Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children
To explore the pathophysiology of acute lung injury in children. Prospective cohort study. Regional University Hospital, pediatric intensive care unit. Children without a preexisting lung injury who developed acute lung injury and were intubated were eligible for the study. Children without lung inj...
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| Veröffentlicht in: | Pediatric critical care medicine 2010-01, Vol.11 (1), p.82-91 |
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| creator | Todd, David A Marsh, Michael J George, Anne Henderson, Neil G Barr, Heather Sebastian, Seby Clark, Graeme T Koster, Grielof Clark, Howard W Postle, Anthony D |
| description | To explore the pathophysiology of acute lung injury in children.
Prospective cohort study.
Regional University Hospital, pediatric intensive care unit.
Children without a preexisting lung injury who developed acute lung injury and were intubated were eligible for the study. Children without lung injury and intubated for minor surgical procedures acted as controls.
Bronchoalveolar lavage fluid and blood were collected on days 1 to 4, weekly, and immediately before extubation during acute lung injury. Molecular species compositions of phosphatidylcholine were determined by electrospray ionization mass spectrometry of lipid extracts of bronchoalveolar lavage fluid supernatants. Surfactant proteins A, B, and D and interleukin-8 were measured in bronchoalveolar lavage fluid and plasma by enzyme-linked immunosorbent assay and Western blotting.
Eighteen children with acute lung injury were enrolled in the study and compared with eight controls. In children with acute lung injury, there were significant changes in the bronchoalveolar lavage fluid phosphatidylcholine species. Bronchoalveolar lavage fluid dipalmitoyl phosphatidylcholine (PC 16:0/16:0) and palmitoyl-myristoyl phosphatidylcholine (PC 16:0/14:0) significantly deceased during acute lung injury (p < .001 and p < .001, respectively), whereas oleoyl-linoleoyl PC (18:1/18:2), palmitoyl-linoleoyl PC (16:0/18:2) and stearoyl-linoleoyl PC (18:0/18:2) characteristic of plasma PC were significantly increased (p < .05, p < .02, and p < .05 respectively), as well as palmitoyl-oleoyl PC (16:0/18:1), and stearoyl-arachidonoyl PC (18:0/20:4) which are characteristic of cell membranes (p < .02, and p < .02, respectively). There were no significant changes to bronchoalveolar lavage fluid, surfactant protein A or B levels compared with controls during acute lung injury, whereas bronchoalveolar lavage fluid, surfactant protein D, and interleukin-8 levels significantly increased (p < .05 and p < .02, respectively). In plasma during acute lung injury, there were significant increases in surfactant proteins A, B, and D, and interleukin-8 (p < .001, p < .001, p < .05, and p < .001, respectively).
Changes to the phosphatidylcholine profile, surfactant proteins, and inflammatory markers of bronchoalveolar lavage fluid and plasma in children with acute lung injury are consistent with an alveolar/blood leakage and inflammatory cell membrane degradation products. These changes are due to alveolar capillary membrane damage and cel |
| doi_str_mv | 10.1097/PCC.0b013e3181ae5a4c |
| format | Article |
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Prospective cohort study.
Regional University Hospital, pediatric intensive care unit.
Children without a preexisting lung injury who developed acute lung injury and were intubated were eligible for the study. Children without lung injury and intubated for minor surgical procedures acted as controls.
Bronchoalveolar lavage fluid and blood were collected on days 1 to 4, weekly, and immediately before extubation during acute lung injury. Molecular species compositions of phosphatidylcholine were determined by electrospray ionization mass spectrometry of lipid extracts of bronchoalveolar lavage fluid supernatants. Surfactant proteins A, B, and D and interleukin-8 were measured in bronchoalveolar lavage fluid and plasma by enzyme-linked immunosorbent assay and Western blotting.
