In vitro heat shock of human monocytes results in a proportional increase of inducible Hsp70 expression according to the basal content
Heat shock proteins play an important role as molecular chaperones of the cell. Inducible heat shock protein 70 is rapidly synthesised in response to numerous stressors and monocytes are sensitive to changes in core temperature resulting in a circadian variation of Hsp70 expression. Monocytes were i...
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| Veröffentlicht in: | Amino acids 2010-05, Vol.38 (5), p.1423-1428 |
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| creator | Vince, Rebecca V Oliver, Katherine Midgley, Adrian W McNaughton, Lars R Madden, Leigh A |
| description | Heat shock proteins play an important role as molecular chaperones of the cell. Inducible heat shock protein 70 is rapidly synthesised in response to numerous stressors and monocytes are sensitive to changes in core temperature resulting in a circadian variation of Hsp70 expression. Monocytes were isolated via density centrifugation from nine healthy male volunteers at 5 am, 1 pm and 9 pm, representing the nadir (5 am), peak (9 pm) and intermediate (1 pm) of Hsp70 expression in the 24-h cycle. Analysis of freshly isolated monocytes for Hsp70 expression confirmed Hsp70 levels at the three selected time points. Monocytes were subjected to in vitro heat shock at 40°C (±0.1) for 90 min with a 90 min 37°C (±0.1) exposure acting as a control. A significant increase in Hsp70 was observed at 5 am (p < 0.001) and 1 pm (p = 0.028) at 40°C when compared to 37°C but not at 9 pm (p = 0.19). A significant increase was also observed from the basal levels of Hsp70, measured on freshly isolated monocytes and the levels detected after heat shock at 40°C at 5 am (p < 0.001) and 1 pm (p = 0.001), which was not observed at 9 pm (p = 0.15). Furthermore, a significant correlation was observed in the heat shock response at 40°C and that obtained at 37°C (p < 0.001). In conclusion, the heat shock response in monocytes is directly proportional to the amount of Hsp70 present in the cells and the stress response may be much higher at different times of the day. |
| doi_str_mv | 10.1007/s00726-009-0354-4 |
| format | Article |
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Inducible heat shock protein 70 is rapidly synthesised in response to numerous stressors and monocytes are sensitive to changes in core temperature resulting in a circadian variation of Hsp70 expression. Monocytes were isolated via density centrifugation from nine healthy male volunteers at 5 am, 1 pm and 9 pm, representing the nadir (5 am), peak (9 pm) and intermediate (1 pm) of Hsp70 expression in the 24-h cycle. Analysis of freshly isolated monocytes for Hsp70 expression confirmed Hsp70 levels at the three selected time points. Monocytes were subjected to in vitro heat shock at 40°C (±0.1) for 90 min with a 90 min 37°C (±0.1) exposure acting as a control. A significant increase in Hsp70 was observed at 5 am (p < 0.001) and 1 pm (p = 0.028) at 40°C when compared to 37°C but not at 9 pm (p = 0.19). A significant increase was also observed from the basal levels of Hsp70, measured on freshly isolated monocytes and the levels detected after heat shock at 40°C at 5 am (p < 0.001) and 1 pm (p = 0.001), which was not observed at 9 pm (p = 0.15). Furthermore, a significant correlation was observed in the heat shock response at 40°C and that obtained at 37°C (p < 0.001). In conclusion, the heat shock response in monocytes is directly proportional to the amount of Hsp70 present in the cells and the stress response may be much higher at different times of the day.</description><identifier>ISSN: 0939-4451</identifier><identifier>EISSN: 1438-2199</identifier><identifier>DOI: 10.1007/s00726-009-0354-4</identifier><identifier>PMID: 19779802</identifier><language>eng</language><publisher>Vienna: Vienna : Springer Vienna</publisher><subject>Adolescent ; Adult ; Amino acids ; Analytical Chemistry ; Biochemical Engineering ; Biochemistry ; Biomedical and Life Sciences ; Cellular biology ; Correlation ; Density ; diurnal variation ; Flow Cytometry ; Heat shock ; Heat shock proteins ; heat stress ; Hsp70 ; HSP70 Heat-Shock Proteins - metabolism ; Human ; Humans ; In Vitro Techniques ; In vitro testing ; Life Sciences ; Male ; Males ; monocytes ; Monocytes - metabolism ; Neurobiology ; Original Article ; Proteins ; Proteomics ; stress response ; Young Adult</subject><ispartof>Amino acids, 2010-05, Vol.38 (5), p.1423-1428</ispartof><rights>Springer-Verlag 2009</rights><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-9a106747e180a0a025192a8d3e903aac5df6bd902902c23f4d580a37728860883</citedby><cites>FETCH-LOGICAL-c428t-9a106747e180a0a025192a8d3e903aac5df6bd902902c23f4d580a37728860883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00726-009-0354-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00726-009-0354-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19779802$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vince, Rebecca V</creatorcontrib><creatorcontrib>Oliver, Katherine</creatorcontrib><creatorcontrib>Midgley, Adrian W</creatorcontrib><creatorcontrib>McNaughton, Lars R</creatorcontrib><creatorcontrib>Madden, Leigh A</creatorcontrib><title>In vitro heat shock of human monocytes results in a proportional increase of inducible Hsp70 expression according to the basal content</title><title>Amino acids</title><addtitle>Amino Acids</addtitle><addtitle>Amino Acids</addtitle><description>Heat shock proteins play an important role as molecular chaperones of the cell. Inducible heat shock protein 70 is rapidly synthesised in response to numerous stressors and monocytes are sensitive to changes in core temperature resulting in a circadian variation of Hsp70 expression. Monocytes were isolated via density centrifugation from nine healthy male volunteers at 5 am, 1 pm and 9 pm, representing the nadir (5 am), peak (9 pm) and intermediate (1 pm) of Hsp70 expression in the 24-h cycle. Analysis of freshly isolated monocytes for Hsp70 expression confirmed Hsp70 levels at the three selected time points. Monocytes were subjected to in vitro heat shock at 40°C (±0.1) for 90 min with a 90 min 37°C (±0.1) exposure acting as a control. A significant increase in Hsp70 was observed at 5 am (p < 0.001) and 1 pm (p = 0.028) at 40°C when compared to 37°C but not at 9 pm (p = 0.19). A significant increase was also observed from the basal levels of Hsp70, measured on freshly isolated monocytes and the levels detected after heat shock at 40°C at 5 am (p < 0.001) and 1 pm (p = 0.001), which was not observed at 9 pm (p = 0.15). Furthermore, a significant correlation was observed in the heat shock response at 40°C and that obtained at 37°C (p < 0.001). In conclusion, the heat shock response in monocytes is directly proportional to the amount of Hsp70 present in the cells and the stress response may be much higher at different times of the day.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Amino acids</subject><subject>Analytical Chemistry</subject><subject>Biochemical Engineering</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Cellular biology</subject><subject>Correlation</subject><subject>Density</subject><subject>diurnal variation</subject><subject>Flow Cytometry</subject><subject>Heat shock</subject><subject>Heat shock proteins</subject><subject>heat stress</subject><subject>Hsp70</subject><subject>HSP70 Heat-Shock Proteins - metabolism</subject><subject>Human</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>In vitro testing</subject><subject>Life Sciences</subject><subject>Male</subject><subject>Males</subject><subject>monocytes</subject><subject>Monocytes - metabolism</subject><subject>Neurobiology</subject><subject>Original Article</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>stress response</subject><subject>Young Adult</subject><issn>0939-4451</issn><issn>1438-2199</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kcGK1TAUhoMoznX0AdxowIVuqidJ2yTLYVBnYMCFzjrkpum9Hduk5qTivIDPbUovKIKSkAPJ9__hnJ-Q5wzeMgD5DsvB2wpAVyCauqofkB2rhao40_oh2YEWuqrrhp2RJ4h3AIwr1j4mZ0xLqRXwHfl5Hej3IadIj95misfovtLY0-My2UCnGKK7zx5p8riMGekQqKVzinNMeYjBjuXGJW_Rr6ohdIsb9qOnVzhLoP7HXIRYQGqdi6kbwoHmSPPR073FonYxZB_yU_KotyP6Z6d6Tm4_vP9yeVXdfPp4fXlxU7maq1xpy6CVtfRMgS2LN0xzqzrhNQhrXdP17b7TwMt2XPR11xRQSMmVakEpcU5eb76lhW-Lx2ymAZ0fRxt8XNBIITRvpIBCvvkvyYDpVnJgoqCv_kLv4pLKbFZKN21bRr1SbKNciojJ92ZOw2TTfYHMGqfZ4jQlTrPGaeqieXFyXvaT734rTvkVgG8Alqdw8OnPr__t-nIT9TYae0gDmtvPayfAVCFaIX4BGVuzIg</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>Vince, Rebecca V</creator><creator>Oliver, Katherine</creator><creator>Midgley, Adrian