A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer
A solid-state electrochemiluminescence sensing platform based on ferrocene-labeled structure-switching signaling aptamer (Fc-aptamer) for highly sensitive detection of small molecules is developed successfully using adenosine as a model analyte. Such special sensing platform included two main parts,...
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| Veröffentlicht in: | Analytica chimica acta 2010-01, Vol.658 (2), p.128-132 |
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| creator | Wang, Xiaoying Dong, Ping He, Pingang Fang, Yuzhi |
| description | A solid-state electrochemiluminescence sensing platform based on ferrocene-labeled structure-switching signaling aptamer (Fc-aptamer) for highly sensitive detection of small molecules is developed successfully using adenosine as a model analyte. Such special sensing platform included two main parts, an electrochemiluminescence (ECL) substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)
3
2+–AuNPs) onto Au electrode. An anti-adenosine aptamer labeled by ferrocene acted as the ECL intensity switch. A short complementary ssDNA for the aptamer was applied to hybridizing with the aptamer, yielding a double-stranded complex of the aptamer and the ssDNA on the electrode surface. The introduction of adenosine triggered structure switching of the aptamer. As a result, the ssDNA was forced to dissociate from the sensing platform. Such structural change of the aptamer resulted in an obvious ECL intensity decrease due to the increased quenching effect of Fc to the ECL substrate. The analytic results were sensitive and specific. |
| doi_str_mv | 10.1016/j.aca.2009.11.007 |
| format | Article |
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3
2+–AuNPs) onto Au electrode. An anti-adenosine aptamer labeled by ferrocene acted as the ECL intensity switch. A short complementary ssDNA for the aptamer was applied to hybridizing with the aptamer, yielding a double-stranded complex of the aptamer and the ssDNA on the electrode surface. The introduction of adenosine triggered structure switching of the aptamer. As a result, the ssDNA was forced to dissociate from the sensing platform. Such structural change of the aptamer resulted in an obvious ECL intensity decrease due to the increased quenching effect of Fc to the ECL substrate. The analytic results were sensitive and specific.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2009.11.007</identifier><identifier>PMID: 20103085</identifier><identifier>CODEN: ACACAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Adenosine ; Adenosine - analysis ; Anti-adenosine aptamer ; Aptamers, Nucleotide - chemistry ; Biphenyl Compounds - chemistry ; Chemistry ; DNA, Single-Stranded - chemistry ; Electrochemical Techniques - methods ; Electrochemistry ; Electrodes ; Exact sciences and technology ; Ferrocene ; Ferrous Compounds - chemistry ; General and physical chemistry ; Gold - chemistry ; Luminescent Measurements - methods ; Metal Nanoparticles - chemistry ; Metallocenes ; Photoelectrochemistry. Electrochemiluminescence ; Quenching effect ; Ruthenium - chemistry ; Solid-state electrochemiluminescence sensing platform</subject><ispartof>Analytica chimica acta, 2010-01, Vol.658 (2), p.128-132</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-5a58d96923ebcb3a18975d296268bd25e5f4178d398e75eebb5358903d81985a3</citedby><cites>FETCH-LOGICAL-c413t-5a58d96923ebcb3a18975d296268bd25e5f4178d398e75eebb5358903d81985a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2009.11.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22289431$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20103085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Xiaoying</creatorcontrib><creatorcontrib>Dong, Ping</creatorcontrib><creatorcontrib>He, Pingang</creatorcontrib><creatorcontrib>Fang, Yuzhi</creatorcontrib><title>A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>A solid-state electrochemiluminescence sensing platform based on ferrocene-labeled structure-switching signaling aptamer (Fc-aptamer) for highly sensitive detection of small molecules is developed successfully using adenosine as a model analyte. Such special sensing platform included two main parts, an electrochemiluminescence (ECL) substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)
3
2+–AuNPs) onto Au electrode. An anti-adenosine aptamer labeled by ferrocene acted as the ECL intensity switch. A short complementary ssDNA for the aptamer was applied to hybridizing with the aptamer, yielding a double-stranded complex of the aptamer and the ssDNA on the electrode surface. The introduction of adenosine triggered structure switching of the aptamer. As a result, the ssDNA was forced to dissociate from the sensing platform. Such structural change of the aptamer resulted in an obvious ECL intensity decrease due to the increased quenching effect of Fc to the ECL substrate. The analytic results were sensitive and specific.