Laser Biomodulation on L 929 Cell Culture
Objective: The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm 2 and 50 mJ/cm 2 ) on L929 fibroblast cells. Background: Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important in...
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| Veröffentlicht in: | Photomedicine and laser surgery 2010-04, Vol.28 (2), p.167-171 |
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| container_title | Photomedicine and laser surgery |
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| creator | Pires-Oliveira, Deise. A.A. Oliveira, Rodrigo F. Machado, Aline H.A. Zângaro, Renato A. Pacheco-Soares, Cristina |
| description | Objective:
The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm
2
and 50 mJ/cm
2
) on L929 fibroblast cells.
Background:
Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important
in vitro
photobiomodulation on cell cultures and tissues.
Methods:
Irradiation was performed for three days at 24-h intervals. After each interval, the cells were stained with MitoTracker Orange™ and DioC6 dyes to assess the photobiomodulatory effects of irradiation on mitochondrial activity and changes in the endoplasmic reticulum. The MTT assay [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide] was used to evaluate cell proliferation.
Results and Conclusions:
The fluorescence microscopy assessment of mitochondria and endoplasmic reticulum in cells irradiated with 6 J/cm
2
and 50 mJ/cm
2
demonstrated intense mitochondrial activity, which was confirmed by DioC6 staining. Reticular activity was observed stemming from increased protein synthesis. Photobiomodulation with 50 mJ/cm
2
was slightly higher than with 6 J/cm
2
, as demonstrated by fluorescence microscopy results. Photobiomodulation was also time-dependent, with better results 72-h after irradiation. |
| doi_str_mv | 10.1089/pho.2008.2269 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_733864982</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A224168441</galeid><sourcerecordid>A224168441</sourcerecordid><originalsourceid>FETCH-LOGICAL-c403t-68b4f9497907b0aa31ac4d2d4582c65c7314549996f1fbabe2811095b7ff23493</originalsourceid><addsrcrecordid>eNqFkEtLxDAURoMozji6dCsFF-KiNa-2yXIsvqDgRtchTRONtM2YtAv_vSlVQRAkgYTLufd-HABOEcwQZPxq9-oyDCHLMC74HlijPC9Tludwf_5TnuYUsRU4CuENQsxLTg7BCkNMMOflGlzWMmifXFvXu3bq5GjdkMRbJxzzpNJdl1RTN05eH4MDI7ugT77eDXi-vXmq7tP68e6h2tapopCMacEaajiNi2DZQCkJkoq2uKU5w6rIVUkQjbE4LwwyjWw0ZghBnjelMZhQTjbgYpm78-590mEUvQ0qBpGDdlMQJSGsoJzhSJ4v5IvstLCDcaOXaqbFFmOKCkYpilT2BxVPq3ur3KCNjfVfDenSoLwLwWsjdt720n8IBMXsXETnYnYuZueRP_sKPDW9bn_ob8kRIAswl-UwdFY32o__jP0EN6CJGQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>733864982</pqid></control><display><type>article</type><title>Laser Biomodulation on L 929 Cell Culture</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Pires-Oliveira, Deise. A.A. ; Oliveira, Rodrigo F. ; Machado, Aline H.A. ; Zângaro, Renato A. ; Pacheco-Soares, Cristina</creator><creatorcontrib>Pires-Oliveira, Deise. A.A. ; Oliveira, Rodrigo F. ; Machado, Aline H.A. ; Zângaro, Renato A. ; Pacheco-Soares, Cristina</creatorcontrib><description>Objective:
The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm
2
and 50 mJ/cm
2
) on L929 fibroblast cells.
Background:
Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important
in vitro
photobiomodulation on cell cultures and tissues.
Methods:
Irradiation was performed for three days at 24-h intervals. After each interval, the cells were stained with MitoTracker Orange™ and DioC6 dyes to assess the photobiomodulatory effects of irradiation on mitochondrial activity and changes in the endoplasmic reticulum. The MTT assay [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide] was used to evaluate cell proliferation.
