A novel amperometric biosensor based on spinach ( Spinacia oleracea) tissue homogenate for urinary oxalate determination
In this study, a novel spinach ( Spinacia oleracea) tissue homogenate-based biosensor for determination of oxalate in urine was developed. The biosensor was constructed by immobilizing tissue homogenate of spinach ( S. oleracea) onto a high-sensitive teflon membrane of a dissolved oxygen (DO) probe....
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| Veröffentlicht in: | Talanta (Oxford) 2003-03, Vol.59 (3), p.545-551 |
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| creator | SEZGINTÜRK, Mustafa Kemal DINCKAYA, Erhan |
| description | In this study, a novel spinach (
Spinacia oleracea) tissue homogenate-based biosensor for determination of oxalate in urine was developed. The biosensor was constructed by immobilizing tissue homogenate of spinach (
S. oleracea) onto a high-sensitive teflon membrane of a dissolved oxygen (DO) probe. For the stability of the biosensor, general immobilization techniques were used to secure the spinach tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of spinach tissue homogenate and gelatin, optimum pH, optimum temperature and thermal stability, interference effects, linear range and repeatability were investigated. A typical calibration curve for the sensor revealed a linear range of 1×10
−5–10×10
−5 M oxalate. In repeatability studies, variation coefficient (CV) was calculated as 1.8%. Of the various substrates tested, only oxalate was found to be specific, with a relative activity of 100%. The method was applied to the determination of oxalate in urine. The results showed that the method was applicable to oxalate determination in urine specifically and selectively. |
| doi_str_mv | 10.1016/S0039-9140(02)00539-8 |
| format | Article |
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Spinacia oleracea) tissue homogenate-based biosensor for determination of oxalate in urine was developed. The biosensor was constructed by immobilizing tissue homogenate of spinach (
S. oleracea) onto a high-sensitive teflon membrane of a dissolved oxygen (DO) probe. For the stability of the biosensor, general immobilization techniques were used to secure the spinach tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of spinach tissue homogenate and gelatin, optimum pH, optimum temperature and thermal stability, interference effects, linear range and repeatability were investigated. A typical calibration curve for the sensor revealed a linear range of 1×10
−5–10×10
−5 M oxalate. In repeatability studies, variation coefficient (CV) was calculated as 1.8%. Of the various substrates tested, only oxalate was found to be specific, with a relative activity of 100%. The method was applied to the determination of oxalate in urine. The results showed that the method was applicable to oxalate determination in urine specifically and selectively.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/S0039-9140(02)00539-8</identifier><identifier>PMID: 18968939</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensors ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Oxalate ; Oxalate biosensor ; Oxalate determination ; Tissue-homogenate biosensor ; Various methods and equipments</subject><ispartof>Talanta (Oxford), 2003-03, Vol.59 (3), p.545-551</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-aef6c0d8215748b351fe8033a1039ade6833bf6ad26b1fb79133dfafb66ebc253</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0039-9140(02)00539-8$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,27906,27907,45977</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14531319$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18968939$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEZGINTÜRK, Mustafa Kemal</creatorcontrib><creatorcontrib>DINCKAYA, Erhan</creatorcontrib><title>A novel amperometric biosensor based on spinach ( Spinacia oleracea) tissue homogenate for urinary oxalate determination</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>In this study, a novel spinach (
Spinacia oleracea) tissue homogenate-based biosensor for determination of oxalate in urine was developed. The biosensor was constructed by immobilizing tissue homogenate of spinach (
S. oleracea) onto a high-sensitive teflon membrane of a dissolved oxygen (DO) probe. For the stability of the biosensor, general immobilization techniques were used to secure the spinach tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of spinach tissue homogenate and gelatin, optimum pH, optimum temperature and thermal stability, interference effects, linear range and repeatability were investigated. A typical calibration curve for the sensor revealed a linear range of 1×10
