Differential gene expression of proinflammatory chemokines and cytokines in lungs of ascites-resistant and -susceptible broiler chickens following intravenous cellulose microparticle injection
Intravenous injection of microparticles (MPs) is a tool to reveal susceptibility to pulmonary hypertension (PH) syndrome (PHS, ascites) in broilers. After injection MPs get lodged in pulmonary arterioles and cause localized inflammation. To examine the expression of chemokines/cytokines during the M...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2010-02, Vol.133 (2), p.250-255 |
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| creator | Hamal, Krishna R. Wideman, Robert F. Anthony, Nicholas B. Erf, Gisela F. |
| description | Intravenous injection of microparticles (MPs) is a tool to reveal susceptibility to pulmonary hypertension (PH) syndrome (PHS, ascites) in broilers. After injection MPs get lodged in pulmonary arterioles and cause localized inflammation. To examine the expression of chemokines/cytokines during the MP-induced pulmonary inflammatory response, lungs were collected from 4-week-old broilers (6/line/time point) from the PHS-resistant (RES) and -susceptible (SUS) broilers before (0
h) and after (2, 6, 12, 24, and 48
h) MP injection and analyzed using quantitative RT-PCR. In both lines, expression of interleukin-1β (IL-1β), IL-6, IL-8, and K60 increased from 0 to 6
h, reached peak levels at 6 and 12
h, and decreased thereafter, whereas IL-4 and interferon gamma (IFN-γ) expression remained elevated past 12
h. Lungs from the RES line broilers had higher expression (
P
<
0.05) of IL-1β and IL-6 at 2, 6, and 12
h; higher IL-8 at 6 and 12
h; higher K60 at 6, 12, and 24
h; higher IL-4 at 12, 24, and 48
h and higher IFN-γ expression at 6 and 48
h post-MP injection than SUS line broilers. Higher expression of chemokines/cytokines in RES compared to SUS line lungs may explain the ability of RES line broilers to effectively counteract the MP-induced PH and resolve the vascular occlusion. |
| doi_str_mv | 10.1016/j.vetimm.2009.07.011 |
| format | Article |
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h) and after (2, 6, 12, 24, and 48
h) MP injection and analyzed using quantitative RT-PCR. In both lines, expression of interleukin-1β (IL-1β), IL-6, IL-8, and K60 increased from 0 to 6
h, reached peak levels at 6 and 12
h, and decreased thereafter, whereas IL-4 and interferon gamma (IFN-γ) expression remained elevated past 12
h. Lungs from the RES line broilers had higher expression (
P
<
0.05) of IL-1β and IL-6 at 2, 6, and 12
h; higher IL-8 at 6 and 12
h; higher K60 at 6, 12, and 24
h; higher IL-4 at 12, 24, and 48
h and higher IFN-γ expression at 6 and 48
h post-MP injection than SUS line broilers. Higher expression of chemokines/cytokines in RES compared to SUS line lungs may explain the ability of RES line broilers to effectively counteract the MP-induced PH and resolve the vascular occlusion.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2009.07.011</identifier><identifier>PMID: 19698998</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; ascites ; Ascites - genetics ; Ascites - immunology ; Ascites - veterinary ; Base Sequence ; broiler chickens ; Broilers ; cellulose ; Cellulose - administration & dosage ; cellulose microparticles ; Chemokine production ; chemokines ; Chemokines - genetics ; Chickens - genetics ; Chickens - immunology ; Cytokine production ; cytokines ; Cytokines - genetics ; disease resistance ; DNA Primers - genetics ; Gene Expression ; Hypertension, Pulmonary - etiology ; Hypertension, Pulmonary - genetics ; Hypertension, Pulmonary - immunology ; Hypertension, Pulmonary - veterinary ; Inflammation ; Inflammation Mediators - metabolism ; Injections, Intravenous ; intravenous injection ; Intravenous microparticle injection ; Lung - immunology ; lungs ; Male ; Particle Size ; Perivascular mononuclear cell aggregates ; Poultry Diseases - etiology ; Poultry Diseases - genetics ; Poultry Diseases - immunology ; proinflammatory cytokines ; Pulmonary hypertension syndrome ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Syndrome ; tissue analysis</subject><ispartof>Veterinary immunology and immunopathology, 2010-02, Vol.133 (2), p.250-255</ispartof><rights>2009 Elsevier B.V.</rights><rights>Copyright 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-133dcd232b9fc44edf0ebe93187d4ecd69174cb7f1b93a10aad838290ea31f713</citedby><cites>FETCH-LOGICAL-c451t-133dcd232b9fc44edf0ebe93187d4ecd69174cb7f1b93a10aad838290ea31f713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetimm.2009.07.011$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27926,27927,45997</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19698998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hamal, Krishna R.</creatorcontrib><creatorcontrib>Wideman, Robert F.