Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy
Previous studies have shown that protein deficiencies can hamper both the course of experimental schistosomiasis and normal development of adult worms. To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphome...
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| Veröffentlicht in: | Journal of helminthology 2009-03, Vol.83 (1), p.13-21 |
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| creator | Barros, L.A. Neves, R.H. de Moura, E.G. Machado-Silva, J.R. |
| description | Previous studies have shown that protein deficiencies can hamper both the course of experimental schistosomiasis and normal development of adult worms. To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphometric and confocal laser scanning microscopy analysis. Swiss mice were fed protein-deficient diets (8%) and infected subcutaneously with approximately 80 S.mansoni cercariae (BH strain, Brazil). Control mice were fed a standard rodent diet (23% protein). The nutritional status was evaluated by body weight gain and albumin values. Mice were sacrificed 63 days post-infection. Recovered worms were stained with hydrochloric carmine and preserved as whole-mounts for bright-field examination and confocal microscopy. The body weight gain and serum albumin concentrations were significantly lower (P |
| doi_str_mv | 10.1017/S0022149X08067217 |
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To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphometric and confocal laser scanning microscopy analysis. Swiss mice were fed protein-deficient diets (8%) and infected subcutaneously with approximately 80 S.mansoni cercariae (BH strain, Brazil). Control mice were fed a standard rodent diet (23% protein). The nutritional status was evaluated by body weight gain and albumin values. Mice were sacrificed 63 days post-infection. Recovered worms were stained with hydrochloric carmine and preserved as whole-mounts for bright-field examination and confocal microscopy. The body weight gain and serum albumin concentrations were significantly lower (P < 0.05) in malnourished mice than in controls. In general, all morphometric values of specimens grown in malnourished mice were lower than those of control mice. Schistosome worms grown in malnourished mice had statistically significant differences (P < 0.05) in the reproductive system and tegument than those grown in mice fed standard diets. In female worms, vitelline glands showed few remaining follicles and ovaries lacked mature oocytes. In male parasites, tubercles were fewer in number on the dorsal surface and testicular lobes presented fewer differentiated germinal cells. In summary, we describe novel data supporting the view that low-protein diets may influence the development of adult worms.</description><identifier>ISSN: 0022-149X</identifier><identifier>EISSN: 1475-2697</identifier><identifier>DOI: 10.1017/S0022149X08067217</identifier><identifier>PMID: 18845028</identifier><identifier>CODEN: JOHLAT</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animal and plant ecology ; Animal, plant and microbial ecology ; Animals ; Autoecology ; Biological and medical sciences ; Body Weight ; Child ; Disease Models, Animal ; Female ; Fundamental and applied biological sciences. Psychology ; Host-Parasite Interactions ; Humans ; Image Processing, Computer-Assisted ; Male ; Mice ; Microscopy, Confocal ; Protein Deficiency - parasitology ; Protozoa. Invertebrata ; Schistosoma mansoni - growth & development ; Schistosoma mansoni - isolation & purification ; Schistosoma mansoni - ultrastructure ; Schistosomiasis mansoni - parasitology ; Serum Albumin - analysis</subject><ispartof>Journal of helminthology, 2009-03, Vol.83 (1), p.13-21</ispartof><rights>Copyright © Cambridge University Press 2009</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c439t-f8db427ea215ecff8516e36e3409fb84800aa8e3287050e809b9811ad6cd7b83</citedby><cites>FETCH-LOGICAL-c439t-f8db427ea215ecff8516e36e3409fb84800aa8e3287050e809b9811ad6cd7b83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0022149X08067217/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21231251$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18845028$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Barros, L.A.</creatorcontrib><creatorcontrib>Neves, R.H.</creatorcontrib><creatorcontrib>de Moura, E.G.</creatorcontrib><creatorcontrib>Machado-Silva, J.R.</creatorcontrib><title>Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy</title><title>Journal of helminthology</title><addtitle>J. Helminthol</addtitle><description>Previous studies have shown that protein deficiencies can hamper both the course of experimental schistosomiasis and normal development of adult worms. To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphometric and confocal laser scanning microscopy analysis. Swiss mice were fed protein-deficient diets (8%) and infected subcutaneously with approximately 80 S.mansoni cercariae (BH strain, Brazil). Control mice were fed a standard rodent diet (23% protein). The nutritional status was evaluated by body weight gain and albumin values. Mice were sacrificed 63 days post-infection. Recovered worms were stained with hydrochloric carmine and preserved as whole-mounts for bright-field examination and confocal microscopy. The body weight gain and serum albumin concentrations were significantly lower (P < 0.05) in malnourished mice than in controls. In general, all morphometric values of specimens grown in malnourished mice were lower than those of control mice. Schistosome worms grown in malnourished mice had statistically significant differences (P < 0.05) in the reproductive system and tegument than those grown in mice fed standard diets. In female worms, vitelline glands showed few remaining follicles and ovaries lacked mature oocytes. In male parasites, tubercles were fewer in number on the dorsal surface and testicular lobes presented fewer differentiated germinal cells. In summary, we describe novel data supporting the view that low-protein diets may influence the development of adult worms.