Phospholipid transverse mobility modifications in plasma membranes of activated platelets: An ESR study
Spin labeled phospholipid analogs were used to directly study changes in aminophospholipid translocase activity in activated platelets. In thrombin-activated platelets, the translocase activity was slightly stimulated, whereas no vesicle formation or proteolysis of cytoskeletal protein occurred. Ca...
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| Veröffentlicht in: | Biochemical and biophysical research communications 1992-11, Vol.189 (1), p.465-471 |
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| creator | Bassé, François Gaffet, Patrick Rendu, Francine Bienvenüe, Alain |
| description | Spin labeled phospholipid analogs were used to directly study changes in aminophospholipid translocase activity in activated platelets. In thrombin-activated platelets, the translocase activity was slightly stimulated, whereas no vesicle formation or proteolysis of cytoskeletal protein occurred. Ca
2+ ionophore A23187-mediated activation produced vesiculation and proteolysis. Additionally, the translocase activity was completely inhibited, probably due to a sharp rise the intracellular Ca
2+ concentration, as shown when platelets were activated in the presence of various A23187 and Ca
2+ concentrations and by the recovery of the translocase activity when Ca
2+ was complexed with EGTA. No translocase activity was found in vesicles. Whereas vesiculation and translocase inhibition can occur independently of proteolysis, this later accentuated the shedding phenomenon. |
| doi_str_mv | 10.1016/0006-291X(92)91581-A |
| format | Article |
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2+ ionophore A23187-mediated activation produced vesiculation and proteolysis. Additionally, the translocase activity was completely inhibited, probably due to a sharp rise the intracellular Ca
2+ concentration, as shown when platelets were activated in the presence of various A23187 and Ca
2+ concentrations and by the recovery of the translocase activity when Ca
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2+ ionophore A23187-mediated activation produced vesiculation and proteolysis. Additionally, the translocase activity was completely inhibited, probably due to a sharp rise the intracellular Ca
2+ concentration, as shown when platelets were activated in the presence of various A23187 and Ca
2+ concentrations and by the recovery of the translocase activity when Ca
2+ was complexed with EGTA. No translocase activity was found in vesicles. Whereas vesiculation and translocase inhibition can occur independently of proteolysis, this later accentuated the shedding phenomenon.</description><subject>Biological and medical sciences</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood Platelets - drug effects</subject><subject>Blood Platelets - metabolism</subject><subject>Calcimycin - pharmacology</subject><subject>Calcium - pharmacology</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Egtazic Acid - pharmacology</subject><subject>Electron Spin Resonance Spectroscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Kinetics</subject><subject>Molecular and cellular biology</subject><subject>Phospholipids - blood</subject><subject>Platelet</subject><subject>Platelet Activation - drug effects</subject><subject>Spin Labels</subject><subject>Thrombin - pharmacology</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFDEUhoModdv6DxRyIaIXY5NJJpl4ISylVqGg-AHehUxyYiPz1TnZhf33zbpLe-fVOfA-7-HwEPKSs_eccXXBGFNVbfjvt6Z-Z3jT8mr9hKw4M6yqOZNPyeoBeU5OEf8yxrlU5oSccCEEN82K_Pl2O-F8O_VpToHmxY24hQWBDlOX-pR3ZQkpJu9ymkakaaRz73BwdIChKzggnSJ1PqetyxD2aYYeMn6g65Fe_fhOMW_C7pw8i65HeHGcZ-TXp6ufl5-rm6_XXy7XN5UXrcpVVEHFNkgwGmJUJmqvZO3aGhotamm0dg1njesCd9rVWjloWuVaHlthYgfijLw53J2X6W4DmO2Q0EPfl0-nDVothNZaNgWUB9AvE-IC0c5LGtyys5zZvV-7l2f38qyp7T-_dl1qr473N90A4bF0EFry18fcoXd9LIZ8wgdMSqNa2Rbs4wGD4mKbYLHoE4weQlrAZxum9P8_7gGybpi0</recordid><startdate>19921130</startdate><enddate>19921130</enddate><creator>Bassé, François</creator><creator>Gaffet, Patrick</creator><creator>Rendu, Francine</creator><creator>Bienvenüe, Alain</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921130</creationdate><title>Phospholipid transverse mobility modifications in plasma membranes of activated platelets: An ESR study</title><author>Bassé, François ; Gaffet, Patrick ; Rendu, Francine ; Bienvenüe, Alain</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-f6d6f8d4e97eff69f7c642a82e57324977a5105abd1a7a276ae586a81f839fbe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood Platelets - drug effects</topic><topic>Blood Platelets - metabolism</topic><topic>Calcimycin - pharmacology</topic><topic>Calcium - pharmacology</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Egtazic Acid - pharmacology</topic><topic>Electron Spin Resonance Spectroscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Kinetics</topic><topic>Molecular and cellular biology</topic><topic>Phospholipids - blood</topic><topic>Platelet</topic><topic>Platelet Activation - drug effects</topic><topic>Spin Labels</topic><topic>Thrombin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bassé, François</creatorcontrib><creatorcontrib>Gaffet, Patrick</creatorcontrib><creatorcontrib>Rendu, Francine</creatorcontrib><creatorcontrib>Bienvenüe, Alain</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bassé, François</au><au>Gaffet, Patrick</au><au>Rendu, Francine</au><au>Bienvenüe, Alain</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phospholipid transverse mobility modifications in plasma membranes of activated platelets: An ESR study</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>1992-11-30</date><risdate>1992</risdate><volume>189</volume><issue>1</issue><spage>465</spage><epage>471</epage><pages>465-471</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><coden>BBRCA9</coden><abstract>Spin labeled phospholipid analogs were used to directly study changes in aminophospholipid translocase activity in activated platelets. In thrombin-activated platelets, the translocase activity was slightly stimulated, whereas no vesicle formation or proteolysis of cytoskeletal protein occurred. Ca
2+ ionophore A23187-mediated activation produced vesiculation and proteolysis. Additionally, the translocase activity was completely inhibited, probably due to a sharp rise the intracellular Ca
2+ concentration, as shown when platelets were activated in the presence of various A23187 and Ca
2+ concentrations and by the recovery of the translocase activity when Ca
2+ was complexed with EGTA. No translocase activity was found in vesicles. Whereas vesiculation and translocase inhibition can occur independently of proteolysis, this later accentuated the shedding phenomenon.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1333195</pmid><doi>10.1016/0006-291X(92)91581-A</doi><tpages>7</tpages></addata></record> |
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| ispartof | Biochemical and biophysical research communications, 1992-11, Vol.189 (1), p.465-471 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Blood coagulation. Blood cells Blood Platelets - drug effects Blood Platelets - metabolism Calcimycin - pharmacology Calcium - pharmacology Cell Membrane - drug effects Cell Membrane - metabolism Egtazic Acid - pharmacology Electron Spin Resonance Spectroscopy Fundamental and applied biological sciences. Psychology Humans In Vitro Techniques Kinetics Molecular and cellular biology Phospholipids - blood Platelet Platelet Activation - drug effects Spin Labels Thrombin - pharmacology |
| title | Phospholipid transverse mobility modifications in plasma membranes of activated platelets: An ESR study |
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