SiRNA-mediated IGF-1R inhibition sensitizes human colon cancer SW480 cells to radiation
Abstract Purpose. Insulin like growth factor receptor 1 (IGF-1R) is well-documented to play a key role in radiation response and tumor radiosensitivity, thus offering an attractive clinic drug target to enhance tumor sensitivity to anti-cancer radiotherapy. Material and methods. Human colon carcinom...
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| Veröffentlicht in: | Acta oncologica 2010, Vol.49 (1), p.70-75 |
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| creator | Yavari, Kamal Taghikhani, Mohammad Maragheh, Mohammad Ghannadi Mesbah-Namin, Seyed A. Babaei, Mohammad Hosein Arfaee, Ali Jabbary Madani, Hossein Mirzaei, Hamid Reza |
| description | Abstract
Purpose. Insulin like growth factor receptor 1 (IGF-1R) is well-documented to play a key role in radiation response and tumor radiosensitivity, thus offering an attractive clinic drug target to enhance tumor sensitivity to anti-cancer radiotherapy. Material and methods. Human colon carcinoma SW480 cells were transfected with the specific small interference RNA (siRNA) expression vector (pkD-shRNA-IGF-1R-V2) designed to target IGF-1R mRNA. The expression of IGF-1R mRNA and its protein among the transfected and untransfected cells were detected by semi-quantitative RT-PCR and ELISA assay. The changes in cell radiosensitivity were examined by MTT assay. Results. Transfection of mammalian expression vector pkD containing IGF-1R siRNA was shown to reduce IGF-1R mRNA levels by up to 95%. ELISA assay detected a similar inhibition of IGF-1R protein levels in cells transfected with IGF-1R siRNA. SW480 cells transfected with the expression vector for siRNA significantly rendered cells more sensitive to radiation and the highest radiation enhancement ratio was 2.02 ± 0.08. Conclusion. These data provide the first evidence that specific siRNA fragment (pkD-shRNA-IGF-1R-V2) targeting human IGF-1R mRNA is able to enhance colon cancer radiosensitivity. Also results indicated that, combining IGF-1R siRNA and radiation significantly enhances antitumor efficacy compared with either modality alone. |
| doi_str_mv | 10.3109/02841860903334429 |
| format | Article |
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Purpose. Insulin like growth factor receptor 1 (IGF-1R) is well-documented to play a key role in radiation response and tumor radiosensitivity, thus offering an attractive clinic drug target to enhance tumor sensitivity to anti-cancer radiotherapy. Material and methods. Human colon carcinoma SW480 cells were transfected with the specific small interference RNA (siRNA) expression vector (pkD-shRNA-IGF-1R-V2) designed to target IGF-1R mRNA. The expression of IGF-1R mRNA and its protein among the transfected and untransfected cells were detected by semi-quantitative RT-PCR and ELISA assay. The changes in cell radiosensitivity were examined by MTT assay. Results. Transfection of mammalian expression vector pkD containing IGF-1R siRNA was shown to reduce IGF-1R mRNA levels by up to 95%. ELISA assay detected a similar inhibition of IGF-1R protein levels in cells transfected with IGF-1R siRNA. SW480 cells transfected with the expression vector for siRNA significantly rendered cells more sensitive to radiation and the highest radiation enhancement ratio was 2.02 ± 0.08. Conclusion. These data provide the first evidence that specific siRNA fragment (pkD-shRNA-IGF-1R-V2) targeting human IGF-1R mRNA is able to enhance colon cancer radiosensitivity. Also results indicated that, combining IGF-1R siRNA and radiation significantly enhances antitumor efficacy compared with either modality alone.