C-terminal sequencing of peptides using electrospray ionization mass spectrometry
A low‐flow reactor is described for the on‐line monitoring of peptides digested with carboxypeptidase P by electrospray ionization. Two peptides were analyzed using this technique: glucagon (average MW 3482.8 Da), and apomyoglobin (average MW 16 951.5). Both peptides gave interpretable results. The...
Gespeichert in:
| Veröffentlicht in: | Rapid communications in mass spectrometry 1992-11, Vol.6 (11), p.637-640 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 640 |
|---|---|
| container_issue | 11 |
| container_start_page | 637 |
| container_title | Rapid communications in mass spectrometry |
| container_volume | 6 |
| creator | Rosnack, Kenneth J. Stroh, Justin G. |
| description | A low‐flow reactor is described for the on‐line monitoring of peptides digested with carboxypeptidase P by electrospray ionization. Two peptides were analyzed using this technique: glucagon (average MW 3482.8 Da), and apomyoglobin (average MW 16 951.5). Both peptides gave interpretable results. The first 19 amino acids of glucagon were successfully sequenced. Apomyoglobin yielded sequence information to the 30th amino acid with some gaps. At 300 nL/min, 50% of the first 30 amino acids were sequenced and at 1μL/min, 67% of the first 30 amino acids were observed. |
| doi_str_mv | 10.1002/rcm.1290061102 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73388487</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73388487</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4072-7d38b045cca19d878dbeffe0e039206278bdbbb916ae972b1324446fdcb02c6f3</originalsourceid><addsrcrecordid>eNqFkEtv1DAUhS0EaofCll2lLFB3mV4_EttLNOoD1NJCgUpsLNu5QYa8amfUDr-eDBm1YsXqSj7fOT46hLyhsKQA7Dj6dkmZBigpBfaMLChomQPj9DlZgC5oLqhW--RlSj8BKC0Y7JE9KkpZCLUgn1b5iLENnW2yhHdr7HzofmR9nQ04jKHClK3T9gUb9GPs0xDtJgt9F37bcTpZa1PK0vBXbHGMm1fkRW2bhK9394B8PT35sjrPL67O3q_eXeRegGS5rLhyIArvLdWVkqpyWNcICFwzKJlUrnLOaVpa1JI5ypkQoqwr74D5suYH5GjOHWI_9U6jaUPy2DS2w36djORcKaHkBC5n0E_1U8TaDDG0Nm4MBbPd0EwbmqcNJ8PhLnntWqye8Hm0SX-7023ytqmjnUZLj5gQuhB0G6Nn7D40uPnPp-bz6vKfCvnsDWnEh0evjb9MKbkszO3HM3Orvt-UN9-uzQf-B1dOmwU</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73388487</pqid></control><display><type>article</type><title>C-terminal sequencing of peptides using electrospray ionization mass spectrometry</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Rosnack, Kenneth J. ; Stroh, Justin G.</creator><creatorcontrib>Rosnack, Kenneth J. ; Stroh, Justin G.</creatorcontrib><description>A low‐flow reactor is described for the on‐line monitoring of peptides digested with carboxypeptidase P by electrospray ionization. Two peptides were analyzed using this technique: glucagon (average MW 3482.8 Da), and apomyoglobin (average MW 16 951.5). Both peptides gave interpretable results. The first 19 amino acids of glucagon were successfully sequenced. Apomyoglobin yielded sequence information to the 30th amino acid with some gaps. At 300 nL/min, 50% of the first 30 amino acids were sequenced and at 1μL/min, 67% of the first 30 amino acids were observed.</description><identifier>ISSN: 0951-4198</identifier><identifier>EISSN: 1097-0231</identifier><identifier>DOI: 10.1002/rcm.1290061102</identifier><identifier>PMID: 1467548</identifier><language>eng</language><publisher>London: Heyden & Son Limited</publisher><subject>Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Apoproteins - genetics ; Biological and medical sciences ; Carboxypeptidases ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Glucagon - genetics ; Mass Spectrometry - methods ; Molecular Sequence Data ; Myoglobin - genetics ; Proteins</subject><ispartof>Rapid communications in mass spectrometry, 1992-11, Vol.6 (11), p.637-640</ispartof><rights>Copyright © 1992 John Wiley & Sons, Ltd.</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4072-7d38b045cca19d878dbeffe0e039206278bdbbb916ae972b1324446fdcb02c6f3</citedby><cites>FETCH-LOGICAL-c4072-7d38b045cca19d878dbeffe0e039206278bdbbb916ae972b1324446fdcb02c6f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Frcm.1290061102$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Frcm.1290061102$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4495412$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1467548$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rosnack, Kenneth J.</creatorcontrib><creatorcontrib>Stroh, Justin G.</creatorcontrib><title>C-terminal sequencing of peptides using electrospray ionization mass spectrometry</title><title>Rapid communications in mass spectrometry</title><addtitle>Rapid Commun. Mass Spectrom</addtitle><description>A low‐flow reactor is described for the on‐line monitoring of peptides digested with carboxypeptidase P by electrospray ionization. Two peptides were analyzed using this technique: glucagon (average MW 3482.8 Da), and apomyoglobin (average MW 16 951.5). Both peptides gave interpretable results. The first 19 amino acids of glucagon were successfully sequenced. Apomyoglobin yielded sequence information to the 30th amino acid with some gaps. At 300 nL/min, 50% of the first 30 amino acids were sequenced and at 1μL/min, 67% of the first 30 amino acids were observed.