Detection of Brucella spp. in soft cheese by semi-nested polymerase chain reaction
Brucellae are Gram-negative bacteria with a predilection for the reticuloendothelial system and the reproductive tract of cattle, sheep, goats and pigs. In these species brucellosis is of great economic significance because of the resulting abortion and infertility (Samartino et al. 1993; Corbel, 19...
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| Veröffentlicht in: | Journal of dairy research 2003-05, Vol.70 (2), p.245-247 |
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| creator | Tantillo, Giuseppina M Di Pinto, Angela Buonavoglia, Canio |
| description | Brucellae are Gram-negative bacteria with a predilection for the reticuloendothelial system and the reproductive tract of cattle, sheep, goats and pigs. In these species brucellosis is of great economic significance because of the resulting abortion and infertility (Samartino et al. 1993; Corbel, 1997; Altecruse et al. 1998). Human brucellosis, caused by contamination from infected animals (percutaneous, conjunctival or nasal infection), is very common and remains a serious health hazard in Mediterranean Europe, western Asia, and parts of Africa and South America (Boschiroli et al. 2001). Raw milk, butter, ice-cream and unpasteurized cheese are also potential vehicles for the transmission of Brucella. |
| doi_str_mv | 10.1017/S0022029903006071 |
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In these species brucellosis is of great economic significance because of the resulting abortion and infertility (Samartino et al. 1993; Corbel, 1997; Altecruse et al. 1998). Human brucellosis, caused by contamination from infected animals (percutaneous, conjunctival or nasal infection), is very common and remains a serious health hazard in Mediterranean Europe, western Asia, and parts of Africa and South America (Boschiroli et al. 2001). Raw milk, butter, ice-cream and unpasteurized cheese are also potential vehicles for the transmission of Brucella.</description><identifier>ISSN: 0022-0299</identifier><identifier>EISSN: 1469-7629</identifier><identifier>DOI: 10.1017/S0022029903006071</identifier><identifier>PMID: 12800881</identifier><identifier>CODEN: JDRSAN</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Bacteria ; Biological and medical sciences ; Brucella - genetics ; Brucella - isolation & purification ; Brucella spp ; Cheese - microbiology ; Dairy products ; DNA, Bacterial - analysis ; Food industries ; Food microbiology ; Fundamental and applied biological sciences. Psychology ; Health hazards ; Infertility ; Milk and cheese industries. 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In these species brucellosis is of great economic significance because of the resulting abortion and infertility (Samartino et al. 1993; Corbel, 1997; Altecruse et al. 1998). Human brucellosis, caused by contamination from infected animals (percutaneous, conjunctival or nasal infection), is very common and remains a serious health hazard in Mediterranean Europe, western Asia, and parts of Africa and South America (Boschiroli et al. 2001). Raw milk, butter, ice-cream and unpasteurized cheese are also potential vehicles for the transmission of Brucella.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Brucella - genetics</subject><subject>Brucella - isolation & purification</subject><subject>Brucella spp</subject><subject>Cheese - microbiology</subject><subject>Dairy products</subject><subject>DNA, Bacterial - analysis</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Health hazards</subject><subject>Infertility</subject><subject>Milk and cheese industries. Ice creams</subject><subject>Polymerase Chain Reaction - methods</subject><subject>semi-nested PCR</subject><subject>Sensitivity and Specificity</subject><subject>soft cheeses</subject><issn>0022-0299</issn><issn>1469-7629</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kM1v1DAQxS1ERbeFP4ALipDoLWVsJ_440sKWokqIdhFHy3HGNCVf2InE_vd4uxErgTjN4f3mzZtHyEsK5xSofHsHwBgwrYEDCJD0CVnRQuhcCqafktVOznf6MTmJ8QGActDiGTmmTAEoRVfk9j1O6KZm6LPBZxdhdti2NovjeJ41fRYHP2XuHjFiVm2ziF2T9xgnrLNxaLcdBpsUd28TG9A-Gj0nR962EV8s85R8XX_YXH7Mbz5fXV--u8ldwcWUe1lWXEhUiiFypp2ooLCFq4WWzFHUqpRF7aVQsuSspmAVrbwqpNYehFb8lJztfccw_JxTKNM18TF-j8McjeQ89aBEAl__BT4Mc-hTNsNYsuNQ7tzoHnJhiDGgN2NoOhu2hoLZtW3-aTvtvFqM56rD-rCx1JuANwtgo7OtD7Z3TTxwhSzF_ni-55pU7a8_ug0_jJBclkZcfTGbtdLfNutPZpN4voS1XRWa-jseXvp_3N86qKMx</recordid><startdate>20030501</startdate><enddate>20030501</enddate><creator>Tantillo, Giuseppina M</creator><creator>Di Pinto, Angela</creator><creator>Buonavoglia, Canio</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7S</scope><scope>P64</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>7X8</scope></search><sort><creationdate>20030501</creationdate><title>Detection of Brucella spp. in soft cheese by semi-nested polymerase chain reaction</title><author>Tantillo, Giuseppina M ; Di Pinto, Angela ; Buonavoglia, Canio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c436t-f75b367e882ee329c6b04a4cd6972c1e98574df7687532d10a81bf84799f06983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Brucella - genetics</topic><topic>Brucella - isolation & purification</topic><topic>Brucella spp</topic><topic>Cheese - microbiology</topic><topic>Dairy products</topic><topic>DNA, Bacterial - analysis</topic><topic>Food industries</topic><topic>Food microbiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Health hazards</topic><topic>Infertility</topic><topic>Milk and cheese industries. 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| subjects | Animals Bacteria Biological and medical sciences Brucella - genetics Brucella - isolation & purification Brucella spp Cheese - microbiology Dairy products DNA, Bacterial - analysis Food industries Food microbiology Fundamental and applied biological sciences. Psychology Health hazards Infertility Milk and cheese industries. Ice creams Polymerase Chain Reaction - methods semi-nested PCR Sensitivity and Specificity soft cheeses |
| title | Detection of Brucella spp. in soft cheese by semi-nested polymerase chain reaction |
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