Precise DNA Genotyping Diagnosis of Hydatidiform Mole
To estimate whether tissue DNA genotyping is effective for the confirmation and subclassification of hydatidiform moles. Consecutive cases of products of conception were selected based on histologic alterations that are suspicious for molar pregnancy. DNA genotyping was performed by a multiplex poly...
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| Veröffentlicht in: | Obstetrics and gynecology (New York. 1953) 2010-04, Vol.115 (4), p.784-794 |
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| container_title | Obstetrics and gynecology (New York. 1953) |
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| creator | Lipata, Fredilyn Parkash, Vinita Talmor, Monica Bell, Susan Chen, Suping Maric, Vesna Hui, Pei |
| description | To estimate whether tissue DNA genotyping is effective for the confirmation and subclassification of hydatidiform moles.
Consecutive cases of products of conception were selected based on histologic alterations that are suspicious for molar pregnancy. DNA genotyping was performed by a multiplex polymerase chain reaction targeting 15 tetrameric polymorphic loci of the human genome.
A total of 205 products of conception were included. DNA genotyping was informative in all, leading to the final identification of 60 cases of hydatidiform moles, including 17 complete and 43 partial moles. Among 17 cases of complete moles, 14 cases were monospermic and three were dispermic. Forty-three cases were confirmed as triploid partial moles, 42 of which were dispermic and one was monospermic. Among nonmolar cases, 32 gestations showed allelic changes indicating chromosomal alterations, including 28 cases of trisomy syndrome: trisomy 16 (eight cases), trisomy 21 (six cases), trisomy 7 (three cases), trisomy 13 (three cases), trisomy 4 (one case), trisomy 8 (one case), trisomy 18 (one case), XXY/Klinefelter syndrome (one case), and multiple trisomies (four cases). Monosomy 22 was seen in one case. Two nonmolar cases were triploid digynic-monoandric gestations. More complex chromosomal abnormalities were seen in one case. The remaining 113 cases were balanced biallelic gestations.
Tissue DNA genotyping is a practical and highly accurate method for the confirmation and subclassification of hydatidiform moles.
III. |
| doi_str_mv | 10.1097/AOG.0b013e3181d489ec |
| format | Article |
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Consecutive cases of products of conception were selected based on histologic alterations that are suspicious for molar pregnancy. DNA genotyping was performed by a multiplex polymerase chain reaction targeting 15 tetrameric polymorphic loci of the human genome.
A total of 205 products of conception were included. DNA genotyping was informative in all, leading to the final identification of 60 cases of hydatidiform moles, including 17 complete and 43 partial moles. Among 17 cases of complete moles, 14 cases were monospermic and three were dispermic. Forty-three cases were confirmed as triploid partial moles, 42 of which were dispermic and one was monospermic. Among nonmolar cases, 32 gestations showed allelic changes indicating chromosomal alterations, including 28 cases of trisomy syndrome: trisomy 16 (eight cases), trisomy 21 (six cases), trisomy 7 (three cases), trisomy 13 (three cases), trisomy 4 (one case), trisomy 8 (one case), trisomy 18 (one case), XXY/Klinefelter syndrome (one case), and multiple trisomies (four cases). Monosomy 22 was seen in one case. Two nonmolar cases were triploid digynic-monoandric gestations. More complex chromosomal abnormalities were seen in one case. The remaining 113 cases were balanced biallelic gestations.
Tissue DNA genotyping is a practical and highly accurate method for the confirmation and subclassification of hydatidiform moles.
III.</description><identifier>ISSN: 0029-7844</identifier><identifier>EISSN: 1873-233X</identifier><identifier>DOI: 10.1097/AOG.0b013e3181d489ec</identifier><identifier>PMID: 20308840</identifier><identifier>CODEN: OBGNAS</identifier><language>eng</language><publisher>Hagerstown, MD: The American College of Obstetricians and Gynecologists</publisher><subject>Biological and medical sciences ; Chromosome Disorders - diagnosis ; Chromosome Disorders - genetics ; Diseases of mother, fetus and pregnancy ; DNA, Neoplasm - genetics ; Female ; Genotype ; Gynecology. Andrology. Obstetrics ; Humans ; Hydatidiform Mole - classification ; Hydatidiform Mole - diagnosis ; Hydatidiform Mole - genetics ; Hydatidiform Mole - pathology ; Medical sciences ; Polymerase Chain Reaction ; Pregnancy ; Pregnancy. Fetus. Placenta ; Tandem Repeat Sequences ; Uterine Neoplasms - classification ; Uterine Neoplasms - diagnosis ; Uterine Neoplasms - genetics ; Uterine Neoplasms - pathology</subject><ispartof>Obstetrics and gynecology (New York. 1953), 2010-04, Vol.115 (4), p.784-794</ispartof><rights>The American College of Obstetricians and Gynecologists</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3818-462f6861bf1efe38827757b3d658cd2dba2229e8b3dfbc1e79f08689f15fe433</citedby><cites>FETCH-LOGICAL-c3818-462f6861bf1efe38827757b3d658cd2dba2229e8b3dfbc1e79f08689f15fe433</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22555413$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20308840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lipata, Fredilyn</creatorcontrib><creatorcontrib>Parkash, Vinita</creatorcontrib><creatorcontrib>Talmor, Monica</creatorcontrib><creatorcontrib>Bell, Susan</creatorcontrib><creatorcontrib>Chen, Suping</creatorcontrib><creatorcontrib>Maric, Vesna</creatorcontrib><creatorcontrib>Hui, Pei</creatorcontrib><title>Precise DNA Genotyping Diagnosis of Hydatidiform Mole</title><title>Obstetrics and gynecology (New York. 1953)</title><addtitle>Obstet Gynecol</addtitle><description>To estimate whether tissue DNA genotyping is effective for the confirmation and subclassification of hydatidiform moles.