Results and Conclusions:
The fluorescence microscopy assessment of mitochondria and endoplasmic reticulum in cells irradiated with 6 J/cm
2
and 50 mJ/cm
2
demonstrated intense mitochondrial activity, which was confirmed by DioC6 staining. Reticular activity was observed stemming from increased protein synthesis. Photobiomodulation with 50 mJ/cm
2
was slightly higher than with 6 J/cm
2
, as demonstrated by fluorescence microscopy results. Photobiomodulation was also time-dependent, with better results 72-h after irradiation.</description><identifier>ISSN: 1549-5418</identifier><identifier>EISSN: 1557-8550</identifier><identifier>DOI: 10.1089/pho.2008.2269</identifier><identifier>PMID: 20232997</identifier><language>eng</language><publisher>United States: Mary Ann Liebert, Inc</publisher><subject>Animals ; Cell culture ; Cell Division - radiation effects ; Cells, Cultured ; Endoplasmic Reticulum - radiation effects ; Fibroblasts - radiation effects ; Lasers ; Lasers in medicine ; Mice ; Microscopy, Fluorescence ; Mitochondria - radiation effects ; Original Articles ; Protein biosynthesis ; Tetrazolium Salts ; Time Factors</subject><ispartof>Photomedicine and laser surgery, 2010-04, Vol.28 (2), p.167-171</ispartof><rights>2010, Mary Ann Liebert, Inc.</rights><rights>COPYRIGHT 2010 Mary Ann Liebert, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c403t-68b4f9497907b0aa31ac4d2d4582c65c7314549996f1fbabe2811095b7ff23493</citedby><cites>FETCH-LOGICAL-c403t-68b4f9497907b0aa31ac4d2d4582c65c7314549996f1fbabe2811095b7ff23493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20232997$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pires-Oliveira, Deise. A.A.</creatorcontrib><creatorcontrib>Oliveira, Rodrigo F.</creatorcontrib><creatorcontrib>Machado, Aline H.A.</creatorcontrib><creatorcontrib>Zângaro, Renato A.</creatorcontrib><creatorcontrib>Pacheco-Soares, Cristina</creatorcontrib><title>Laser Biomodulation on L 929 Cell Culture</title><title>Photomedicine and laser surgery</title><addtitle>Photomed Laser Surg</addtitle><description>Objective:
The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm
2
and 50 mJ/cm
2
) on L929 fibroblast cells.
Background:
Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important
in vitro
photobiomodulation on cell cultures and tissues.
Methods:
Irradiation was performed for three days at 24-h intervals. After each interval, the cells were stained with MitoTracker Orange™ and DioC6 dyes to assess the photobiomodulatory effects of irradiation on mitochondrial activity and changes in the endoplasmic reticulum. The MTT assay [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide] was used to evaluate cell proliferation.
Results and Conclusions:
The fluorescence microscopy assessment of mitochondria and endoplasmic reticulum in cells irradiated with 6 J/cm
2
and 50 mJ/cm
2
demonstrated intense mitochondrial activity, which was confirmed by DioC6 staining. Reticular activity was observed stemming from increased protein synthesis. Photobiomodulation with 50 mJ/cm
2
was slightly higher than with 6 J/cm
2
, as demonstrated by fluorescence microscopy results. Photobiomodulation was also time-dependent, with better results 72-h after irradiation.</description><subject>Animals</subject><subject>Cell culture</subject><subject>Cell Division - radiation effects</subject><subject>Cells, Cultured</subject><subject>Endoplasmic Reticulum - radiation effects</subject><subject>Fibroblasts - radiation effects</subject><subject>Lasers</subject><subject>Lasers in medicine</subject><subject>Mice</subject><subject>Microscopy, Fluorescence</subject><subject>Mitochondria - radiation effects</subject><subject>Original Articles</subject><subject>Protein biosynthesis</subject><subject>Tetrazolium Salts</subject><subject>Time Factors</subject><issn>1549-5418</issn><issn>1557-8550</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtLxDAURoMozji6dCsFF-KiNa-2yXIsvqDgRtchTRONtM2YtAv_vSlVQRAkgYTLufd-HABOEcwQZPxq9-oyDCHLMC74HlijPC9Tludwf_5TnuYUsRU4CuENQsxLTg7BCkNMMOflGlzWMmifXFvXu3bq5GjdkMRbJxzzpNJdl1RTN05eH4MDI7ugT77eDXi-vXmq7tP68e6h2tapopCMacEaajiNi2DZQCkJkoq2uKU5w6rIVUkQjbE4LwwyjWw0ZghBnjelMZhQTjbgYpm78-590mEUvQ0qBpGDdlMQJSGsoJzhSJ4v5IvstLCDcaOXaqbFFmOKCkYpilT2BxVPq3ur3KCNjfVfDenSoLwLwWsjdt720n8IBMXsXETnYnYuZueRP_sKPDW9bn_ob8kRIAswl-UwdFY32o__jP0EN6CJGQ</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Pires-Oliveira, Deise. A.A.