NALP expression in Paneth cells provides a novel track in IBD signaling
Purpose Paneth cells are part of the innate mucosal immunity of the gut with possible regulatory function. This study intends to identify the gene expression pattern of the orthotopic and metaplastic Paneth cells, searching for differences between metaplastic occurrence between Crohn's disease...
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| Veröffentlicht in: | Langenbeck's archives of surgery 2010-04, Vol.395 (4), p.351-357 |
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| container_issue | 4 |
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| container_title | Langenbeck's archives of surgery |
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| creator | Slavova, Nadia Drescher, Anja Visekruna, Alexander Dullat, Sonja Kroesen, Anton J. Ritz, Joerg-Peter Buhr, Heinz J. |
| description | Purpose
Paneth cells are part of the innate mucosal immunity of the gut with possible regulatory function. This study intends to identify the gene expression pattern of the orthotopic and metaplastic Paneth cells, searching for differences between metaplastic occurrence between Crohn's disease and ulcerative colitis.
Methods
Paneth cells were collected in RNAse-free conditions via micro dissection. RNA isolation and super amplification was followed by microarray analysis of whole genome expression activity of the orthotopic and metaplastic Paneth cells. Immunohistology of β-catenin and Frizzled-5 receptor was performed.
Results
Histological analysis showed no morphological or secretory change (Frizzled-5 receptor and β-catenin) in orthotopic and metaplastic Paneth cells. Microarray analysis indicated an increased, but not mutant activation of Wnt/β-catenin signaling and firstly showed expression of NALP 1, 7, 8 and 11 in metaplastic Paneth cells.
Conclusions
Paneth cells might play a NALP-mediated role in the pathogenesis of IBD. |
| doi_str_mv | 10.1007/s00423-010-0611-8 |
| format | Article |
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Paneth cells are part of the innate mucosal immunity of the gut with possible regulatory function. This study intends to identify the gene expression pattern of the orthotopic and metaplastic Paneth cells, searching for differences between metaplastic occurrence between Crohn's disease and ulcerative colitis.
Methods
Paneth cells were collected in RNAse-free conditions via micro dissection. RNA isolation and super amplification was followed by microarray analysis of whole genome expression activity of the orthotopic and metaplastic Paneth cells. Immunohistology of β-catenin and Frizzled-5 receptor was performed.
Results
Histological analysis showed no morphological or secretory change (Frizzled-5 receptor and β-catenin) in orthotopic and metaplastic Paneth cells. Microarray analysis indicated an increased, but not mutant activation of Wnt/β-catenin signaling and firstly showed expression of NALP 1, 7, 8 and 11 in metaplastic Paneth cells.
Conclusions
Paneth cells might play a NALP-mediated role in the pathogenesis of IBD.</description><identifier>ISSN: 1435-2443</identifier><identifier>EISSN: 1435-2451</identifier><identifier>DOI: 10.1007/s00423-010-0611-8</identifier><identifier>PMID: 20333398</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Abdominal Surgery ; Adaptor Proteins, Signal Transducing - biosynthesis ; Cardiac Surgery ; Colitis, Ulcerative - metabolism ; Crohn Disease - metabolism ; Gene Expression Profiling ; General Surgery ; Humans ; Ileum - metabolism ; Immunohistochemistry ; Inflammatory Bowel Diseases - metabolism ; Medicine ; Medicine & Public Health ; Original Article ; Paneth Cells - metabolism ; Paneth Cells - pathology ; Protein Biosynthesis ; Proteins - metabolism ; Thoracic Surgery ; Traumatic Surgery ; Vascular Surgery</subject><ispartof>Langenbeck's archives of surgery, 2010-04, Vol.395 (4), p.351-357</ispartof><rights>Springer-Verlag 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-8e0283adb1d05e23d6c4495714cebe25f4c78689a09705bf1b6d673b2946695a3</citedby><cites>FETCH-LOGICAL-c343t-8e0283adb1d05e23d6c4495714cebe25f4c78689a09705bf1b6d673b2946695a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00423-010-0611-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00423-010-0611-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20333398$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Slavova, Nadia</creatorcontrib><creatorcontrib>Drescher, Anja</creatorcontrib><creatorcontrib>Visekruna, Alexander</creatorcontrib><creatorcontrib>Dullat, Sonja</creatorcontrib><creatorcontrib>Kroesen, Anton J.</creatorcontrib><creatorcontrib>Ritz, Joerg-Peter</creatorcontrib><creatorcontrib>Buhr, Heinz J.</creatorcontrib><title>NALP expression in Paneth cells provides a novel track in IBD signaling</title><title>Langenbeck's archives of surgery</title><addtitle>Langenbecks Arch Surg</addtitle><addtitle>Langenbecks Arch Surg</addtitle><description>Purpose
Paneth cells are part of the innate mucosal immunity of the gut with possible regulatory function. This study intends to identify the gene expression pattern of the orthotopic and metaplastic Paneth cells, searching for differences between metaplastic occurrence between Crohn's disease and ulcerative colitis.
