Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi
We characterized a gene encoding an YchF-related protein, TcYchF, potentially associated with the protein translation machinery of Trypanosoma cruzi. YchF belongs to the translation factor-related (TRAFAC) class of P-loop NTPases. The coding region of the gene is 1185 bp long and encodes a 44.3 kDa...
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| creator | Gradia, Daniela F. Rau, Karlan Umaki, Adriana C.S. de Souza, Flavia S.P. Probst, Christian M. Correa, Alejandro Holetz, Fabíola B. Avila, Andréa R. Krieger, Marco A. Goldenberg, Samuel Fragoso, Stenio P. |
| description | We characterized a gene encoding an YchF-related protein, TcYchF, potentially associated with the protein translation machinery of
Trypanosoma cruzi. YchF belongs to the translation factor-related (TRAFAC) class of P-loop NTPases. The coding region of the gene is 1185
bp long and encodes a 44.3
kDa protein. BlastX searches showed TcYchF to be very similar (45–86%) to putative GTP-binding proteins from eukaryotes, including some species of trypanosomatids (
Leishmania major and
Trypanosoma brucei). A lower but significant level of similarity (38–43%) was also found between the predicted sequences of TcYchF and bacterial YyaF/YchF GTPases of the Spo0B-associated GTP-binding protein (Obg) family. Some of the most important features of the G domain of this family of GTPases are conserved in TcYchF. However, we found that TcYchF preferentially hydrolyzed ATP rather than GTP. The function of YyaF/YchF is unknown, but other members of the Obg family are known to be associated with ribosomal subunits. Immunoblots of the polysome fraction from sucrose gradients showed that TcYchF was associated with ribosomal subunits and polysomes. Immunoprecipitation assays showed that TcYchF was also associated with the proteasome of
T. cruzi. Furthermore, inactivation of the
T. brucei homolog of TcYchF by RNA interference inhibited the growth of procyclic forms of the parasite. These data suggest that this protein plays an important role in the translation machinery of trypanosomes. |
| doi_str_mv | 10.1016/j.ijpara.2008.05.019 |
| format | Article |
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Trypanosoma cruzi. YchF belongs to the translation factor-related (TRAFAC) class of P-loop NTPases. The coding region of the gene is 1185
bp long and encodes a 44.3
kDa protein. BlastX searches showed TcYchF to be very similar (45–86%) to putative GTP-binding proteins from eukaryotes, including some species of trypanosomatids (
Leishmania major and
Trypanosoma brucei). A lower but significant level of similarity (38–43%) was also found between the predicted sequences of TcYchF and bacterial YyaF/YchF GTPases of the Spo0B-associated GTP-binding protein (Obg) family. Some of the most important features of the G domain of this family of GTPases are conserved in TcYchF. However, we found that TcYchF preferentially hydrolyzed ATP rather than GTP. The function of YyaF/YchF is unknown, but other members of the Obg family are known to be associated with ribosomal subunits. Immunoblots of the polysome fraction from sucrose gradients showed that TcYchF was associated with ribosomal subunits and polysomes. Immunoprecipitation assays showed that TcYchF was also associated with the proteasome of
T. cruzi. Furthermore, inactivation of the
T. brucei homolog of TcYchF by RNA interference inhibited the growth of procyclic forms of the parasite. These data suggest that this protein plays an important role in the translation machinery of trypanosomes.</description><identifier>ISSN: 0020-7519</identifier><identifier>EISSN: 1879-0135</identifier><identifier>DOI: 10.1016/j.ijpara.2008.05.019</identifier><identifier>PMID: 18713637</identifier><identifier>CODEN: IJPYBT</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Adenosine Triphosphatases - genetics ; Adenosine Triphosphatases - metabolism ; Animals ; Base Sequence ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; Genes, Protozoan ; GTP Phosphohydrolases - metabolism ; GTP-Binding Proteins - genetics ; Leishmania major ; Life cycle. Host-agent relationship. Pathogenesis ; Male ; Molecular Sequence Data ; Obg ; P-loop NTPase ; Polysome ; Proteasome ; Protozoa ; Rabbits ; RNA, Protozoan - metabolism ; Translation ; Trypanosoma brucei ; Trypanosoma brucei brucei - genetics ; Trypanosoma cruzi ; Trypanosoma cruzi - genetics ; YchF</subject><ispartof>International journal for parasitology, 2009, Vol.39 (1), p.49-58</ispartof><rights>2008 Australian Society for Parasitology Inc.