</creator><creator>Oliveira, Rodrigo F.</creator><creator>Machado, Aline H.A.</creator><creator>Zângaro, Renato A.</creator><creator>Pacheco-Soares, Cristina</creator><general>Mary Ann Liebert, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100401</creationdate><title>Laser Biomodulation on L 929 Cell Culture</title><author>Pires-Oliveira, Deise. A.A. ; Oliveira, Rodrigo F. ; Machado, Aline H.A. ; Zângaro, Renato A. ; Pacheco-Soares, Cristina</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c403t-68b4f9497907b0aa31ac4d2d4582c65c7314549996f1fbabe2811095b7ff23493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Cell culture</topic><topic>Cell Division - radiation effects</topic><topic>Cells, Cultured</topic><topic>Endoplasmic Reticulum - radiation effects</topic><topic>Fibroblasts - radiation effects</topic><topic>Lasers</topic><topic>Lasers in medicine</topic><topic>Mice</topic><topic>Microscopy, Fluorescence</topic><topic>Mitochondria - radiation effects</topic><topic>Original Articles</topic><topic>Protein biosynthesis</topic><topic>Tetrazolium Salts</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pires-Oliveira, Deise. A.A.</creatorcontrib><creatorcontrib>Oliveira, Rodrigo F.</creatorcontrib><creatorcontrib>Machado, Aline H.A.</creatorcontrib><creatorcontrib>Zângaro, Renato A.</creatorcontrib><creatorcontrib>Pacheco-Soares, Cristina</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Photomedicine and laser surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pires-Oliveira, Deise. A.A.</au><au>Oliveira, Rodrigo F.</au><au>Machado, Aline H.A.</au><au>Zângaro, Renato A.</au><au>Pacheco-Soares, Cristina</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Laser Biomodulation on L 929 Cell Culture</atitle><jtitle>Photomedicine and laser surgery</jtitle><addtitle>Photomed Laser Surg</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>28</volume><issue>2</issue><spage>167</spage><epage>171</epage><pages>167-171</pages><issn>1549-5418</issn><eissn>1557-8550</eissn><abstract>Objective:
The aim of the present study was to analyze the effects of photobiomodulation using a 904-nm diode laser at two energy densities (6 J/cm
2
and 50 mJ/cm
2
) on L929 fibroblast cells.
Background:
Low-power laser irradiation (LPLI) is a non-pharmacological resource that induces important
in vitro
photobiomodulation on cell cultures and tissues.
Methods:
Irradiation was performed for three days at 24-h intervals. After each interval, the cells were stained with MitoTracker Orange™ and DioC6 dyes to assess the photobiomodulatory effects of irradiation on mitochondrial activity and changes in the endoplasmic reticulum. The MTT assay [3-(4.5-dimethylthiazol-2-yl)-2.5 diphenyltetrazolium bromide] was used to evaluate cell proliferation.
Results and Conclusions:
The fluorescence microscopy assessment of mitochondria and endoplasmic reticulum in cells irradiated with 6 J/cm
2
and 50 mJ/cm
2
demonstrated intense mitochondrial activity, which was confirmed by DioC6 staining. Reticular activity was observed stemming from increased protein synthesis. Photobiomodulation with 50 mJ/cm
2
was slightly higher than with 6 J/cm
2
, as demonstrated by fluorescence microscopy results. Photobiomodulation was also time-dependent, with better results 72-h after irradiation.</abstract><cop>United States</cop><pub>Mary Ann Liebert, Inc</pub><pmid>20232997</pmid><doi>10.1089/pho.2008.2269</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1549-5418 |
| ispartof | Photomedicine and laser surgery, 2010-04, Vol.28 (2), p.167-171 |
| issn | 1549-5418 1557-8550 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733864982 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cell culture Cell Division - radiation effects Cells, Cultured Endoplasmic Reticulum - radiation effects Fibroblasts - radiation effects Lasers Lasers in medicine Mice Microscopy, Fluorescence Mitochondria - radiation effects Original Articles Protein biosynthesis Tetrazolium Salts Time Factors |
| title | Laser Biomodulation on L 929 Cell Culture |
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