Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes
Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) product...
Gespeichert in:
| Veröffentlicht in: | Journal of Veterinary Medical Science 2010, Vol.72(1), pp.55-60 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 60 |
|---|---|
| container_issue | 1 |
| container_start_page | 55 |
| container_title | Journal of Veterinary Medical Science |
| container_volume | 72 |
| creator | Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine Sakonju, I Kanda, A Suzuki, T Kakuta, T Shimamura, S Okano, S Takase, K |
| description | Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) production, effectively eliminating invading agents. Reactivity against priming agents is known to vary with species; however, there have been few reports on the effects of priming agents on canine granulocytes. In the present study, we assayed the priming effects of lipopolysaccharide (LPS), recombinant canine tumor necrosis factor-alpha (rcTNF-alpha) and recombinant canine granulocyte macrophage colony-stimulating factor (rcGM-CSF) on canine granulocyte function in vitro. Isolated recombinant canine were primed with various concentrations of LPS, rcTNF-alpha and rcGM-CSF, and CD11b expression was assayed. Furthermore, actin polymerization, phagocytosis and ROS production were then assayed at primer concentrations where enhancement of CD11b expression was observed. LPS did not enhance canine granulocyte function. Phagocytosis and actin polymerization were not enhanced by priming agents; however, rcTNF-alpha and rcGM-CSF enhanced CD11b expression and ROS production in canine granulocytes. These results suggest that priming effects are mainly reflected in CD11b expression and ROS production, with rcGM-CSF and rcTNF-alpha having a priming effect similar to that observed in humans. |
| doi_str_mv | 10.1292/jvms.08-0494 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733881822</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3158676781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c562t-c73020c412a528634a605a2e5a2e740209a8e2627fd7172920c706edfb89ca113</originalsourceid><addsrcrecordid>eNpdkE1v1DAURS1ERYeBHVtQJBZsSHm2k9heQgW0qBJdgFhar449eEjsqZ0gzb_HUUZF6sIf0j2-ej6EvKJwQZliH_Z_x3wBsoZGNU_IhvJG1KLh6inZgKJdLVgL5-R5znsARptOPSPnVCnaciY35Ndt8qMPu8o6Z82Uq-iqwR_iIQ7HjMb8xuR7W2HoKx_cgOOIU0zHyhyn-McHWx6EymAo12qXMMxDLJHNL8iZwyHbl6dzS35--fzj8qq--f71-vLjTW3ajk21ERwYmIYybJnseIMdtMjsskRTIoXSso4J1wsqynfBCOhs7-6kMkgp35J3a-8hxfvZ5kmPPhs7DBhsnLMWnEtJJWOFfPuI3Mc5hTKcLlakYECLti15v1ImxZyTdfpQBGE6agp68a0X3xqkXnwX_M2pdL4bbf8fPgkuwKcV2OcJd_YBwDR5M9i1TTBNl-3U-hAu9rUNpeT1WuIwatwln_W32zIvAFAlGf8Hk3WcMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1468720174</pqid></control><display><type>article</type><title>Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine ; Sakonju, I ; Kanda, A ; Suzuki, T ; Kakuta, T ; Shimamura, S ; Okano, S ; Takase, K</creator><creatorcontrib>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine ; Sakonju, I ; Kanda, A ; Suzuki, T ; Kakuta, T ; Shimamura, S ; Okano, S ; Takase, K</creatorcontrib><description>Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) production, effectively eliminating invading agents. Reactivity against priming agents is known to vary with species; however, there have been few reports on the effects of priming agents on canine granulocytes. In the present study, we assayed the priming effects of lipopolysaccharide (LPS), recombinant canine tumor necrosis factor-alpha (rcTNF-alpha) and recombinant canine granulocyte macrophage colony-stimulating factor (rcGM-CSF) on canine granulocyte function in vitro. Isolated recombinant canine were primed with various concentrations of LPS, rcTNF-alpha and rcGM-CSF, and CD11b expression was assayed. Furthermore, actin polymerization, phagocytosis and ROS production were then assayed at primer concentrations where enhancement of CD11b expression was observed. LPS did not enhance canine granulocyte function. Phagocytosis and actin polymerization were not enhanced by priming agents; however, rcTNF-alpha and rcGM-CSF enhanced CD11b expression and ROS production in canine granulocytes. These results suggest that priming effects are mainly reflected in CD11b expression and ROS production, with rcGM-CSF and rcTNF-alpha having a priming effect similar to that observed in humans.