Morphological and cytoskeletal changes in epithelial cells occur immediately upon interaction with Salmonella typhimurium grown under low‐oxygen conditions
Summary Salmonella typhimurium grown under oxygen‐limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp‐2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entr...
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| Veröffentlicht in: | Molecular microbiology 1992-11, Vol.6 (21), p.3077-3087 |
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| container_title | Molecular microbiology |
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| creator | Francis, C. L. Starnbach, M. N. Falkow, S. |
| description | Summary
Salmonella typhimurium grown under oxygen‐limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp‐2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entry began within 1 min of productive adherence. Polarized Caco‐2 cell morphology was affected 40 s after infection with low‐oxygen‐grown S. typhimurium. Stationary‐phase S. typhimurium did not elicit these phenomena within this time‐period even when adherence was enhanced with the afimbrial adhesin, AFA‐I. Thus, environmental cues regulate S. typhimurium invasion factors, allowing for immediate entry into host cells. Additionally, actin filament rearrangement and morphological changes in the eukaryotic host cell are essential for entry and occur within minutes of infection. |
| doi_str_mv | 10.1111/j.1365-2958.1992.tb01765.x |
| format | Article |
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Salmonella typhimurium grown under oxygen‐limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp‐2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entry began within 1 min of productive adherence. Polarized Caco‐2 cell morphology was affected 40 s after infection with low‐oxygen‐grown S. typhimurium. Stationary‐phase S. typhimurium did not elicit these phenomena within this time‐period even when adherence was enhanced with the afimbrial adhesin, AFA‐I. Thus, environmental cues regulate S. typhimurium invasion factors, allowing for immediate entry into host cells. Additionally, actin filament rearrangement and morphological changes in the eukaryotic host cell are essential for entry and occur within minutes of infection.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.1992.tb01765.x</identifier><identifier>PMID: 1360615</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Actins - metabolism ; Adhesins, Escherichia coli ; Animals ; Bacterial Adhesion - physiology ; Bacterial Outer Membrane Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Cell Line ; Cell Movement - physiology ; Cytoskeleton - metabolism ; Dogs ; Epithelial Cells ; Epithelium - microbiology ; Epithelium - ultrastructure ; Fundamental and applied biological sciences. Psychology ; Humans ; Microbiology ; Microscopy ; Oxygen - physiology ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Salmonella typhimurium ; Salmonella typhimurium - growth & development ; Salmonella typhimurium - pathogenicity ; Tumor Cells, Cultured</subject><ispartof>Molecular microbiology, 1992-11, Vol.6 (21), p.3077-3087</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4307-84f870e0470ac6aaa3710ef33b6f94954aaac1d14ddafeb25c5d518e1b325a573</citedby><cites>FETCH-LOGICAL-c4307-84f870e0470ac6aaa3710ef33b6f94954aaac1d14ddafeb25c5d518e1b325a573</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.1992.tb01765.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.1992.tb01765.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4460394$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1360615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Francis, C. L.</creatorcontrib><creatorcontrib>Starnbach, M. N.</creatorcontrib><creatorcontrib>Falkow, S.</creatorcontrib><title>Morphological and cytoskeletal changes in epithelial cells occur immediately upon interaction with Salmonella typhimurium grown under low‐oxygen conditions</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Salmonella typhimurium grown under oxygen‐limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp‐2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entry began within 1 min of productive adherence. Polarized Caco‐2 cell morphology was affected 40 s after infection with low‐oxygen‐grown S. typhimurium. Stationary‐phase S. typhimurium did not elicit these phenomena within this time‐period even when adherence was enhanced with the afimbrial adhesin, AFA‐I. Thus, environmental cues regulate S. typhimurium invasion factors, allowing for immediate entry into host cells. Additionally, actin filament rearrangement and morphological changes in the eukaryotic host cell are essential for entry and occur within minutes of infection.