Biosurfactant synthesis by Pseudomonas aeruginosa LBI isolated from a hydrocarbon-contaminated site
Pseudomonas aeruginosa LBI (Industrial Biotechnology Laboratory) was isolated from hydrocarbon-contaminated soil as a potential producer of biosurfactant and evaluated for hydrocarbon biodegradation. The emulsifying power and stability of the product was assessed in the laboratory, simulating water...
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| Veröffentlicht in: | Journal of applied microbiology 2008-11, Vol.105 (5), p.1484-1490 |
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| container_title | Journal of applied microbiology |
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| creator | Pirôllo, M.P.S Mariano, A.P Lovaglio, R.B Costa, S.G.V.A.O Walter, V Hausmann, R Contiero, J |
| description | Pseudomonas aeruginosa LBI (Industrial Biotechnology Laboratory) was isolated from hydrocarbon-contaminated soil as a potential producer of biosurfactant and evaluated for hydrocarbon biodegradation. The emulsifying power and stability of the product was assessed in the laboratory, simulating water contamination with benzene, toluene, kerosene, diesel oil and crude oil at various concentrations. Bacteria were grown at 30°C and shaken at 200 rpm for 168 h, with three repetitions. Surface tension, pH and biosurfactant stability were observed in the cell-free broth after 168 h of incubation. The strain was able to produce biosurfactant and grow in all the carbon sources under study, except benzene and toluene. When cultivated in 30% (w/v) diesel oil, the strain produced the highest quantities (9·9 g l⁻¹) of biosurfactant. The biosurfactant was capable of emulsifying all the hydrocarbons tested. The results from the present study demonstrate that Ps. aeruginosa LBI can grow in diesel oil, kerosene, crude oil and oil sludge and the biosurfactant produced has potential applications in the bioremediation of hydrocarbon-contaminated sites. Pseudomonas aeruginosa LBI or the biosurfactant it produces can be used in the bioremediation of environmental pollution induced by industrial discharge or accidental hydrocarbon spills. |
| doi_str_mv | 10.1111/j.1365-2672.2008.03893.x |
| format | Article |
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The emulsifying power and stability of the product was assessed in the laboratory, simulating water contamination with benzene, toluene, kerosene, diesel oil and crude oil at various concentrations. Bacteria were grown at 30°C and shaken at 200 rpm for 168 h, with three repetitions. Surface tension, pH and biosurfactant stability were observed in the cell-free broth after 168 h of incubation. The strain was able to produce biosurfactant and grow in all the carbon sources under study, except benzene and toluene. When cultivated in 30% (w/v) diesel oil, the strain produced the highest quantities (9·9 g l⁻¹) of biosurfactant. The biosurfactant was capable of emulsifying all the hydrocarbons tested. The results from the present study demonstrate that Ps. aeruginosa LBI can grow in diesel oil, kerosene, crude oil and oil sludge and the biosurfactant produced has potential applications in the bioremediation of hydrocarbon-contaminated sites. Pseudomonas aeruginosa LBI or the biosurfactant it produces can be used in the bioremediation of environmental pollution induced by industrial discharge or accidental hydrocarbon spills.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/j.1365-2672.2008.03893.x</identifier><identifier>PMID: 18795978</identifier><language>eng</language><publisher>Oxford, UK: Oxford, UK : Blackwell Publishing Ltd</publisher><subject>Biodegradation, Environmental ; Biological and medical sciences ; bioremediation ; biosurfactant ; Biotechnology - methods ; Fundamental and applied biological sciences. Psychology ; hydrocarbon ; Hydrocarbons - chemistry ; Hydrogen-Ion Concentration ; Microbiology ; oil sludge ; Petroleum - metabolism ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - growth & development ; Pseudomonas aeruginosa - metabolism ; Pseudomonas aeruginosa LBI ; Soil Microbiology ; Soil Pollutants - chemistry ; Surface Tension ; Surface-Active Agents - chemistry</subject><ispartof>Journal of applied microbiology, 2008-11, Vol.105 (5), p.1484-1490</ispartof><rights>2008 The Authors. Journal compilation © 2008 The Society for Applied Microbiology</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4533-d7a7d175ccd3ab98c21037e45e25f5b48c0cfe3cfba9773570820b0218845cf23</citedby><cites>FETCH-LOGICAL-c4533-d7a7d175ccd3ab98c21037e45e25f5b48c0cfe3cfba9773570820b0218845cf23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2672.2008.03893.