Trans-diamminedichlorplatinum (II)-modified probes for detection of picogram quantities of DNA
A novel simple nonradioactive method for detectionof specific nucleotide sequences has been developed. This method consists of the hybridization of a target DNA with a DNA probe modified with trans-diammine-dichlorplatinum(II) ( trans-DDP) followed by detection of DNA/DNA hybrids with affinity-isola...
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| Veröffentlicht in: | Analytical biochemistry 1992-10, Vol.206 (1), p.43-49 |
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| container_title | Analytical biochemistry |
| container_volume | 206 |
| creator | Kiseleva, Valentina I. Kolesnik, Tatjana B. Turchinsky, Michail F. Wagner, Laura L. Kovalenko, Victor A. Plaksin, Dmitrij Ju Koukalová, Blazena Kuhrová, Viera Brábec, Victor Poverenny, Alexander M. |
| description | A novel simple nonradioactive method for detectionof specific nucleotide sequences has been developed. This method consists of the hybridization of a target DNA with a DNA probe modified with
trans-diammine-dichlorplatinum(II) (
trans-DDP) followed by detection of DNA/DNA hybrids with affinity-isolated anti-DNA-
trans-DDP antibodies and poly-horseradish peroxidase-protein A conjugate. Major advantages of this approach are the low cost and the extreme simplicity of the labeling procedure, which involves only mixing of the reagents. The sensitivity of the proposed technique is sufficient to detect 0.8 pg of DNA in Southern blot hybridization and 25 fg in dot hybridization and permits colony screening. |
| doi_str_mv | 10.1016/S0003-2697(05)80008-9 |
| format | Article |
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trans-diammine-dichlorplatinum(II) (
trans-DDP) followed by detection of DNA/DNA hybrids with affinity-isolated anti-DNA-
trans-DDP antibodies and poly-horseradish peroxidase-protein A conjugate. Major advantages of this approach are the low cost and the extreme simplicity of the labeling procedure, which involves only mixing of the reagents. The sensitivity of the proposed technique is sufficient to detect 0.8 pg of DNA in Southern blot hybridization and 25 fg in dot hybridization and permits colony screening.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1016/S0003-2697(05)80008-9</identifier><identifier>PMID: 1456440</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Antibodies ; Bacteriophage lambda ; Blotting, Southern ; Cisplatin ; DNA - analysis ; DNA Probes ; DNA, Bacterial - analysis ; DNA, Bacterial - genetics ; DNA, Recombinant - analysis ; DNA, Recombinant - genetics ; DNA, Viral - analysis ; DNA, Viral - genetics ; Drug Stability ; Enzyme-Linked Immunosorbent Assay ; Escherichia coli - genetics ; Haptens ; Immunoenzyme Techniques ; Microchemistry - methods ; Nucleic Acid Hybridization - methods ; Phosphorus Radioisotopes ; Sensitivity and Specificity</subject><ispartof>Analytical biochemistry, 1992-10, Vol.206 (1), p.43-49</ispartof><rights>1992 Academic Press, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c457t-2f7bed4892300f372347d4d55a5047ac70229b88415b0ca9d88adc3024fd903e3</citedby><cites>FETCH-LOGICAL-c457t-2f7bed4892300f372347d4d55a5047ac70229b88415b0ca9d88adc3024fd903e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0003269705800089$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1456440$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kiseleva, Valentina I.</creatorcontrib><creatorcontrib>Kolesnik, Tatjana B.</creatorcontrib><creatorcontrib>Turchinsky, Michail F.</creatorcontrib><creatorcontrib>Wagner, Laura L.</creatorcontrib><creatorcontrib>Kovalenko, Victor A.</creatorcontrib><creatorcontrib>Plaksin, Dmitrij Ju</creatorcontrib><creatorcontrib>Koukalová, Blazena</creatorcontrib><creatorcontrib>Kuhrová, Viera</creatorcontrib><creatorcontrib>Brábec, Victor</creatorcontrib><creatorcontrib>Poverenny, Alexander M.</creatorcontrib><title>Trans-diamminedichlorplatinum (II)-modified probes for detection of picogram quantities of DNA</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>A novel simple nonradioactive method for detectionof specific nucleotide sequences has been developed. This method consists of the hybridization of a target DNA with a DNA probe modified with
trans-diammine-dichlorplatinum(II) (
trans-DDP) followed by detection of DNA/DNA hybrids with affinity-isolated anti-DNA-
trans-DDP antibodies and poly-horseradish peroxidase-protein A conjugate. Major advantages of this approach are the low cost and the extreme simplicity of the labeling procedure, which involves only mixing of the reagents. The sensitivity of the proposed technique is sufficient to detect 0.8 pg of DNA in Southern blot hybridization and 25 fg in dot hybridization and permits colony screening.