A mediator-free screen-printed amperometric biosensor for screening of organophosphorus pesticides with flow-injection analysis (FIA) system
A mediator-free amperometric biosensor for screening organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system based on anticholinesterase activity of OPs to immobilized acetylcholinesterase enzyme (AChE) has been developed. The enzyme biosensor is prepared by entrapping AChE in Al 2...
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| Veröffentlicht in: | Talanta (Oxford) 2006-02, Vol.68 (4), p.1089-1095 |
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| creator | Shi, Mianhong Xu, Jingjing Zhang, Song Liu, Baohong Kong, Jilie |
| description | A mediator-free amperometric biosensor for screening organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system based on anticholinesterase activity of OPs to immobilized acetylcholinesterase enzyme (AChE) has been developed. The enzyme biosensor is prepared by entrapping AChE in Al
2O
3 sol–gel matrix screen-printed on an integrated 3-electrode plastic chip. This strategy is found not only increase the stability of the embedded AChE, but also effectively catalyze the oxidative reaction of thiocholine, making the Al
2O
3–AChE biosensor detects the substrate at 0.25
V (versus Ag/AgCl), hundreds mini-volt lower than other reported mediator-free ones. The Al
2O
3–AChE biosensor is thus coupled to FIA system to build up a simple and low-cost FIA–EC system for screening OPs in real samples. A wide linear inhibition response for dichlorvos, typical OP, is observed in the range of 0.1–80
μM, corresponding to 7.91–84.94% inhibition for AChE. The detection limit for dichlorvos is achieved at 10
nM in the simulated seawater for 15
min inhibiting time, which allows the biosensor quantitatively detects the ecotoxicological effect of the real samples from the seaports in eastern China, where the OPs pollution is confirmed by GC–MS. |
| doi_str_mv | 10.1016/j.talanta.2005.07.007 |
| format | Article |
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2O
3 sol–gel matrix screen-printed on an integrated 3-electrode plastic chip. This strategy is found not only increase the stability of the embedded AChE, but also effectively catalyze the oxidative reaction of thiocholine, making the Al
2O
3–AChE biosensor detects the substrate at 0.25
V (versus Ag/AgCl), hundreds mini-volt lower than other reported mediator-free ones. The Al
2O
3–AChE biosensor is thus coupled to FIA system to build up a simple and low-cost FIA–EC system for screening OPs in real samples. A wide linear inhibition response for dichlorvos, typical OP, is observed in the range of 0.1–80
μM, corresponding to 7.91–84.94% inhibition for AChE. The detection limit for dichlorvos is achieved at 10
nM in the simulated seawater for 15
min inhibiting time, which allows the biosensor quantitatively detects the ecotoxicological effect of the real samples from the seaports in eastern China, where the OPs pollution is confirmed by GC–MS.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2005.07.007</identifier><identifier>PMID: 18970436</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Amperometric ; Analytical chemistry ; Applied sciences ; Biological and medical sciences ; Biosensors ; Biotechnology ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Electrochemical methods ; Enzymatic inhibition ; Exact sciences and technology ; Flow-injection analysis ; Fundamental and applied biological sciences. Psychology ; Gas chromatographic methods ; Global environmental pollution ; Mediator-free ; Methods. Procedures. Technologies ; Organophosphorus ; Pollution ; Screen-printed ; Various methods and equipments</subject><ispartof>Talanta (Oxford), 2006-02, Vol.68 (4), p.1089-1095</ispartof><rights>2005 Elsevier B.V.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c394t-3735081abbefda1e2fc491e08e96373e3685e386105b64b3a6463ab0ed4cbaac3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0039914005004248$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17514466$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18970436$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shi, Mianhong</creatorcontrib><creatorcontrib>Xu, Jingjing</creatorcontrib><creatorcontrib>Zhang, Song</creatorcontrib><creatorcontrib>Liu, Baohong</creatorcontrib><creatorcontrib>Kong, Jilie</creatorcontrib><title>A mediator-free screen-printed amperometric biosensor for screening of organophosphorus pesticides with flow-injection analysis (FIA) system</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>A mediator-free amperometric biosensor for screening organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system based on anticholinesterase activity of OPs to immobilized acetylcholinesterase enzyme (AChE) has been developed. The enzyme biosensor is prepared by entrapping AChE in Al
2O
3 sol–gel matrix screen-printed on an integrated 3-electrode plastic chip. This strategy is found not only increase the stability of the embedded AChE, but also effectively catalyze the oxidative reaction of thiocholine, making the Al
2O
3–AChE biosensor detects the substrate at 0.25
V (versus Ag/AgCl), hundreds mini-volt lower than other reported mediator-free ones. The Al
2O
3–AChE biosensor is thus coupled to FIA system to build up a simple and low-cost FIA–EC system for screening OPs in real samples. A wide linear inhibition response for dichlorvos, typical OP, is observed in the range of 0.1–80
μM, corresponding to 7.91–84.94% inhibition for AChE. The detection limit for dichlorvos is achieved at 10
nM in the simulated seawater for 15
