Characterization of proteinaceous glues in old paintings by separation of the o-phtalaldehyde derivatives of their amino acids by liquid chromatography with fluorescence detection
A HPLC-fluorescence method for characterization of proteinaceous glues from binding media used in pictorial works of art prior to conservation or restoration treatment is proposed. Fluorescence derivatization of amino acids released by acid hydrolysis of standard proteins is studied. The derivatizat...
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| Veröffentlicht in: | Talanta (Oxford) 2006-02, Vol.68 (5), p.1648-1654 |
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| container_title | Talanta (Oxford) |
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| creator | Peris-Vicente, J. Gimeno Adelantado, J.V. Carbó, M.T. Doménech Castro, R. Mateo Reig, F. Bosch |
| description | A HPLC-fluorescence method for characterization of proteinaceous glues from binding media used in pictorial works of art prior to conservation or restoration treatment is proposed. Fluorescence derivatization of amino acids released by acid hydrolysis of standard proteins is studied. The derivatization reagent was
o-phtalaldehyde with 2-mercaptoethanol as catalyst. Mobile phase was a programmed gradient among two eluents (water buffered at pH 5.8 wit 5% THF, and methanol) and is able to satisfactorily resolve the amino acid derivatives in 45
min. Peak area ratios among amino acid derivatives and the leucine derivative are useful to characterize the proteins. The method shows good sensitivity and adequate linearity between 2.0
×
10
−3 and 3.3
mmol/l of each amino acid, with a limit of detection of 6.0
×
10
−4
mmol/l. The proposed method has been successfully applied to artistic samples from items of the cultural heritage of Valencia (Spain). |
| doi_str_mv | 10.1016/j.talanta.2005.08.050 |
| format | Article |
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o-phtalaldehyde with 2-mercaptoethanol as catalyst. Mobile phase was a programmed gradient among two eluents (water buffered at pH 5.8 wit 5% THF, and methanol) and is able to satisfactorily resolve the amino acid derivatives in 45
min. Peak area ratios among amino acid derivatives and the leucine derivative are useful to characterize the proteins. The method shows good sensitivity and adequate linearity between 2.0
×
10
−3 and 3.3
mmol/l of each amino acid, with a limit of detection of 6.0
×
10
−4
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o-phtalaldehyde with 2-mercaptoethanol as catalyst. Mobile phase was a programmed gradient among two eluents (water buffered at pH 5.8 wit 5% THF, and methanol) and is able to satisfactorily resolve the amino acid derivatives in 45
min. Peak area ratios among amino acid derivatives and the leucine derivative are useful to characterize the proteins. The method shows good sensitivity and adequate linearity between 2.0
×
10
−3 and 3.3
mmol/l of each amino acid, with a limit of detection of 6.0
×
10
−4
mmol/l. The proposed method has been successfully applied to artistic samples from items of the cultural heritage of Valencia (Spain).</description><subject>Amino acids</subject><subject>Analytical chemistry</subject><subject>Art analysis</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Derivatization</subject><subject>Exact sciences and technology</subject><subject>Fluorescence</subject><subject>HPLC</subject><subject>Other chromatographic methods</subject><subject>Protein</subject><subject>Spectrometric and optical methods</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><recordid>eNqFkc2O1DAQhCMEYoeFRwD5gjgltOMkdk4IjfiTVuICZ6tjOxOPkjhre2Y1vBYviMME7ZGTpfZX1a2qLHtNoaBAm_fHIuKIc8SiBKgLEAXU8CTbUcFZzmrOnmY7ANbmLa3gJnsRwhEASgbseXZDRcuhprDLfu8H9Kii8fYXRutm4nqyeBeNnVEZdwrkMJ5MIDb9jJosaOdo50Mg3YUEsyTxP1UcDHH5Mqx3jdoMF22ITr7nRJyTwxWxnuBkZ0dQWf3XZbT3J6uJGrybMLqDx2W4kAcbB9KPJ-dNUGZWq1c0al32MnvW4xjMq-29zX5-_vRj_zW_-_7l2_7jXa5YW8W8b8qO0rLqOtE1ojKUY9WYsgUuUDS8TGPsFO_KRlUIpuO6EXXXs9ZozloQ7DZ7d_VNedynDKKcbLplTLGvwUjOmGBQUkhkfSWVdyF408vF2wn9RVKQa13yKLe65FqXBCFTXUn3Zttw6iajH1VbPwl4uwEYFI69x1nZ8Mjxqq3rtknchytnUh5na7wMyq6paetTaFI7-59T_gC-zLuG</recordid><startdate>20060228</startdate><enddate>20060228</enddate><creator>Peris-Vicente, J.