Chemically Induced Cardiomyogenesis of Mouse Embryonic Stem Cells
A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of α-myosine heavy chain (α-MHC) promoter (pα-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening o...
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| Veröffentlicht in: | Chembiochem : a European journal of chemical biology 2010-01, Vol.11 (2), p.208-217 |
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| container_title | Chembiochem : a European journal of chemical biology |
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| creator | Berkessel, Albrecht Seelig, Bianca Schwengberg, Silke Hescheler, Jürgen Sachinidis, Agapios |
| description | A transgenic murine embryonic stem (ES) cell lineage expressing enhanced green fluorescent protein (EGFP) under the control of α-myosine heavy chain (α-MHC) promoter (pα-MHC-EGFP) was used to investigate the effects of (thio)urea and cinchona alkaloid derivatives on cardiomyogenesis. The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80 % increase in the EGFP fluorescence compared to untreated cells. Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellular targets. |
| doi_str_mv | 10.1002/cbic.200900345 |
| format | Article |
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The screening of the compounds yielded cardiomyogenesis inducing substances with good (IV-5, V-4) to very good activities (II-16, IV-8), as determined by a 50 to 80 % increase in the EGFP fluorescence compared to untreated cells. 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Time-dependent screening approaches in which compounds were added at different developmental stages of the ES cells appeared to be of limited suitability for the identification of potential cellular targets.</description><subject>Animals</subject><subject>cardiomyogenesis</subject><subject>Cell Differentiation</subject><subject>cells</subject><subject>cinchona alkaloids</subject><subject>Cinchona Alkaloids - chemistry</subject><subject>Cinchona Alkaloids - pharmacology</subject><subject>Drug Design</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Embryonic Stem Cells - metabolism</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Mice</subject><subject>Myocytes, Cardiac - cytology</subject><subject>Myocytes, Cardiac - metabolism</subject><subject>screening</subject><subject>Signal Transduction</subject><subject>stem cells</subject><subject>Thiourea - chemistry</subject><subject>Thiourea - pharmacology</subject><subject>Time Factors</subject><issn>1439-4227</issn><issn>1439-7633</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkD1v2zAQhomiRT6crBlTbZ3kkjyJNMdUyIfRJAWaBAG6ECR1StlKlkvaSPTvQ0OukS3T3fA8L-5eQk4YnTJK-VdnvZtyShWlUJQfyAErQOVSAHzc7gXncp8cxviHJkwA2yP7SQDFS3ZAzqrf2Hln2nbI5ot67bDOKhNq33dD_4QLjD5mfZPd9OuI2Xlnw9AvvMvuVthlFbZtPCKfGtNGPN7OCXm4OL-vrvLrH5fz6uw6dwUVZd4YAwY4KyxASRlK0YgCKTc1kxy5UEKUplB2Jm0jrSqxBFtza7hTloNiMCFfxtxl6P-tMa5056NLF5gFpuO0BJilt0ElcjqSLvQxBmz0MvjOhEEzqjet6U1retdaEk630WvbYb3D_9eUADUCz77F4Z04XX2bV2_D89H1cYUvO9eEv1pIkKV-vL3UP7_fXF384rf6MfGfR74xvTZPwUf9cMcpA8qkmhWMwStKmJA0</recordid><startdate>20100125</startdate><enddate>20100125</enddate><creator>Berkessel, Albrecht</creator><creator>Seelig, Bianca</creator><creator>Schwengberg, Silke</creator><creator>Hescheler, Jürgen</creator><creator>Sachinidis, Agapios</creator><general>Wiley-VCH Verlag</general><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100125</creationdate><title>Chemically Induced Cardiomyogenesis of Mouse Embryonic Stem Cells</title><author>Berkessel, Albrecht ; 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| subjects | Animals cardiomyogenesis Cell Differentiation cells cinchona alkaloids Cinchona Alkaloids - chemistry Cinchona Alkaloids - pharmacology Drug Design Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Green Fluorescent Proteins - metabolism Mice Myocytes, Cardiac - cytology Myocytes, Cardiac - metabolism screening Signal Transduction stem cells Thiourea - chemistry Thiourea - pharmacology Time Factors |
| title | Chemically Induced Cardiomyogenesis of Mouse Embryonic Stem Cells |
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