</description><identifier>ISSN: 0916-7250</identifier><identifier>EISSN: 1347-7439</identifier><identifier>DOI: 10.1292/jvms.08-0494</identifier><identifier>PMID: 19915328</identifier><language>eng</language><publisher>Japan: JAPANESE SOCIETY OF VETERINARY SCIENCE</publisher><subject>Actins - metabolism ; Animals ; canine ; CD11b Antigen - genetics ; CD11b Antigen - metabolism ; Cells, Cultured ; CHIEN ; CITOQUINAS ; CYTOKINE ; CYTOKINES ; Cytokines - toxicity ; DOGS ; Gene Expression Regulation ; GRANULOCITOS ; GRANULOCYTE ; Granulocyte-Macrophage Colony-Stimulating Factor - toxicity ; GRANULOCYTES ; Granulocytes - drug effects ; http://www.fao.org/aos/agrovoc#c_11258 ; http://www.fao.org/aos/agrovoc#c_2352 ; http://www.fao.org/aos/agrovoc#c_24045 ; http://www.fao.org/aos/agrovoc#c_35078 ; http://www.fao.org/aos/agrovoc#c_3802 ; http://www.fao.org/aos/agrovoc#c_3856 ; IMMUNITE ; IMMUNITY ; INFLAMACION ; INFLAMMATION ; INMUNIDAD ; LIPOPOLISACARIDOS ; LIPOPOLYSACCHARIDE ; LIPOPOLYSACCHARIDES ; Lipopolysaccharides - toxicity ; LPS ; nflammatory cytokine ; PERRO ; Phagocytosis - drug effects ; priming ; Respiratory Burst ; Tumor Necrosis Factor-alpha - toxicity</subject><ispartof>Journal of Veterinary Medical Science, 2010, Vol.72(1), pp.55-60</ispartof><rights>2010 by the Japanese Society of Veterinary Science</rights><rights>Copyright Japan Science and Technology Agency 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c562t-c73020c412a528634a605a2e5a2e740209a8e2627fd7172920c706edfb89ca113</citedby><cites>FETCH-LOGICAL-c562t-c73020c412a528634a605a2e5a2e740209a8e2627fd7172920c706edfb89ca113</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,1883,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19915328$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine</creatorcontrib><creatorcontrib>Sakonju, I</creatorcontrib><creatorcontrib>Kanda, A</creatorcontrib><creatorcontrib>Suzuki, T</creatorcontrib><creatorcontrib>Kakuta, T</creatorcontrib><creatorcontrib>Shimamura, S</creatorcontrib><creatorcontrib>Okano, S</creatorcontrib><creatorcontrib>Takase, K</creatorcontrib><title>Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes</title><title>Journal of Veterinary Medical Science</title><addtitle>J. Vet. Med. Sci.</addtitle><description>Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) production, effectively eliminating invading agents. Reactivity against priming agents is known to vary with species; however, there have been few reports on the effects of priming agents on canine granulocytes. In the present study, we assayed the priming effects of lipopolysaccharide (LPS), recombinant canine tumor necrosis factor-alpha (rcTNF-alpha) and recombinant canine granulocyte macrophage colony-stimulating factor (rcGM-CSF) on canine granulocyte function in vitro. Isolated recombinant canine were primed with various concentrations of LPS, rcTNF-alpha and rcGM-CSF, and CD11b expression was assayed. Furthermore, actin polymerization, phagocytosis and ROS production were then assayed at primer concentrations where enhancement of CD11b expression was observed. LPS did not enhance canine granulocyte function. Phagocytosis and actin polymerization were not enhanced by priming agents; however, rcTNF-alpha and rcGM-CSF enhanced CD11b expression and ROS production in canine granulocytes. These results suggest that priming effects are mainly reflected in CD11b expression and ROS production, with rcGM-CSF and rcTNF-alpha having a priming effect similar to that observed in humans.