Effects of a Novel Pathogenesis-Related Class 10 (PR-10) Protein from Crotalaria pallida Roots with Papain Inhibitory Activity against Root-Knot Nematode Meloidogyne incognita
A novel pathogenesis-related class 10 (PR-10) protein with papain inhibitory activity, named CpPRI, was purified from Crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (HPLCs). CpPRI is made up of a single polypeptide...
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| Veröffentlicht in: | Journal of agricultural and food chemistry 2010-04, Vol.58 (7), p.4145-4152 |
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| container_title | Journal of agricultural and food chemistry |
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| creator | Andrade, Lúcia Betânia da S Oliveira, Adeliana S Ribeiro, Jannison K. C Kiyota, Sumika Vasconcelos, Ilka Maria de Oliveira, José Tadeu Abreu de Sales, Maurício P |
| description | A novel pathogenesis-related class 10 (PR-10) protein with papain inhibitory activity, named CpPRI, was purified from Crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (HPLCs). CpPRI is made up of a single polypeptide chain with a M r of 15 kDa, as determined by sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE). This protein exhibited a K i value of 1.8 × 10−9 M and operates via a noncompetitive inhibition mechanism. The alignment of the N-terminal amino acid sequence of CpPRI with other proteins revealed its identity with PR-10 proteins. CpPRI acts against digestive proteinase from root-knot nematode Meloidogyne incognita and demonstrated nematostatic and nematicide effects on this parasite in bioassays. In a localization study, fluorescein-5-isothiocyanate (FITC)−CpPRI was observed to internalize and diffuse over the entire J2 body after 6 h of incubation. This fact could explain the natural tolerance of this plant species to nematodes. |
| doi_str_mv | 10.1021/jf9044556 |
| format | Article |
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CpPRI acts against digestive proteinase from root-knot nematode Meloidogyne incognita and demonstrated nematostatic and nematicide effects on this parasite in bioassays. In a localization study, fluorescein-5-isothiocyanate (FITC)−CpPRI was observed to internalize and diffuse over the entire J2 body after 6 h of incubation. This fact could explain the natural tolerance of this plant species to nematodes.</description><identifier>ISSN: 0021-8561</identifier><identifier>EISSN: 1520-5118</identifier><identifier>DOI: 10.1021/jf9044556</identifier><identifier>PMID: 20199085</identifier><identifier>CODEN: JAFCAU</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Animals ; Bioactive Constituents ; Biological and medical sciences ; biological control ; Crotalaria - chemistry ; Crotalaria pallida ; digestive enzymes ; enzyme inhibition ; Enzyme Inhibitors - chemistry ; Enzyme Inhibitors - isolation & purification ; Enzyme Inhibitors - pharmacology ; Food industries ; Fundamental and applied biological sciences. Psychology ; Kinetics ; Lycopersicon esculentum - parasitology ; Meloidogyne incognita ; Molecular Weight ; nematicidal properties ; nematode control ; nematostatic properties ; papain ; Papain - antagonists & inhibitors ; pest resistance ; Plant Diseases - parasitology ; plant extracts ; plant proteins ; Plant Proteins - chemistry ; Plant Proteins - isolation & purification ; Plant Proteins - pharmacology ; Plant Roots - parasitology ; proteinases ; root-knot nematodes ; roots ; Tylenchoidea - drug effects ; Tylenchoidea - physiology</subject><ispartof>Journal of agricultural and food chemistry, 2010-04, Vol.58 (7), p.4145-4152</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a368t-e1428b506d01f68e7db5bf0467607977a5f62a012cc926a93051e99882a76f903</citedby><cites>FETCH-LOGICAL-a368t-e1428b506d01f68e7db5bf0467607977a5f62a012cc926a93051e99882a76f903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/jf9044556$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/jf9044556$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22642292$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20199085$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Andrade, Lúcia Betânia da S</creatorcontrib><creatorcontrib>Oliveira, Adeliana S</creatorcontrib><creatorcontrib>Ribeiro, Jannison K. C</creatorcontrib><creatorcontrib>Kiyota, Sumika</creatorcontrib><creatorcontrib>Vasconcelos, Ilka Maria</creatorcontrib><creatorcontrib>de Oliveira, José Tadeu Abreu</creatorcontrib><creatorcontrib>de Sales, Maurício P</creatorcontrib><title>Effects