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2672.2008.03893.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20781369$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18795978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pirôllo, M.P.S</creatorcontrib><creatorcontrib>Mariano, A.P</creatorcontrib><creatorcontrib>Lovaglio, R.B</creatorcontrib><creatorcontrib>Costa, S.G.V.A.O</creatorcontrib><creatorcontrib>Walter, V</creatorcontrib><creatorcontrib>Hausmann, R</creatorcontrib><creatorcontrib>Contiero, J</creatorcontrib><title>Biosurfactant synthesis by Pseudomonas aeruginosa LBI isolated from a hydrocarbon-contaminated site</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Pseudomonas aeruginosa LBI (Industrial Biotechnology Laboratory) was isolated from hydrocarbon-contaminated soil as a potential producer of biosurfactant and evaluated for hydrocarbon biodegradation. The emulsifying power and stability of the product was assessed in the laboratory, simulating water contamination with benzene, toluene, kerosene, diesel oil and crude oil at various concentrations. Bacteria were grown at 30°C and shaken at 200 rpm for 168 h, with three repetitions. Surface tension, pH and biosurfactant stability were observed in the cell-free broth after 168 h of incubation. The strain was able to produce biosurfactant and grow in all the carbon sources under study, except benzene and toluene. When cultivated in 30% (w/v) diesel oil, the strain produced the highest quantities (9·9 g l⁻¹) of biosurfactant. The biosurfactant was capable of emulsifying all the hydrocarbons tested. The results from the present study demonstrate that Ps. aeruginosa LBI can grow in diesel oil, kerosene, crude oil and oil sludge and the biosurfactant produced has potential applications in the bioremediation of hydrocarbon-contaminated sites. Pseudomonas aeruginosa LBI or the biosurfactant it produces can be used in the bioremediation of environmental pollution induced by industrial discharge or accidental hydrocarbon spills.</description><subject>Biodegradation, Environmental</subject><subject>Biological and medical sciences</subject><subject>bioremediation</subject><subject>biosurfactant</subject><subject>Biotechnology - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hydrocarbon</subject><subject>Hydrocarbons - chemistry</subject><subject>Hydrogen-Ion Concentration</subject><subject>Microbiology</subject><subject>oil sludge</subject><subject>Petroleum - metabolism</subject><subject>Pseudomonas aeruginosa</subject><subject>Pseudomonas aeruginosa - growth & development</subject><subject>Pseudomonas aeruginosa - metabolism</subject><subject>Pseudomonas aeruginosa LBI</subject><subject>Soil Microbiology</subject><subject>Soil Pollutants - chemistry</subject><subject>Surface Tension</subject><subject>Surface-Active Agents - chemistry</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk1v1DAQhiMEoqXwF8AX4JTgj3jtHDi0FR9Fi0CCnq2JY7deJXbxJKL59yS7q3JD-DIjzfPOjN5xURBGK7a8d7uKiY0s-UbxilOqKyp0I6r7R8XpQ-HxPq9LSRU_KZ4h7ihlgsrN0-KEadXIRunTwl6EhFP2YEeII8E5jrcOA5J2Jt_RTV0aUgQk4PJ0E2JCINuLKxIw9TC6jvicBgLkdu5yspDbFEub4ghDiPs6htE9L5546NG9OMaz4vrjh5-Xn8vtt09Xl-fb0tZSiLJToDqmpLWdgLbRljMqlKul49LLttaWWu-E9S00SgmpqOa0pZxpXUvruTgr3h763uX0a3I4miGgdX0P0aUJjRKiYZptmoV880-SNVLXjNIF1AfQ5oSYnTd3OQyQZ8OoWU9hdmZ13KyOm_UUZn8Kc79IXx5nTO3gur_Co_cL8PoIAFrofYZoAz5wnCq9tF6XfX_gfofezf-9gPly_nXNFv2rg95DMnCTlxnXP_j6F5iUVDZa_AFuGK6g</recordid><startdate>200811</startdate><enddate>200811</enddate><creator>Pirôllo, M.P.S</creator><creator>Mariano, A.P</creator><creator>Lovaglio, R.B</creator><creator>Costa, S.G.V.A.O</creator><creator>Walter, V</creator><creator>Hausmann, R</creator><creator>Contiero, J</creator><general>Oxford, UK : Blackwell Publishing Ltd</general><general>Blackwell Publishing Ltd</general><general>Blackwell Science</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TV</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200811</creationdate><title>Biosurfactant synthesis by Pseudomonas aeruginosa