Immobilization of lactate oxidase in a poly(vinyl alcohol) matrix on platinized graphite electrodes by chemical cross-linking with isocyanate
A new method for development of an electrochemical sensor based on lactate oxidase is dedbed. Platinized spectroscopic-grade graphite electrodes were modified by chemically cross-linking l-lactate oxidase from Pediococcus species into a poly(vinyl alcohol) network through reaction with a tri-isocyan...
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| Veröffentlicht in: | Talanta (Oxford) 1991, Vol.38 (1), p.37-47 |
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| creator | Hajizadeh, Kiamars Halsall, H.Brian Heineman, William R. |
| description | A new method for development of an electrochemical sensor based on lactate oxidase is dedbed. Platinized spectroscopic-grade graphite electrodes were modified by chemically cross-linking
l-lactate oxidase from
Pediococcus species into a poly(vinyl alcohol) network through reaction with a tri-isocyanate. The immobilized enzyme exhibits high activity and long-term stability. The sensor provides a linear response to
l-lactate over a concentration range of 2 × 10
−5−4 × 10
−3
M and a sensitivity of 1.71 μA.1. mmole
−1. The response time of the sensor is 10–45 sec and the detection limit is 10μ
M. Stable response to the substrate was obtained over a period of 3 months. The new sensor was also used for the analysis of some dairy products without any special pretreatment. |
| doi_str_mv | 10.1016/0039-9140(91)80007-M |
| format | Article |
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l-lactate oxidase from
Pediococcus species into a poly(vinyl alcohol) network through reaction with a tri-isocyanate. The immobilized enzyme exhibits high activity and long-term stability. The sensor provides a linear response to
l-lactate over a concentration range of 2 × 10
−5−4 × 10
−3
M and a sensitivity of 1.71 μA.1. mmole
−1. The response time of the sensor is 10–45 sec and the detection limit is 10μ
M. Stable response to the substrate was obtained over a period of 3 months. The new sensor was also used for the analysis of some dairy products without any special pretreatment.</description><identifier>ISSN: 0039-9140</identifier><identifier>EISSN: 1873-3573</identifier><identifier>DOI: 10.1016/0039-9140(91)80007-M</identifier><identifier>PMID: 18965103</identifier><identifier>CODEN: TLNTA2</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biological and medical sciences ; Biosensors ; Biotechnology ; Fundamental and applied biological sciences. Psychology ; Methods. Procedures. Technologies ; Various methods and equipments</subject><ispartof>Talanta (Oxford), 1991, Vol.38 (1), p.37-47</ispartof><rights>1991</rights><rights>1991 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c389t-b8833c8fe88ba515923ce5abf3438d0abc520fb51873bd02aaa65bc5dcd8425a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0039-9140(91)80007-M$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19425794$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18965103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hajizadeh, Kiamars</creatorcontrib><creatorcontrib>Halsall, H.Brian</creatorcontrib><creatorcontrib>Heineman, William R.</creatorcontrib><title>Immobilization of lactate oxidase in a poly(vinyl alcohol) matrix on platinized graphite electrodes by chemical cross-linking with isocyanate</title><title>Talanta (Oxford)</title><addtitle>Talanta</addtitle><description>A new method for development of an electrochemical sensor based on lactate oxidase is dedbed. Platinized spectroscopic-grade graphite electrodes were modified by chemically cross-linking
l-lactate oxidase from
Pediococcus species into a poly(vinyl alcohol) network through reaction with a tri-isocyanate. The immobilized enzyme exhibits high activity and long-term stability. The sensor provides a linear response to
l-lactate over a concentration range of 2 × 10
−5−4 × 10
−3
M and a sensitivity of 1.71 μA.1. mmole
−1. The response time of the sensor is 10–45 sec and the detection limit is 10μ