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-bf2d33d8d921c7b05d72a4818fc5a60cc34ca29606d05a7e0688332ad81b05c83</citedby><cites>FETCH-LOGICAL-c512t-bf2d33d8d921c7b05d72a4818fc5a60cc34ca29606d05a7e0688332ad81b05c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijpara.2008.05.019$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,4023,27922,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21128041$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18713637$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gradia, Daniela F.</creatorcontrib><creatorcontrib>Rau, Karlan</creatorcontrib><creatorcontrib>Umaki, Adriana C.S.</creatorcontrib><creatorcontrib>de Souza, Flavia S.P.</creatorcontrib><creatorcontrib>Probst, Christian M.</creatorcontrib><creatorcontrib>Correa, Alejandro</creatorcontrib><creatorcontrib>Holetz, Fabíola B.</creatorcontrib><creatorcontrib>Avila, Andréa R.</creatorcontrib><creatorcontrib>Krieger, Marco A.</creatorcontrib><creatorcontrib>Goldenberg, Samuel</creatorcontrib><creatorcontrib>Fragoso, Stenio P.</creatorcontrib><title>Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi</title><title>International journal for parasitology</title><addtitle>Int J Parasitol</addtitle><description>We characterized a gene encoding an YchF-related protein, TcYchF, potentially associated with the protein translation machinery of
Trypanosoma cruzi. YchF belongs to the translation factor-related (TRAFAC) class of P-loop NTPases. The coding region of the gene is 1185
bp long and encodes a 44.3
kDa protein. BlastX searches showed TcYchF to be very similar (45–86%) to putative GTP-binding proteins from eukaryotes, including some species of trypanosomatids (
Leishmania major and
Trypanosoma brucei). A lower but significant level of similarity (38–43%) was also found between the predicted sequences of TcYchF and bacterial YyaF/YchF GTPases of the Spo0B-associated GTP-binding protein (Obg) family. Some of the most important features of the G domain of this family of GTPases are conserved in TcYchF. However, we found that TcYchF preferentially hydrolyzed ATP rather than GTP. The function of YyaF/YchF is unknown, but other members of the Obg family are known to be associated with ribosomal subunits. Immunoblots of the polysome fraction from sucrose gradients showed that TcYchF was associated with ribosomal subunits and polysomes. Immunoprecipitation assays showed that TcYchF was also associated with the proteasome of
T. cruzi. Furthermore, inactivation of the
T. brucei homolog of TcYchF by RNA interference inhibited the growth of procyclic forms of the parasite. These data suggest that this protein plays an important role in the translation machinery of trypanosomes.</description><subject>Adenosine Triphosphatases - genetics</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, Protozoan</subject><subject>GTP Phosphohydrolases - metabolism</subject><subject>GTP-Binding Proteins - genetics</subject><subject>Leishmania major</subject><subject>Life cycle. Host-agent relationship. Pathogenesis</subject><subject>Male</subject><subject>Molecular Sequence Data</subject><subject>Obg</subject><subject>P-loop NTPase</subject><subject>Polysome</subject><subject>Proteasome</subject><subject>Protozoa</subject><subject>Rabbits</subject><subject>RNA, Protozoan - metabolism</subject><subject>Translation</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - genetics</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - genetics</subject><subject>YchF</subject><issn>0020-7519</issn><issn>1879-0135</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90UFv0zAUwHELgVgZfAMEvgCnhGc7jp0L0lSxgTQxJLqz9Wo7m0saFzudtH56XKWC206-_N7T09-EvGVQM2Dt500dNjtMWHMAXYOsgXXPyIJp1VXAhHxOFgAcKiVZd0Ze5bwBYFI0zUtyVhATrVAL8mN5X3bYyadwwCnEkcaeIh3jgx_ozfquGsJvTy9WPzF7GkY63Xu6S3GKh4gjXaXHHY4xxy1Sm_aH8Jq86HHI_s3pPSe3l19Xy2_V9c3V9-XFdWUl41O17rkTwmnXcWbVGqRTHBvNdG8ltmCtaCzyroXWgUTlodVaCI5Os4KtFufk07y33PJn7_NktiFbPww4-rjPRgnRsUZ1vMiPT8pST5YsosBmhjbFnJPvzS6FLaZHw8Aci5uNmYsfZ7QBaUrxMvbutH-_3nr3f-iUuIAPJ4DZ4tAnHG3I_xxnjGtoWHHvZ9djNHiXirn9xctPll9TooHjhV9m4UvZh-CTyTb40XoXkreTcTE8fetfzoiopA</recordid><startdate>2009</startdate><enddate>2009</enddate><creator>Gradia, Daniela F.</creator><creator>Rau, Karlan</creator><creator>Umaki, Adriana C.S.</creator><creator>de Souza, Flavia S.P.</creator><creator>Probst, Christian M.</creator><creator>Correa, Alejandro</creator><creator>Holetz, Fabíola B.</creator><creator>Avila, Andréa R.</creator><creator>Krieger, Marco A.</creator><creator>Goldenberg, Samuel</creator><creator>Fragoso, Stenio P.