Effect of urea on protein separation by ion-exchange chromatography

Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each pro...

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Veröffentlicht in:	Journal of biochemistry (Tokyo) 2010-05, Vol.147 (5), p.735-741
Hauptverfasser:	Khademi, Fatemeh, Mostafaie, Ali
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Chickens chromatography Chromatography, Ion Exchange Cysteine Endopeptidases - chemistry Cysteine Endopeptidases - isolation & purification efficiency Egg White - chemistry Female Fruit - enzymology Humans Plant Extracts - chemistry Pregnancy Proteins - chemistry Proteins - isolation & purification resolution selectivity urea Urea - chemistry
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