</description><subject>Actins - metabolism</subject><subject>Adhesins, Escherichia coli</subject><subject>Animals</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacterial Outer Membrane Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Cell Movement - physiology</subject><subject>Cytoskeleton - metabolism</subject><subject>Dogs</subject><subject>Epithelial Cells</subject><subject>Epithelium - microbiology</subject><subject>Epithelium - ultrastructure</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>Oxygen - physiology</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Salmonella typhimurium</subject><subject>Salmonella typhimurium - growth & development</subject><subject>Salmonella typhimurium - pathogenicity</subject><subject>Tumor Cells, Cultured</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkcuO0zAUhi0EGjoDj4BkIcQuwY7tXNggNGJgpKlYABI7y3VOWhfHDnaiNjsegRfg5XgSHLUalghvbJ3z_b59CD2nJKdpvNrnlJUiKxpR57RpinzcEFqVIj8-QKv71kO0Io0gGauLr4_RZYx7QigjJbtAFwkiJRUr9Gvtw7Dz1m-NVhYr12I9jz5-AwtjKuidcluI2DgMgxl3YM1SBWsj9lpPAZu-h9aoEeyMp8G7hI4QlB5NWh9SBH9StvcuRRQe52Fn-imYqcfb4A8OT66FgK0__P7x0x_nLTisvWvNEo9P0KNO2QhPz_MV-nLz7vP1h-zu4_vb67d3meaMVFnNu7oiQHhFlC6VUqyiBDrGNmXX8EbwVNK0pbxtVQebQmjRCloD3bBCKFGxK_TytO8Q_PcJ4ih7E5dHKgd-irJirCkK1vwTpCWrKWciga9PoA4-xgCdHILpVZglJXKRKPdyMSUXU3KRKM8S5TGFn51PmTbpc_9GT9ZS_8W5r2Ky1gXltIn3GOclYQ1P2JsTdjAW5v-4gFyvb9O_VuwPDPG_8Q</recordid><startdate>199211</startdate><enddate>199211</enddate><creator>Francis, C. L.</creator><creator>Starnbach, M. N.</creator><creator>Falkow, S.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>199211</creationdate><title>Morphological and cytoskeletal changes in epithelial cells occur immediately upon interaction with Salmonella typhimurium grown under low‐oxygen conditions</title><author>Francis, C. L. ; Starnbach, M. N. ; Falkow, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4307-84f870e0470ac6aaa3710ef33b6f94954aaac1d14ddafeb25c5d518e1b325a573</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Actins - metabolism</topic><topic>Adhesins, Escherichia coli</topic><topic>Animals</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacterial Outer Membrane Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Cell Movement - physiology</topic><topic>Cytoskeleton - metabolism</topic><topic>Dogs</topic><topic>Epithelial Cells</topic><topic>Epithelium - microbiology</topic><topic>Epithelium - ultrastructure</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Microbiology</topic><topic>Microscopy</topic><topic>Oxygen - physiology</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Salmonella typhimurium</topic><topic>Salmonella typhimurium - growth & development</topic><topic>Salmonella typhimurium - pathogenicity</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Francis, C. L.</creatorcontrib><creatorcontrib>Starnbach, M. N.</creatorcontrib><creatorcontrib>Falkow, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Francis, C. L.</au><au>Starnbach, M. N.</au><au>Falkow, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological and cytoskeletal changes in epithelial cells occur immediately upon interaction with Salmonella typhimurium grown under low‐oxygen conditions</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>1992-11</date><risdate>1992</risdate><volume>6</volume><issue>21</issue><spage>3077</spage><epage>3087</epage><pages>3077-3087</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Salmonella typhimurium grown under oxygen‐limiting conditions were found to enter into, elicit actin filament rearrangement in, and effect morphological changes upon HEp‐2 cells within 15 min after infection. Video microscopy revealed that host cell morphological changes associated with entry began within 1 min of productive adherence. Polarized Caco‐2 cell morphology was affected 40 s after infection with low‐oxygen‐grown S. typhimurium. Stationary‐phase S. typhimurium did not elicit these phenomena within this time‐period even when adherence was enhanced with the afimbrial adhesin, AFA‐I. Thus, environmental cues regulate S. typhimurium invasion factors, allowing for immediate entry into host cells. Additionally, actin filament rearrangement and morphological changes in the eukaryotic host cell are essential for entry and occur within minutes of infection.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1360615</pmid><doi>10.1111/j.1365-2958.1992.tb01765.x</doi><tpages>11</tpages></addata></record> |
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| identifier | ISSN: 0950-382X |
| ispartof | Molecular microbiology, 1992-11, Vol.6 (21), p.3077-3087 |
| issn | 0950-382X 1365-2958 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Actins - metabolism Adhesins, Escherichia coli Animals Bacterial Adhesion - physiology Bacterial Outer Membrane Proteins - metabolism Bacteriology Biological and medical sciences Cell Line Cell Movement - physiology Cytoskeleton - metabolism Dogs Epithelial Cells Epithelium - microbiology Epithelium - ultrastructure Fundamental and applied biological sciences. Psychology Humans Microbiology Microscopy Oxygen - physiology Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Salmonella typhimurium Salmonella typhimurium - growth & development Salmonella typhimurium - pathogenicity Tumor Cells, Cultured |
| title | Morphological and cytoskeletal changes in epithelial cells occur immediately upon interaction with Salmonella typhimurium grown under low‐oxygen conditions |
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