Eighteen children with acute lung injury were enrolled in the study and compared with eight controls. In children with acute lung injury, there were significant changes in the bronchoalveolar lavage fluid phosphatidylcholine species. Bronchoalveolar lavage fluid dipalmitoyl phosphatidylcholine (PC 16:0/16:0) and palmitoyl-myristoyl phosphatidylcholine (PC 16:0/14:0) significantly deceased during acute lung injury (p < .001 and p < .001, respectively), whereas oleoyl-linoleoyl PC (18:1/18:2), palmitoyl-linoleoyl PC (16:0/18:2) and stearoyl-linoleoyl PC (18:0/18:2) characteristic of plasma PC were significantly increased (p < .05, p < .02, and p < .05 respectively), as well as palmitoyl-oleoyl PC (16:0/18:1), and stearoyl-arachidonoyl PC (18:0/20:4) which are characteristic of cell membranes (p < .02, and p < .02, respectively). There were no significant changes to bronchoalveolar lavage fluid, surfactant protein A or B levels compared with controls during acute lung injury, whereas bronchoalveolar lavage fluid, surfactant protein D, and interleukin-8 levels significantly increased (p < .05 and p < .02, respectively). In plasma during acute lung injury, there were significant increases in surfactant proteins A, B, and D, and interleukin-8 (p < .001, p < .001, p < .05, and p < .001, respectively).
Changes to the phosphatidylcholine profile, surfactant proteins, and inflammatory markers of bronchoalveolar lavage fluid and plasma in children with acute lung injury are consistent with an alveolar/blood leakage and inflammatory cell membrane degradation products. These changes are due to alveolar capillary membrane damage and cellular infiltration.]]></description><identifier>ISSN: 1529-7535</identifier><identifier>DOI: 10.1097/PCC.0b013e3181ae5a4c</identifier><identifier>PMID: 19550365</identifier><language>eng</language><publisher>United States</publisher><subject>Acute Lung Injury - physiopathology ; Biomarkers - metabolism ; Bronchoalveolar Lavage Fluid - chemistry ; Child ; Child, Preschool ; Cohort Studies ; Female ; Humans ; Inflammation - diagnosis ; Inflammation - physiopathology ; Intensive Care Units, Pediatric ; Interleukin-8 - metabolism ; Intubation, Intratracheal ; Lipoproteins - metabolism ; Male ; Phospholipids - analysis ; Prospective Studies ; Pulmonary Surfactants - analysis ; Respiratory Distress Syndrome, Adult - physiopathology</subject><ispartof>Pediatric critical care medicine, 2010-01, Vol.11 (1), p.82-91</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c352t-765187b6b2d93fa42862c3ae548b3c7521cceb66b8c3ea7a65650da5a26d224c3</citedby><cites>FETCH-LOGICAL-c352t-765187b6b2d93fa42862c3ae548b3c7521cceb66b8c3ea7a65650da5a26d224c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19550365$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Todd, David A</creatorcontrib><creatorcontrib>Marsh, Michael J</creatorcontrib><creatorcontrib>George, Anne</creatorcontrib><creatorcontrib>Henderson, Neil G</creatorcontrib><creatorcontrib>Barr, Heather</creatorcontrib><creatorcontrib>Sebastian, Seby</creatorcontrib><creatorcontrib>Clark, Graeme T</creatorcontrib><creatorcontrib>Koster, Grielof</creatorcontrib><creatorcontrib>Clark, Howard W</creatorcontrib><creatorcontrib>Postle, Anthony D</creatorcontrib><title>Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children</title><title>Pediatric critical care medicine</title><addtitle>Pediatr Crit Care Med</addtitle><description><![CDATA[To explore the pathophysiology of acute lung injury in children.
Prospective cohort study.
Regional University Hospital, pediatric intensive care unit.
Children without a preexisting lung injury who developed acute lung injury and were intubated were eligible for the study. Children without lung injury and intubated for minor surgical procedures acted as controls.
Bronchoalveolar lavage fluid and blood were collected on days 1 to 4, weekly, and immediately before extubation during acute lung injury. Molecular species compositions of phosphatidylcholine were determined by electrospray ionization mass spectrometry of lipid extracts of bronchoalveolar lavage fluid supernatants. Surfactant proteins A, B, and D and interleukin-8 were measured in bronchoalveolar lavage fluid and plasma by enzyme-linked immunosorbent assay and Western blotting.
