Studies on the chromatographic behavior of nucleosides and bases on p-tert-butyl-calix[8]arene-bonded silica gel stationary phase by HPLC
The chromatographic behavior of some nucleosides, pyrimidines and purines on a new p-tert-butyl-calix[8]arene-bonded silica gel stationary phase (CABS) were studied by high performance liquid chromatography. Their retention behavior on CABS were compared with those on ODS. The influence of mobile ph...
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| Veröffentlicht in: | Talanta (Oxford) 2004-05, Vol.63 (2), p.433-441 |
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| creator | Li, Lai-Sheng Liu, Min Da, Shi-Lu Feng, Yu-Qi |
| description | The chromatographic behavior of some nucleosides, pyrimidines and purines on a new p-tert-butyl-calix[8]arene-bonded silica gel stationary phase (CABS) were studied by high performance liquid chromatography. Their retention behavior on CABS were compared with those on ODS. The influence of mobile phase variables, such as methanol content, pH and ionic strength on the retention behavior were studied. Some nucleosides, pyrimidines and purines on CABS were successfully separated. The results show that the calix[8]arene-bonded phase exhibits high selectivities for the above analytes in high aqueous mobile phases. According to the chromatographic data, it is indicated that hydrophobic interaction, hydrogen-bonding interaction, and dipole–dipole interaction are mainly responsible for the retention behavior. In addition, in some extent, the vertical stacking action of the analytes on CABS can also change the retention behavior. CABS was superior to ODS in the routine fast separation of nucleosides and bases. |
| doi_str_mv | 10.1016/j.talanta.2003.11.016 |
| format | Article |
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Their retention behavior on CABS were compared with those on ODS. The influence of mobile phase variables, such as methanol content, pH and ionic strength on the retention behavior were studied. Some nucleosides, pyrimidines and purines on CABS were successfully separated. The results show that the calix[8]arene-bonded phase exhibits high selectivities for the above analytes in high aqueous mobile phases. According to the chromatographic data, it is indicated that hydrophobic interaction, hydrogen-bonding interaction, and dipole–dipole interaction are mainly responsible for the retention behavior. In addition, in some extent, the vertical stacking action of the analytes on CABS can also change the retention behavior. CABS was superior to ODS in the routine fast separation of nucleosides and bases.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/j.talanta.2003.11.016</identifier><identifier>PMID: 18969451</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical biochemistry: general aspects, technics, instrumentation ; Analytical chemistry ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemistry ; Chromatographic methods and physical methods associated with chromatography ; Exact sciences and technology ; Fundamental and applied biological sciences. 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Their retention behavior on CABS were compared with those on ODS. The influence of mobile phase variables, such as methanol content, pH and ionic strength on the retention behavior were studied. Some nucleosides, pyrimidines and purines on CABS were successfully separated. The results show that the calix[8]arene-bonded phase exhibits high selectivities for the above analytes in high aqueous mobile phases. According to the chromatographic data, it is indicated that hydrophobic interaction, hydrogen-bonding interaction, and dipole–dipole interaction are mainly responsible for the retention behavior. In addition, in some extent, the vertical stacking action of the analytes on CABS can also change the retention behavior. CABS was superior to ODS in the routine fast separation of nucleosides and bases.