Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells: implications of low-responsiveness to bacterial components in the epidermal Langerhans cells
In the skin, there are unique dendritic cells called Langerhans cells, however, it remains unclear why this particular type of dendritic cell resides in the epidermis. Langerhans cell-like dendritic cells (LCs) can be generated from CD14 + monocytes in the presence of GM-CSF, IL-4, and TGF-β1. We co...
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| Veröffentlicht in: | Biochemical and biophysical research communications 2003-07, Vol.306 (3), p.674-679 |
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| container_title | Biochemical and biophysical research communications |
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| creator | Takeuchi, Junko Watari, Eiji Shinya, Eiji Norose, Yoshihiko Matsumoto, Misako Seya, Tsukasa Sugita, Masahiko Kawana, Seiji Takahashi, Hidemi |
| description | In the skin, there are unique dendritic cells called Langerhans cells, however, it remains unclear why this particular type of dendritic cell resides in the epidermis. Langerhans cell-like dendritic cells (LCs) can be generated from CD14
+ monocytes in the presence of GM-CSF, IL-4, and TGF-β1. We compared LCs with monocyte-derived dendritic cells (DCs) generated from CD14
+ monocytes in the presence of GM-CSF and IL-4 and examined the effect of exposure to two distinct bacterial stimuli via Toll-like receptors (TLRs), such as peptidoglycan (PGN) and lipopolysaccharide (LPS) on LCs and DCs. Although stimulation with both ligands induced a marked up-regulation of CD83 expression on DCs, PGN but not LPS elicited up-regulation of expression CD83 on LCs. Consistent with these results, TLR2 and TLR4 were expressed on DCs, whereas only TLR2 was weakly detected on LCs. These findings suggest the actual feature of epidermal Langerhans cells with low-responsiveness to skin commensals. |
| doi_str_mv | 10.1016/S0006-291X(03)01022-2 |
| format | Article |
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+ monocytes in the presence of GM-CSF and IL-4 and examined the effect of exposure to two distinct bacterial stimuli via Toll-like receptors (TLRs), such as peptidoglycan (PGN) and lipopolysaccharide (LPS) on LCs and DCs. Although stimulation with both ligands induced a marked up-regulation of CD83 expression on DCs, PGN but not LPS elicited up-regulation of expression CD83 on LCs. Consistent with these results, TLR2 and TLR4 were expressed on DCs, whereas only TLR2 was weakly detected on LCs. These findings suggest the actual feature of epidermal Langerhans cells with low-responsiveness to skin commensals.</description><identifier>ISSN: 0006-291X</identifier><identifier>EISSN: 1090-2104</identifier><identifier>DOI: 10.1016/S0006-291X(03)01022-2</identifier><identifier>PMID: 12810071</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Bacteria ; Bacteria - metabolism ; Cell Size ; Cells, Cultured ; Cytoplasmic Granules - metabolism ; Dendritic cells ; Down-Regulation ; Epidermal Cells ; Epidermis - immunology ; Epidermis - metabolism ; Humans ; Interleukin-10 - metabolism ; Interleukin-12 - metabolism ; Langerhans cells ; Langerhans Cells - cytology ; Langerhans Cells - drug effects ; Langerhans Cells - immunology ; Langerhans Cells - metabolism ; Lipopolysaccharide ; Lipopolysaccharide Receptors - metabolism ; Lipopolysaccharides - pharmacology ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Monocytes - cytology ; Monocytes - drug effects ; Monocytes - immunology ; Monocytes - metabolism ; Peptidoglycan ; Peptidoglycan - pharmacology ; Phenotype ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Toll-like receptor ; Toll-Like Receptor 2 ; Toll-Like Receptor 4 ; Toll-Like Receptors ; Transforming growth factor β1</subject><ispartof>Biochemical and biophysical research communications, 2003-07, Vol.306 (3), p.674-679</ispartof><rights>2003 Elsevier Science (USA)</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c458t-3a0db8b95549744035f7ba0f8b01dfc03eb9a784c1b9a6097004630da3be94513</citedby><cites>FETCH-LOGICAL-c458t-3a0db8b95549744035f7ba0f8b01dfc03eb9a784c1b9a6097004630da3be94513</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0006-291X(03)01022-2$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27928,27929,45999</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12810071$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takeuchi, Junko</creatorcontrib><creatorcontrib>Watari, Eiji</creatorcontrib><creatorcontrib>Shinya, Eiji</creatorcontrib><creatorcontrib>Norose, Yoshihiko</creatorcontrib><creatorcontrib>Matsumoto, Misako</creatorcontrib><creatorcontrib>Seya, Tsukasa</creatorcontrib><creatorcontrib>Sugita, Masahiko</creatorcontrib><creatorcontrib>Kawana, Seiji</creatorcontrib><creatorcontrib>Takahashi, Hidemi</creatorcontrib><title>Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells: implications of low-responsiveness to bacterial components in the epidermal Langerhans cells</title><title>Biochemical and biophysical research communications</title><addtitle>Biochem Biophys Res Commun</addtitle><description>In the skin, there are unique dendritic cells called Langerhans cells, however, it remains unclear why this particular type of dendritic cell resides in the epidermis. Langerhans cell-like dendritic cells (LCs) can be generated from CD14
+ monocytes in the presence of GM-CSF, IL-4, and TGF-β1. We compared LCs with monocyte-derived dendritic cells (DCs) generated from CD14
+ monocytes in the presence of GM-CSF and IL-4 and examined the effect of exposure to two distinct bacterial stimuli via Toll-like receptors (TLRs), such as peptidoglycan (PGN) and lipopolysaccharide (LPS) on LCs and DCs. Although stimulation with both ligands induced a marked up-regulation of CD83 expression on DCs, PGN but not LPS elicited up-regulation of expression CD83 on LCs. Consistent with these results, TLR2 and TLR4 were expressed on DCs, whereas only TLR2 was weakly detected on LCs. These findings suggest the actual feature of epidermal Langerhans cells with low-responsiveness to skin commensals.</description><subject>Bacteria</subject><subject>Bacteria - metabolism</subject><subject>Cell Size</subject><subject>Cells, Cultured</subject><subject>Cytoplasmic Granules - metabolism</subject><subject>Dendritic cells</subject><subject>Down-Regulation</subject><subject>Epidermal Cells</subject><subject>Epidermis - immunology</subject><subject>Epidermis - metabolism</subject><subject>Humans</subject><subject>Interleukin-10 - metabolism</subject><subject>Interleukin-12 - metabolism</subject><subject>Langerhans cells</subject><subject>Langerhans Cells - cytology</subject><subject>Langerhans Cells - drug effects</subject><subject>Langerhans Cells - immunology</subject><subject>Langerhans Cells - metabolism</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharide Receptors - metabolism</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Monocytes - cytology</subject><subject>Monocytes - drug effects</subject><subject>Monocytes - immunology</subject><subject>Monocytes - metabolism</subject><subject>Peptidoglycan</subject><subject>Peptidoglycan - pharmacology</subject><subject>Phenotype</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Toll-like receptor</subject><subject>Toll-Like Receptor 2</subject><subject>Toll-Like Receptor 4</subject><subject>Toll-Like Receptors</subject><subject>Transforming growth factor β1</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS1ERaeFRwB5hejC9Dp2_tggVP4qjcSCIrGzHOemNSR2anta-nC8G87MAFI3XV1b9zvnWD6EPOfwmgOvTr8CQMWKln9_BeIEOBQFKx6RFYcWWMFBPiarf8ghOYrxBwDnsmqfkENeNByg5ivy-72_dSzg5WbUyXpH_UAv_Diy0f5EGtDgnHyg-GsOGOMCWEcn77y5S8h6DPYGe7rW7hLDlXaRGvwrXk7xDbXTPFqzNY-L--hvc16c8zVrXXalydNOm5TN9EiNn_IOXYpLVLpCirPNQVPe3cuJT8nBoMeIz_bzmHz7-OHi7DNbf_l0fvZuzYwsm8SEhr5rurYsZVtLCaIc6k7D0HTA-8GAwK7VdSMNz7OCtgaQlYBeiw5bWXJxTF7ufOfgrzcYk5psXF6gHfpNVLUQdVk28kGwAC4bIZoMljvQBB9jwEHNwU463CkOailYbQtWS3sKhNoWrIqse7EP2HQT9v9V-0Yz8HYHYP6PG4tBRWPRGextbjOp3tsHIv4AbG-6cQ</recordid><startdate>20030704</startdate><enddate>20030704</enddate><creator>Takeuchi, Junko</creator><creator>Watari, Eiji</creator><creator>Shinya, Eiji</creator><creator>Norose, Yoshihiko</creator><creator>Matsumoto, Misako</creator><creator>Seya, Tsukasa</creator><creator>Sugita, Masahiko</creator><creator>Kawana, Seiji</creator><creator>Takahashi, Hidemi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030704</creationdate><title>Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells: implications of low-responsiveness to bacterial components in the epidermal Langerhans cells</title><author>Takeuchi, Junko ; Watari, Eiji ; Shinya, Eiji ; Norose, Yoshihiko ; Matsumoto, Misako ; Seya, Tsukasa ; Sugita, Masahiko ; Kawana, Seiji ; Takahashi, Hidemi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c458t-3a0db8b95549744035f7ba0f8b01dfc03eb9a784c1b9a6097004630da3be94513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Bacteria</topic><topic>Bacteria - metabolism</topic><topic>Cell Size</topic><topic>Cells, Cultured</topic><topic>Cytoplasmic Granules - metabolism</topic><topic>Dendritic cells</topic><topic>Down-Regulation</topic><topic>Epidermal Cells</topic><topic>Epidermis - immunology</topic><topic>Epidermis - metabolism</topic><topic>Humans</topic><topic>Interleukin-10 - metabolism</topic><topic>Interleukin-12 - metabolism</topic><topic>Langerhans cells</topic><topic>Langerhans Cells - cytology</topic><topic>Langerhans Cells - drug effects</topic><topic>Langerhans Cells - immunology</topic><topic>Langerhans Cells - metabolism</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharide Receptors - metabolism</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Membrane Glycoproteins - genetics</topic><topic>Membrane Glycoproteins - metabolism</topic><topic>Monocytes - cytology</topic><topic>Monocytes - drug effects</topic><topic>Monocytes - immunology</topic><topic>Monocytes - metabolism</topic><topic>Peptidoglycan</topic><topic>Peptidoglycan - pharmacology</topic><topic>Phenotype</topic><topic>Receptors, Cell Surface - genetics</topic><topic>Receptors, Cell Surface - metabolism</topic><topic>Toll-like receptor</topic><topic>Toll-Like Receptor 2</topic><topic>Toll-Like Receptor 4</topic><topic>Toll-Like Receptors</topic><topic>Transforming growth factor β1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takeuchi, Junko</creatorcontrib><creatorcontrib>Watari, Eiji</creatorcontrib><creatorcontrib>Shinya, Eiji</creatorcontrib><creatorcontrib>Norose, Yoshihiko</creatorcontrib><creatorcontrib>Matsumoto, Misako</creatorcontrib><creatorcontrib>Seya, Tsukasa</creatorcontrib><creatorcontrib>Sugita, Masahiko</creatorcontrib><creatorcontrib>Kawana, Seiji</creatorcontrib><creatorcontrib>Takahashi, Hidemi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biochemical and biophysical research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takeuchi, Junko</au><au>Watari, Eiji</au><au>Shinya, Eiji</au><au>Norose, Yoshihiko</au><au>Matsumoto, Misako</au><au>Seya, Tsukasa</au><au>Sugita, Masahiko</au><au>Kawana, Seiji</au><au>Takahashi, Hidemi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells: implications of low-responsiveness to bacterial components in the epidermal Langerhans cells</atitle><jtitle>Biochemical and biophysical research communications</jtitle><addtitle>Biochem Biophys Res Commun</addtitle><date>2003-07-04</date><risdate>2003</risdate><volume>306</volume><issue>3</issue><spage>674</spage><epage>679</epage><pages>674-679</pages><issn>0006-291X</issn><eissn>1090-2104</eissn><abstract>In the skin, there are unique dendritic cells called Langerhans cells, however, it remains unclear why this particular type of dendritic cell resides in the epidermis. Langerhans cell-like dendritic cells (LCs) can be generated from CD14
+ monocytes in the presence of GM-CSF, IL-4, and TGF-β1. We compared LCs with monocyte-derived dendritic cells (DCs) generated from CD14
+ monocytes in the presence of GM-CSF and IL-4 and examined the effect of exposure to two distinct bacterial stimuli via Toll-like receptors (TLRs), such as peptidoglycan (PGN) and lipopolysaccharide (LPS) on LCs and DCs. Although stimulation with both ligands induced a marked up-regulation of CD83 expression on DCs, PGN but not LPS elicited up-regulation of expression CD83 on LCs. Consistent with these results, TLR2 and TLR4 were expressed on DCs, whereas only TLR2 was weakly detected on LCs. These findings suggest the actual feature of epidermal Langerhans cells with low-responsiveness to skin commensals.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>12810071</pmid><doi>10.1016/S0006-291X(03)01022-2</doi><tpages>6</tpages></addata></record> |
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| subjects | Bacteria Bacteria - metabolism Cell Size Cells, Cultured Cytoplasmic Granules - metabolism Dendritic cells Down-Regulation Epidermal Cells Epidermis - immunology Epidermis - metabolism Humans Interleukin-10 - metabolism Interleukin-12 - metabolism Langerhans cells Langerhans Cells - cytology Langerhans Cells - drug effects Langerhans Cells - immunology Langerhans Cells - metabolism Lipopolysaccharide Lipopolysaccharide Receptors - metabolism Lipopolysaccharides - pharmacology Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Monocytes - cytology Monocytes - drug effects Monocytes - immunology Monocytes - metabolism Peptidoglycan Peptidoglycan - pharmacology Phenotype Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Toll-like receptor Toll-Like Receptor 2 Toll-Like Receptor 4 Toll-Like Receptors Transforming growth factor β1 |
| title | Down-regulation of Toll-like receptor expression in monocyte-derived Langerhans cell-like cells: implications of low-responsiveness to bacterial components in the epidermal Langerhans cells |
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