</description><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Animals</subject><subject>Autoecology</subject><subject>Biological and medical sciences</subject><subject>Body Weight</subject><subject>Child</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Host-Parasite Interactions</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted</subject><subject>Male</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>Protein Deficiency - parasitology</subject><subject>Protozoa. Invertebrata</subject><subject>Schistosoma mansoni - growth & development</subject><subject>Schistosoma mansoni - isolation & purification</subject><subject>Schistosoma mansoni - ultrastructure</subject><subject>Schistosomiasis mansoni - parasitology</subject><subject>Serum Albumin - analysis</subject><issn>0022-149X</issn><issn>1475-2697</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><recordid>eNp1kUtr3DAUhUVpaaZpf0A3RRRCVk70sC152Ql5QaCkmUV3QpavJkptaSrZbdxFf3ttxiTQUhBocb57dY4OQu8pOaGEitM7QhijefWVSFIKRsULtKK5KDJWVuIlWs1yNusH6E1KD4QQTlnxGh1QKfOCMLlCv8-tBdMnHCxuw89sF0MPzuPGQY-Dx3fm3qU-pNBp3Gmfgne4C3F3H9qwHfEPlwbdul_Q4HpchA76OGLtG2yCt8HoFrc6QcTJaO-d3-LOmRiSCbvxLXpldZvg3XIfos3F-ebsKrv5fHl99ukmMzmv-szKps6ZAM1oAcZaWdAS-HRyUtla5pIQrSVwJgUpCEhS1ZWkVDelaUQt-SE63q-d4n0fIPWqc8lA22oPYUhKcF5RIcqZ_PgX-RCG6CdvilFe0LzIZ4juoTlGimDVLrpOx1FRouZm1D_NTDMflsVD3UHzPLFUMQFHC6Cnj2pt1N649MQxyuby6MRle27qBR6fdB2_qVJwUajy8lat-fpq_WVzoeaH-WJWd3V0zRaeI_3f7h9KWbZg</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Barros, L.A.</creator><creator>Neves, R.H.</creator><creator>de Moura, E.G.</creator><creator>Machado-Silva, J.R.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QG</scope><scope>7SN</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H95</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L.G</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy</title><author>Barros, L.A. ; Neves, R.H. ; de Moura, E.G. ; Machado-Silva, J.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c439t-f8db427ea215ecff8516e36e3409fb84800aa8e3287050e809b9811ad6cd7b83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Animals</topic><topic>Autoecology</topic><topic>Biological and medical sciences</topic><topic>Body Weight</topic><topic>Child</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Host-Parasite Interactions</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted</topic><topic>Male</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>Protein Deficiency - parasitology</topic><topic>Protozoa. Invertebrata</topic><topic>Schistosoma mansoni - growth & development</topic><topic>Schistosoma mansoni - isolation & purification</topic><topic>Schistosoma mansoni - ultrastructure</topic><topic>Schistosomiasis mansoni - parasitology</topic><topic>Serum Albumin - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barros, L.A.</creatorcontrib><creatorcontrib>Neves, R.H.</creatorcontrib><creatorcontrib>de Moura, E.G.</creatorcontrib><creatorcontrib>Machado-Silva, J.R.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Ecology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of helminthology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barros, L.A.</au><au>Neves, R.H.</au><au>de Moura, E.G.</au><au>Machado-Silva, J.R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy</atitle><jtitle>Journal of helminthology</jtitle><addtitle>J. Helminthol</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>83</volume><issue>1</issue><spage>13</spage><epage>21</epage><pages>13-21</pages><issn>0022-149X</issn><eissn>1475-2697</eissn><coden>JOHLAT</coden><abstract>Previous studies have shown that protein deficiencies can hamper both the course of experimental schistosomiasis and normal development of adult worms. To further investigate this relationship, we compared adult male and female Schistosoma mansoni from malnourished and well-fed mice through morphometric and confocal laser scanning microscopy analysis. Swiss mice were fed protein-deficient diets (8%) and infected subcutaneously with approximately 80 S.mansoni cercariae (BH strain, Brazil). Control mice were fed a standard rodent diet (23% protein). The nutritional status was evaluated by body weight gain and albumin values. Mice were sacrificed 63 days post-infection. Recovered worms were stained with hydrochloric carmine and preserved as whole-mounts for bright-field examination and confocal microscopy. The body weight gain and serum albumin concentrations were significantly lower (P < 0.05) in malnourished mice than in controls. In general, all morphometric values of specimens grown in malnourished mice were lower than those of control mice. Schistosome worms grown in malnourished mice had statistically significant differences (P < 0.05) in the reproductive system and tegument than those grown in mice fed standard diets. In female worms, vitelline glands showed few remaining follicles and ovaries lacked mature oocytes. In male parasites, tubercles were fewer in number on the dorsal surface and testicular lobes presented fewer differentiated germinal cells. In summary, we describe novel data supporting the view that low-protein diets may influence the development of adult worms.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>18845028</pmid><doi>10.1017/S0022149X08067217</doi><tpages>9</tpages></addata></record> |
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| subjects | Animal and plant ecology Animal, plant and microbial ecology Animals Autoecology Biological and medical sciences Body Weight Child Disease Models, Animal Female Fundamental and applied biological sciences. Psychology Host-Parasite Interactions Humans Image Processing, Computer-Assisted Male Mice Microscopy, Confocal Protein Deficiency - parasitology Protozoa. Invertebrata Schistosoma mansoni - growth & development Schistosoma mansoni - isolation & purification Schistosoma mansoni - ultrastructure Schistosomiasis mansoni - parasitology Serum Albumin - analysis |
| title | Effects of low-protein diet on Schistosoma mansoni morphology visualized by morphometry and confocal laser scanning microscopy |
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