Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry
A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (...
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| Veröffentlicht in: | Journal of AOAC International 2010, Vol.93 (1), p.141-149 |
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| creator | SHAH, Hiten J KUNDLIK, Mohan L KAKAD, Abhijit PATEL, Nitesh K PANDYA, Ankit KHATRI, Vanita PRAJAPATI, Shivkumat SUBBAIAH, Gunta PATEL, Chhagan N |
| description | A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (v/v) formic acid in methanol-acetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.5-80.0 ng/mL with r > or = 0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95%. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects. |
| doi_str_mv | 10.1093/jaoac/93.1.141 |
| format | Article |
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The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (v/v) formic acid in methanol-acetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.5-80.0 ng/mL with r > or = 0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95%. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects.</description><identifier>ISSN: 1060-3271</identifier><identifier>EISSN: 1944-7922</identifier><identifier>DOI: 10.1093/jaoac/93.1.141</identifier><identifier>PMID: 20334176</identifier><language>eng</language><publisher>Gaithersburg, MD: AOAC International</publisher><subject>Biological and medical sciences ; Blood Chemical Analysis - methods ; Blood plasma ; Chemical properties ; Chromatography, High Pressure Liquid - methods ; Chromatography, High Pressure Liquid - statistics & numerical data ; Chromatography, Reverse-Phase - methods ; Chromatography, Reverse-Phase - statistics & numerical data ; Composition ; Drug Stability ; Food industries ; Fundamental and applied biological sciences. Psychology ; Humans ; Imipramine - blood ; Imipramine - standards ; Liquid chromatography ; Mass spectrometry ; Methods ; Paroxetine ; Paroxetine - blood ; Physiological aspects ; Reference Standards ; Serotonin Uptake Inhibitors - blood ; Spectrometry, Mass, Electrospray Ionization - methods ; Spectrometry, Mass, Electrospray Ionization - statistics & numerical data ; Tandem Mass Spectrometry - methods ; Tandem Mass Spectrometry - statistics & numerical data ; Technology application</subject><ispartof>Journal of AOAC International, 2010, Vol.93 (1), p.141-149</ispartof><rights>2015 INIST-CNRS</rights><rights>COPYRIGHT 2010 Oxford University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c431t-cb092f18753b6e4ab60f518ba48bb359b9591caf3ccbc5ec1c3b03096a2b504a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22446245$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20334176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHAH, Hiten J</creatorcontrib><creatorcontrib>KUNDLIK, Mohan L</creatorcontrib><creatorcontrib>KAKAD, Abhijit</creatorcontrib><creatorcontrib>PATEL, Nitesh K</creatorcontrib><creatorcontrib>PANDYA, Ankit</creatorcontrib><creatorcontrib>KHATRI, Vanita</creatorcontrib><creatorcontrib>PRAJAPATI, Shivkumat</creatorcontrib><creatorcontrib>SUBBAIAH, Gunta</creatorcontrib><creatorcontrib>PATEL, Chhagan N</creatorcontrib><title>Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry</title><title>Journal of AOAC International</title><addtitle>J AOAC Int</addtitle><description>A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (v/v) formic acid in methanol-acetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.5-80.0 ng/mL with r > or = 0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95%. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects.</description><subject>Biological and medical sciences</subject><subject>Blood Chemical Analysis - methods</subject><subject>Blood plasma</subject><subject>Chemical properties</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Chromatography, High Pressure Liquid - statistics & numerical data</subject><subject>Chromatography, Reverse-Phase - methods</subject><subject>Chromatography, Reverse-Phase - statistics & numerical data</subject><subject>Composition</subject><subject>Drug Stability</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Imipramine - blood</subject><subject>Imipramine - standards</subject><subject>Liquid chromatography</subject><subject>Mass spectrometry</subject><subject>Methods</subject><subject>Paroxetine</subject><subject>Paroxetine - blood</subject><subject>Physiological aspects</subject><subject>Reference Standards</subject><subject>Serotonin Uptake Inhibitors - blood</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>Spectrometry, Mass, Electrospray Ionization - statistics & numerical data</subject><subject>Tandem Mass Spectrometry - methods</subject><subject>Tandem Mass Spectrometry - statistics & numerical data</subject><subject>Technology application</subject><issn>1060-3271</issn><issn>1944-7922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpt0c9vFCEUB_CJ0dhavXo0JMZ4mi2_5gfHZlNtkzXWWM_kwUKXZgamwKSOV_9xaXfVS8OBF_J5L8C3qt4SvCJYsNNbCKBPBVuRFeHkWXVMBOd1Jyh9Xmrc4prRjhxVr1K6xZiTFtOX1RHFjHHStcfV728z-Oys05Bd8ChYdAUx_DTZeYOcRxfzCB5dDZBGQGpBG3c3uy1a72IYIYebCNNuQeswT4PZonuXd-h8MDrHkKYIC7oM3v3az74GvzUj-gIpoe_ToxlNjsvr6oWFIZk3h_2k-vHp_Hp9UW--fr5cn21qzRnJtVZYUEv6rmGqNRxUi21DegW8V4o1QolGEA2Waa10YzTRTGGGRQtUNZgDO6k-7udOMdzNJmU5uqTNMIA3YU6yY6zHom-6It_v5Q0MRjpvQ46gH7Q8oxQTRvuWFbV6QpVVXul08Ma6cv5Ugy6_k6KxcopuhLhIguVDnPIxTlkKIkucpeHd4cazGs32H_-bXwEfDgCShsFG8Nql_45y3lLesD8ACqor</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>SHAH, Hiten J</creator><creator>KUNDLIK, Mohan