Consecutive cases of products of conception were selected based on histologic alterations that are suspicious for molar pregnancy. DNA genotyping was performed by a multiplex polymerase chain reaction targeting 15 tetrameric polymorphic loci of the human genome.
A total of 205 products of conception were included. DNA genotyping was informative in all, leading to the final identification of 60 cases of hydatidiform moles, including 17 complete and 43 partial moles. Among 17 cases of complete moles, 14 cases were monospermic and three were dispermic. Forty-three cases were confirmed as triploid partial moles, 42 of which were dispermic and one was monospermic. Among nonmolar cases, 32 gestations showed allelic changes indicating chromosomal alterations, including 28 cases of trisomy syndrome: trisomy 16 (eight cases), trisomy 21 (six cases), trisomy 7 (three cases), trisomy 13 (three cases), trisomy 4 (one case), trisomy 8 (one case), trisomy 18 (one case), XXY/Klinefelter syndrome (one case), and multiple trisomies (four cases). Monosomy 22 was seen in one case. Two nonmolar cases were triploid digynic-monoandric gestations. More complex chromosomal abnormalities were seen in one case. The remaining 113 cases were balanced biallelic gestations.
Tissue DNA genotyping is a practical and highly accurate method for the confirmation and subclassification of hydatidiform moles.
III.</description><subject>Biological and medical sciences</subject><subject>Chromosome Disorders - diagnosis</subject><subject>Chromosome Disorders - genetics</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>DNA, Neoplasm - genetics</subject><subject>Female</subject><subject>Genotype</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Hydatidiform Mole - classification</subject><subject>Hydatidiform Mole - diagnosis</subject><subject>Hydatidiform Mole - genetics</subject><subject>Hydatidiform Mole - pathology</subject><subject>Medical sciences</subject><subject>Polymerase Chain Reaction</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Tandem Repeat Sequences</subject><subject>Uterine Neoplasms - classification</subject><subject>Uterine Neoplasms - diagnosis</subject><subject>Uterine Neoplasms - genetics</subject><subject>Uterine Neoplasms - pathology</subject><issn>0029-7844</issn><issn>1873-233X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU1Lw0AQhhdRtH78A5FcxFN09iPZybFYrYJaDx68LZtkVqNptu6mSP-9kVYFT8MMzzsDzzB2zOGcQ6EvxrPpOZTAJUmOvFZYULXFRhy1TIWUz9tsBCCKVKNSe2w_xjcA4Hkhd9meAAmICkYsewxUNZGSycM4mVLn-9Wi6V6SSWNfOh-bmHiX3Kxq2zd143yYJ_e-pUO242wb6WhTD9jT9dXT5U16N5veXo7v0koix1TlwuWY89JxciQRhdaZLmWdZ1jVoi6tEKIgHCaurDjpwgHmWDieOVJSHrCz9dpF8B9Lir2ZN7GitrUd-WU0WkpdoNZ8INWarIKPMZAzi9DMbVgZDuZblxl0mf-6htjJ5sCynFP9G_rxMwCnG8DGyrYu2G6w9ceJLMsUl3_3P33bU4jv7fKTgnkl2_avZhAPucggFcAB1NCl379A-QWjZoI3</recordid><startdate>20100401</startdate><enddate>20100401</enddate><creator>Lipata, Fredilyn</creator><creator>Parkash, Vinita</creator><creator>Talmor, Monica</creator><creator>Bell, Susan</creator><creator>Chen, Suping</creator><creator>Maric, Vesna</creator><creator>Hui, Pei</creator><general>The American College of Obstetricians and Gynecologists</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100401</creationdate><title>Precise DNA Genotyping Diagnosis of Hydatidiform Mole</title><author>Lipata, Fredilyn ; Parkash, Vinita ; Talmor, Monica ; Bell, Susan ; Chen, Suping ; Maric, Vesna ; Hui, Pei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3818-462f6861bf1efe38827757b3d658cd2dba2229e8b3dfbc1e79f08689f15fe433</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Biological and medical sciences</topic><topic>Chromosome Disorders - diagnosis</topic><topic>Chromosome Disorders - genetics</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>DNA, Neoplasm - genetics</topic><topic>Female</topic><topic>Genotype</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Hydatidiform