Methods
Paneth cells were collected in RNAse-free conditions via micro dissection. RNA isolation and super amplification was followed by microarray analysis of whole genome expression activity of the orthotopic and metaplastic Paneth cells. Immunohistology of β-catenin and Frizzled-5 receptor was performed.
Results
Histological analysis showed no morphological or secretory change (Frizzled-5 receptor and β-catenin) in orthotopic and metaplastic Paneth cells. Microarray analysis indicated an increased, but not mutant activation of Wnt/β-catenin signaling and firstly showed expression of NALP 1, 7, 8 and 11 in metaplastic Paneth cells.
Conclusions
Paneth cells might play a NALP-mediated role in the pathogenesis of IBD.</description><subject>Abdominal Surgery</subject><subject>Adaptor Proteins, Signal Transducing - biosynthesis</subject><subject>Cardiac Surgery</subject><subject>Colitis, Ulcerative - metabolism</subject><subject>Crohn Disease - metabolism</subject><subject>Gene Expression Profiling</subject><subject>General Surgery</subject><subject>Humans</subject><subject>Ileum - metabolism</subject><subject>Immunohistochemistry</subject><subject>Inflammatory Bowel Diseases - metabolism</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Original Article</subject><subject>Paneth Cells - metabolism</subject><subject>Paneth Cells - pathology</subject><subject>Protein Biosynthesis</subject><subject>Proteins - metabolism</subject><subject>Thoracic Surgery</subject><subject>Traumatic Surgery</subject><subject>Vascular Surgery</subject><issn>1435-2443</issn><issn>1435-2451</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kL1OwzAURi0EoqXwACzIG1Pg-ieOPZYCpVIFHWC2nMQpKWlS7KSCt8dRoCN38ZV87qdPB6FLAjcEILn1AJyyCAhEIAiJ5BEaE87iiPKYHB92zkbozPsNAIhE8VM0osDCKDlG8-fpcoXt185Z78umxmWNV6a27TvObFV5vHPNvsytxwbXzd5WuHUm--ixxd099uW6NlVZr8_RSWEqby9-3wl6e3x4nT1Fy5f5YjZdRhnjrI2kBSqZyVOSQ2wpy0XGuYoTwjObWhoXPEukkMqASiBOC5KKXCQspYoLoWLDJuh6yA29PjvrW70tfd80dG46rxPGJJegRCDJQGau8d7ZQu9cuTXuWxPQvT496NNBn-71aRlurn7Tu3Rr88PFn68A0AHw4ateW6c3TeeCAv9P6g8kNnh9</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Slavova, Nadia</creator><creator>Drescher, Anja</creator><creator>Visekruna, Alexander</creator><creator>Dullat, Sonja</creator><creator>Kroesen, Anton J.</creator><creator>Ritz, Joerg-Peter</creator><creator>Buhr, Heinz J.</creator><general>Springer-Verlag</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100401</creationdate><title>NALP expression in Paneth cells provides a novel track in IBD signaling</title><author>Slavova, Nadia ; Drescher, Anja ; Visekruna, Alexander ; Dullat, Sonja ; Kroesen, Anton J. ; Ritz, Joerg-Peter ; Buhr, Heinz J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-8e0283adb1d05e23d6c4495714cebe25f4c78689a09705bf1b6d673b2946695a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Abdominal Surgery</topic><topic>Adaptor Proteins, Signal Transducing - biosynthesis</topic><topic>Cardiac Surgery</topic><topic>Colitis, Ulcerative - metabolism</topic><topic>Crohn Disease - metabolism</topic><topic>Gene Expression Profiling</topic><topic>General Surgery</topic><topic>Humans</topic><topic>Ileum - metabolism</topic><topic>Immunohistochemistry</topic><topic>Inflammatory Bowel Diseases - metabolism</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Original Article</topic><topic>Paneth Cells - metabolism</topic><topic>Paneth Cells - pathology</topic><topic>Protein Biosynthesis</topic><topic>Proteins - metabolism</topic><topic>Thoracic Surgery</topic><topic>Traumatic Surgery</topic><topic>Vascular Surgery</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Slavova, Nadia</creatorcontrib><creatorcontrib>Drescher, Anja</creatorcontrib><creatorcontrib>Visekruna, Alexander</creatorcontrib><creatorcontrib>Dullat, Sonja</creatorcontrib><creatorcontrib>Kroesen, Anton J.</creatorcontrib><creatorcontrib>Ritz, Joerg-Peter</creatorcontrib><creatorcontrib>Buhr, Heinz J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Langenbeck's archives of surgery</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Slavova, Nadia</au><au>Drescher, Anja</au><au>Visekruna, Alexander</au><au>Dullat, Sonja</au><au>Kroesen, Anton J.</au><au>Ritz, Joerg-Peter</au><au>Buhr, Heinz J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>NALP expression in Paneth cells provides a novel track in IBD signaling</atitle><jtitle>Langenbeck's archives of surgery</jtitle><stitle>Langenbecks Arch Surg</stitle><addtitle>Langenbecks Arch Surg</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>395</volume><issue>4</issue><spage>351</spage><epage>357</epage><pages>351-357</pages><issn>1435-2443</issn><eissn>1435-2451</eissn><abstract>Purpose
Paneth cells are part of the innate mucosal immunity of the gut with possible regulatory function. This study intends to identify the gene expression pattern of the orthotopic and metaplastic Paneth cells, searching for differences between metaplastic occurrence between Crohn's disease and ulcerative colitis.
Methods
Paneth cells were collected in RNAse-free conditions via micro dissection. RNA isolation and super amplification was followed by microarray analysis of whole genome expression activity of the orthotopic and metaplastic Paneth cells. Immunohistology of β-catenin and Frizzled-5 receptor was performed.
Results
Histological analysis showed no morphological or secretory change (Frizzled-5 receptor and β-catenin) in orthotopic and metaplastic Paneth cells. Microarray analysis indicated an increased, but not mutant activation of Wnt/β-catenin signaling and firstly showed expression of NALP 1, 7, 8 and 11 in metaplastic Paneth cells.
Conclusions
Paneth cells might play a NALP-mediated role in the pathogenesis of IBD.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><pmid>20333398</pmid><doi>10.1007/s00423-010-0611-8</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Abdominal Surgery Adaptor Proteins, Signal Transducing - biosynthesis Cardiac Surgery Colitis, Ulcerative - metabolism Crohn Disease - metabolism Gene Expression Profiling General Surgery Humans Ileum - metabolism Immunohistochemistry Inflammatory Bowel Diseases - metabolism Medicine Medicine & Public Health Original Article Paneth Cells - metabolism Paneth Cells - pathology Protein Biosynthesis Proteins - metabolism Thoracic Surgery Traumatic Surgery Vascular Surgery |
| title | NALP expression in Paneth cells provides a novel track in IBD signaling |
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