Microwave-assisted extraction followed by CE for determination of catechin and epicatechin in green tea
In this work, for the first time, microwave-assisted extraction (MAE) followed by CE was developed for the fast analysis of catechin and epicatechin in green tea. In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. T...
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| Veröffentlicht in: | Journal of separation science 2010-04, Vol.33 (8), p.1079-1084 |
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| creator | Li, Zhongbo Huang, Danni Tang, Zhuxin Deng, Chunhui |
| description | In this work, for the first time, microwave-assisted extraction (MAE) followed by CE was developed for the fast analysis of catechin and epicatechin in green tea. In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. The MAE conditions and the method's validation were studied. It is found that the extraction time of 1 min with 400 W microwave irradiation is enough to completely extract catechin and epicatechin in green tea sample, whereas the conventional ultrasonic extraction (USE) technique needs long extraction time of 60 min. The method validations were also studied in this work. The calibration curve shows good linearity in 0.01-3 mg/mL for catechin (R²=0.993), and 0.005-3 mg/mL for epicatechin (R²=0.996), respectively. The RSD values for catechin and epicatechin are 0.65 and 2.58%, respectively. This shows that the proposed method has good reproducibility. The proposed method has good recoveries, which are 118% for catechin and 120% for epicatechin. The proposed method was successfully applied to determination of the catechin and epicatechin in different green tea samples. The experiment results have demonstrated that the MAE following CE is a simple, fast and reliable method for the determination of catechin and epicatechin in green tea. |
| doi_str_mv | 10.1002/jssc.200900647 |
| format | Article |
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In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. The MAE conditions and the method's validation were studied. It is found that the extraction time of 1 min with 400 W microwave irradiation is enough to completely extract catechin and epicatechin in green tea sample, whereas the conventional ultrasonic extraction (USE) technique needs long extraction time of 60 min. The method validations were also studied in this work. The calibration curve shows good linearity in 0.01-3 mg/mL for catechin (R²=0.993), and 0.005-3 mg/mL for epicatechin (R²=0.996), respectively. The RSD values for catechin and epicatechin are 0.65 and 2.58%, respectively. This shows that the proposed method has good reproducibility. The proposed method has good recoveries, which are 118% for catechin and 120% for epicatechin. The proposed method was successfully applied to determination of the catechin and epicatechin in different green tea samples. The experiment results have demonstrated that the MAE following CE is a simple, fast and reliable method for the determination of catechin and epicatechin in green tea.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.200900647</identifier><identifier>PMID: 20175087</identifier><language>eng</language><publisher>Weinheim: Wiley-VCH Verlag</publisher><subject>Biological and medical sciences ; Calibration ; Catechin ; Catechin - analysis ; Catechins ; Coffee, tea and other stimulative beverage industries ; Electrophoresis, Capillary - instrumentation ; Epicatechin ; Extraction ; Food industries ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Green tea ; Linearity ; Medical sciences ; Microwave-assisted extraction ; Microwaves ; Molecular Conformation ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Reproducibility ; Reproducibility of Results ; Separation ; Stereoisomerism ; Tea - chemistry</subject><ispartof>Journal of separation science, 2010-04, Vol.33 (8), p.1079-1084</ispartof><rights>Copyright © 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5357-db06804b0832db8a36cc8497f44b8118c949e88c6603e5fff3c522afbcbf36a83</citedby><cites>FETCH-LOGICAL-c5357-db06804b0832db8a36cc8497f44b8118c949e88c6603e5fff3c522afbcbf36a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjssc.200900647$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.200900647$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22629692$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20175087$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Zhongbo</creatorcontrib><creatorcontrib>Huang, Danni</creatorcontrib><creatorcontrib>Tang, Zhuxin</creatorcontrib><creatorcontrib>Deng, Chunhui</creatorcontrib><title>Microwave-assisted extraction followed by CE for determination of catechin and epicatechin in green tea</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>In this work, for the first time, microwave-assisted extraction (MAE) followed by CE was developed for the fast analysis of catechin and epicatechin in green tea. In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. The MAE conditions and the method's validation were studied. It is found that the extraction time of 1 min with 400 W microwave irradiation is enough to completely extract catechin and epicatechin in green tea sample, whereas the conventional ultrasonic extraction (USE) technique needs long extraction time of 60 min. The method validations were also studied in this work. The calibration curve shows good linearity in 0.01-3 mg/mL for catechin (R²=0.993), and 0.005-3 mg/mL for epicatechin (R²=0.996), respectively. The RSD values for catechin and epicatechin are 0.65 and 2.58%, respectively. This shows that the proposed method has good reproducibility. The proposed method has good recoveries, which are 118% for catechin and 120% for epicatechin. The proposed method was successfully applied to determination of the catechin and epicatechin in different green tea samples. The experiment results have demonstrated that the MAE following CE is a simple, fast and reliable method for the determination of catechin and epicatechin in green tea.