M. Stable response to the substrate was obtained over a period of 3 months. The new sensor was also used for the analysis of some dairy products without any special pretreatment.</description><subject>Biological and medical sciences</subject><subject>Biosensors</subject><subject>Biotechnology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Methods. Procedures. Technologies</subject><subject>Various methods and equipments</subject><issn>0039-9140</issn><issn>1873-3573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAURS0EokPhDxDyBtEuAnYcT5wNEqqgrdSKDaytF9vpPHDsYGdK03_gn_F0RnTHxpasc_2uziPkNWfvOePrD4yJrup4w046fqoYY211_YSsuGpFJWQrnpLVP-SIvMj5R2FqwcRzcsRVt5aciRX5czmOsUeP9zBjDDQO1IOZYXY03qGF7CgGCnSKfjm5xbB4Ct7ETfSndIQ54R0tqcmXdMB7Z-lNgmmDJe68M3OK1mXaL9Rs3IgGPDUp5lx5DD8x3NDfOG8o5mgWCGXmS_JsAJ_dq8N9TL5_-fzt7KK6-np-efbpqjJCdXPVKyWEUYNTqgfJZVcL4yT0g2iEsgx6I2s29HLnoresBoC1LI_WWNXUEsQxebf_d0rx19blWY-YjfMegovbrFshVC2F6ArZ7MmH3skNeko4Qlo0Z3q3B72TrHeSy6Ef9qCvS-zNYcC2H519DB3EF-DtAYBctAwJgsH8yHWlZ9s1hfu451zRcYsu6WzQBeMspuJX24j_b_IXeX-n1g</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>Hajizadeh, Kiamars</creator><creator>Halsall, H.Brian</creator><creator>Heineman, William R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1991</creationdate><title>Immobilization of lactate oxidase in a poly(vinyl alcohol) matrix on platinized graphite electrodes by chemical cross-linking with isocyanate</title><author>Hajizadeh, Kiamars ; Halsall, H.Brian ; Heineman, William R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c389t-b8833c8fe88ba515923ce5abf3438d0abc520fb51873bd02aaa65bc5dcd8425a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Biological and medical sciences</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Methods. Procedures. Technologies</topic><topic>Various methods and equipments</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hajizadeh, Kiamars</creatorcontrib><creatorcontrib>Halsall, H.Brian</creatorcontrib><creatorcontrib>Heineman, William R.</creatorcontrib><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Talanta (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hajizadeh, Kiamars</au><au>Halsall, H.Brian</au><au>Heineman, William R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immobilization of lactate oxidase in a poly(vinyl alcohol) matrix on platinized graphite electrodes by chemical cross-linking with isocyanate</atitle><jtitle>Talanta (Oxford)</jtitle><addtitle>Talanta</addtitle><date>1991</date><risdate>1991</risdate><volume>38</volume><issue>1</issue><spage>37</spage><epage>47</epage><pages>37-47</pages><issn>0039-9140</issn><eissn>1873-3573</eissn><coden>TLNTA2</coden><abstract>A new method for development of an electrochemical sensor based on lactate oxidase is dedbed. Platinized spectroscopic-grade graphite electrodes were modified by chemically cross-linking
l-lactate oxidase from
Pediococcus species into a poly(vinyl alcohol) network through reaction with a tri-isocyanate. The immobilized enzyme exhibits high activity and long-term stability. The sensor provides a linear response to
l-lactate over a concentration range of 2 × 10
−5−4 × 10
−3
M and a sensitivity of 1.71 μA.1. mmole
−1. The response time of the sensor is 10–45 sec and the detection limit is 10μ
M. Stable response to the substrate was obtained over a period of 3 months. The new sensor was also used for the analysis of some dairy products without any special pretreatment.</abstract><cop>Amsterdam</cop><cop>Oxford</cop><pub>Elsevier B.V</pub><pmid>18965103</pmid><doi>10.1016/0039-9140(91)80007-M</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0039-9140 |
| ispartof | Talanta (Oxford), 1991, Vol.38 (1), p.37-47 |
| issn | 0039-9140 1873-3573 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | Biological and medical sciences Biosensors Biotechnology Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Various methods and equipments |
| title | Immobilization of lactate oxidase in a poly(vinyl alcohol) matrix on platinized graphite electrodes by chemical cross-linking with isocyanate |
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