</creator><general>Elsevier Ltd</general><general>[Oxford; New York]: Elsevier Science</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>2009</creationdate><title>Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi</title><author>Gradia, Daniela F. ; Rau, Karlan ; Umaki, Adriana C.S. ; de Souza, Flavia S.P. ; Probst, Christian M. ; Correa, Alejandro ; Holetz, Fabíola B. ; Avila, Andréa R. ; Krieger, Marco A. ; Goldenberg, Samuel ; Fragoso, Stenio P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-bf2d33d8d921c7b05d72a4818fc5a60cc34ca29606d05a7e0688332ad81b05c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenosine Triphosphatases - genetics</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, Protozoan</topic><topic>GTP Phosphohydrolases - metabolism</topic><topic>GTP-Binding Proteins - genetics</topic><topic>Leishmania major</topic><topic>Life cycle. Host-agent relationship. Pathogenesis</topic><topic>Male</topic><topic>Molecular Sequence Data</topic><topic>Obg</topic><topic>P-loop NTPase</topic><topic>Polysome</topic><topic>Proteasome</topic><topic>Protozoa</topic><topic>Rabbits</topic><topic>RNA, Protozoan - metabolism</topic><topic>Translation</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - genetics</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - genetics</topic><topic>YchF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gradia, Daniela F.</creatorcontrib><creatorcontrib>Rau, Karlan</creatorcontrib><creatorcontrib>Umaki, Adriana C.S.</creatorcontrib><creatorcontrib>de Souza, Flavia S.P.</creatorcontrib><creatorcontrib>Probst, Christian M.</creatorcontrib><creatorcontrib>Correa, Alejandro</creatorcontrib><creatorcontrib>Holetz, Fabíola B.</creatorcontrib><creatorcontrib>Avila, Andréa R.</creatorcontrib><creatorcontrib>Krieger, Marco A.</creatorcontrib><creatorcontrib>Goldenberg, Samuel</creatorcontrib><creatorcontrib>Fragoso, Stenio P.</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>International journal for parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gradia, Daniela F.</au><au>Rau, Karlan</au><au>Umaki, Adriana C.S.</au><au>de Souza, Flavia S.P.</au><au>Probst, Christian M.</au><au>Correa, Alejandro</au><au>Holetz, Fabíola B.</au><au>Avila, Andréa R.</au><au>Krieger, Marco A.</au><au>Goldenberg, Samuel</au><au>Fragoso, Stenio P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi</atitle><jtitle>International journal for parasitology</jtitle><addtitle>Int J Parasitol</addtitle><date>2009</date><risdate>2009</risdate><volume>39</volume><issue>1</issue><spage>49</spage><epage>58</epage><pages>49-58</pages><issn>0020-7519</issn><eissn>1879-0135</eissn><coden>IJPYBT</coden><abstract>We characterized a gene encoding an YchF-related protein, TcYchF, potentially associated with the protein translation machinery of
Trypanosoma cruzi. YchF belongs to the translation factor-related (TRAFAC) class of P-loop NTPases. The coding region of the gene is 1185
bp long and encodes a 44.3
kDa protein. BlastX searches showed TcYchF to be very similar (45–86%) to putative GTP-binding proteins from eukaryotes, including some species of trypanosomatids (
Leishmania major and
Trypanosoma brucei). A lower but significant level of similarity (38–43%) was also found between the predicted sequences of TcYchF and bacterial YyaF/YchF GTPases of the Spo0B-associated GTP-binding protein (Obg) family. Some of the most important features of the G domain of this family of GTPases are conserved in TcYchF. However, we found that TcYchF preferentially hydrolyzed ATP rather than GTP. The function of YyaF/YchF is unknown, but other members of the Obg family are known to be associated with ribosomal subunits. Immunoblots of the polysome fraction from sucrose gradients showed that TcYchF was associated with ribosomal subunits and polysomes. Immunoprecipitation assays showed that TcYchF was also associated with the proteasome of
T. cruzi. Furthermore, inactivation of the
T. brucei homolog of TcYchF by RNA interference inhibited the growth of procyclic forms of the parasite. These data suggest that this protein plays an important role in the translation machinery of trypanosomes.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>18713637</pmid><doi>10.1016/j.ijpara.2008.05.019</doi><tpages>10</tpages></addata></record> |
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| ispartof | International journal for parasitology, 2009, Vol.39 (1), p.49-58 |
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| subjects | Adenosine Triphosphatases - genetics Adenosine Triphosphatases - metabolism Animals Base Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology Genes, Protozoan GTP Phosphohydrolases - metabolism GTP-Binding Proteins - genetics Leishmania major Life cycle. Host-agent relationship. Pathogenesis Male Molecular Sequence Data Obg P-loop NTPase Polysome Proteasome Protozoa Rabbits RNA, Protozoan - metabolism Translation Trypanosoma brucei Trypanosoma brucei brucei - genetics Trypanosoma cruzi Trypanosoma cruzi - genetics YchF |
| title | Characterization of a novel Obg-like ATPase in the protozoan Trypanosoma cruzi |
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