Modified graphitized carbon black as transducing material for reagentless H2O2 and enzyme sensors
Direct electron transfer between redox enzymes and electrodes is the basis for the third generation biosensors. We established direct electron transfer between quinohemoprotein alcohol dehydrogenase (PQQ-ADH) and modified carbon black (CBs) electrodes. Furthermore, for the first time, this phenomeno...
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Veröffentlicht in: | Talanta (Oxford) 2005-10, Vol.67 (4), p.783-790 |
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creator | RAZUMIENE, Julija BARKAUSKAS, Jurgis KUBILIUS, Virgaudas MESKYS, Rolandas LAURINAVICIUS, Valdas |
description | Direct electron transfer between redox enzymes and electrodes is the basis for the third generation biosensors. We established direct electron transfer between quinohemoprotein alcohol dehydrogenase (PQQ-ADH) and modified carbon black (CBs) electrodes. Furthermore, for the first time, this phenomenon was observed for pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (PQQ-GDH). Reagentless enzyme biosensors suitable for the determination of ethanol, glucose and sensors for hydrogen peroxide were designed using CB electrodes and screen-printing technique. Aiming to create an optimal transducing material for biosensors, a set of CB batches was synthesized using the matrix of Plackett-Burman experimental design. Depending on the obtained surface functional groups as well as the nano-scale carbon structures in CBs batches, the maximal direct electron transfer current of glucose and ethanol biosensors can vary from 20 to 300 nA and from 30 to 6300 nA for glucose and ethanol, respectively. Using modified CB electrodes, an electrocatalytic oxidation of H(2)O(2) takes place at more negative potentials (0.1-0.4V versus Ag/AgCl). Moreover, H(2)O(2) oxidation efficiency depends on the amount and morphology of fine fraction in the modified CBs. |
doi_str_mv | 10.1016/j.talanta.2005.04.004 |
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We established direct electron transfer between quinohemoprotein alcohol dehydrogenase (PQQ-ADH) and modified carbon black (CBs) electrodes. Furthermore, for the first time, this phenomenon was observed for pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (PQQ-GDH). Reagentless enzyme biosensors suitable for the determination of ethanol, glucose and sensors for hydrogen peroxide were designed using CB electrodes and screen-printing technique. Aiming to create an optimal transducing material for biosensors, a set of CB batches was synthesized using the matrix of Plackett-Burman experimental design. Depending on the obtained surface functional groups as well as the nano-scale carbon structures in CBs batches, the maximal direct electron transfer current of glucose and ethanol biosensors can vary from 20 to 300 nA and from 30 to 6300 nA for glucose and ethanol, respectively. Using modified CB electrodes, an electrocatalytic oxidation of H(2)O(2) takes place at more negative potentials (0.1-0.4V versus Ag/AgCl). Moreover, H(2)O(2) oxidation efficiency depends on the amount and morphology of fine fraction in the modified CBs.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2005.04.004</identifier><identifier>PMID: 18970240</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Biological and medical sciences ; Biosensors ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. 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Using modified CB electrodes, an electrocatalytic oxidation of H(2)O(2) takes place at more negative potentials (0.1-0.4V versus Ag/AgCl). Moreover, H(2)O(2) oxidation efficiency depends on the amount and morphology of fine fraction in the modified CBs.</description><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. 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Technologies</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RAZUMIENE, Julija</creatorcontrib><creatorcontrib>BARKAUSKAS, Jurgis</creatorcontrib><creatorcontrib>KUBILIUS, Virgaudas</creatorcontrib><creatorcontrib>MESKYS, Rolandas</creatorcontrib><creatorcontrib>LAURINAVICIUS, Valdas</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RAZUMIENE, Julija</au><au>BARKAUSKAS, Jurgis</au><au>KUBILIUS, Virgaudas</au><au>MESKYS, Rolandas</au><au>LAURINAVICIUS, Valdas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modified graphitized carbon black as transducing material for reagentless H2O2 and enzyme sensors</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2005-10-15</date><risdate>2005</risdate><volume>67</volume><issue>4</issue><spage>783</spage><epage>790</epage><pages>783-790</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>Direct electron transfer between redox enzymes and electrodes is the basis for the third generation biosensors. We established direct electron transfer between quinohemoprotein alcohol dehydrogenase (PQQ-ADH) and modified carbon black (CBs) electrodes. Furthermore, for the first time, this phenomenon was observed for pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (PQQ-GDH). Reagentless enzyme biosensors suitable for the determination of ethanol, glucose and sensors for hydrogen peroxide were designed using CB electrodes and screen-printing technique. Aiming to create an optimal transducing material for biosensors, a set of CB batches was synthesized using the matrix of Plackett-Burman experimental design. Depending on the obtained surface functional groups as well as the nano-scale carbon structures in CBs batches, the maximal direct electron transfer current of glucose and ethanol biosensors can vary from 20 to 300 nA and from 30 to 6300 nA for glucose and ethanol, respectively. Using modified CB electrodes, an electrocatalytic oxidation of H(2)O(2) takes place at more negative potentials (0.1-0.4V versus Ag/AgCl). Moreover, H(2)O(2) oxidation efficiency depends on the amount and morphology of fine fraction in the modified CBs.</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier</pub><pmid>18970240</pmid><doi>10.1016/j.talanta.2005.04.004</doi><tpages>8</tpages></addata></record> |
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subjects | Biological and medical sciences Biosensors Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Various methods and equipments |
title | Modified graphitized carbon black as transducing material for reagentless H2O2 and enzyme sensors |
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