</description><subject>Analytical biochemistry: general aspects, technics, instrumentation</subject><subject>Analytical chemistry</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Exact sciences and technology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>High performance liquid chromatography</subject><subject>Nucleosides</subject><subject>Other chromatographic methods</subject><subject>p-tert-Butyl-calixarene-bonded silica gel stationary phase</subject><subject>Purines</subject><subject>Pyrimidines</subject><subject>Retention mechanism</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNqFkVGL1DAUhYso7uzqT1DyIj61Jk07aZ9EhtUVBhTUJ5Fwk9xsM3SamqSL8xP812Z3CvvoU-DmO-dezimKV4xWjLLtu0OVYIQpQVVTyivGqjx9UmxYJ3jJW8GfFpv80Zc9a-hFcRnjgVJac8qfFxes67d907JN8fdbWozDSPxE0oBED8EfIfnbAPPgNFE4wJ3zgXhLpkWP6KMzGYfJEAXxLJzLhCGVakmnsdQwuj8_u18QcMJS-cmgIdGNTgO5xZHEBMn5CcKJzEN2IOpEbr7udy-KZxbGiC_X96r48fH6--6m3H_59Hn3YV_qpu1S2bEaBBOitmCtampABUp0ClgP1JqW9YxqrSxvBG96IxoUvG5bhYhbalvLr4q3Z985-N8LxiSPLmocc5jolygF513db0WfyfZM6uBjDGjlHNwxHy4ZlfclyINcS5D3JUjGZJ5m3et1w6KOaB5Va-oZeLMCEHNcNsCkXXzkWkEZfzB6f-Yw53HnMMioHU4ajQuokzTe_eeUf0DSqpk</recordid><startdate>20040528</startdate><enddate>20040528</enddate><creator>Li, Lai-Sheng</creator><creator>Liu, Min</creator><creator>Da, Shi-Lu</creator><creator>Feng, Yu-Qi</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040528</creationdate><title>Studies on the chromatographic behavior of nucleosides and bases on p-tert-butyl-calix[8]arene-bonded silica gel stationary phase by HPLC</title><author>Li, Lai-Sheng ; Liu, Min ; Da, Shi-Lu ; Feng, Yu-Qi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-812a71772faffb42aebab78ba19a0fd51910ccbf347349d74e73255beee60f5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Analytical biochemistry: general aspects, technics, instrumentation</topic><topic>Analytical chemistry</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chemistry</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Exact sciences and technology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>High performance liquid chromatography</topic><topic>Nucleosides</topic><topic>Other chromatographic methods</topic><topic>p-tert-Butyl-calixarene-bonded silica gel stationary phase</topic><topic>Purines</topic><topic>Pyrimidines</topic><topic>Retention mechanism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Lai-Sheng</creatorcontrib><creatorcontrib>Liu, Min</creatorcontrib><creatorcontrib>Da, Shi-Lu</creatorcontrib><creatorcontrib>Feng, Yu-Qi</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Lai-Sheng</au><au>Liu, Min</au><au>Da, Shi-Lu</au><au>Feng, Yu-Qi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Studies on the chromatographic behavior of nucleosides and bases on p-tert-butyl-calix[8]arene-bonded silica gel stationary phase by HPLC</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2004-05-28</date><risdate>2004</risdate><volume>63</volume><issue>2</issue><spage>433</spage><epage>441</epage><pages>433-441</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>The chromatographic behavior of some nucleosides, pyrimidines and purines on a new p-tert-butyl-calix[8]arene-bonded silica gel stationary phase (CABS) were studied by high performance liquid chromatography. Their retention behavior on CABS were compared with those on ODS. The influence of mobile phase variables, such as methanol content, pH and ionic strength on the retention behavior were studied. Some nucleosides, pyrimidines and purines on CABS were successfully separated. The results show that the calix[8]arene-bonded phase exhibits high selectivities for the above analytes in high aqueous mobile phases. According to the chromatographic data, it is indicated that hydrophobic interaction, hydrogen-bonding interaction, and dipole–dipole interaction are mainly responsible for the retention behavior. In addition, in some extent, the vertical stacking action of the analytes on CABS can also change the retention behavior. CABS was superior to ODS in the routine fast separation of nucleosides and bases.</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>18969451</pmid><doi>10.1016/j.talanta.2003.11.016</doi><tpages>9</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 2004-05, Vol.63 (2), p.433-441 |
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| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Analytical biochemistry: general aspects, technics, instrumentation Analytical chemistry Analytical, structural and metabolic biochemistry Biological and medical sciences Chemistry Chromatographic methods and physical methods associated with chromatography Exact sciences and technology Fundamental and applied biological sciences. Psychology High performance liquid chromatography Nucleosides Other chromatographic methods p-tert-Butyl-calixarene-bonded silica gel stationary phase Purines Pyrimidines Retention mechanism |
| title | Studies on the chromatographic behavior of nucleosides and bases on p-tert-butyl-calix[8]arene-bonded silica gel stationary phase by HPLC |
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