of a Novel Pathogenesis-Related Class 10 (PR-10) Protein from Crotalaria pallida Roots with Papain Inhibitory Activity against Root-Knot Nematode Meloidogyne incognita</title><title>Journal of agricultural and food chemistry</title><addtitle>J. Agric. Food Chem</addtitle><description>A novel pathogenesis-related class 10 (PR-10) protein with papain inhibitory activity, named CpPRI, was purified from Crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (HPLCs). CpPRI is made up of a single polypeptide chain with a M r of 15 kDa, as determined by sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE). This protein exhibited a K i value of 1.8 × 10−9 M and operates via a noncompetitive inhibition mechanism. The alignment of the N-terminal amino acid sequence of CpPRI with other proteins revealed its identity with PR-10 proteins. CpPRI acts against digestive proteinase from root-knot nematode Meloidogyne incognita and demonstrated nematostatic and nematicide effects on this parasite in bioassays. In a localization study, fluorescein-5-isothiocyanate (FITC)−CpPRI was observed to internalize and diffuse over the entire J2 body after 6 h of incubation. This fact could explain the natural tolerance of this plant species to nematodes.</description><subject>Animals</subject><subject>Bioactive Constituents</subject><subject>Biological and medical sciences</subject><subject>biological control</subject><subject>Crotalaria - chemistry</subject><subject>Crotalaria pallida</subject><subject>digestive enzymes</subject><subject>enzyme inhibition</subject><subject>Enzyme Inhibitors - chemistry</subject><subject>Enzyme Inhibitors - isolation & purification</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Kinetics</subject><subject>Lycopersicon esculentum - parasitology</subject><subject>Meloidogyne incognita</subject><subject>Molecular Weight</subject><subject>nematicidal properties</subject><subject>nematode control</subject><subject>nematostatic properties</subject><subject>papain</subject><subject>Papain - antagonists & inhibitors</subject><subject>pest resistance</subject><subject>Plant Diseases - parasitology</subject><subject>plant extracts</subject><subject>plant proteins</subject><subject>Plant Proteins - chemistry</subject><subject>Plant Proteins - isolation & purification</subject><subject>Plant Proteins - pharmacology</subject><subject>Plant Roots - parasitology</subject><subject>proteinases</subject><subject>root-knot nematodes</subject><subject>roots</subject><subject>Tylenchoidea - drug effects</subject><subject>Tylenchoidea - physiology</subject><issn>0021-8561</issn><issn>1520-5118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0cFuEzEQBuAVAtFQOPAC4AuCHhbG3rXXPlZRgYpSokDPq9lde-PIWae2U5Sn4hUxJLQXTtZoPv2y5i-KlxTeU2D0w9ooqGvOxaNiRjmDklMqHxczyMtSckFPimcxrgFA8gaeFicMqFJ5mBW_LozRfYrEG4Lk2t9pRxaYVn7Uk442lkvtMOmBzB3GSCiQd4tlSeGMLIJP2k7EBL8h8zygw2CRbNE5OyBZep9jf9q0yoFbzPJyWtnOJh_25LxP9s6mPcExb2L6q8svk0_kWm8w-UGTr9p5O_hxP2lip96Pk034vHhi0EX94vieFjcfL37MP5dX3z5dzs-vSqyETKWmNZMdBzEANULqZuh4Z6AWjYBGNQ1yIxgCZX2vmEBVAadaKSkZNiJfszot3h5yt8Hf7nRM7cbGXjuHk_a72DZVJQVwRbM8O8g--BiDNu022A2GfUuh_VNPe19Ptq-Oqbtuo4d7-a-PDN4cAcYenQk49TY-OCZqxhTL7vXBGfQtjiGbm-85pQIqKyGUekjCPrZrvwtTPtd_vvQboUesRw</recordid><startdate>20100414</startdate><enddate>20100414</enddate><creator>Andrade, Lúcia Betânia da S</creator><creator>Oliveira, Adeliana S</creator><creator>Ribeiro, Jannison K. C</creator><creator>Kiyota, Sumika</creator><creator>Vasconcelos, Ilka Maria</creator><creator>de Oliveira, José Tadeu Abreu</creator><creator>de Sales, Maurício P</creator><general>American Chemical Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100414</creationdate><title>Effects of a Novel Pathogenesis-Related Class 10 (PR-10) Protein from Crotalaria pallida Roots with Papain Inhibitory Activity against Root-Knot Nematode Meloidogyne incognita</title><author>Andrade, Lúcia Betânia da S ; Oliveira, Adeliana S ; Ribeiro, Jannison K. C ; Kiyota, Sumika ; Vasconcelos, Ilka Maria ; de Oliveira, José Tadeu Abreu ; de Sales, Maurício P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a368t-e1428b506d01f68e7db5bf0467607977a5f62a012cc926a93051e99882a76f903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Bioactive Constituents</topic><topic>Biological and medical sciences</topic><topic>biological control</topic><topic>Crotalaria - chemistry</topic><topic>Crotalaria pallida</topic><topic>digestive enzymes</topic><topic>enzyme inhibition</topic><topic>Enzyme Inhibitors - chemistry</topic><topic>Enzyme