L</creator><creator>KAKAD, Abhijit</creator><creator>PATEL, Nitesh K</creator><creator>PANDYA, Ankit</creator><creator>KHATRI, Vanita</creator><creator>PRAJAPATI, Shivkumat</creator><creator>SUBBAIAH, Gunta</creator><creator>PATEL, Chhagan N</creator><general>AOAC International</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2010</creationdate><title>Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry</title><author>SHAH, Hiten J ; KUNDLIK, Mohan L ; KAKAD, Abhijit ; PATEL, Nitesh K ; PANDYA, Ankit ; KHATRI, Vanita ; PRAJAPATI, Shivkumat ; SUBBAIAH, Gunta ; PATEL, Chhagan N</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c431t-cb092f18753b6e4ab60f518ba48bb359b9591caf3ccbc5ec1c3b03096a2b504a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Blood Chemical Analysis - methods</topic><topic>Blood plasma</topic><topic>Chemical properties</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Chromatography, High Pressure Liquid - statistics & numerical data</topic><topic>Chromatography, Reverse-Phase - methods</topic><topic>Chromatography, Reverse-Phase - statistics & numerical data</topic><topic>Composition</topic><topic>Drug Stability</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Imipramine - blood</topic><topic>Imipramine - standards</topic><topic>Liquid chromatography</topic><topic>Mass spectrometry</topic><topic>Methods</topic><topic>Paroxetine</topic><topic>Paroxetine - blood</topic><topic>Physiological aspects</topic><topic>Reference Standards</topic><topic>Serotonin Uptake Inhibitors - blood</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>Spectrometry, Mass, Electrospray Ionization - statistics & numerical data</topic><topic>Tandem Mass Spectrometry - methods</topic><topic>Tandem Mass Spectrometry - statistics & numerical data</topic><topic>Technology application</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHAH, Hiten J</creatorcontrib><creatorcontrib>KUNDLIK, Mohan L</creatorcontrib><creatorcontrib>KAKAD, Abhijit</creatorcontrib><creatorcontrib>PATEL, Nitesh K</creatorcontrib><creatorcontrib>PANDYA, Ankit</creatorcontrib><creatorcontrib>KHATRI, Vanita</creatorcontrib><creatorcontrib>PRAJAPATI, Shivkumat</creatorcontrib><creatorcontrib>SUBBAIAH, Gunta</creatorcontrib><creatorcontrib>PATEL, Chhagan N</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of AOAC International</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHAH, Hiten J</au><au>KUNDLIK, Mohan L</au><au>KAKAD, Abhijit</au><au>PATEL, Nitesh K</au><au>PANDYA, Ankit</au><au>KHATRI, Vanita</au><au>PRAJAPATI, Shivkumat</au><au>SUBBAIAH, Gunta</au><au>PATEL, Chhagan N</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry</atitle><jtitle>Journal of AOAC International</jtitle><addtitle>J AOAC Int</addtitle><date>2010</date><risdate>2010</risdate><volume>93</volume><issue>1</issue><spage>141</spage><epage>149</epage><pages>141-149</pages><issn>1060-3271</issn><eissn>1944-7922</eissn><abstract>A rapid LC coupled with electrospray ionization (ESI) MS/MS method was developed and validated for the quantification of paroxetine in heparinized human plasma. The plasma samples were prepared by the solid-phase extraction method without drying or reconstitution. Elution was done with 0.5 mL 0.2% (v/v) formic acid in methanol-acetonitrile (65 + 35, v/v). The analyte and the internal standard (IS; imipramine hydrochloride) were chromatographed on a BDS Hypersil C18 column. The analyte was analyzed by LC/MS/MS with only 1.7 min run time. An ESI interface was chosen for ionization of the analyte from the sample matrix. Selected reaction monitoring mode for detection of paroxetine and the IS were achieved by using m/z 330.17/192.10 and 281.13/86.14, respectively. The LC retention times for paroxetine and imipramine were 0.94 and 1.05 min, respectively. The method was linear in the concentration range of 0.5-80.0 ng/mL with r > or = 0.9995. Recovery of paroxetine and imipramine ranged from 90 to 95%. The assay has been successfully applied to bioequivalence study samples for estimation of paroxetine in healthy human subjects.</abstract><cop>Gaithersburg, MD</cop><pub>AOAC International</pub><pmid>20334176</pmid><doi>10.1093/jaoac/93.1.141</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1060-3271 |
| ispartof | Journal of AOAC International, 2010, Vol.93 (1), p.141-149 |
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| language | eng |
| recordid | cdi_proquest_miscellaneous_733809857 |
| source | MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Biological and medical sciences Blood Chemical Analysis - methods Blood plasma Chemical properties Chromatography, High Pressure Liquid - methods Chromatography, High Pressure Liquid - statistics & numerical data Chromatography, Reverse-Phase - methods Chromatography, Reverse-Phase - statistics & numerical data Composition Drug Stability Food industries Fundamental and applied biological sciences. Psychology Humans Imipramine - blood Imipramine - standards Liquid chromatography Mass spectrometry Methods Paroxetine Paroxetine - blood Physiological aspects Reference Standards Serotonin Uptake Inhibitors - blood Spectrometry, Mass, Electrospray Ionization - methods Spectrometry, Mass, Electrospray Ionization - statistics & numerical data Tandem Mass Spectrometry - methods Tandem Mass Spectrometry - statistics & numerical data Technology application |
| title | Quantification of Paroxetine in Human Plasma by Liquid Chromatography Coupled with Electrospray Ionization Tandem Mass Spectrometry |
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