LBI isolated from a hydrocarbon-contaminated site</title><author>Pirôllo, M.P.S ; Mariano, A.P ; Lovaglio, R.B ; Costa, S.G.V.A.O ; Walter, V ; Hausmann, R ; Contiero, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4533-d7a7d175ccd3ab98c21037e45e25f5b48c0cfe3cfba9773570820b0218845cf23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Biodegradation, Environmental</topic><topic>Biological and medical sciences</topic><topic>bioremediation</topic><topic>biosurfactant</topic><topic>Biotechnology - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hydrocarbon</topic><topic>Hydrocarbons - chemistry</topic><topic>Hydrogen-Ion Concentration</topic><topic>Microbiology</topic><topic>oil sludge</topic><topic>Petroleum - metabolism</topic><topic>Pseudomonas aeruginosa</topic><topic>Pseudomonas aeruginosa - growth & development</topic><topic>Pseudomonas aeruginosa - metabolism</topic><topic>Pseudomonas aeruginosa LBI</topic><topic>Soil Microbiology</topic><topic>Soil Pollutants - chemistry</topic><topic>Surface Tension</topic><topic>Surface-Active Agents - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pirôllo, M.P.S</creatorcontrib><creatorcontrib>Mariano, A.P</creatorcontrib><creatorcontrib>Lovaglio, R.B</creatorcontrib><creatorcontrib>Costa, S.G.V.A.O</creatorcontrib><creatorcontrib>Walter, V</creatorcontrib><creatorcontrib>Hausmann, R</creatorcontrib><creatorcontrib>Contiero, J</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Pollution Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pirôllo, M.P.S</au><au>Mariano, A.P</au><au>Lovaglio, R.B</au><au>Costa, S.G.V.A.O</au><au>Walter, V</au><au>Hausmann, R</au><au>Contiero, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosurfactant synthesis by Pseudomonas aeruginosa LBI isolated from a hydrocarbon-contaminated site</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2008-11</date><risdate>2008</risdate><volume>105</volume><issue>5</issue><spage>1484</spage><epage>1490</epage><pages>1484-1490</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Pseudomonas aeruginosa LBI (Industrial Biotechnology Laboratory) was isolated from hydrocarbon-contaminated soil as a potential producer of biosurfactant and evaluated for hydrocarbon biodegradation. The emulsifying power and stability of the product was assessed in the laboratory, simulating water contamination with benzene, toluene, kerosene, diesel oil and crude oil at various concentrations. Bacteria were grown at 30°C and shaken at 200 rpm for 168 h, with three repetitions. Surface tension, pH and biosurfactant stability were observed in the cell-free broth after 168 h of incubation. The strain was able to produce biosurfactant and grow in all the carbon sources under study, except benzene and toluene. When cultivated in 30% (w/v) diesel oil, the strain produced the highest quantities (9·9 g l⁻¹) of biosurfactant. The biosurfactant was capable of emulsifying all the hydrocarbons tested. The results from the present study demonstrate that Ps. aeruginosa LBI can grow in diesel oil, kerosene, crude oil and oil sludge and the biosurfactant produced has potential applications in the bioremediation of hydrocarbon-contaminated sites. Pseudomonas aeruginosa LBI or the biosurfactant it produces can be used in the bioremediation of environmental pollution induced by industrial discharge or accidental hydrocarbon spills.</abstract><cop>Oxford, UK</cop><pub>Oxford, UK : Blackwell Publishing Ltd</pub><pmid>18795978</pmid><doi>10.1111/j.1365-2672.2008.03893.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of applied microbiology, 2008-11, Vol.105 (5), p.1484-1490 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biodegradation, Environmental Biological and medical sciences bioremediation biosurfactant Biotechnology - methods Fundamental and applied biological sciences. Psychology hydrocarbon Hydrocarbons - chemistry Hydrogen-Ion Concentration Microbiology oil sludge Petroleum - metabolism Pseudomonas aeruginosa Pseudomonas aeruginosa - growth & development Pseudomonas aeruginosa - metabolism Pseudomonas aeruginosa LBI Soil Microbiology Soil Pollutants - chemistry Surface Tension Surface-Active Agents - chemistry |
| title | Biosurfactant synthesis by Pseudomonas aeruginosa LBI isolated from a hydrocarbon-contaminated site |
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