Protein expression profiling of primary mammary epithelial cells derived from MMTV‐neu mice revealed that HER2/NEU‐driven changes in protein expression are functionally clustered
MMTV‐neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old M...
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| Veröffentlicht in: | IUBMB life 2010-01, Vol.62 (1), p.41-50 |
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| creator | Park, Sungwoo Lee, Kyung‐min Ju, Ji‐hyun Kim, Jaeyoon Noh, Dong‐Young Lee, Taehoon Shin, Incheol |
| description | MMTV‐neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV‐neu heterozygote mice and wild type (WT) littermates using two‐dimensional digest (ChemDigest™/Trypsin)‐LC‐MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti‐apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion‐related proteins were downregulated in PMECs from MMTV‐neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU‐driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010 |
| doi_str_mv | 10.1002/iub.276 |
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To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV‐neu heterozygote mice and wild type (WT) littermates using two‐dimensional digest (ChemDigest™/Trypsin)‐LC‐MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti‐apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion‐related proteins were downregulated in PMECs from MMTV‐neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU‐driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010</description><identifier>ISSN: 1521-6543</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1002/iub.276</identifier><identifier>PMID: 19960538</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., a Wiley company</publisher><subject>Animals ; Apoptosis ; Blotting, Western ; Cell Adhesion ; Cell Line, Tumor ; Epithelial Cells - metabolism ; Gene Expression Regulation ; Genotype ; HER2/NEU ; Humans ; Mammary Glands, Human - cytology ; Mammary Glands, Human - metabolism ; Mammary Tumor Virus, Mouse ; Mass Spectrometry ; Mice ; Mice, Transgenic ; Protein Array Analysis ; Proteomics ; proteonomics ; Receptor, ErbB-2 - metabolism ; signal transduction</subject><ispartof>IUBMB life, 2010-01, Vol.62 (1), p.41-50</ispartof><rights>Copyright © 2009 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3166-26643d4042df4dbb4a9b3453305156c7494ad57fe9c29dedf427603c79a6031d3</citedby><cites>FETCH-LOGICAL-c3166-26643d4042df4dbb4a9b3453305156c7494ad57fe9c29dedf427603c79a6031d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fiub.276$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fiub.276$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19960538$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Sungwoo</creatorcontrib><creatorcontrib>Lee, Kyung‐min</creatorcontrib><creatorcontrib>Ju, Ji‐hyun</creatorcontrib><creatorcontrib>Kim, Jaeyoon</creatorcontrib><creatorcontrib>Noh, Dong‐Young</creatorcontrib><creatorcontrib>Lee, Taehoon</creatorcontrib><creatorcontrib>Shin, Incheol</creatorcontrib><title>Protein expression profiling of primary mammary epithelial cells derived from MMTV‐neu mice revealed that HER2/NEU‐driven changes in protein expression are functionally clustered</title><title>IUBMB life</title><addtitle>IUBMB Life</addtitle><description>MMTV‐neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV‐neu heterozygote mice and wild type (WT) littermates using two‐dimensional digest (ChemDigest™/Trypsin)‐LC‐MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti‐apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion‐related proteins were downregulated in PMECs from MMTV‐neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU‐driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Blotting, Western</subject><subject>Cell Adhesion</subject><subject>Cell Line, Tumor</subject><subject>Epithelial Cells - metabolism</subject><subject>Gene Expression Regulation</subject><subject>Genotype</subject><subject>HER2/NEU</subject><subject>Humans</subject><subject>Mammary Glands, Human - cytology</subject><subject>Mammary Glands, Human - metabolism</subject><subject>Mammary Tumor Virus, Mouse</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>Protein Array Analysis</subject><subject>Proteomics</subject><subject>proteonomics</subject><subject>Receptor, ErbB-2 - metabolism</subject><subject>signal transduction</subject><issn>1521-6543</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kctOxCAUhonReI9vYNi5MDNCoXRYqhkvyXiJcdw2FE4dDG1HaNXZ-Qg-jQ_kk8joRBMT2fyc8PFzOD9CO5T0KSHJge2KfpKJJbRO04T2RJrS5Z89Z2toI4QHEldG5Cpao1IKkrLBOnq_9k0LtsbwMvUQgm1qPPVNaZ2t73FTxsJWys9wpaovhaltJ-CscliDcwEb8PYJDC59U-GLi9u7j9e3GjpcWQ3YwxMoF0_biWrx2fAmObgcjiNh5pdqrCeqvoeA7derfxtRHnDZ1bqNhXJuhrXrQgsezBZaKZULsL3QTTQ-Gd4en_VGV6fnx4ejnmZUiF4iBGeGE56Ykpui4EoWjKeMkZSmQmdccmXSrASpE2kgQnGIhOlMqijUsE209-0bu3vsILR5ZcP836qGpgt5xthASDIgv6T2TQgeynwxuZySfJ5RHjPKo30kdxeeXVGB-eUWoURg_xt4tg5m__nk5-Ojud0nlYCf-w</recordid><startdate>201001</startdate><enddate>201001</enddate><creator>Park, Sungwoo</creator><creator>Lee, Kyung‐min</creator><creator>Ju, Ji‐hyun</creator><creator>Kim, Jaeyoon</creator><creator>Noh, Dong‐Young</creator><creator>Lee, Taehoon</creator><creator>Shin, Incheol</creator><general>Wiley Subscription Services, Inc., a Wiley company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201001</creationdate><title>Protein expression profiling of primary mammary epithelial cells derived from MMTV‐neu mice revealed that HER2/NEU‐driven changes in protein expression are functionally clustered</title><author>Park, Sungwoo ; Lee, Kyung‐min ; Ju, Ji‐hyun ; Kim, Jaeyoon ; Noh, Dong‐Young ; Lee, Taehoon ; Shin, Incheol</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3166-26643d4042df4dbb4a9b3453305156c7494ad57fe9c29dedf427603c79a6031d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Blotting, Western</topic><topic>Cell Adhesion</topic><topic>Cell Line, Tumor</topic><topic>Epithelial Cells - metabolism</topic><topic>Gene Expression Regulation</topic><topic>Genotype</topic><topic>HER2/NEU</topic><topic>Humans</topic><topic>Mammary Glands, Human - cytology</topic><topic>Mammary Glands, Human - metabolism</topic><topic>Mammary Tumor Virus, Mouse</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>Protein Array Analysis</topic><topic>Proteomics</topic><topic>proteonomics</topic><topic>Receptor, ErbB-2 - metabolism</topic><topic>signal transduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Park, Sungwoo</creatorcontrib><creatorcontrib>Lee, Kyung‐min</creatorcontrib><creatorcontrib>Ju, Ji‐hyun</creatorcontrib><creatorcontrib>Kim, Jaeyoon</creatorcontrib><creatorcontrib>Noh, Dong‐Young</creatorcontrib><creatorcontrib>Lee, Taehoon</creatorcontrib><creatorcontrib>Shin, Incheol</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>IUBMB life</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Sungwoo</au><au>Lee, Kyung‐min</au><au>Ju, Ji‐hyun</au><au>Kim, Jaeyoon</au><au>Noh, Dong‐Young</au><au>Lee, Taehoon</au><au>Shin, Incheol</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein expression profiling of primary mammary epithelial cells derived from MMTV‐neu mice revealed that HER2/NEU‐driven changes in protein expression are functionally clustered</atitle><jtitle>IUBMB life</jtitle><addtitle>IUBMB Life</addtitle><date>2010-01</date><risdate>2010</risdate><volume>62</volume><issue>1</issue><spage>41</spage><epage>50</epage><pages>41-50</pages><issn>1521-6543</issn><eissn>1521-6551</eissn><abstract>MMTV‐neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV‐neu heterozygote mice and wild type (WT) littermates using two‐dimensional digest (ChemDigest™/Trypsin)‐LC‐MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti‐apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion‐related proteins were downregulated in PMECs from MMTV‐neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU‐driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., a Wiley company</pub><pmid>19960538</pmid><doi>10.1002/iub.276</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Apoptosis Blotting, Western Cell Adhesion Cell Line, Tumor Epithelial Cells - metabolism Gene Expression Regulation Genotype HER2/NEU Humans Mammary Glands, Human - cytology Mammary Glands, Human - metabolism Mammary Tumor Virus, Mouse Mass Spectrometry Mice Mice, Transgenic Protein Array Analysis Proteomics proteonomics Receptor, ErbB-2 - metabolism signal transduction |
| title | Protein expression profiling of primary mammary epithelial cells derived from MMTV‐neu mice revealed that HER2/NEU‐driven changes in protein expression are functionally clustered |
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