</description><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Apoproteins - genetics</subject><subject>Biological and medical sciences</subject><subject>Carboxypeptidases</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Glucagon - genetics</subject><subject>Mass Spectrometry - methods</subject><subject>Molecular Sequence Data</subject><subject>Myoglobin - genetics</subject><subject>Proteins</subject><issn>0951-4198</issn><issn>1097-0231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAUhS0EaofCll2lLFB3mV4_EttLNOoD1NJCgUpsLNu5QYa8amfUDr-eDBm1YsXqSj7fOT46hLyhsKQA7Dj6dkmZBigpBfaMLChomQPj9DlZgC5oLqhW--RlSj8BKC0Y7JE9KkpZCLUgn1b5iLENnW2yhHdr7HzofmR9nQ04jKHClK3T9gUb9GPs0xDtJgt9F37bcTpZa1PK0vBXbHGMm1fkRW2bhK9394B8PT35sjrPL67O3q_eXeRegGS5rLhyIArvLdWVkqpyWNcICFwzKJlUrnLOaVpa1JI5ypkQoqwr74D5suYH5GjOHWI_9U6jaUPy2DS2w36djORcKaHkBC5n0E_1U8TaDDG0Nm4MBbPd0EwbmqcNJ8PhLnntWqye8Hm0SX-7023ytqmjnUZLj5gQuhB0G6Nn7D40uPnPp-bz6vKfCvnsDWnEh0evjb9MKbkszO3HM3Orvt-UN9-uzQf-B1dOmwU</recordid><startdate>199211</startdate><enddate>199211</enddate><creator>Rosnack, Kenneth J.</creator><creator>Stroh, Justin G.</creator><general>Heyden & Son Limited</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199211</creationdate><title>C-terminal sequencing of peptides using electrospray ionization mass spectrometry</title><author>Rosnack, Kenneth J. ; Stroh, Justin G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4072-7d38b045cca19d878dbeffe0e039206278bdbbb916ae972b1324446fdcb02c6f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amino Acid Sequence</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Apoproteins - genetics</topic><topic>Biological and medical sciences</topic><topic>Carboxypeptidases</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Glucagon - genetics</topic><topic>Mass Spectrometry - methods</topic><topic>Molecular Sequence Data</topic><topic>Myoglobin - genetics</topic><topic>Proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rosnack, Kenneth J.</creatorcontrib><creatorcontrib>Stroh, Justin G.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Rapid communications in mass spectrometry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosnack, Kenneth J.</au><au>Stroh, Justin G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>C-terminal sequencing of peptides using electrospray ionization mass spectrometry</atitle><jtitle>Rapid communications in mass spectrometry</jtitle><addtitle>Rapid Commun. Mass Spectrom</addtitle><date>1992-11</date><risdate>1992</risdate><volume>6</volume><issue>11</issue><spage>637</spage><epage>640</epage><pages>637-640</pages><issn>0951-4198</issn><eissn>1097-0231</eissn><abstract>A low‐flow reactor is described for the on‐line monitoring of peptides digested with carboxypeptidase P by electrospray ionization. Two peptides were analyzed using this technique: glucagon (average MW 3482.8 Da), and apomyoglobin (average MW 16 951.5). Both peptides gave interpretable results. The first 19 amino acids of glucagon were successfully sequenced. Apomyoglobin yielded sequence information to the 30th amino acid with some gaps. At 300 nL/min, 50% of the first 30 amino acids were sequenced and at 1μL/min, 67% of the first 30 amino acids were observed.</abstract><cop>London</cop><pub>Heyden & Son Limited</pub><pmid>1467548</pmid><doi>10.1002/rcm.1290061102</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0951-4198 |
| ispartof | Rapid communications in mass spectrometry, 1992-11, Vol.6 (11), p.637-640 |
| issn | 0951-4198 1097-0231 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73388487 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acid Sequence Analytical, structural and metabolic biochemistry Apoproteins - genetics Biological and medical sciences Carboxypeptidases Fundamental and applied biological sciences. Psychology General aspects, investigation methods Glucagon - genetics Mass Spectrometry - methods Molecular Sequence Data Myoglobin - genetics Proteins |
| title | C-terminal sequencing of peptides using electrospray ionization mass spectrometry |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T09%3A49%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=C-terminal%20sequencing%20of%20peptides%20using%20electrospray%20ionization%20mass%20spectrometry&rft.jtitle=Rapid%20communications%20in%20mass%20spectrometry&rft.au=Rosnack,%20Kenneth%20J.&rft.date=1992-11&rft.volume=6&rft.issue=11&rft.spage=637&rft.epage=640&rft.pages=637-640&rft.issn=0951-4198&rft.eissn=1097-0231&rft_id=info:doi/10.1002/rcm.1290061102&rft_dat=%3Cproquest_cross%3E73388487%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73388487&rft_id=info:pmid/1467548&rfr_iscdi=true |