</description><subject>Actins - metabolism</subject><subject>Animals</subject><subject>canine</subject><subject>CD11b Antigen - genetics</subject><subject>CD11b Antigen - metabolism</subject><subject>Cells, Cultured</subject><subject>CHIEN</subject><subject>CITOQUINAS</subject><subject>CYTOKINE</subject><subject>CYTOKINES</subject><subject>Cytokines - toxicity</subject><subject>DOGS</subject><subject>Gene Expression Regulation</subject><subject>GRANULOCITOS</subject><subject>GRANULOCYTE</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - toxicity</subject><subject>GRANULOCYTES</subject><subject>Granulocytes - drug effects</subject><subject>http://www.fao.org/aos/agrovoc#c_11258</subject><subject>http://www.fao.org/aos/agrovoc#c_2352</subject><subject>http://www.fao.org/aos/agrovoc#c_24045</subject><subject>http://www.fao.org/aos/agrovoc#c_35078</subject><subject>http://www.fao.org/aos/agrovoc#c_3802</subject><subject>http://www.fao.org/aos/agrovoc#c_3856</subject><subject>IMMUNITE</subject><subject>IMMUNITY</subject><subject>INFLAMACION</subject><subject>INFLAMMATION</subject><subject>INMUNIDAD</subject><subject>LIPOPOLISACARIDOS</subject><subject>LIPOPOLYSACCHARIDE</subject><subject>LIPOPOLYSACCHARIDES</subject><subject>Lipopolysaccharides - toxicity</subject><subject>LPS</subject><subject>nflammatory cytokine</subject><subject>PERRO</subject><subject>Phagocytosis - drug effects</subject><subject>priming</subject><subject>Respiratory Burst</subject><subject>Tumor Necrosis Factor-alpha - toxicity</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkE1v1DAURS1ERYeBHVtQJBZsSHm2k9heQgW0qBJdgFhar449eEjsqZ0gzb_HUUZF6sIf0j2-ej6EvKJwQZliH_Z_x3wBsoZGNU_IhvJG1KLh6inZgKJdLVgL5-R5znsARptOPSPnVCnaciY35Ndt8qMPu8o6Z82Uq-iqwR_iIQ7HjMb8xuR7W2HoKx_cgOOIU0zHyhyn-McHWx6EymAo12qXMMxDLJHNL8iZwyHbl6dzS35--fzj8qq--f71-vLjTW3ajk21ERwYmIYybJnseIMdtMjsskRTIoXSso4J1wsqynfBCOhs7-6kMkgp35J3a-8hxfvZ5kmPPhs7DBhsnLMWnEtJJWOFfPuI3Mc5hTKcLlakYECLti15v1ImxZyTdfpQBGE6agp68a0X3xqkXnwX_M2pdL4bbf8fPgkuwKcV2OcJd_YBwDR5M9i1TTBNl-3U-hAu9rUNpeT1WuIwatwln_W32zIvAFAlGf8Hk3WcMg</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine</creator><creator>Sakonju, I</creator><creator>Kanda, A</creator><creator>Suzuki, T</creator><creator>Kakuta, T</creator><creator>Shimamura, S</creator><creator>Okano, S</creator><creator>Takase, K</creator><general>JAPANESE SOCIETY OF VETERINARY SCIENCE</general><general>Japan Science and Technology Agency</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QR</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes</title><author>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine ; Sakonju, I ; Kanda, A ; Suzuki, T ; Kakuta, T ; Shimamura, S ; Okano, S ; Takase, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c562t-c73020c412a528634a605a2e5a2e740209a8e2627fd7172920c706edfb89ca113</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Actins - metabolism</topic><topic>Animals</topic><topic>canine</topic><topic>CD11b Antigen - genetics</topic><topic>CD11b Antigen - metabolism</topic><topic>Cells, Cultured</topic><topic>CHIEN</topic><topic>CITOQUINAS</topic><topic>CYTOKINE</topic><topic>CYTOKINES</topic><topic>Cytokines - toxicity</topic><topic>DOGS</topic><topic>Gene Expression Regulation</topic><topic>GRANULOCITOS</topic><topic>GRANULOCYTE</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - toxicity</topic><topic>GRANULOCYTES</topic><topic>Granulocytes - drug effects</topic><topic>http://www.fao.org/aos/agrovoc#c_11258</topic><topic>http://www.fao.org/aos/agrovoc#c_2352</topic><topic>http://www.fao.org/aos/agrovoc#c_24045</topic><topic>http://www.fao.org/aos/agrovoc#c_35078</topic><topic>http://www.fao.org/aos/agrovoc#c_3802</topic><topic>http://www.fao.org/aos/agrovoc#c_3856</topic><topic>IMMUNITE</topic><topic>IMMUNITY</topic><topic>INFLAMACION</topic><topic>INFLAMMATION</topic><topic>INMUNIDAD</topic><topic>LIPOPOLISACARIDOS</topic><topic>LIPOPOLYSACCHARIDE</topic><topic>LIPOPOLYSACCHARIDES</topic><topic>Lipopolysaccharides - toxicity</topic><topic>LPS</topic><topic>nflammatory cytokine</topic><topic>PERRO</topic><topic>Phagocytosis - drug effects</topic><topic>priming</topic><topic>Respiratory Burst</topic><topic>Tumor Necrosis Factor-alpha - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine</creatorcontrib><creatorcontrib>Sakonju, I</creatorcontrib><creatorcontrib>Kanda, A</creatorcontrib><creatorcontrib>Suzuki, T</creatorcontrib><creatorcontrib>Kakuta, T</creatorcontrib><creatorcontrib>Shimamura, S</creatorcontrib><creatorcontrib>Okano, S</creatorcontrib><creatorcontrib>Takase, K</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Chemoreception Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Veterinary Medical Science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maeda, K., Kitasato Univ., Towada, Aomori (Japan). School of Veterinary Medicine</au><au>Sakonju, I</au><au>Kanda, A</au><au>Suzuki, T</au><au>Kakuta, T</au><au>Shimamura, S</au><au>Okano, S</au><au>Takase, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes</atitle><jtitle>Journal of Veterinary Medical Science</jtitle><addtitle>J. Vet. Med. Sci.