</creatorcontrib><creatorcontrib>Anthony, Nicholas B.</creatorcontrib><creatorcontrib>Erf, Gisela F.</creatorcontrib><title>Differential gene expression of proinflammatory chemokines and cytokines in lungs of ascites-resistant and -susceptible broiler chickens following intravenous cellulose microparticle injection</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>Intravenous injection of microparticles (MPs) is a tool to reveal susceptibility to pulmonary hypertension (PH) syndrome (PHS, ascites) in broilers. After injection MPs get lodged in pulmonary arterioles and cause localized inflammation. To examine the expression of chemokines/cytokines during the MP-induced pulmonary inflammatory response, lungs were collected from 4-week-old broilers (6/line/time point) from the PHS-resistant (RES) and -susceptible (SUS) broilers before (0
h) and after (2, 6, 12, 24, and 48
h) MP injection and analyzed using quantitative RT-PCR. In both lines, expression of interleukin-1β (IL-1β), IL-6, IL-8, and K60 increased from 0 to 6
h, reached peak levels at 6 and 12
h, and decreased thereafter, whereas IL-4 and interferon gamma (IFN-γ) expression remained elevated past 12
h. Lungs from the RES line broilers had higher expression (
P
<
0.05) of IL-1β and IL-6 at 2, 6, and 12
h; higher IL-8 at 6 and 12
h; higher K60 at 6, 12, and 24
h; higher IL-4 at 12, 24, and 48
h and higher IFN-γ expression at 6 and 48
h post-MP injection than SUS line broilers. Higher expression of chemokines/cytokines in RES compared to SUS line lungs may explain the ability of RES line broilers to effectively counteract the MP-induced PH and resolve the vascular occlusion.</description><subject>Animals</subject><subject>ascites</subject><subject>Ascites - genetics</subject><subject>Ascites - immunology</subject><subject>Ascites - veterinary</subject><subject>Base Sequence</subject><subject>broiler chickens</subject><subject>Broilers</subject><subject>cellulose</subject><subject>Cellulose - administration & dosage</subject><subject>cellulose microparticles</subject><subject>Chemokine production</subject><subject>chemokines</subject><subject>Chemokines - genetics</subject><subject>Chickens - genetics</subject><subject>Chickens - immunology</subject><subject>Cytokine production</subject><subject>cytokines</subject><subject>Cytokines - genetics</subject><subject>disease resistance</subject><subject>DNA Primers - genetics</subject><subject>Gene Expression</subject><subject>Hypertension, Pulmonary - etiology</subject><subject>Hypertension, Pulmonary - genetics</subject><subject>Hypertension, Pulmonary - immunology</subject><subject>Hypertension, Pulmonary - veterinary</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Injections, Intravenous</subject><subject>intravenous injection</subject><subject>Intravenous microparticle injection</subject><subject>Lung - immunology</subject><subject>lungs</subject><subject>Male</subject><subject>Particle Size</subject><subject>Perivascular mononuclear cell aggregates</subject><subject>Poultry Diseases - etiology</subject><subject>Poultry Diseases - genetics</subject><subject>Poultry Diseases - immunology</subject><subject>proinflammatory cytokines</subject><subject>Pulmonary hypertension syndrome</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Syndrome</subject><subject>tissue analysis</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUGP0zAUhC0EYruFf4DAN04Jdpw28QUJLSwgrcQB9mw5znN5XccOtlPov-On4dJK3Dg9WZoZj-Yj5AVnNWd8-2ZfHyDjNNUNY7JmXc04f0RWvO9E1WxE-5isimxTNW3TXZHrlPaMsY3s-6fkisut7KXsV-T3e7QWIviM2tEdeKDwa46QEgZPg6VzDOit09Okc4hHar7DFB7QQ6Laj9Qc8-WFnrrF79LJpJPBDKkqOZiy9vmvtkpLMjBnHBzQoeQ6iCUPzQP4RG1wLvxEvytJOeoD-LAkasC5xYUEdEITw6xjRlPs6Pdgcun4jDyx2iV4frlrcn_74dvNp-ruy8fPN-_uKtNueK64EKMZG9EM0pq2hdEyGECKMtfYghm3knetGTrLByk0Z1qPvegbyUALbjsu1uT1ObcM8mOBlNWE6dROeyhFVSdE30smt0XZnpWlb0oRrJojTjoeFWfqhE7t1RmdOqFTrFMFXbG9vHywDBOM_0wXVkXw6iywOii9i5jU_deGccF4V05hviZvzwooQxwQoiocwBsYMZa11Bjw_x3-ADvbvaw</recordid><startdate>20100215</startdate><enddate>20100215</enddate><creator>Hamal, Krishna R.</creator><creator>Wideman, Robert F.</creator><creator>Anthony, Nicholas B.</creator><creator>Erf, Gisela F.