Mole - classification</topic><topic>Hydatidiform Mole - diagnosis</topic><topic>Hydatidiform Mole - genetics</topic><topic>Hydatidiform Mole - pathology</topic><topic>Medical sciences</topic><topic>Polymerase Chain Reaction</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Tandem Repeat Sequences</topic><topic>Uterine Neoplasms - classification</topic><topic>Uterine Neoplasms - diagnosis</topic><topic>Uterine Neoplasms - genetics</topic><topic>Uterine Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lipata, Fredilyn</creatorcontrib><creatorcontrib>Parkash, Vinita</creatorcontrib><creatorcontrib>Talmor, Monica</creatorcontrib><creatorcontrib>Bell, Susan</creatorcontrib><creatorcontrib>Chen, Suping</creatorcontrib><creatorcontrib>Maric, Vesna</creatorcontrib><creatorcontrib>Hui, Pei</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lipata, Fredilyn</au><au>Parkash, Vinita</au><au>Talmor, Monica</au><au>Bell, Susan</au><au>Chen, Suping</au><au>Maric, Vesna</au><au>Hui, Pei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Precise DNA Genotyping Diagnosis of Hydatidiform Mole</atitle><jtitle>Obstetrics and gynecology (New York. 1953)</jtitle><addtitle>Obstet Gynecol</addtitle><date>2010-04-01</date><risdate>2010</risdate><volume>115</volume><issue>4</issue><spage>784</spage><epage>794</epage><pages>784-794</pages><issn>0029-7844</issn><eissn>1873-233X</eissn><coden>OBGNAS</coden><abstract>To estimate whether tissue DNA genotyping is effective for the confirmation and subclassification of hydatidiform moles.
Consecutive cases of products of conception were selected based on histologic alterations that are suspicious for molar pregnancy. DNA genotyping was performed by a multiplex polymerase chain reaction targeting 15 tetrameric polymorphic loci of the human genome.
A total of 205 products of conception were included. DNA genotyping was informative in all, leading to the final identification of 60 cases of hydatidiform moles, including 17 complete and 43 partial moles. Among 17 cases of complete moles, 14 cases were monospermic and three were dispermic. Forty-three cases were confirmed as triploid partial moles, 42 of which were dispermic and one was monospermic. Among nonmolar cases, 32 gestations showed allelic changes indicating chromosomal alterations, including 28 cases of trisomy syndrome: trisomy 16 (eight cases), trisomy 21 (six cases), trisomy 7 (three cases), trisomy 13 (three cases), trisomy 4 (one case), trisomy 8 (one case), trisomy 18 (one case), XXY/Klinefelter syndrome (one case), and multiple trisomies (four cases). Monosomy 22 was seen in one case. Two nonmolar cases were triploid digynic-monoandric gestations. More complex chromosomal abnormalities were seen in one case. The remaining 113 cases were balanced biallelic gestations.
Tissue DNA genotyping is a practical and highly accurate method for the confirmation and subclassification of hydatidiform moles.
III.</abstract><cop>Hagerstown, MD</cop><pub>The American College of Obstetricians and Gynecologists</pub><pmid>20308840</pmid><doi>10.1097/AOG.0b013e3181d489ec</doi><tpages>11</tpages></addata></record> |
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| subjects | Biological and medical sciences Chromosome Disorders - diagnosis Chromosome Disorders - genetics Diseases of mother, fetus and pregnancy DNA, Neoplasm - genetics Female Genotype Gynecology. Andrology. Obstetrics Humans Hydatidiform Mole - classification Hydatidiform Mole - diagnosis Hydatidiform Mole - genetics Hydatidiform Mole - pathology Medical sciences Polymerase Chain Reaction Pregnancy Pregnancy. Fetus. Placenta Tandem Repeat Sequences Uterine Neoplasms - classification Uterine Neoplasms - diagnosis Uterine Neoplasms - genetics Uterine Neoplasms - pathology |
| title | Precise DNA Genotyping Diagnosis of Hydatidiform Mole |
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