WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana
Boron (B) is one of the essential nutrients for plant growth and reproduction. Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency-inducible genes, T-DNA insertion mutants of 10 B deficiency-indu...
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| Veröffentlicht in: | Physiologia plantarum 2010-05, Vol.139 (1), p.80-92 |
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| creator | Kasajima, Ichiro Ide, Yoko Yokota Hirai, Masami Fujiwara, Toru |
| description | Boron (B) is one of the essential nutrients for plant growth and reproduction. Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency-inducible genes, T-DNA insertion mutants of 10 B deficiency-induced genes were obtained, and their growth properties in response to B conditions were examined. Among the lines examined, mutants of the transcription factor WRKY6 showed growth defect compared with the wild-type under B deficiency, but not under normal conditions. This growth defect was commonly observed among three independently isolated wrky6 mutants. There was no significant difference in B concentration between wrky6-3 and the wild-type. Promoter activity of WRKY6 was induced around the root tip under B deficiency. These results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions. Transcriptome analysis around the root tip identified WRKY6-regulated genes under B deficiency. Our findings represent the first identification of a transcription factor involved in the response to B deficiency. |
| doi_str_mv | 10.1111/j.1399-3054.2010.01349.x |
| format | Article |
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Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency-inducible genes, T-DNA insertion mutants of 10 B deficiency-induced genes were obtained, and their growth properties in response to B conditions were examined. Among the lines examined, mutants of the transcription factor WRKY6 showed growth defect compared with the wild-type under B deficiency, but not under normal conditions. This growth defect was commonly observed among three independently isolated wrky6 mutants. There was no significant difference in B concentration between wrky6-3 and the wild-type. Promoter activity of WRKY6 was induced around the root tip under B deficiency. These results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions. Transcriptome analysis around the root tip identified WRKY6-regulated genes under B deficiency. Our findings represent the first identification of a transcription factor involved in the response to B deficiency.</description><identifier>ISSN: 0031-9317</identifier><identifier>EISSN: 1399-3054</identifier><identifier>DOI: 10.1111/j.1399-3054.2010.01349.x</identifier><identifier>PMID: 20059736</identifier><identifier>CODEN: PHPLAI</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - physiology ; Biological and medical sciences ; Boron - deficiency ; Fundamental and applied biological sciences. Psychology ; Gene Expression Regulation, Plant - genetics ; Gene Expression Regulation, Plant - physiology ; Mutagenesis, Insertional ; Oligonucleotide Array Sequence Analysis ; Plant physiology and development ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - metabolism ; Polymerase Chain Reaction ; Transcription Factors - genetics ; Transcription Factors - physiology</subject><ispartof>Physiologia plantarum, 2010-05, Vol.139 (1), p.80-92</ispartof><rights>Copyright © Physiologia Plantarum 2010</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4609-a0b3d4bfefb9d4524815b86842d9545321e1ee57da68e2af0a19b4a872e11f883</citedby><cites>FETCH-LOGICAL-c4609-a0b3d4bfefb9d4524815b86842d9545321e1ee57da68e2af0a19b4a872e11f883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1399-3054.2010.01349.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1399-3054.2010.01349.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22607005$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20059736$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kasajima, Ichiro</creatorcontrib><creatorcontrib>Ide, Yoko</creatorcontrib><creatorcontrib>Yokota Hirai, Masami</creatorcontrib><creatorcontrib>Fujiwara, Toru</creatorcontrib><title>WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana</title><title>Physiologia plantarum</title><addtitle>Physiol Plant</addtitle><description>Boron (B) is one of the essential nutrients for plant growth and reproduction. Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency-inducible genes, T-DNA insertion mutants of 10 B deficiency-induced genes were obtained, and their growth properties in response to B conditions were examined. Among the lines examined, mutants of the transcription factor WRKY6 showed growth defect compared with the wild-type under B deficiency, but not under normal conditions. This growth defect was commonly observed among three independently isolated wrky6 mutants. There was no significant difference in B concentration between wrky6-3 and the wild-type. Promoter activity of WRKY6 was induced around the root tip under B deficiency. These results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions. Transcriptome analysis around the root tip identified WRKY6-regulated genes under B deficiency. Our findings represent the first identification of a transcription factor involved in the response to B deficiency.