Gold Nanoparticle-Fluorophore Complexes: Sensitive and Discerning "Noses" for Biosystems Sensing
Gold nanoparticles (NPs) efficiently quench adsorbed fluorophores. Upon disruption of such complexes by an analyte, fluorescence turn‐on is observed. By judicious choice of the functionalized NP and the fluorophore, these complexes display different responses to analytes, thus leading to versatile y...
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| Veröffentlicht in: | Angewandte Chemie International Edition 2010-04, Vol.49 (19), p.3268-3279 |
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| creator | Bunz, Uwe H. F. Rotello, Vincent M. |
| description | Gold nanoparticles (NPs) efficiently quench adsorbed fluorophores. Upon disruption of such complexes by an analyte, fluorescence turn‐on is observed. By judicious choice of the functionalized NP and the fluorophore, these complexes display different responses to analytes, thus leading to versatile yet simple array‐based sensor platforms. Using this strategy, we can identify proteins in buffer and serum, distinguish between both different species and different strains of bacteria, and differentiate between healthy, cancerous, and metastatic human and murine cells.
Disruption desired: Different monolayer‐protected nanoparticle–fluorophore constructs are used in indicator‐displacement assays to spy on proteins, bacteria, cells, and ions (see picture). The modus operandi involves disruption of the preformed quencher–fluorophore complexes, leading to partial and analyte‐dependent fluorescence turn‐on. Small libraries of nanoparticle–fluorophore complexes get the sensing job for different biological analytes done. |
| doi_str_mv | 10.1002/anie.200906928 |
| format | Article |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animals Bacteria - isolation & purification Biosensing Techniques biosensors Diphosphates - chemistry displacement assays Fluorescent Dyes - chemistry fluorescent probes Gold - chemistry Humans Metal Nanoparticles - chemistry Mice Microarray Analysis nanoparticles Polymers - chemistry Proteins - chemistry |
| title | Gold Nanoparticle-Fluorophore Complexes: Sensitive and Discerning "Noses" for Biosystems Sensing |
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