Eighteen children with acute lung injury were enrolled in the study and compared with eight controls. In children with acute lung injury, there were significant changes in the bronchoalveolar lavage fluid phosphatidylcholine species. Bronchoalveolar lavage fluid dipalmitoyl phosphatidylcholine (PC 16:0/16:0) and palmitoyl-myristoyl phosphatidylcholine (PC 16:0/14:0) significantly deceased during acute lung injury (p < .001 and p < .001, respectively), whereas oleoyl-linoleoyl PC (18:1/18:2), palmitoyl-linoleoyl PC (16:0/18:2) and stearoyl-linoleoyl PC (18:0/18:2) characteristic of plasma PC were significantly increased (p < .05, p < .02, and p < .05 respectively), as well as palmitoyl-oleoyl PC (16:0/18:1), and stearoyl-arachidonoyl PC (18:0/20:4) which are characteristic of cell membranes (p < .02, and p < .02, respectively). There were no significant changes to bronchoalveolar lavage fluid, surfactant protein A or B levels compared with controls during acute lung injury, whereas bronchoalveolar lavage fluid, surfactant protein D, and interleukin-8 levels significantly increased (p < .05 and p < .02, respectively). In plasma during acute lung injury, there were significant increases in surfactant proteins A, B, and D, and interleukin-8 (p < .001, p < .001, p < .05, and p < .001, respectively).
Changes to the phosphatidylcholine profile, surfactant proteins, and inflammatory markers of bronchoalveolar lavage fluid and plasma in children with acute lung injury are consistent with an alveolar/blood leakage and inflammatory cell membrane degradation products. These changes are due to alveolar capillary membrane damage and cellular infiltration.]]></description><subject>Acute Lung Injury - physiopathology</subject><subject>Biomarkers - metabolism</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cohort Studies</subject><subject>Female</subject><subject>Humans</subject><subject>Inflammation - diagnosis</subject><subject>Inflammation - physiopathology</subject><subject>Intensive Care Units, Pediatric</subject><subject>Interleukin-8 - metabolism</subject><subject>Intubation, Intratracheal</subject><subject>Lipoproteins - metabolism</subject><subject>Male</subject><subject>Phospholipids - analysis</subject><subject>Prospective Studies</subject><subject>Pulmonary Surfactants - analysis</subject><subject>Respiratory Distress Syndrome, Adult - physiopathology</subject><issn>1529-7535</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdUMtOwzAQ9AFES-EPEPKNCyl-xHZyRBEvqRJIwDlybIe6OE6x40P_HqNWAnFYzWp39jEDwAVGS4xqcfPSNEvUIUwNxRWWhslSHYE5ZqQuBKNsBk5j3CCEa16KEzDDNWOIcjYH7jWFXqpJ-glu12PM4ezW6ngN459OGCdjfS5Kr6H1vZPDIKcx7OAgw6cJEeoUrP-AUqXJQJdyav0mZYL1UK2t08H4M3DcSxfN-QEX4P3-7q15LFbPD0_N7apQlJGpEJzhSnS8I7qmvSxJxYmiWVVZdVQJRrBSpuO8qxQ1UkjOOENaMkm4JqRUdAGu9nvz31_JxKkdbFTGOenNmGIrKK1KVGVYgHLPVGGMMZi-3QabJe1ajNofa9tsbfvf2jx2eTiQusHo36GDr_QbNuB66w</recordid><startdate>201001</startdate><enddate>201001</enddate><creator>Todd, David A</creator><creator>Marsh, Michael J</creator><creator>George, Anne</creator><creator>Henderson, Neil G</creator><creator>Barr, Heather</creator><creator>Sebastian, Seby</creator><creator>Clark, Graeme T</creator><creator>Koster, Grielof</creator><creator>Clark, Howard W</creator><creator>Postle, Anthony D</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201001</creationdate><title>Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children</title><author>Todd, David A ; Marsh, Michael J ; George, Anne ; Henderson, Neil G ; Barr, Heather ; Sebastian, Seby ; Clark, Graeme T ; Koster, Grielof ; Clark, Howard W ; Postle, Anthony D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c352t-765187b6b2d93fa42862c3ae548b3c7521cceb66b8c3ea7a65650da5a26d224c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acute Lung Injury - physiopathology</topic><topic>Biomarkers - metabolism</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cohort Studies</topic><topic>Female</topic><topic>Humans</topic><topic>Inflammation - diagnosis</topic><topic>Inflammation - physiopathology</topic><topic>Intensive Care Units, Pediatric</topic><topic>Interleukin-8 - metabolism</topic><topic>Intubation, Intratracheal</topic><topic>Lipoproteins - metabolism</topic><topic>Male</topic><topic>Phospholipids - analysis</topic><topic>Prospective Studies</topic><topic>Pulmonary Surfactants - analysis</topic><topic>Respiratory Distress Syndrome, Adult - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Todd, David A</creatorcontrib><creatorcontrib>Marsh, Michael J</creatorcontrib><creatorcontrib>George, Anne</creatorcontrib><creatorcontrib>Henderson, Neil G</creatorcontrib><creatorcontrib>Barr, Heather</creatorcontrib><creatorcontrib>Sebastian, Seby</creatorcontrib><creatorcontrib>Clark, Graeme T</creatorcontrib><creatorcontrib>Koster, Grielof</creatorcontrib><creatorcontrib>Clark, Howard W</creatorcontrib><creatorcontrib>Postle, Anthony D</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Pediatric critical care medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Todd, David A</au><au>Marsh, Michael J</au><au>George, Anne</au><au>Henderson, Neil G</au><au>Barr, Heather</au><au>Sebastian, Seby</au><au>Clark, Graeme T</au><au>Koster, Grielof</au><au>Clark, Howard W</au><au>Postle, Anthony D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children</atitle><jtitle>Pediatric critical care medicine</jtitle><addtitle>Pediatr Crit Care Med</addtitle><date>2010-01</date><risdate>2010</risdate><volume>11</volume><issue>1</issue><spage>82</spage><epage>91</epage><pages>82-91</pages><issn>1529-7535</issn><abstract><![CDATA[To explore the pathophysiology of acute lung injury in children.