</description><identifier>ISSN: 0284-186X</identifier><identifier>EISSN: 1651-226X</identifier><identifier>DOI: 10.3109/02841860903334429</identifier><identifier>PMID: 20001499</identifier><language>eng</language><publisher>England: Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS)</publisher><subject>Cell Line, Tumor ; Colonic Neoplasms - genetics ; Enzyme-Linked Immunosorbent Assay ; Humans ; Radiation Tolerance - genetics ; Radiation-Sensitizing Agents - pharmacology ; Receptor, IGF Type 1 - antagonists & inhibitors ; Receptor, IGF Type 1 - biosynthesis ; Receptor, IGF Type 1 - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA Interference ; RNA, Messenger - genetics ; RNA, Small Interfering - pharmacology ; Transfection</subject><ispartof>Acta oncologica, 2010, Vol.49 (1), p.70-75</ispartof><rights>Informa UK Ltd 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-8b594a5c2f41d54165ad95cae085913259f73f943213773e01d9cc27eba9d74e3</citedby><cites>FETCH-LOGICAL-c395t-8b594a5c2f41d54165ad95cae085913259f73f943213773e01d9cc27eba9d74e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/02841860903334429$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/02841860903334429$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,61194,61375</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20001499$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yavari, Kamal</creatorcontrib><creatorcontrib>Taghikhani, Mohammad</creatorcontrib><creatorcontrib>Maragheh, Mohammad Ghannadi</creatorcontrib><creatorcontrib>Mesbah-Namin, Seyed A.</creatorcontrib><creatorcontrib>Babaei, Mohammad Hosein</creatorcontrib><creatorcontrib>Arfaee, Ali Jabbary</creatorcontrib><creatorcontrib>Madani, Hossein</creatorcontrib><creatorcontrib>Mirzaei, Hamid Reza</creatorcontrib><title>SiRNA-mediated IGF-1R inhibition sensitizes human colon cancer SW480 cells to radiation</title><title>Acta oncologica</title><addtitle>Acta Oncol</addtitle><description>Abstract
Purpose. Insulin like growth factor receptor 1 (IGF-1R) is well-documented to play a key role in radiation response and tumor radiosensitivity, thus offering an attractive clinic drug target to enhance tumor sensitivity to anti-cancer radiotherapy. Material and methods. Human colon carcinoma SW480 cells were transfected with the specific small interference RNA (siRNA) expression vector (pkD-shRNA-IGF-1R-V2) designed to target IGF-1R mRNA. The expression of IGF-1R mRNA and its protein among the transfected and untransfected cells were detected by semi-quantitative RT-PCR and ELISA assay. The changes in cell radiosensitivity were examined by MTT assay. Results. Transfection of mammalian expression vector pkD containing IGF-1R siRNA was shown to reduce IGF-1R mRNA levels by up to 95%. ELISA assay detected a similar inhibition of IGF-1R protein levels in cells transfected with IGF-1R siRNA. SW480 cells transfected with the expression vector for siRNA significantly rendered cells more sensitive to radiation and the highest radiation enhancement ratio was 2.02 ± 0.08. Conclusion. These data provide the first evidence that specific siRNA fragment (pkD-shRNA-IGF-1R-V2) targeting human IGF-1R mRNA is able to enhance colon cancer radiosensitivity. Also results indicated that, combining IGF-1R siRNA and radiation significantly enhances antitumor efficacy compared with either modality alone.</description><subject>Cell Line, Tumor</subject><subject>Colonic Neoplasms - genetics</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Humans</subject><subject>Radiation Tolerance - genetics</subject><subject>Radiation-Sensitizing Agents - pharmacology</subject><subject>Receptor, IGF Type 1 - antagonists & inhibitors</subject><subject>Receptor, IGF Type 1 - biosynthesis</subject><subject>Receptor, IGF Type 1 - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA Interference</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Small Interfering - pharmacology</subject><subject>Transfection</subject><issn>0284-186X</issn><issn>1651-226X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LAzEQhoMotlZ_gBfJzdNqPjcbPBWxtVAUWqW9Ldlslk3Zj5rsHvTXm6XVi-BphpnnfZl5AbjG6I5iJO8RSRhOYiQRpZQxIk_AGMccR4TE21MwHvZRALYjcOH9DiFEqODnYERCi5mUY7BZ29XLNKpNblVncriYzyK8grYpbWY72zbQm8aH7st4WPa1aqBuqzDWqtHGwfWGJQhqU1Uedi10avAJsktwVqjKm6tjnYD32dPb43O0fJ0vHqfLSFPJuyjJuGSKa1IwnHMWjle55FoZlHCJKeGyELSQjBJMhaAG4VxqTYTJlMwFM3QCbg--e9d-9MZ3aW39cI5qTNv7VFCacCFjEkh8ILVrvXemSPfO1sp9philQ5zpnziD5ubo3mchol_FT34BeDgAtilaV6vSqKortXIm3bW9a8Lr_9h_A0h2gHE</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Yavari, Kamal</creator><creator>Taghikhani, Mohammad</creator><creator>Maragheh, Mohammad Ghannadi</creator><creator>Mesbah-Namin, Seyed A.