</topic><topic>Cell Line, Tumor</topic><topic>Cloning, Molecular</topic><topic>Codon, Terminator - genetics</topic><topic>DNA Primers - genetics</topic><topic>Exons - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - genetics</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Male</topic><topic>Male genital diseases</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Nephrology. Urinary tract diseases</topic><topic>Neurotensin</topic><topic>Neurotensin receptor 1</topic><topic>Prostatic Neoplasms - metabolism</topic><topic>Protein Isoforms - genetics</topic><topic>Receptors, Neurotensin - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Splice Sites - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RT-PCR</topic><topic>Sequence Alignment</topic><topic>Tumors</topic><topic>Tumors of the urinary system</topic><topic>Urinary tract. Prostate gland</topic><topic>Vertebrates: endocrinology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Almeida, Teresa A.</creatorcontrib><creatorcontrib>Rodriguez, Yurena</creatorcontrib><creatorcontrib>Hernández, Mariano</creatorcontrib><creatorcontrib>Reyes, Ricardo</creatorcontrib><creatorcontrib>Bello, Aixa R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Peptides (New York, N.Y. : 1980)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Almeida, Teresa A.</au><au>Rodriguez, Yurena</au><au>Hernández, Mariano</au><au>Reyes, Ricardo</au><au>Bello, Aixa R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential expression of new splice variants of the neurotensin receptor 1 gene in human prostate cancer cell lines</atitle><jtitle>Peptides (New York, N.Y. : 1980)</jtitle><addtitle>Peptides</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>31</volume><issue>2</issue><spage>242</spage><epage>247</epage><pages>242-247</pages><issn>0196-9781</issn><eissn>1873-5169</eissn><coden>PPTDD5</coden><abstract>Neurotensin is a neuroendocrine peptide acting as a trophic factor in a variety of cells in vivo but it can also function as an autocrine growth factor in human prostate cancer cells in vitro. In addition, the high-affinity G protein-coupled NT receptor (NTS1) is overexpressed in prostate cancer cell lines. Increasing evidence argues for a direct correlation between specific alternative splice variants and cancer. We detected four splice variants of the NTS1 receptor in human prostate cancer cell lines. These isoforms include one or more exons skipping as well as an alternative 5′ splice donor site and are expressed in the late-stage androgen independent prostate cancer cell lines PC3 and DU145, but not in the early-stage androgen-sensitive LNCaP or in normal prostate tissue, which only express the normal transcript. This result shows new splice variants of NTS1 for the first time. 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| ispartof | Peptides (New York, N.Y. : 1980), 2010-02, Vol.31 (2), p.242-247 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alternative splicing Amino Acid Sequence Biological and medical sciences Cancer Cell Line, Tumor Cloning, Molecular Codon, Terminator - genetics DNA Primers - genetics Exons - genetics Fundamental and applied biological sciences. Psychology Gene Expression - genetics Gynecology. Andrology. Obstetrics Humans Male Male genital diseases Medical sciences Molecular Sequence Data Nephrology. Urinary tract diseases Neurotensin Neurotensin receptor 1 Prostatic Neoplasms - metabolism Protein Isoforms - genetics Receptors, Neurotensin - genetics Reverse Transcriptase Polymerase Chain Reaction RNA Splice Sites - genetics RNA, Messenger - genetics RT-PCR Sequence Alignment Tumors Tumors of the urinary system Urinary tract. Prostate gland Vertebrates: endocrinology |
| title | Differential expression of new splice variants of the neurotensin receptor 1 gene in human prostate cancer cell lines |
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