</creator><creator>Gimeno Adelantado, J.V.</creator><creator>Carbó, M.T. Doménech</creator><creator>Castro, R. Mateo</creator><creator>Reig, F. Bosch</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20060228</creationdate><title>Characterization of proteinaceous glues in old paintings by separation of the o-phtalaldehyde derivatives of their amino acids by liquid chromatography with fluorescence detection</title><author>Peris-Vicente, J. ; Gimeno Adelantado, J.V. ; Carbó, M.T. Doménech ; Castro, R. Mateo ; Reig, F. Bosch</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c394t-f62b1124bb8b684e17a46e29078a8672b8babc7b26c4a0eb7d685bf39ed739083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Amino acids</topic><topic>Analytical chemistry</topic><topic>Art analysis</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Derivatization</topic><topic>Exact sciences and technology</topic><topic>Fluorescence</topic><topic>HPLC</topic><topic>Other chromatographic methods</topic><topic>Protein</topic><topic>Spectrometric and optical methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peris-Vicente, J.</creatorcontrib><creatorcontrib>Gimeno Adelantado, J.V.</creatorcontrib><creatorcontrib>Carbó, M.T. Doménech</creatorcontrib><creatorcontrib>Castro, R. Mateo</creatorcontrib><creatorcontrib>Reig, F. Bosch</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peris-Vicente, J.</au><au>Gimeno Adelantado, J.V.</au><au>Carbó, M.T. Doménech</au><au>Castro, R. Mateo</au><au>Reig, F. Bosch</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of proteinaceous glues in old paintings by separation of the o-phtalaldehyde derivatives of their amino acids by liquid chromatography with fluorescence detection</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2006-02-28</date><risdate>2006</risdate><volume>68</volume><issue>5</issue><spage>1648</spage><epage>1654</epage><pages>1648-1654</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>A HPLC-fluorescence method for characterization of proteinaceous glues from binding media used in pictorial works of art prior to conservation or restoration treatment is proposed. Fluorescence derivatization of amino acids released by acid hydrolysis of standard proteins is studied. The derivatization reagent was
o-phtalaldehyde with 2-mercaptoethanol as catalyst. Mobile phase was a programmed gradient among two eluents (water buffered at pH 5.8 wit 5% THF, and methanol) and is able to satisfactorily resolve the amino acid derivatives in 45
min. Peak area ratios among amino acid derivatives and the leucine derivative are useful to characterize the proteins. The method shows good sensitivity and adequate linearity between 2.0
×
10
−3 and 3.3
mmol/l of each amino acid, with a limit of detection of 6.0
×
10
−4
mmol/l. The proposed method has been successfully applied to artistic samples from items of the cultural heritage of Valencia (Spain).</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>18970510</pmid><doi>10.1016/j.talanta.2005.08.050</doi><tpages>7</tpages></addata></record> |
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| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Amino acids Analytical chemistry Art analysis Chemistry Chromatographic methods and physical methods associated with chromatography Derivatization Exact sciences and technology Fluorescence HPLC Other chromatographic methods Protein Spectrometric and optical methods |
| title | Characterization of proteinaceous glues in old paintings by separation of the o-phtalaldehyde derivatives of their amino acids by liquid chromatography with fluorescence detection |
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