</description><subject>Biological and medical sciences</subject><subject>Calibration</subject><subject>Catechin</subject><subject>Catechin - analysis</subject><subject>Catechins</subject><subject>Coffee, tea and other stimulative beverage industries</subject><subject>Electrophoresis, Capillary - instrumentation</subject><subject>Epicatechin</subject><subject>Extraction</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Green tea</subject><subject>Linearity</subject><subject>Medical sciences</subject><subject>Microwave-assisted extraction</subject><subject>Microwaves</subject><subject>Molecular Conformation</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Reproducibility</subject><subject>Reproducibility of Results</subject><subject>Separation</subject><subject>Stereoisomerism</subject><subject>Tea - chemistry</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EoqVw5Qi5ILhk8Vf8cUSr0tKWIrFUcLMmjr24ZJPFzrLdf4-XLIETSJY8Yz3vzHhehJ4SPCMY09e3KdkZxVhjLLi8h46JIFWpGeH3pxiLI_QopVuMiVQaP0RHNEcVVvIYLd8HG_st_HAlpBTS4JrC3Q0R7BD6rvB92_bb_FbvivlpTmPRuMHFVejgF9D7wsLg7NfQFdBl7TpMeT7L6FxXDA4eowce2uSeHO4TdPP29NP8vLz6cPZu_uaqtBWrZNnUWCjMa6wYbWoFTFiruJae81oRoqzm2illhcDMVd57ZitKwde29kyAYifo5Vh3HfvvG5cGswrJuraFzvWbZCRjmnCuqky--idJqBCSSypkRmcjmleVUnTerGNYQdwZgs3eBrO3wUw2ZMGzQ-1NvXLNhP_eewZeHABIFlofobMh_eGooFpomjk9ctvQut1_2pqLxWL-9xDlqN27ejdpIX4z-U-yMp-vz8zlJcXXF-cfzZfMPx95D72BZczz3CzyxAwTxQThhP0Eu426EQ</recordid><startdate>201004</startdate><enddate>201004</enddate><creator>Li, Zhongbo</creator><creator>Huang, Danni</creator><creator>Tang, Zhuxin</creator><creator>Deng, Chunhui</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>201004</creationdate><title>Microwave-assisted extraction followed by CE for determination of catechin and epicatechin in green tea</title><author>Li, Zhongbo ; Huang, Danni ; Tang, Zhuxin ; Deng, Chunhui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5357-db06804b0832db8a36cc8497f44b8118c949e88c6603e5fff3c522afbcbf36a83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Calibration</topic><topic>Catechin</topic><topic>Catechin - analysis</topic><topic>Catechins</topic><topic>Coffee, tea and other stimulative beverage industries</topic><topic>Electrophoresis, Capillary - instrumentation</topic><topic>Epicatechin</topic><topic>Extraction</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Green tea</topic><topic>Linearity</topic><topic>Medical sciences</topic><topic>Microwave-assisted extraction</topic><topic>Microwaves</topic><topic>Molecular Conformation</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Reproducibility</topic><topic>Reproducibility of Results</topic><topic>Separation</topic><topic>Stereoisomerism</topic><topic>Tea - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Zhongbo</creatorcontrib><creatorcontrib>Huang, Danni</creatorcontrib><creatorcontrib>Tang, Zhuxin</creatorcontrib><creatorcontrib>Deng, Chunhui</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Zhongbo</au><au>Huang, Danni</au><au>Tang, Zhuxin</au><au>Deng, Chunhui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Microwave-assisted extraction followed by CE for determination of catechin and epicatechin in green tea</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2010-04</date><risdate>2010</risdate><volume>33</volume><issue>8</issue><spage>1079</spage><epage>1084</epage><pages>1079-1084</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>In this work, for the first time, microwave-assisted extraction (MAE) followed by CE was developed for the fast analysis of catechin and epicatechin in green tea. In the proposed method, catechin and epicatechin in green tea samples were rapidly extracted by MAE technique, and then analyzed by CE. The MAE conditions and the method's validation were studied. It is found that the extraction time of 1 min with 400 W microwave irradiation is enough to completely extract catechin and epicatechin in green tea sample, whereas the conventional ultrasonic extraction (USE) technique needs long extraction time of 60 min. The method validations were also studied in this work. The calibration curve shows good linearity in 0.01-3 mg/mL for catechin (R²=0.993), and 0.005-3 mg/mL for epicatechin (R²=0.996), respectively. The RSD values for catechin and epicatechin are 0.65 and 2.58%, respectively. This shows that the proposed method has good reproducibility. The proposed method has good recoveries, which are 118% for catechin and 120% for epicatechin. The proposed method was successfully applied to determination of the catechin and epicatechin in different green tea samples. The experiment results have demonstrated that the MAE following CE is a simple, fast and reliable method for the determination of catechin and epicatechin in green tea.</abstract><cop>Weinheim</cop><pub>Wiley-VCH Verlag</pub><pmid>20175087</pmid><doi>10.1002/jssc.200900647</doi><tpages>6</tpages></addata></record> |
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| subjects | Biological and medical sciences Calibration Catechin Catechin - analysis Catechins Coffee, tea and other stimulative beverage industries Electrophoresis, Capillary - instrumentation Epicatechin Extraction Food industries Fundamental and applied biological sciences. Psychology General pharmacology Green tea Linearity Medical sciences Microwave-assisted extraction Microwaves Molecular Conformation Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Reproducibility Reproducibility of Results Separation Stereoisomerism Tea - chemistry |
| title | Microwave-assisted extraction followed by CE for determination of catechin and epicatechin in green tea |
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