</description><subject>Adenosine</subject><subject>Adenosine - analysis</subject><subject>Anti-adenosine aptamer</subject><subject>Aptamers, Nucleotide - chemistry</subject><subject>Biphenyl Compounds - chemistry</subject><subject>Chemistry</subject><subject>DNA, Single-Stranded - chemistry</subject><subject>Electrochemical Techniques - methods</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Exact sciences and technology</subject><subject>Ferrocene</subject><subject>Ferrous Compounds - chemistry</subject><subject>General and physical chemistry</subject><subject>Gold - chemistry</subject><subject>Luminescent Measurements - methods</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Metallocenes</subject><subject>Photoelectrochemistry. Electrochemiluminescence</subject><subject>Quenching effect</subject><subject>Ruthenium - chemistry</subject><subject>Solid-state electrochemiluminescence sensing platform</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctu1DAUhi0EokPhAdggb4BVgi9x4ohVVbWAVKmbsrYc-6T1yLEH26HiOXhhHM0Au25803d--ZwPobeUtJTQ_tO-1Ua3jJCxpbQlZHiGdlQOvOk4656jHSGEN6wfyBl6lfO-Xhkl3Ut0xgglnEixQ78vcI7e2SYXXQCDB1NSNA-wOL8uLkA2EAzgDCG7cI8PXpc5pgXXBVsoFXcx4DhjbSHEygCedAaL6-sMqWZBgMbrqUZbnEtaTVkTNPnRFfOwRWZ3H7TfTvpQ9ALpNXoxa5_hzWk_R9-vr-4uvzY3t1--XV7cNKajvDRCC2nHfmQcJjNxTeU4CMvGnvVyskyAmDs6SMtHCYMAmCbBhRwJt5KOUmh-jj4ecw8p_lghF7W42q73OkBcsxo4lzVM8Ep-eJLkPRdD1_cVpEfQpJhzglkdklt0-qUoUZsztVfVmdqcKUpVdVZr3p3C12kB-6_ir6QKvD8BOhvt56SDcfk_x5gcO04r9_nIQR3aTwdJZeM2e9al6knZ6J74xh9QBrbc</recordid><startdate>20100125</startdate><enddate>20100125</enddate><creator>Wang, Xiaoying</creator><creator>Dong, Ping</creator><creator>He, Pingang</creator><creator>Fang, Yuzhi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U5</scope><scope>8FD</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20100125</creationdate><title>A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer</title><author>Wang, Xiaoying ; Dong, Ping ; He, Pingang ; Fang, Yuzhi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-5a58d96923ebcb3a18975d296268bd25e5f4178d398e75eebb5358903d81985a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adenosine</topic><topic>Adenosine - analysis</topic><topic>Anti-adenosine aptamer</topic><topic>Aptamers, Nucleotide - chemistry</topic><topic>Biphenyl Compounds - chemistry</topic><topic>Chemistry</topic><topic>DNA, Single-Stranded - chemistry</topic><topic>Electrochemical Techniques - methods</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Exact sciences and technology</topic><topic>Ferrocene</topic><topic>Ferrous Compounds - chemistry</topic><topic>General and physical chemistry</topic><topic>Gold - chemistry</topic><topic>Luminescent Measurements - methods</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Metallocenes</topic><topic>Photoelectrochemistry. Electrochemiluminescence</topic><topic>Quenching effect</topic><topic>Ruthenium - chemistry</topic><topic>Solid-state electrochemiluminescence sensing platform</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Xiaoying</creatorcontrib><creatorcontrib>Dong, Ping</creatorcontrib><creatorcontrib>He, Pingang</creatorcontrib><creatorcontrib>Fang, Yuzhi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Xiaoying</au><au>Dong, Ping</au><au>He, Pingang</au><au>Fang, Yuzhi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2010-01-25</date><risdate>2010</risdate><volume>658</volume><issue>2</issue><spage>128</spage><epage>132</epage><pages>128-132</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><coden>ACACAM</coden><abstract>A solid-state electrochemiluminescence sensing platform based on ferrocene-labeled structure-switching signaling aptamer (Fc-aptamer) for highly sensitive detection of small molecules is developed successfully using adenosine as a model analyte. Such special sensing platform included two main parts, an electrochemiluminescence (ECL) substrate and an ECL intensity switch. The ECL substrate was made by modifying the complex of Au nanoparticle and Ruthenium (II) tris-(bipyridine) (Ru(bpy)
3
2+–AuNPs) onto Au electrode. An anti-adenosine aptamer labeled by ferrocene acted as the ECL intensity switch. A short complementary ssDNA for the aptamer was applied to hybridizing with the aptamer, yielding a double-stranded complex of the aptamer and the ssDNA on the electrode surface. The introduction of adenosine triggered structure switching of the aptamer. As a result, the ssDNA was forced to dissociate from the sensing platform. Such structural change of the aptamer resulted in an obvious ECL intensity decrease due to the increased quenching effect of Fc to the ECL substrate. The analytic results were sensitive and specific.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>20103085</pmid><doi>10.1016/j.aca.2009.11.007</doi><tpages>5</tpages></addata></record> |
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| subjects | Adenosine Adenosine - analysis Anti-adenosine aptamer Aptamers, Nucleotide - chemistry Biphenyl Compounds - chemistry Chemistry DNA, Single-Stranded - chemistry Electrochemical Techniques - methods Electrochemistry Electrodes Exact sciences and technology Ferrocene Ferrous Compounds - chemistry General and physical chemistry Gold - chemistry Luminescent Measurements - methods Metal Nanoparticles - chemistry Metallocenes Photoelectrochemistry. Electrochemiluminescence Quenching effect Ruthenium - chemistry Solid-state electrochemiluminescence sensing platform |
| title | A solid-state electrochemiluminescence sensing platform for detection of adenosine based on ferrocene-labeled structure-switching signaling aptamer |
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