Inhibitors - isolation & purification</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Kinetics</topic><topic>Lycopersicon esculentum - parasitology</topic><topic>Meloidogyne incognita</topic><topic>Molecular Weight</topic><topic>nematicidal properties</topic><topic>nematode control</topic><topic>nematostatic properties</topic><topic>papain</topic><topic>Papain - antagonists & inhibitors</topic><topic>pest resistance</topic><topic>Plant Diseases - parasitology</topic><topic>plant extracts</topic><topic>plant proteins</topic><topic>Plant Proteins - chemistry</topic><topic>Plant Proteins - isolation & purification</topic><topic>Plant Proteins - pharmacology</topic><topic>Plant Roots - parasitology</topic><topic>proteinases</topic><topic>root-knot nematodes</topic><topic>roots</topic><topic>Tylenchoidea - drug effects</topic><topic>Tylenchoidea - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Andrade, Lúcia Betânia da S</creatorcontrib><creatorcontrib>Oliveira, Adeliana S</creatorcontrib><creatorcontrib>Ribeiro, Jannison K. C</creatorcontrib><creatorcontrib>Kiyota, Sumika</creatorcontrib><creatorcontrib>Vasconcelos, Ilka Maria</creatorcontrib><creatorcontrib>de Oliveira, José Tadeu Abreu</creatorcontrib><creatorcontrib>de Sales, Maurício P</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of agricultural and food chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Andrade, Lúcia Betânia da S</au><au>Oliveira, Adeliana S</au><au>Ribeiro, Jannison K. C</au><au>Kiyota, Sumika</au><au>Vasconcelos, Ilka Maria</au><au>de Oliveira, José Tadeu Abreu</au><au>de Sales, Maurício P</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of a Novel Pathogenesis-Related Class 10 (PR-10) Protein from Crotalaria pallida Roots with Papain Inhibitory Activity against Root-Knot Nematode Meloidogyne incognita</atitle><jtitle>Journal of agricultural and food chemistry</jtitle><addtitle>J. Agric. Food Chem</addtitle><date>2010-04-14</date><risdate>2010</risdate><volume>58</volume><issue>7</issue><spage>4145</spage><epage>4152</epage><pages>4145-4152</pages><issn>0021-8561</issn><eissn>1520-5118</eissn><coden>JAFCAU</coden><abstract>A novel pathogenesis-related class 10 (PR-10) protein with papain inhibitory activity, named CpPRI, was purified from Crotalaria pallida roots by ammonium sulfate precipitation followed by three reverse-phase high-performance liquid chromatographies (HPLCs). CpPRI is made up of a single polypeptide chain with a M r of 15 kDa, as determined by sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE). This protein exhibited a K i value of 1.8 × 10−9 M and operates via a noncompetitive inhibition mechanism. The alignment of the N-terminal amino acid sequence of CpPRI with other proteins revealed its identity with PR-10 proteins. CpPRI acts against digestive proteinase from root-knot nematode Meloidogyne incognita and demonstrated nematostatic and nematicide effects on this parasite in bioassays. In a localization study, fluorescein-5-isothiocyanate (FITC)−CpPRI was observed to internalize and diffuse over the entire J2 body after 6 h of incubation. This fact could explain the natural tolerance of this plant species to nematodes.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20199085</pmid><doi>10.1021/jf9044556</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of agricultural and food chemistry, 2010-04, Vol.58 (7), p.4145-4152 |
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| subjects | Animals Bioactive Constituents Biological and medical sciences biological control Crotalaria - chemistry Crotalaria pallida digestive enzymes enzyme inhibition Enzyme Inhibitors - chemistry Enzyme Inhibitors - isolation & purification Enzyme Inhibitors - pharmacology Food industries Fundamental and applied biological sciences. Psychology Kinetics Lycopersicon esculentum - parasitology Meloidogyne incognita Molecular Weight nematicidal properties nematode control nematostatic properties papain Papain - antagonists & inhibitors pest resistance Plant Diseases - parasitology plant extracts plant proteins Plant Proteins - chemistry Plant Proteins - isolation & purification Plant Proteins - pharmacology Plant Roots - parasitology proteinases root-knot nematodes roots Tylenchoidea - drug effects Tylenchoidea - physiology |
| title | Effects of a Novel Pathogenesis-Related Class 10 (PR-10) Protein from Crotalaria pallida Roots with Papain Inhibitory Activity against Root-Knot Nematode Meloidogyne incognita |
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