W</creator><creator>McNaughton, Lars R</creator><creator>Madden, Leigh A</creator><general>Vienna : Springer Vienna</general><general>Springer Vienna</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PDBOC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20100501</creationdate><title>In vitro heat shock of human monocytes results in a proportional increase of inducible Hsp70 expression according to the basal content</title><author>Vince, Rebecca V ; Oliver, Katherine ; Midgley, Adrian W ; McNaughton, Lars R ; Madden, Leigh A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-9a106747e180a0a025192a8d3e903aac5df6bd902902c23f4d580a37728860883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Amino acids</topic><topic>Analytical Chemistry</topic><topic>Biochemical Engineering</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Cellular biology</topic><topic>Correlation</topic><topic>Density</topic><topic>diurnal variation</topic><topic>Flow Cytometry</topic><topic>Heat shock</topic><topic>Heat shock proteins</topic><topic>heat stress</topic><topic>Hsp70</topic><topic>HSP70 Heat-Shock Proteins - 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Academic</collection><jtitle>Amino acids</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vince, Rebecca V</au><au>Oliver, Katherine</au><au>Midgley, Adrian W</au><au>McNaughton, Lars R</au><au>Madden, Leigh A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro heat shock of human monocytes results in a proportional increase of inducible Hsp70 expression according to the basal content</atitle><jtitle>Amino acids</jtitle><stitle>Amino Acids</stitle><addtitle>Amino Acids</addtitle><date>2010-05-01</date><risdate>2010</risdate><volume>38</volume><issue>5</issue><spage>1423</spage><epage>1428</epage><pages>1423-1428</pages><issn>0939-4451</issn><eissn>1438-2199</eissn><abstract>Heat shock proteins play an important role as molecular chaperones of the cell. Inducible heat shock protein 70 is rapidly synthesised in response to numerous stressors and monocytes are sensitive to changes in core temperature resulting in a circadian variation of Hsp70 expression. Monocytes were isolated via density centrifugation from nine healthy male volunteers at 5 am, 1 pm and 9 pm, representing the nadir (5 am), peak (9 pm) and intermediate (1 pm) of Hsp70 expression in the 24-h cycle. Analysis of freshly isolated monocytes for Hsp70 expression confirmed Hsp70 levels at the three selected time points. Monocytes were subjected to in vitro heat shock at 40°C (±0.1) for 90 min with a 90 min 37°C (±0.1) exposure acting as a control. A significant increase in Hsp70 was observed at 5 am (p < 0.001) and 1 pm (p = 0.028) at 40°C when compared to 37°C but not at 9 pm (p = 0.19). A significant increase was also observed from the basal levels of Hsp70, measured on freshly isolated monocytes and the levels detected after heat shock at 40°C at 5 am (p < 0.001) and 1 pm (p = 0.001), which was not observed at 9 pm (p = 0.15). Furthermore, a significant correlation was observed in the heat shock response at 40°C and that obtained at 37°C (p < 0.001). In conclusion, the heat shock response in monocytes is directly proportional to the amount of Hsp70 present in the cells and the stress response may be much higher at different times of the day.</abstract><cop>Vienna</cop><pub>Vienna : Springer Vienna</pub><pmid>19779802</pmid><doi>10.1007/s00726-009-0354-4</doi><tpages>6</tpages></addata></record> |
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| ispartof | Amino acids, 2010-05, Vol.38 (5), p.1423-1428 |
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| subjects | Adolescent Adult Amino acids Analytical Chemistry Biochemical Engineering Biochemistry Biomedical and Life Sciences Cellular biology Correlation Density diurnal variation Flow Cytometry Heat shock Heat shock proteins heat stress Hsp70 HSP70 Heat-Shock Proteins - metabolism Human Humans In Vitro Techniques In vitro testing Life Sciences Male Males monocytes Monocytes - metabolism Neurobiology Original Article Proteins Proteomics stress response Young Adult |
| title | In vitro heat shock of human monocytes results in a proportional increase of inducible Hsp70 expression according to the basal content |
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