Trimerization of the heat shock transcription factor by a triple-stranded .alpha.-helical coiled-coil
We have isolated and characterized a 91 amino acid fragment of the heat shock transcription factor from both Saccharomyces cerevisiae and Kluyveromyces lactis. The two protein fragments behave similarly: they form homotrimers, as indicated by sedimentation equilibrium and cross-linking, and contain...
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| Veröffentlicht in: | Biochemistry (Easton) 1992-12, Vol.31 (48), p.12272-12276 |
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| container_end_page | 12276 |
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| container_title | Biochemistry (Easton) |
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| creator | Peteranderl, Ralph Nelson, Hillary C. M |
| description | We have isolated and characterized a 91 amino acid fragment of the heat shock transcription factor from both Saccharomyces cerevisiae and Kluyveromyces lactis. The two protein fragments behave similarly: they form homotrimers, as indicated by sedimentation equilibrium and cross-linking, and contain approximately 80% alpha-helix, as indicated by circular dichroism. Sedimentation velocity and diffusion coefficients indicate that they have an elongated, nonspherical shape. We conclude the following: these fragments contain a domain which forms a trimer via a triple-stranded alpha-helical coiled-coil, similar to that found in influenza hemagglutinin |
| doi_str_mv | 10.1021/bi00163a042 |
| format | Article |
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We conclude the following: these fragments contain a domain which forms a trimer via a triple-stranded alpha-helical coiled-coil, similar to that found in influenza hemagglutinin</description><identifier>ISSN: 0006-2960</identifier><identifier>EISSN: 1520-4995</identifier><identifier>DOI: 10.1021/bi00163a042</identifier><identifier>PMID: 1457424</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>ADN ; Amino Acid Sequence ; Analytical, structural and metabolic biochemistry ; Binding and carrier proteins ; Biological and medical sciences ; Circular Dichroism ; Cloning, Molecular ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - genetics ; Electrophoresis, Polyacrylamide Gel ; Escherichia coli ; ESTRES TERMICO ; Fundamental and applied biological sciences. 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M</creatorcontrib><title>Trimerization of the heat shock transcription factor by a triple-stranded .alpha.-helical coiled-coil</title><title>Biochemistry (Easton)</title><addtitle>Biochemistry</addtitle><description>We have isolated and characterized a 91 amino acid fragment of the heat shock transcription factor from both Saccharomyces cerevisiae and Kluyveromyces lactis. The two protein fragments behave similarly: they form homotrimers, as indicated by sedimentation equilibrium and cross-linking, and contain approximately 80% alpha-helix, as indicated by circular dichroism. Sedimentation velocity and diffusion coefficients indicate that they have an elongated, nonspherical shape. We conclude the following: these fragments contain a domain which forms a trimer via a triple-stranded alpha-helical coiled-coil, similar to that found in influenza hemagglutinin</description><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Binding and carrier proteins</subject><subject>Biological and medical sciences</subject><subject>Circular Dichroism</subject><subject>Cloning, Molecular</subject><subject>DNA-Binding Proteins - chemistry</subject><subject>DNA-Binding Proteins - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Escherichia coli</subject><subject>ESTRES TERMICO</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heat Shock Transcription Factors</subject><subject>Kluyveromyces - chemistry</subject><subject>Kluyveromyces lactis</subject><subject>KLUYVEROMYCES MARXIANUS</subject><subject>Light</subject><subject>Molecular Sequence Data</subject><subject>Peptide Fragments - chemistry</subject><subject>Protein Conformation</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Proteins</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - chemistry</subject><subject>Scattering, Radiation</subject><subject>Sequence