</creator><general>Elsevier B.V</general><general>Amsterdam: Elsevier</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100215</creationdate><title>Differential gene expression of proinflammatory chemokines and cytokines in lungs of ascites-resistant and -susceptible broiler chickens following intravenous cellulose microparticle injection</title><author>Hamal, Krishna R. ; Wideman, Robert F. ; Anthony, Nicholas B. ; Erf, Gisela F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-133dcd232b9fc44edf0ebe93187d4ecd69174cb7f1b93a10aad838290ea31f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>ascites</topic><topic>Ascites - genetics</topic><topic>Ascites - immunology</topic><topic>Ascites - veterinary</topic><topic>Base Sequence</topic><topic>broiler chickens</topic><topic>Broilers</topic><topic>cellulose</topic><topic>Cellulose - administration & dosage</topic><topic>cellulose microparticles</topic><topic>Chemokine production</topic><topic>chemokines</topic><topic>Chemokines - genetics</topic><topic>Chickens - genetics</topic><topic>Chickens - immunology</topic><topic>Cytokine production</topic><topic>cytokines</topic><topic>Cytokines - genetics</topic><topic>disease resistance</topic><topic>DNA Primers - genetics</topic><topic>Gene Expression</topic><topic>Hypertension, Pulmonary - etiology</topic><topic>Hypertension, Pulmonary - genetics</topic><topic>Hypertension, Pulmonary - immunology</topic><topic>Hypertension, Pulmonary - veterinary</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Injections, Intravenous</topic><topic>intravenous injection</topic><topic>Intravenous microparticle injection</topic><topic>Lung - immunology</topic><topic>lungs</topic><topic>Male</topic><topic>Particle Size</topic><topic>Perivascular mononuclear cell aggregates</topic><topic>Poultry Diseases - etiology</topic><topic>Poultry Diseases - genetics</topic><topic>Poultry Diseases - immunology</topic><topic>proinflammatory cytokines</topic><topic>Pulmonary hypertension syndrome</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Syndrome</topic><topic>tissue analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hamal, Krishna R.</creatorcontrib><creatorcontrib>Wideman, Robert F.</creatorcontrib><creatorcontrib>Anthony, Nicholas B.</creatorcontrib><creatorcontrib>Erf, Gisela F.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hamal, Krishna R.</au><au>Wideman, Robert F.</au><au>Anthony, Nicholas B.</au><au>Erf, Gisela F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential gene expression of proinflammatory chemokines and cytokines in lungs of ascites-resistant and -susceptible broiler chickens following intravenous cellulose microparticle injection</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2010-02-15</date><risdate>2010</risdate><volume>133</volume><issue>2</issue><spage>250</spage><epage>255</epage><pages>250-255</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>Intravenous injection of microparticles (MPs) is a tool to reveal susceptibility to pulmonary hypertension (PH) syndrome (PHS, ascites) in broilers. After injection MPs get lodged in pulmonary arterioles and cause localized inflammation. To examine the expression of chemokines/cytokines during the MP-induced pulmonary inflammatory response, lungs were collected from 4-week-old broilers (6/line/time point) from the PHS-resistant (RES) and -susceptible (SUS) broilers before (0
h) and after (2, 6, 12, 24, and 48
h) MP injection and analyzed using quantitative RT-PCR. In both lines, expression of interleukin-1β (IL-1β), IL-6, IL-8, and K60 increased from 0 to 6
h, reached peak levels at 6 and 12
h, and decreased thereafter, whereas IL-4 and interferon gamma (IFN-γ) expression remained elevated past 12
h. Lungs from the RES line broilers had higher expression (
P
<
0.05) of IL-1β and IL-6 at 2, 6, and 12
h; higher IL-8 at 6 and 12
h; higher K60 at 6, 12, and 24
h; higher IL-4 at 12, 24, and 48
h and higher IFN-γ expression at 6 and 48
h post-MP injection than SUS line broilers. Higher expression of chemokines/cytokines in RES compared to SUS line lungs may explain the ability of RES line broilers to effectively counteract the MP-induced PH and resolve the vascular occlusion.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>19698998</pmid><doi>10.1016/j.vetimm.2009.07.011</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Animals ascites Ascites - genetics Ascites - immunology Ascites - veterinary Base Sequence broiler chickens Broilers cellulose Cellulose - administration & dosage cellulose microparticles Chemokine production chemokines Chemokines - genetics Chickens - genetics Chickens - immunology Cytokine production cytokines Cytokines - genetics disease resistance DNA Primers - genetics Gene Expression Hypertension, Pulmonary - etiology Hypertension, Pulmonary - genetics Hypertension, Pulmonary - immunology Hypertension, Pulmonary - veterinary Inflammation Inflammation Mediators - metabolism Injections, Intravenous intravenous injection Intravenous microparticle injection Lung - immunology lungs Male Particle Size Perivascular mononuclear cell aggregates Poultry Diseases - etiology Poultry Diseases - genetics Poultry Diseases - immunology proinflammatory cytokines Pulmonary hypertension syndrome RNA, Messenger - genetics RNA, Messenger - metabolism Syndrome tissue analysis |
| title | Differential gene expression of proinflammatory chemokines and cytokines in lungs of ascites-resistant and -susceptible broiler chickens following intravenous cellulose microparticle injection |
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