</description><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - physiology</subject><subject>Biological and medical sciences</subject><subject>Boron - deficiency</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation, Plant - genetics</subject><subject>Gene Expression Regulation, Plant - physiology</subject><subject>Mutagenesis, Insertional</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Plant physiology and development</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - metabolism</subject><subject>Polymerase Chain Reaction</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><issn>0031-9317</issn><issn>1399-3054</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM9v0zAUxy0EYt3GvwC5IE4p_hnbBw7bBAOtG9XGNHGynOSFuaRxsdOt_e9xSNdd8cVP9ufz3tMXoYzgKUnn42JKmNY5w4JPKU6vmDCup5sXaLL_eIkmGDOSa0bkATqMcYExKQpCX6MDirHQkhUTdHl3ffGzyFzMXPfg2weoU5H195AFiCvfRch6n5U--C6roXGVg67aDsxJsKWr_Somt7-3rbOdPUavGttGeLO7j9Dtl88_zr7ms-_n385OZnnFC6xzi0tW87KBptQ1F5QrIkpVKE5rLbhglAABELK2hQJqG2yJLrlVkgIhjVLsCH0Y-66C_7OG2JulixW0re3Ar6ORjGnKlBKJVCNZBR9jgMasglvasDUEmyFLszBDZGaIzAxZmn9Zmk1S3-6GrMsl1HvxKbwEvN8BNla2bYLtKhefOVpgmdjEfRq5R9fC9r8XMPP5bKiSn4--iz1s9r4Nv00hmRTm7urczE8FvZ5fXZjLxL8b-cZ6Y3-FtNPtTerMMFGUS4LZX4EFppk</recordid><startdate>201005</startdate><enddate>201005</enddate><creator>Kasajima, Ichiro</creator><creator>Ide, Yoko</creator><creator>Yokota Hirai, Masami</creator><creator>Fujiwara, Toru</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201005</creationdate><title>WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana</title><author>Kasajima, Ichiro ; Ide, Yoko ; Yokota Hirai, Masami ; Fujiwara, Toru</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4609-a0b3d4bfefb9d4524815b86842d9545321e1ee57da68e2af0a19b4a872e11f883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - physiology</topic><topic>Biological and medical sciences</topic><topic>Boron - deficiency</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation, Plant - genetics</topic><topic>Gene Expression Regulation, Plant - physiology</topic><topic>Mutagenesis, Insertional</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Plant physiology and development</topic><topic>Plants, Genetically Modified - genetics</topic><topic>Plants, Genetically Modified - metabolism</topic><topic>Polymerase Chain Reaction</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kasajima, Ichiro</creatorcontrib><creatorcontrib>Ide, Yoko</creatorcontrib><creatorcontrib>Yokota Hirai, Masami</creatorcontrib><creatorcontrib>Fujiwara, Toru</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Physiologia plantarum</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kasajima, Ichiro</au><au>Ide, Yoko</au><au>Yokota Hirai, Masami</au><au>Fujiwara, Toru</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana</atitle><jtitle>Physiologia plantarum</jtitle><addtitle>Physiol Plant</addtitle><date>2010-05</date><risdate>2010</risdate><volume>139</volume><issue>1</issue><spage>80</spage><epage>92</epage><pages>80-92</pages><issn>0031-9317</issn><eissn>1399-3054</eissn><coden>PHPLAI</coden><abstract>Boron (B) is one of the essential nutrients for plant growth and reproduction. Transcriptome analyses have identified genes regulated by B deficiency, but their function mostly remains elusive. To identify the functions of B deficiency-inducible genes, T-DNA insertion mutants of 10 B deficiency-induced genes were obtained, and their growth properties in response to B conditions were examined. Among the lines examined, mutants of the transcription factor WRKY6 showed growth defect compared with the wild-type under B deficiency, but not under normal conditions. This growth defect was commonly observed among three independently isolated wrky6 mutants. There was no significant difference in B concentration between wrky6-3 and the wild-type. Promoter activity of WRKY6 was induced around the root tip under B deficiency. These results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions. Transcriptome analysis around the root tip identified WRKY6-regulated genes under B deficiency. Our findings represent the first identification of a transcription factor involved in the response to B deficiency.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>20059736</pmid><doi>10.1111/j.1399-3054.2010.01349.x</doi><tpages>13</tpages></addata></record> |
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| subjects | Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis Proteins - genetics Arabidopsis Proteins - physiology Biological and medical sciences Boron - deficiency Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Plant - genetics Gene Expression Regulation, Plant - physiology Mutagenesis, Insertional Oligonucleotide Array Sequence Analysis Plant physiology and development Plants, Genetically Modified - genetics Plants, Genetically Modified - metabolism Polymerase Chain Reaction Transcription Factors - genetics Transcription Factors - physiology |
| title | WRKY6 is involved in the response to boron deficiency in Arabidopsis thaliana |
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