</description><subject>Antibodies</subject><subject>Bacteriophage lambda</subject><subject>Blotting, Southern</subject><subject>Cisplatin</subject><subject>DNA - analysis</subject><subject>DNA Probes</subject><subject>DNA, Bacterial - analysis</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Recombinant - analysis</subject><subject>DNA, Recombinant - genetics</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Drug Stability</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Escherichia coli - genetics</subject><subject>Haptens</subject><subject>Immunoenzyme Techniques</subject><subject>Microchemistry - methods</subject><subject>Nucleic Acid Hybridization - methods</subject><subject>Phosphorus Radioisotopes</subject><subject>Sensitivity and Specificity</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1PxCAQhonRrOvqT9ikJ6OH6lCglJMx69cmRg_qVUKBKqYtK7Qm_ntZ1-jR02SYd5iZ50VojuEEAy5PHwCA5EUp-BGw4yplVS620BSDKHMgILbR9Feyi_ZifAPAmLJygibrQClM0fNjUH3MjVNd53prnH5tfVi1anD92GVHy-Vx3nnjGmdNtgq-tjFrfMiMHawenO8z32Qrp_1LUF32Pqp-cINLovR8cXe-j3Ya1UZ78BNn6Onq8nFxk9_eXy8X57e5powPedHw2hpaiYIANIQXhHJDDWOKAeVKcygKUVcVxawGrYSpKmU0gYI2RgCxZIYON_-mFd9HGwfZuaht26re-jFKTkiVLib_CnHJaSEwJCHbCHXwMQbbyFVwnQqfEoNcGyC_DZBruhKY_DZAitQ3_xkw1p01f10b4ql-tqnbhOPD2SCjdrbXCX1IRKXx7p8JX-mBlJE</recordid><startdate>19921001</startdate><enddate>19921001</enddate><creator>Kiseleva, Valentina I.</creator><creator>Kolesnik, Tatjana B.</creator><creator>Turchinsky, Michail F.</creator><creator>Wagner, Laura L.</creator><creator>Kovalenko, Victor A.</creator><creator>Plaksin, Dmitrij Ju</creator><creator>Koukalová, Blazena</creator><creator>Kuhrová, Viera</creator><creator>Brábec, Victor</creator><creator>Poverenny, Alexander M.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19921001</creationdate><title>Trans-diamminedichlorplatinum (II)-modified probes for detection of picogram quantities of DNA</title><author>Kiseleva, Valentina I. ; Kolesnik, Tatjana B. ; Turchinsky, Michail F. ; Wagner, Laura L. ; Kovalenko, Victor A. ; Plaksin, Dmitrij Ju ; Koukalová, Blazena ; Kuhrová, Viera ; Brábec, Victor ; Poverenny, Alexander M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c457t-2f7bed4892300f372347d4d55a5047ac70229b88415b0ca9d88adc3024fd903e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies</topic><topic>Bacteriophage lambda</topic><topic>Blotting, Southern</topic><topic>Cisplatin</topic><topic>DNA - analysis</topic><topic>DNA Probes</topic><topic>DNA, Bacterial - analysis</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Recombinant - analysis</topic><topic>DNA, Recombinant - genetics</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - genetics</topic><topic>Drug Stability</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Escherichia coli - genetics</topic><topic>Haptens</topic><topic>Immunoenzyme Techniques</topic><topic>Microchemistry - methods</topic><topic>Nucleic Acid Hybridization - methods</topic><topic>Phosphorus Radioisotopes</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kiseleva, Valentina I.</creatorcontrib><creatorcontrib>Kolesnik, Tatjana B.</creatorcontrib><creatorcontrib>Turchinsky, Michail F.</creatorcontrib><creatorcontrib>Wagner, Laura L.</creatorcontrib><creatorcontrib>Kovalenko, Victor A.</creatorcontrib><creatorcontrib>Plaksin, Dmitrij Ju</creatorcontrib><creatorcontrib>Koukalová, Blazena</creatorcontrib><creatorcontrib>Kuhrová, Viera</creatorcontrib><creatorcontrib>Brábec, Victor</creatorcontrib><creatorcontrib>Poverenny, Alexander M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kiseleva, Valentina I.</au><au>Kolesnik, Tatjana B.</au><au>Turchinsky, Michail F.</au><au>Wagner, Laura L.</au><au>Kovalenko, Victor A.</au><au>Plaksin, Dmitrij Ju</au><au>Koukalová, Blazena</au><au>Kuhrová, Viera</au><au>Brábec, Victor</au><au>Poverenny, Alexander M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trans-diamminedichlorplatinum (II)-modified probes for detection of picogram quantities of DNA</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1992-10-01</date><risdate>1992</risdate><volume>206</volume><issue>1</issue><spage>43</spage><epage>49</epage><pages>43-49</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><abstract>A novel simple nonradioactive method for detectionof specific nucleotide sequences has been developed. This method consists of the hybridization of a target DNA with a DNA probe modified with
trans-diammine-dichlorplatinum(II) (
trans-DDP) followed by detection of DNA/DNA hybrids with affinity-isolated anti-DNA-
trans-DDP antibodies and poly-horseradish peroxidase-protein A conjugate. Major advantages of this approach are the low cost and the extreme simplicity of the labeling procedure, which involves only mixing of the reagents. The sensitivity of the proposed technique is sufficient to detect 0.8 pg of DNA in Southern blot hybridization and 25 fg in dot hybridization and permits colony screening.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>1456440</pmid><doi>10.1016/S0003-2697(05)80008-9</doi><tpages>7</tpages></addata></record> |
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| ispartof | Analytical biochemistry, 1992-10, Vol.206 (1), p.43-49 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Antibodies Bacteriophage lambda Blotting, Southern Cisplatin DNA - analysis DNA Probes DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Recombinant - analysis DNA, Recombinant - genetics DNA, Viral - analysis DNA, Viral - genetics Drug Stability Enzyme-Linked Immunosorbent Assay Escherichia coli - genetics Haptens Immunoenzyme Techniques Microchemistry - methods Nucleic Acid Hybridization - methods Phosphorus Radioisotopes Sensitivity and Specificity |
| title | Trans-diamminedichlorplatinum (II)-modified probes for detection of picogram quantities of DNA |
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