Homology, Amino Acid</subject><subject>STRESS THERMIQUE</subject><subject>Transcription Factors - chemistry</subject><subject>Transcription Factors - genetics</subject><issn>0006-2960</issn><issn>1520-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1rFEEQhhtR4mb15E0Q5iB6kFn7u2eOkmgUAoq7OTc1PTVOJ7Pbk-5ZMPn19jpL9CB4Korn4aV4i5AXjK4Y5ex94yllWgCV_BFZMMVpKetaPSYLSqkuea3pU3Ka0nVeJTXyhJwwqYzkckFwE_0Wo7-HyYddEbpi6rHoEaYi9cHdFFOEXXLRj795B24KsWjuCsjEjwOW6WC02BYrGMYeVmWPg3cwFC74AdvyMJ6RJx0MCZ8f55Jcffq4OftcXn69-HL24bKEfPBUVpV0CNQwZUwjjQZouRJtDdhio5vGcONaKlSlkSvGWcWbTmCrXS1r1QktluTNnDvGcLvHNNmtTw6HAXYY9skaIYzO8n9FpjXXIutL8m4WXQwpRezsmAuDeGcZtYf27V_tZ_vVMXbfbLH94851Z_76yCHlirrcnPPpQZOyqquKZq2cNZ8m_PmAId5YbYRRdvNtbXl1UVfn39f2PPsvZ7-DYOFHzJFX61rl54tD2NsZgkv2OuzjLn_gn9f_Apmqr7Q</recordid><startdate>19921208</startdate><enddate>19921208</enddate><creator>Peteranderl, Ralph</creator><creator>Nelson, Hillary C. 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Psychology</topic><topic>Heat Shock Transcription Factors</topic><topic>Kluyveromyces - chemistry</topic><topic>Kluyveromyces lactis</topic><topic>KLUYVEROMYCES MARXIANUS</topic><topic>Light</topic><topic>Molecular Sequence Data</topic><topic>Peptide Fragments - chemistry</topic><topic>Protein Conformation</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Proteins</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - chemistry</topic><topic>Scattering, Radiation</topic><topic>Sequence Homology, Amino Acid</topic><topic>STRESS THERMIQUE</topic><topic>Transcription Factors - chemistry</topic><topic>Transcription Factors - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peteranderl, Ralph</creatorcontrib><creatorcontrib>Nelson, Hillary C. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Trimerization of the heat shock transcription factor by a triple-stranded .alpha.-helical coiled-coil</atitle><jtitle>Biochemistry (Easton)</jtitle><addtitle>Biochemistry</addtitle><date>1992-12-08</date><risdate>1992</risdate><volume>31</volume><issue>48</issue><spage>12272</spage><epage>12276</epage><pages>12272-12276</pages><issn>0006-2960</issn><eissn>1520-4995</eissn><abstract>We have isolated and characterized a 91 amino acid fragment of the heat shock transcription factor from both Saccharomyces cerevisiae and Kluyveromyces lactis. The two protein fragments behave similarly: they form homotrimers, as indicated by sedimentation equilibrium and cross-linking, and contain approximately 80% alpha-helix, as indicated by circular dichroism. Sedimentation velocity and diffusion coefficients indicate that they have an elongated, nonspherical shape. We conclude the following: these fragments contain a domain which forms a trimer via a triple-stranded alpha-helical coiled-coil, similar to that found in influenza hemagglutinin</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>1457424</pmid><doi>10.1021/bi00163a042</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochemistry (Easton), 1992-12, Vol.31 (48), p.12272-12276 |
| issn | 0006-2960 1520-4995 |
| language | eng |
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| source | ACS Publications; MEDLINE |
| subjects | ADN Amino Acid Sequence Analytical, structural and metabolic biochemistry Binding and carrier proteins Biological and medical sciences Circular Dichroism Cloning, Molecular DNA-Binding Proteins - chemistry DNA-Binding Proteins - genetics Electrophoresis, Polyacrylamide Gel Escherichia coli ESTRES TERMICO Fundamental and applied biological sciences. Psychology Heat Shock Transcription Factors Kluyveromyces - chemistry Kluyveromyces lactis KLUYVEROMYCES MARXIANUS Light Molecular Sequence Data Peptide Fragments - chemistry Protein Conformation PROTEINAS PROTEINE Proteins SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - chemistry Scattering, Radiation Sequence Homology, Amino Acid STRESS THERMIQUE Transcription Factors - chemistry Transcription Factors - genetics |
| title | Trimerization of the heat shock transcription factor by a triple-stranded .alpha.-helical coiled-coil |
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