An expression system for the E. coli fermentation of recombinant antibody Fab fragments from mice and rabbits

A mutually compatible vector system was developed for the bench-top fermenter production of mouse and rabbit Fab fragments comprising pASK85-pro-K411B and pASK85Rab-pro-WR13. These vectors provide a mouse- and rabbit-specific cloning site, respectively, the tetA promoter/operator and the proBA opero...

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Veröffentlicht in:	Journal of AOAC International 2010-01, Vol.93 (1), p.80-88
Hauptverfasser:	Wiebe, Julia C, Schüller, Carolin, Reiche, Jana A, Kramer, Karl, Skerra, Arne, Hock, Bertold
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Base Sequence Cloning, Molecular DNA Primers - genetics Escherichia coli - genetics Escherichia coli - metabolism Fermentation Gene Expression Genetic Vectors Immunoglobulin Fab Fragments - genetics Immunoglobulin Fab Fragments - metabolism Mice Molecular Sequence Data Plasmids - genetics Rabbits Recombinant Proteins - genetics Recombinant Proteins - metabolism
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