min inhibiting time, which allows the biosensor quantitatively detects the ecotoxicological effect of the real samples from the seaports in eastern China, where the OPs pollution is confirmed by GC–MS.</description><subject>Amperometric</subject><subject>Analytical chemistry</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Electrochemical methods</subject><subject>Enzymatic inhibition</subject><subject>Exact sciences and technology</subject><subject>Flow-injection analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gas chromatographic methods</subject><subject>Global environmental pollution</subject><subject>Mediator-free</subject><subject>Methods. Procedures. Technologies</subject><subject>Organophosphorus</subject><subject>Pollution</subject><subject>Screen-printed</subject><subject>Various methods and equipments</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc9u1DAQhy0EotvCI4B8QcAhYbx2_p3QqqKlUiUucLYcZ9J6ldjB46Xad-Ch8Woj9chh7IO_8Yy-H2PvBJQCRP1lXyYzGZ9MuQWoSmhKgOYF24i2kYWsGvmSbQBkV3RCwQW7JNoDwFaCfM0uRNs1oGS9YX93fMbBmRRiMUZETjafvlii8wkHbuYFY5gxRWd57wKhpxD5mOtMOv_Aw8hDfDA-LI-BcsUD8QUpOesGJP7k0iMfp_BUOL9Hm1zw3HgzHckR_3Rzt_vM6UgJ5zfs1WgmwrfrfcV-3Xz7ef29uP9xe3e9uy-s7FQqZCMraIXpexwHI3A7WtUJhBa7Or-hrNsKZVsLqPpa9dLUqpamBxyU7Y2x8op9PP-7xPD7kBfVsyOLU_aJ4UC6kbKV0LVVJqszaWMgijjqLGY28agF6FMOeq_XHPQpBw2NzjnkvvfrhEOf_T53reIz8GEFDFkzjdF46-iZayqhVH3ivp45zD7-OIyarENvc2Yxq9RDcP9Z5R8H1Kyi</recordid><startdate>20060215</startdate><enddate>20060215</enddate><creator>Shi, Mianhong</creator><creator>Xu, Jingjing</creator><creator>Zhang, Song</creator><creator>Liu, Baohong</creator><creator>Kong, Jilie</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060215</creationdate><title>A mediator-free screen-printed amperometric biosensor for screening of organophosphorus pesticides with flow-injection analysis (FIA) system</title><author>Shi, Mianhong ; Xu, Jingjing ; Zhang, Song ; Liu, Baohong ; Kong, Jilie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-3735081abbefda1e2fc491e08e96373e3685e386105b64b3a6463ab0ed4cbaac3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amperometric</topic><topic>Analytical chemistry</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Electrochemical methods</topic><topic>Enzymatic inhibition</topic><topic>Exact sciences and technology</topic><topic>Flow-injection analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gas chromatographic methods</topic><topic>Global environmental pollution</topic><topic>Mediator-free</topic><topic>Methods. Procedures. Technologies</topic><topic>Organophosphorus</topic><topic>Pollution</topic><topic>Screen-printed</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shi, Mianhong</creatorcontrib><creatorcontrib>Xu, Jingjing</creatorcontrib><creatorcontrib>Zhang, Song</creatorcontrib><creatorcontrib>Liu, Baohong</creatorcontrib><creatorcontrib>Kong, Jilie</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shi, Mianhong</au><au>Xu, Jingjing</au><au>Zhang, Song</au><au>Liu, Baohong</au><au>Kong, Jilie</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A mediator-free screen-printed amperometric biosensor for screening of organophosphorus pesticides with flow-injection analysis (FIA) system</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2006-02-15</date><risdate>2006</risdate><volume>68</volume><issue>4</issue><spage>1089</spage><epage>1095</epage><pages>1089-1095</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>A mediator-free amperometric biosensor for screening organophosphorus pesticides (OPs) in flow-injection analysis (FIA) system based on anticholinesterase activity of OPs to immobilized acetylcholinesterase enzyme (AChE) has been developed. The enzyme biosensor is prepared by entrapping AChE in Al
2O
3 sol–gel matrix screen-printed on an integrated 3-electrode plastic chip. This strategy is found not only increase the stability of the embedded AChE, but also effectively catalyze the oxidative reaction of thiocholine, making the Al
2O
3–AChE biosensor detects the substrate at 0.25
V (versus Ag/AgCl), hundreds mini-volt lower than other reported mediator-free ones. The Al
2O
3–AChE biosensor is thus coupled to FIA system to build up a simple and low-cost FIA–EC system for screening OPs in real samples. A wide linear inhibition response for dichlorvos, typical OP, is observed in the range of 0.1–80
μM, corresponding to 7.91–84.94% inhibition for AChE. The detection limit for dichlorvos is achieved at 10
nM in the simulated seawater for 15
min inhibiting time, which allows the biosensor quantitatively detects the ecotoxicological effect of the real samples from the seaports in eastern China, where the OPs pollution is confirmed by GC–MS.</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>18970436</pmid><doi>10.1016/j.talanta.2005.07.007</doi><tpages>7</tpages></addata></record> |
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| subjects | Amperometric Analytical chemistry Applied sciences Biological and medical sciences Biosensors Biotechnology Chemistry Chromatographic methods and physical methods associated with chromatography Electrochemical methods Enzymatic inhibition Exact sciences and technology Flow-injection analysis Fundamental and applied biological sciences. Psychology Gas chromatographic methods Global environmental pollution Mediator-free Methods. Procedures. Technologies Organophosphorus Pollution Screen-printed Various methods and equipments |
| title | A mediator-free screen-printed amperometric biosensor for screening of organophosphorus pesticides with flow-injection analysis (FIA) system |
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