Real-time intraoperative fluorescence imaging system using light-absorption correction
We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of...
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| Veröffentlicht in: | Journal of Biomedical Optics 2009-11, Vol.14 (6), p.064012-064019 |
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| container_end_page | 064019 |
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| container_issue | 6 |
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| container_title | Journal of Biomedical Optics |
| container_volume | 14 |
| creator | Themelis, George Yoo, Jung Sun Soh, Kwang-Sup Schulz, Ralf Ntziachristos, Vasilis |
| description | We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras operating in parallel, utilizing a common lens, which allows for the concurrent collection of color, fluorescence, and light attenuation images at the excitation wavelength from the same field of view. The correction is based on a ratio approach of fluorescence over light attenuation images. Color images and video is used for surgical guidance and for registration with the corrected fluorescence images. We showcase the performance metrics of this system on phantoms and animals, and discuss the advantages over conventional epi-illumination systems developed for real-time applications and the limits of validity of corrected epi-illumination fluorescence imaging. |
| doi_str_mv | 10.1117/1.3259362 |
| format | Article |
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We showcase the performance metrics of this system on phantoms and animals, and discuss the advantages over conventional epi-illumination systems developed for real-time applications and the limits of validity of corrected epi-illumination fluorescence imaging.</description><identifier>ISSN: 1083-3668</identifier><identifier>EISSN: 1560-2281</identifier><identifier>DOI: 10.1117/1.3259362</identifier><identifier>PMID: 20059250</identifier><identifier>CODEN: JBOPFO</identifier><language>eng</language><publisher>United States</publisher><subject>Abdomen - anatomy & histology ; Animals ; epi-illumination ; Equipment Design ; fluorescence imaging ; Image Processing, Computer-Assisted - methods ; intraoperative imaging ; Light ; light absorption correction ; light attenuation ; Mice ; Mice, Inbred C57BL ; Phantoms, Imaging ; Spectrometry, Fluorescence - methods ; Time Factors</subject><ispartof>Journal of Biomedical Optics, 2009-11, Vol.14 (6), p.064012-064019</ispartof><rights>2009 Society of Photo-Optical Instrumentation Engineers</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-fa7b36d9d706ecb8ccc4b35292f997d8d5857bf82d15b970cf0e7d41ed62f37e3</citedby><cites>FETCH-LOGICAL-c362t-fa7b36d9d706ecb8ccc4b35292f997d8d5857bf82d15b970cf0e7d41ed62f37e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20059250$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Themelis, George</creatorcontrib><creatorcontrib>Yoo, Jung Sun</creatorcontrib><creatorcontrib>Soh, Kwang-Sup</creatorcontrib><creatorcontrib>Schulz, Ralf</creatorcontrib><creatorcontrib>Ntziachristos, Vasilis</creatorcontrib><title>Real-time intraoperative fluorescence imaging system using light-absorption correction</title><title>Journal of Biomedical Optics</title><addtitle>J Biomed Opt</addtitle><description>We present a novel fluorescence imaging system developed for real-time interventional imaging applications. The system implements a correction scheme that improves the accuracy of epi-illumination fluorescence images for light intensity variation in tissues. The implementation is based on the use of three cameras operating in parallel, utilizing a common lens, which allows for the concurrent collection of color, fluorescence, and light attenuation images at the excitation wavelength from the same field of view. The correction is based on a ratio approach of fluorescence over light attenuation images. Color images and video is used for surgical guidance and for registration with the corrected fluorescence images. 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| ispartof | Journal of Biomedical Optics, 2009-11, Vol.14 (6), p.064012-064019 |
| issn | 1083-3668 1560-2281 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Abdomen - anatomy & histology Animals epi-illumination Equipment Design fluorescence imaging Image Processing, Computer-Assisted - methods intraoperative imaging Light light absorption correction light attenuation Mice Mice, Inbred C57BL Phantoms, Imaging Spectrometry, Fluorescence - methods Time Factors |
| title | Real-time intraoperative fluorescence imaging system using light-absorption correction |
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