</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>72</volume><issue>1</issue><spage>55</spage><epage>60</epage><pages>55-60</pages><issn>0916-7250</issn><eissn>1347-7439</eissn><abstract>Granulocytes play a pivotal role in natural immunity. Under inflammatory conditions, granulocytes are universally primed by several agents, such as endotoxins and inflammatory cytokines. Primed granulocytes exert potent adhesiveness, chemotaxis, phagocytosis and reactive oxygen species (ROS) production, effectively eliminating invading agents. Reactivity against priming agents is known to vary with species; however, there have been few reports on the effects of priming agents on canine granulocytes. In the present study, we assayed the priming effects of lipopolysaccharide (LPS), recombinant canine tumor necrosis factor-alpha (rcTNF-alpha) and recombinant canine granulocyte macrophage colony-stimulating factor (rcGM-CSF) on canine granulocyte function in vitro. Isolated recombinant canine were primed with various concentrations of LPS, rcTNF-alpha and rcGM-CSF, and CD11b expression was assayed. Furthermore, actin polymerization, phagocytosis and ROS production were then assayed at primer concentrations where enhancement of CD11b expression was observed. LPS did not enhance canine granulocyte function. Phagocytosis and actin polymerization were not enhanced by priming agents; however, rcTNF-alpha and rcGM-CSF enhanced CD11b expression and ROS production in canine granulocytes. These results suggest that priming effects are mainly reflected in CD11b expression and ROS production, with rcGM-CSF and rcTNF-alpha having a priming effect similar to that observed in humans.</abstract><cop>Japan</cop><pub>JAPANESE SOCIETY OF VETERINARY SCIENCE</pub><pmid>19915328</pmid><doi>10.1292/jvms.08-0494</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0916-7250 |
| ispartof | Journal of Veterinary Medical Science, 2010, Vol.72(1), pp.55-60 |
| issn | 0916-7250 1347-7439 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733881822 |
| source | J-STAGE Free; MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Actins - metabolism Animals canine CD11b Antigen - genetics CD11b Antigen - metabolism Cells, Cultured CHIEN CITOQUINAS CYTOKINE CYTOKINES Cytokines - toxicity DOGS Gene Expression Regulation GRANULOCITOS GRANULOCYTE Granulocyte-Macrophage Colony-Stimulating Factor - toxicity GRANULOCYTES Granulocytes - drug effects http://www.fao.org/aos/agrovoc#c_11258 http://www.fao.org/aos/agrovoc#c_2352 http://www.fao.org/aos/agrovoc#c_24045 http://www.fao.org/aos/agrovoc#c_35078 http://www.fao.org/aos/agrovoc#c_3802 http://www.fao.org/aos/agrovoc#c_3856 IMMUNITE IMMUNITY INFLAMACION INFLAMMATION INMUNIDAD LIPOPOLISACARIDOS LIPOPOLYSACCHARIDE LIPOPOLYSACCHARIDES Lipopolysaccharides - toxicity LPS nflammatory cytokine PERRO Phagocytosis - drug effects priming Respiratory Burst Tumor Necrosis Factor-alpha - toxicity |
| title | Priming effects of lipopolysaccharide and inflammatory cytokines on canine granulocytes |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-22T23%3A05%3A32IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Priming%20effects%20of%20lipopolysaccharide%20and%20inflammatory%20cytokines%20on%20canine%20granulocytes&rft.jtitle=Journal%20of%20Veterinary%20Medical%20Science&rft.au=Maeda,%20K.,%20Kitasato%20Univ.,%20Towada,%20Aomori%20(Japan).%20School%20of%20Veterinary%20Medicine&rft.date=2010-01-01&rft.volume=72&rft.issue=1&rft.spage=55&rft.epage=60&rft.pages=55-60&rft.issn=0916-7250&rft.eissn=1347-7439&rft_id=info:doi/10.1292/jvms.08-0494&rft_dat=%3Cproquest_cross%3E3158676781%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1468720174&rft_id=info:pmid/19915328&rfr_iscdi=true |