Purification and characterization of the ouabain-sensitive H +/K +-ATPase from guinea-pig distal colon

Distal colon absorbs K + through a Na +-independent, ouabain-sensitive H +/K +-exchange, associated to an apical ouabain-sensitive H +/K +-ATPase. Expression of HKα2, gene associated with this ATPase, induces K +-transport mechanisms, whose ouabain susceptibility is inconsistent. Both ouabain-sensit...

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Veröffentlicht in:	Archives of biochemistry and biophysics 2010-04, Vol.496 (1), p.21-32
Hauptverfasser:	Belisario, Dimas C., Rocafull, Miguel A., del Castillo, Jesús R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Cell Membrane - metabolism Cell Polarity Colon - cytology Colon - enzymology Electrophoresis, Polyacrylamide Gel Gene Expression Regulation, Enzymologic Guinea Pigs H(+)-K(+)-Exchanging ATPase - chemistry H(+)-K(+)-Exchanging ATPase - genetics H(+)-K(+)-Exchanging ATPase - isolation & purification H(+)-K(+)-Exchanging ATPase - metabolism HKα2 gene Isoenzymes - chemistry Isoenzymes - genetics Isoenzymes - isolation & purification Isoenzymes - metabolism Male Mammalian distal colon Molecular Sequence Data Mucous Membrane - cytology Na +/K +-ATPase β1-subunit Ouabain - metabolism Phosphoproteins - metabolism Phosphorylation Potassium absorption Rats Reverse Transcriptase Polymerase Chain Reaction Sodium - metabolism Tandem Mass Spectrometry
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