Amplified fragment length polymorphism (AFLP) analysis is useful for distinguishing Leishmania species of visceral and cutaneous forms
The Leishmania strains belonging to cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) have been reported to possess close homology in genome profiles. To confirm this on genetic basis an attempt was made to differentiate Leishmania major; Leishmania tropica and Leishmania donovani genetic...
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| Veröffentlicht in: | Acta tropica 2010-02, Vol.113 (2), p.202-206 |
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| creator | Kumar, Awanish Boggula, Vijay Raju Misra, Pragya Sundar, Shyam Shasany, Ajit Kumar Dube, Anuradha |
| description | The
Leishmania strains belonging to cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) have been reported to possess close homology in genome profiles. To confirm this on genetic basis an attempt was made to differentiate
Leishmania major;
Leishmania tropica and
Leishmania donovani genetically for the first time using amplified fragment length polymorphism (AFLP)—a high throughput DNA fingerprinting technique. The objective of this research work was to identify DNA markers of CL and VL. Ten combinations of selective primers detect a total of 1487 informative AFLP marker. Percentage of polymorphism was 45.12%. Three hundred and thirty-seven unique AFLP markers were also identified in three species of
Leishmania. A clear distinction was revealed between
L. major and
L. donovani. It was inferred by AFLP analysis that a higher rate of polymorphisms occurred among
Leishmania species which indicate the distinguished pattern of the disease cause by
Leishmania, i.e. VL and CL. Analysis based on polymorphic AFLP markers revealed considerably high genetic variation among the genome of these species which was sufficient to distinguish between CL and VL. |
| doi_str_mv | 10.1016/j.actatropica.2009.10.006 |
| format | Article |
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Leishmania strains belonging to cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) have been reported to possess close homology in genome profiles. To confirm this on genetic basis an attempt was made to differentiate
Leishmania major;
Leishmania tropica and
Leishmania donovani genetically for the first time using amplified fragment length polymorphism (AFLP)—a high throughput DNA fingerprinting technique. The objective of this research work was to identify DNA markers of CL and VL. Ten combinations of selective primers detect a total of 1487 informative AFLP marker. Percentage of polymorphism was 45.12%. Three hundred and thirty-seven unique AFLP markers were also identified in three species of
Leishmania. A clear distinction was revealed between
L. major and
L. donovani. It was inferred by AFLP analysis that a higher rate of polymorphisms occurred among
Leishmania species which indicate the distinguished pattern of the disease cause by
Leishmania, i.e. VL and CL. Analysis based on polymorphic AFLP markers revealed considerably high genetic variation among the genome of these species which was sufficient to distinguish between CL and VL.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2009.10.006</identifier><identifier>PMID: 19854144</identifier><identifier>CODEN: ACTRAQ</identifier><language>eng</language><publisher>Oxford: Elsevier B.V</publisher><subject>AFLP ; Amplified Fragment Length Polymorphism Analysis - methods ; Animals ; Biological and medical sciences ; Cutaneous leishmaniasis ; DNA Fingerprinting - methods ; Fingerprinting ; General aspects ; Genetic Markers - genetics ; Genetic polymorphism ; Genome, Protozoan ; Human protozoal diseases ; Humans ; Infectious diseases ; Leishmania - classification ; Leishmania - genetics ; Leishmania - isolation & purification ; Leishmania donovani ; Leishmania donovani - classification ; Leishmania donovani - genetics ; Leishmania donovani - isolation & purification ; Leishmania major ; Leishmania major - classification ; Leishmania major - genetics ; Leishmania major - isolation & purification ; Leishmania tropica ; Leishmania tropica - classification ; Leishmania tropica - genetics ; Leishmania tropica - isolation & purification ; Leishmaniasis, Cutaneous - diagnosis ; Leishmaniasis, Cutaneous - parasitology ; Leishmaniasis, Visceral - diagnosis ; Leishmaniasis, Visceral - parasitology ; Leshmaniasis ; Medical sciences ; Parasitic diseases ; Polymorphism, Genetic ; Protozoal diseases ; Species Specificity ; Visceral leishmaniasis</subject><ispartof>Acta tropica, 2010-02, Vol.113 (2), p.202-206</ispartof><rights>2009 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright 2009 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c437t-961a7a5e6a0fea15b0b8f29682de86b505daabb2e07b04bdf4dfe86e32cbdb663</citedby><cites>FETCH-LOGICAL-c437t-961a7a5e6a0fea15b0b8f29682de86b505daabb2e07b04bdf4dfe86e32cbdb663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X0900326X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22393690$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19854144$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kumar, Awanish</creatorcontrib><creatorcontrib>Boggula, Vijay Raju</creatorcontrib><creatorcontrib>Misra, Pragya</creatorcontrib><creatorcontrib>Sundar, Shyam</creatorcontrib><creatorcontrib>Shasany, Ajit Kumar</creatorcontrib><creatorcontrib>Dube, Anuradha</creatorcontrib><title>Amplified fragment length polymorphism (AFLP) analysis is useful for distinguishing Leishmania species of visceral and cutaneous forms</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>The
