Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing
Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1992-12, Vol.258 (5089), p.1775-1780 |
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| container_title | Science (American Association for the Advancement of Science) |
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| creator | Hannon, G.J. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) Maroney, P.A Yu, Y.T Hannon, G.E Nilsent, T.W |
| description | Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing |
| doi_str_mv | 10.1126/science.1465612 |
| format | Article |
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(Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) ; Maroney, P.A ; Yu, Y.T ; Hannon, G.E ; Nilsent, T.W</creator><creatorcontrib>Hannon, G.J. (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) ; Maroney, P.A ; Yu, Y.T ; Hannon, G.E ; Nilsent, T.W</creatorcontrib><description>Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.1465612</identifier><identifier>PMID: 1465612</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Society for the Advancement of Science</publisher><subject>Analysis ; Animals ; ARN ; ASCARIS ; Ascaris - genetics ; Base Sequence ; Binding Sites ; Biological and medical sciences ; CAENORHABDITIS ELEGANS ; Exons ; Fundamental and applied biological sciences. Psychology ; Gels ; Genetic engineering ; Models, Structural ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Molecules ; Nematodes ; Nucleic Acid Conformation ; NUCLEOTIDE ; Nucleotide sequence ; Nucleotides ; NUCLEOTIDOS ; Purines ; RNA ; RNA Splicing ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; RNA, Small Nuclear - chemistry ; RNA, Small Nuclear - genetics ; RNA, Small Nuclear - metabolism ; Small nuclear RNA ; Spliced leader RNA ; Trans splicing ; Transcription. Transcription factor. Splicing. 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(Cold Spring Harbor Laboratory, Cold Spring Harbor, NY)</creatorcontrib><creatorcontrib>Maroney, P.A</creatorcontrib><creatorcontrib>Yu, Y.T</creatorcontrib><creatorcontrib>Hannon, G.E</creatorcontrib><creatorcontrib>Nilsent, T.W</creatorcontrib><title>Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Nematode trans-spliced leader (SL) RNAs are composed of two domains, an exon [the 22-nucleotide spliced leader] and a small nuclear RNA (snRNA)-like sequence. Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing</description><subject>Analysis</subject><subject>Animals</subject><subject>ARN</subject><subject>ASCARIS</subject><subject>Ascaris - genetics</subject><subject>Base Sequence</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>CAENORHABDITIS ELEGANS</subject><subject>Exons</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genetic engineering</subject><subject>Models, Structural</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Molecules</subject><subject>Nematodes</subject><subject>Nucleic Acid Conformation</subject><subject>NUCLEOTIDE</subject><subject>Nucleotide sequence</subject><subject>Nucleotides</subject><subject>NUCLEOTIDOS</subject><subject>Purines</subject><subject>RNA</subject><subject>RNA Splicing</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Nuclear - chemistry</subject><subject>RNA, Small Nuclear - genetics</subject><subject>RNA, Small Nuclear - metabolism</subject><subject>Small nuclear RNA</subject><subject>Spliced leader RNA</subject><subject>Trans splicing</subject><subject>Transcription. Transcription factor. Splicing. 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(Cold Spring Harbor Laboratory, Cold Spring Harbor, NY) ; Maroney, P.A ; Yu, Y.T ; Hannon, G.E ; Nilsent, T.W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c582t-798ec4e3116e0a9e53dade270669cb3eaf799ed21ac2cf60dd0db682e077b6703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Analysis</topic><topic>Animals</topic><topic>ARN</topic><topic>ASCARIS</topic><topic>Ascaris - genetics</topic><topic>Base Sequence</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>CAENORHABDITIS ELEGANS</topic><topic>Exons</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>Genetic engineering</topic><topic>Models, Structural</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Molecules</topic><topic>Nematodes</topic><topic>Nucleic Acid Conformation</topic><topic>NUCLEOTIDE</topic><topic>Nucleotide sequence</topic><topic>Nucleotides</topic><topic>NUCLEOTIDOS</topic><topic>Purines</topic><topic>RNA</topic><topic>RNA Splicing</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Nuclear - chemistry</topic><topic>RNA, Small Nuclear - genetics</topic><topic>RNA, Small Nuclear - metabolism</topic><topic>Small nuclear RNA</topic><topic>Spliced leader RNA</topic><topic>Trans splicing</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hannon, G.J. 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Participation in vitro of the spliced leader RNA in trans-splicing reactions is independent of the exon sequence or size and instead depends on features contained in the snRNA-like domain of the molecule. Chemical modification interference analysis has revealed that two short sequence elements in the snRNA-like domain are necessary for SL RNA activity. These elements are sufficient for such activity because when added to a 72-nucleotide fragment of a nematode U1 snRNA, this hybrid RNA could participate in trans-splicing reactions in vitro. One of the critical sequence elements may function by base-pairing with U6 snRNA, an essential U snRNA for both cis- and trans-splicing</abstract><cop>Washington, DC</cop><pub>American Society for the Advancement of Science</pub><pmid>1465612</pmid><doi>10.1126/science.1465612</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1992-12, Vol.258 (5089), p.1775-1780 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73390072 |
| source | American Association for the Advancement of Science; Jstor Complete Legacy; MEDLINE |
| subjects | Analysis Animals ARN ASCARIS Ascaris - genetics Base Sequence Binding Sites Biological and medical sciences CAENORHABDITIS ELEGANS Exons Fundamental and applied biological sciences. Psychology Gels Genetic engineering Models, Structural Molecular and cellular biology Molecular genetics Molecular Sequence Data Molecules Nematodes Nucleic Acid Conformation NUCLEOTIDE Nucleotide sequence Nucleotides NUCLEOTIDOS Purines RNA RNA Splicing RNA, Messenger - genetics RNA, Messenger - metabolism RNA, Small Nuclear - chemistry RNA, Small Nuclear - genetics RNA, Small Nuclear - metabolism Small nuclear RNA Spliced leader RNA Trans splicing Transcription. Transcription factor. Splicing. Rna processing |
| title | Interaction of U6 snRNA with a sequence required for function of the nematode SL RNA in trans-splicing |
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