−5–10×10
−5 M oxalate. In repeatability studies, variation coefficient (CV) was calculated as 1.8%. Of the various substrates tested, only oxalate was found to be specific, with a relative activity of 100%. The method was applied to the determination of oxalate in urine. The results showed that the method was applicable to oxalate determination in urine specifically and selectively.</description><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Oxalate</subject><subject>Oxalate biosensor</subject><subject>Oxalate determination</subject><subject>Tissue-homogenate biosensor</subject><subject>Various methods and equipments</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><recordid>eNqFkE1v1DAQhi0EokvhJ4B8QbSHgJ3ZJM6pWlUUkCpxKJytsT1uXSXxYidV-fd4P9QeOdkePa9n5mHsvRSfpZDtlxshoK96uRZnoj4Xoikv9YKtpOqggqaDl2z1hJywNznfCyFqEPCanUjVt6qHfsUeN3yKDzRwHLeU4khzCpabEDNNOSZuMJPjceJ5Gya0d_yM3-xvAXkcKKElPOdzyHkhfhfHeEsTzsR9yS6pgOkvj4847GqOZkpjqc0hTm_ZK49DpnfH85T9vvr66_J7df3z24_LzXVl142aKyTfWuFULZturQw00pMSACjLbuioVQDGt-jq1khvul4COI_etC0ZWzdwyj4d_t2m-GehPOsxZEvDgBPFJesOQNV93XWFbA6kTTHnRF5vUxjLAloKvXOu9871TqgWtd4716rkPhw7LGYk95w6Si7AxyOA2eLgE0425Gdu3YAEueMuDhwVHw-Bks420GTJhUR21i6G_4zyD7MpoCE</recordid><startdate>20030301</startdate><enddate>20030301</enddate><creator>SEZGINTÜRK, Mustafa Kemal</creator><creator>DINCKAYA, Erhan</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030301</creationdate><title>A novel amperometric biosensor based on spinach ( Spinacia oleracea) tissue homogenate for urinary oxalate determination</title><author>SEZGINTÜRK, Mustafa Kemal ; DINCKAYA, Erhan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-aef6c0d8215748b351fe8033a1039ade6833bf6ad26b1fb79133dfafb66ebc253</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Oxalate</topic><topic>Oxalate biosensor</topic><topic>Oxalate determination</topic><topic>Tissue-homogenate biosensor</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEZGINTÜRK, Mustafa Kemal</creatorcontrib><creatorcontrib>DINCKAYA, Erhan</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEZGINTÜRK, Mustafa Kemal</au><au>DINCKAYA, Erhan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A novel amperometric biosensor based on spinach ( Spinacia oleracea) tissue homogenate for urinary oxalate determination</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>2003-03-01</date><risdate>2003</risdate><volume>59</volume><issue>3</issue><spage>545</spage><epage>551</epage><pages>545-551</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>In this study, a novel spinach (
Spinacia oleracea) tissue homogenate-based biosensor for determination of oxalate in urine was developed. The biosensor was constructed by immobilizing tissue homogenate of spinach (
S. oleracea) onto a high-sensitive teflon membrane of a dissolved oxygen (DO) probe. For the stability of the biosensor, general immobilization techniques were used to secure the spinach tissue homogenate in gelatin-glutaraldehyde cross-linking matrix. In the optimization and characterization studies, the amount of spinach tissue homogenate and gelatin, optimum pH, optimum temperature and thermal stability, interference effects, linear range and repeatability were investigated. A typical calibration curve for the sensor revealed a linear range of 1×10
−5–10×10
−5 M oxalate. In repeatability studies, variation coefficient (CV) was calculated as 1.8%. Of the various substrates tested, only oxalate was found to be specific, with a relative activity of 100%. The method was applied to the determination of oxalate in urine. The results showed that the method was applicable to oxalate determination in urine specifically and selectively.</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>18968939</pmid><doi>10.1016/S0039-9140(02)00539-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Talanta (Oxford), 2003-03, Vol.59 (3), p.545-551 |
| issn | 0039-9140 1873-3573 |
| language | eng |
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| source | Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Biosensors Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Oxalate Oxalate biosensor Oxalate determination Tissue-homogenate biosensor Various methods and equipments |
| title | A novel amperometric biosensor based on spinach ( Spinacia oleracea) tissue homogenate for urinary oxalate determination |
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