Leishmania strains belonging to cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) have been reported to possess close homology in genome profiles. To confirm this on genetic basis an attempt was made to differentiate
Leishmania major;
Leishmania tropica and
Leishmania donovani genetically for the first time using amplified fragment length polymorphism (AFLP)—a high throughput DNA fingerprinting technique. The objective of this research work was to identify DNA markers of CL and VL. Ten combinations of selective primers detect a total of 1487 informative AFLP marker. Percentage of polymorphism was 45.12%. Three hundred and thirty-seven unique AFLP markers were also identified in three species of
Leishmania. A clear distinction was revealed between
L. major and
L. donovani. It was inferred by AFLP analysis that a higher rate of polymorphisms occurred among
Leishmania species which indicate the distinguished pattern of the disease cause by
Leishmania, i.e. VL and CL. Analysis based on polymorphic AFLP markers revealed considerably high genetic variation among the genome of these species which was sufficient to distinguish between CL and VL.</description><subject>AFLP</subject><subject>Amplified Fragment Length Polymorphism Analysis - methods</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cutaneous leishmaniasis</subject><subject>DNA Fingerprinting - methods</subject><subject>Fingerprinting</subject><subject>General aspects</subject><subject>Genetic Markers - genetics</subject><subject>Genetic polymorphism</subject><subject>Genome, Protozoan</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Leishmania - classification</subject><subject>Leishmania - genetics</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - classification</subject><subject>Leishmania donovani - genetics</subject><subject>Leishmania donovani - isolation & purification</subject><subject>Leishmania major</subject><subject>Leishmania major - classification</subject><subject>Leishmania major - genetics</subject><subject>Leishmania major - isolation & purification</subject><subject>Leishmania tropica</subject><subject>Leishmania tropica - classification</subject><subject>Leishmania tropica - genetics</subject><subject>Leishmania tropica - isolation & purification</subject><subject>Leishmaniasis, Cutaneous - diagnosis</subject><subject>Leishmaniasis, Cutaneous - parasitology</subject><subject>Leishmaniasis, Visceral - diagnosis</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Leshmaniasis</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Polymorphism, Genetic</subject><subject>Protozoal diseases</subject><subject>Species Specificity</subject><subject>Visceral leishmaniasis</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU2P0zAQhiMEYrsLfwGZA7twSLGdxEmOVcUCUiU4gMTN8se4deV84ElW6h_gd-PQCjghJEvj8TzveDRvlr1kdM0oE2-Pa2UmNcVh9EatOaVtel9TKh5lK9bURS54VT7OVpRSltdUfLvKrhGPKeN1xZ9mV6xtqpKV5Sr7senG4J0HS1xU-w76iQTo99OBjEM4dUMcDx478npzv_v8hqhehRN6JOnMCG4OxA2RWI-T7_ezx0MKZAfp0qneK4IjGA9IBkcePBqIKqQmlph5Uj0MMy76Dp9lT5wKCM8v8Sb7ev_uy_ZDvvv0_uN2s8tNWdRT3gqmalWBUNSBYpWmunG8FQ230Ahd0coqpTUHWmtaautK61IBCm601UIUN9ndue8Yh-8z4CS7ZaoQzsPIuiiakrNf5O0_Sc4KTnldJ7A9gyYOiBGcHKPvVDxJRuVilzzKv-ySi11LKdmVtC8un8y6A_tHefEnAa8ugEKjQrKoNx5_c5wXbSFamrjtmYO0vAcPUWJae2_A-ghmknbw_zHOT0uVvR4</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Kumar, Awanish</creator><creator>Boggula, Vijay Raju</creator><creator>Misra, Pragya</creator><creator>Sundar, Shyam</creator><creator>Shasany, Ajit Kumar</creator><creator>Dube, Anuradha</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>H97</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>Amplified fragment length polymorphism (AFLP) analysis is useful for distinguishing Leishmania species of visceral and cutaneous forms</title><author>Kumar, Awanish ; Boggula, Vijay Raju ; Misra, Pragya ; Sundar, Shyam ; Shasany, Ajit Kumar ; Dube, Anuradha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c437t-961a7a5e6a0fea15b0b8f29682de86b505daabb2e07b04bdf4dfe86e32cbdb663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>AFLP</topic><topic>Amplified Fragment Length Polymorphism Analysis - methods</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cutaneous leishmaniasis</topic><topic>DNA Fingerprinting - methods</topic><topic>Fingerprinting</topic><topic>General aspects</topic><topic>Genetic Markers - genetics</topic><topic>Genetic polymorphism</topic><topic>Genome, Protozoan</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Leishmania - classification</topic><topic>Leishmania - genetics</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - classification</topic><topic>Leishmania donovani - genetics</topic><topic>Leishmania donovani - isolation & purification</topic><topic>Leishmania major</topic><topic>Leishmania major - classification</topic><topic>Leishmania major - genetics</topic><topic>Leishmania major - isolation & purification</topic><topic>Leishmania tropica</topic><topic>Leishmania tropica - classification</topic><topic>Leishmania tropica - genetics</topic><topic>Leishmania tropica - isolation & purification</topic><topic>Leishmaniasis, Cutaneous - diagnosis</topic><topic>Leishmaniasis, Cutaneous - parasitology</topic><topic>Leishmaniasis, Visceral - diagnosis</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>Leshmaniasis</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Polymorphism, Genetic</topic><topic>Protozoal diseases</topic><topic>Species Specificity</topic><topic>Visceral leishmaniasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kumar, Awanish</creatorcontrib><creatorcontrib>Boggula, Vijay Raju</creatorcontrib><creatorcontrib>Misra, Pragya</creatorcontrib><creatorcontrib>Sundar, Shyam</creatorcontrib><creatorcontrib>Shasany, Ajit Kumar</creatorcontrib><creatorcontrib>Dube, Anuradha</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kumar, Awanish</au><au>Boggula, Vijay Raju</au><au>Misra, Pragya</au><au>Sundar, Shyam</au><au>Shasany, Ajit Kumar</au><au>Dube, Anuradha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Amplified fragment length polymorphism (AFLP) analysis is useful for distinguishing Leishmania species of visceral and cutaneous forms</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2010-02-01</date><risdate>2010</risdate><volume>113</volume><issue>2</issue><spage>202</spage><epage>206</epage><pages>202-206</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><coden>ACTRAQ</coden><abstract>The
Leishmania strains belonging to cutaneous leishmaniasis (CL) and visceral leishmaniasis (VL) have been reported to possess close homology in genome profiles. To confirm this on genetic basis an attempt was made to differentiate
Leishmania major;
Leishmania tropica and
Leishmania donovani genetically for the first time using amplified fragment length polymorphism (AFLP)—a high throughput DNA fingerprinting technique. The objective of this research work was to identify DNA markers of CL and VL. Ten combinations of selective primers detect a total of 1487 informative AFLP marker. Percentage of polymorphism was 45.12%. Three hundred and thirty-seven unique AFLP markers were also identified in three species of
Leishmania. A clear distinction was revealed between
L. major and
L. donovani. It was inferred by AFLP analysis that a higher rate of polymorphisms occurred among
Leishmania species which indicate the distinguished pattern of the disease cause by
Leishmania, i.e. VL and CL. Analysis based on polymorphic AFLP markers revealed considerably high genetic variation among the genome of these species which was sufficient to distinguish between CL and VL.</abstract><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>19854144</pmid><doi>10.1016/j.actatropica.2009.10.006</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0001-706X |
| ispartof | Acta tropica, 2010-02, Vol.113 (2), p.202-206 |
| issn | 0001-706X 1873-6254 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | AFLP Amplified Fragment Length Polymorphism Analysis - methods Animals Biological and medical sciences Cutaneous leishmaniasis DNA Fingerprinting - methods Fingerprinting General aspects Genetic Markers - genetics Genetic polymorphism Genome, Protozoan Human protozoal diseases Humans Infectious diseases Leishmania - classification Leishmania - genetics Leishmania - isolation & purification Leishmania donovani Leishmania donovani - classification Leishmania donovani - genetics Leishmania donovani - isolation & purification Leishmania major Leishmania major - classification Leishmania major - genetics Leishmania major - isolation & purification Leishmania tropica Leishmania tropica - classification Leishmania tropica - genetics Leishmania tropica - isolation & purification Leishmaniasis, Cutaneous - diagnosis Leishmaniasis, Cutaneous - parasitology Leishmaniasis, Visceral - diagnosis Leishmaniasis, Visceral - parasitology Leshmaniasis Medical sciences Parasitic diseases Polymorphism, Genetic Protozoal diseases Species Specificity Visceral leishmaniasis |
| title | Amplified fragment length polymorphism (AFLP) analysis is useful for distinguishing Leishmania species of visceral and cutaneous forms |
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