Prospective cohort study.
Regional University Hospital, pediatric intensive care unit.
Children without a preexisting lung injury who developed acute lung injury and were intubated were eligible for the study. Children without lung injury and intubated for minor surgical procedures acted as controls.
Bronchoalveolar lavage fluid and blood were collected on days 1 to 4, weekly, and immediately before extubation during acute lung injury. Molecular species compositions of phosphatidylcholine were determined by electrospray ionization mass spectrometry of lipid extracts of bronchoalveolar lavage fluid supernatants. Surfactant proteins A, B, and D and interleukin-8 were measured in bronchoalveolar lavage fluid and plasma by enzyme-linked immunosorbent assay and Western blotting.
Eighteen children with acute lung injury were enrolled in the study and compared with eight controls. In children with acute lung injury, there were significant changes in the bronchoalveolar lavage fluid phosphatidylcholine species. Bronchoalveolar lavage fluid dipalmitoyl phosphatidylcholine (PC 16:0/16:0) and palmitoyl-myristoyl phosphatidylcholine (PC 16:0/14:0) significantly deceased during acute lung injury (p < .001 and p < .001, respectively), whereas oleoyl-linoleoyl PC (18:1/18:2), palmitoyl-linoleoyl PC (16:0/18:2) and stearoyl-linoleoyl PC (18:0/18:2) characteristic of plasma PC were significantly increased (p < .05, p < .02, and p < .05 respectively), as well as palmitoyl-oleoyl PC (16:0/18:1), and stearoyl-arachidonoyl PC (18:0/20:4) which are characteristic of cell membranes (p < .02, and p < .02, respectively). There were no significant changes to bronchoalveolar lavage fluid, surfactant protein A or B levels compared with controls during acute lung injury, whereas bronchoalveolar lavage fluid, surfactant protein D, and interleukin-8 levels significantly increased (p < .05 and p < .02, respectively). In plasma during acute lung injury, there were significant increases in surfactant proteins A, B, and D, and interleukin-8 (p < .001, p < .001, p < .05, and p < .001, respectively).
Changes to the phosphatidylcholine profile, surfactant proteins, and inflammatory markers of bronchoalveolar lavage fluid and plasma in children with acute lung injury are consistent with an alveolar/blood leakage and inflammatory cell membrane degradation products. These changes are due to alveolar capillary membrane damage and cellular infiltration.]]></abstract><cop>United States</cop><pmid>19550365</pmid><doi>10.1097/PCC.0b013e3181ae5a4c</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Pediatric critical care medicine, 2010-01, Vol.11 (1), p.82-91 |
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| language | eng |
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| source | Journals@Ovid Ovid Autoload; MEDLINE |
| subjects | Acute Lung Injury - physiopathology Biomarkers - metabolism Bronchoalveolar Lavage Fluid - chemistry Child Child, Preschool Cohort Studies Female Humans Inflammation - diagnosis Inflammation - physiopathology Intensive Care Units, Pediatric Interleukin-8 - metabolism Intubation, Intratracheal Lipoproteins - metabolism Male Phospholipids - analysis Prospective Studies Pulmonary Surfactants - analysis Respiratory Distress Syndrome, Adult - physiopathology |
| title | Surfactant phospholipids, surfactant proteins, and inflammatory markers during acute lung injury in children |
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