</creator><creator>Babaei, Mohammad Hosein</creator><creator>Arfaee, Ali Jabbary</creator><creator>Madani, Hossein</creator><creator>Mirzaei, Hamid Reza</creator><general>Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2010</creationdate><title>SiRNA-mediated IGF-1R inhibition sensitizes human colon cancer SW480 cells to radiation</title><author>Yavari, Kamal ; Taghikhani, Mohammad ; Maragheh, Mohammad Ghannadi ; Mesbah-Namin, Seyed A. ; Babaei, Mohammad Hosein ; Arfaee, Ali Jabbary ; Madani, Hossein ; Mirzaei, Hamid Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-8b594a5c2f41d54165ad95cae085913259f73f943213773e01d9cc27eba9d74e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Cell Line, Tumor</topic><topic>Colonic Neoplasms - genetics</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Humans</topic><topic>Radiation Tolerance - genetics</topic><topic>Radiation-Sensitizing Agents - pharmacology</topic><topic>Receptor, IGF Type 1 - antagonists & inhibitors</topic><topic>Receptor, IGF Type 1 - biosynthesis</topic><topic>Receptor, IGF Type 1 - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA Interference</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Small Interfering - pharmacology</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yavari, Kamal</creatorcontrib><creatorcontrib>Taghikhani, Mohammad</creatorcontrib><creatorcontrib>Maragheh, Mohammad Ghannadi</creatorcontrib><creatorcontrib>Mesbah-Namin, Seyed A.</creatorcontrib><creatorcontrib>Babaei, Mohammad Hosein</creatorcontrib><creatorcontrib>Arfaee, Ali Jabbary</creatorcontrib><creatorcontrib>Madani, Hossein</creatorcontrib><creatorcontrib>Mirzaei, Hamid Reza</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta oncologica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yavari, Kamal</au><au>Taghikhani, Mohammad</au><au>Maragheh, Mohammad Ghannadi</au><au>Mesbah-Namin, Seyed A.</au><au>Babaei, Mohammad Hosein</au><au>Arfaee, Ali Jabbary</au><au>Madani, Hossein</au><au>Mirzaei, Hamid Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SiRNA-mediated IGF-1R inhibition sensitizes human colon cancer SW480 cells to radiation</atitle><jtitle>Acta oncologica</jtitle><addtitle>Acta Oncol</addtitle><date>2010</date><risdate>2010</risdate><volume>49</volume><issue>1</issue><spage>70</spage><epage>75</epage><pages>70-75</pages><issn>0284-186X</issn><eissn>1651-226X</eissn><abstract>Abstract
Purpose. Insulin like growth factor receptor 1 (IGF-1R) is well-documented to play a key role in radiation response and tumor radiosensitivity, thus offering an attractive clinic drug target to enhance tumor sensitivity to anti-cancer radiotherapy. Material and methods. Human colon carcinoma SW480 cells were transfected with the specific small interference RNA (siRNA) expression vector (pkD-shRNA-IGF-1R-V2) designed to target IGF-1R mRNA. The expression of IGF-1R mRNA and its protein among the transfected and untransfected cells were detected by semi-quantitative RT-PCR and ELISA assay. The changes in cell radiosensitivity were examined by MTT assay. Results. Transfection of mammalian expression vector pkD containing IGF-1R siRNA was shown to reduce IGF-1R mRNA levels by up to 95%. ELISA assay detected a similar inhibition of IGF-1R protein levels in cells transfected with IGF-1R siRNA. SW480 cells transfected with the expression vector for siRNA significantly rendered cells more sensitive to radiation and the highest radiation enhancement ratio was 2.02 ± 0.08. Conclusion. These data provide the first evidence that specific siRNA fragment (pkD-shRNA-IGF-1R-V2) targeting human IGF-1R mRNA is able to enhance colon cancer radiosensitivity. Also results indicated that, combining IGF-1R siRNA and radiation significantly enhances antitumor efficacy compared with either modality alone.</abstract><cop>England</cop><pub>Informa UK Ltd. (Informa Healthcare, Taylor & Francis AS)</pub><pmid>20001499</pmid><doi>10.3109/02841860903334429</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Cell Line, Tumor Colonic Neoplasms - genetics Enzyme-Linked Immunosorbent Assay Humans Radiation Tolerance - genetics Radiation-Sensitizing Agents - pharmacology Receptor, IGF Type 1 - antagonists & inhibitors Receptor, IGF Type 1 - biosynthesis Receptor, IGF Type 1 - genetics Reverse Transcriptase Polymerase Chain Reaction RNA Interference RNA, Messenger - genetics RNA, Small Interfering - pharmacology Transfection |
| title | SiRNA-mediated IGF-1R inhibition sensitizes human colon cancer SW480 cells to radiation |
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