Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil
ABSTRACT Determining the population density of the spinach wilt pathogen Fusarium oxysporum f. sp. spinaciae in soil with conventional Fusarium-selective media is quite difficult because nonpathogenic strains of F. oxysporum also grow on those media and are indistinguishable from the pathogen. There...
Gespeichert in:
| Veröffentlicht in: | Phytopathology 2003-09, Vol.93 (9), p.1173-1181 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1181 |
|---|---|
| container_issue | 9 |
| container_start_page | 1173 |
| container_title | Phytopathology |
| container_volume | 93 |
| creator | Takehara, T Kuniyasu, K Mori, M Hagiwara, H |
| description | ABSTRACT Determining the population density of the spinach wilt pathogen Fusarium oxysporum f. sp. spinaciae in soil with conventional Fusarium-selective media is quite difficult because nonpathogenic strains of F. oxysporum also grow on those media and are indistinguishable from the pathogen. Therefore, a nitrate-nonutilizing (nit) mutant of the pathogen and corresponding selective media were tested in an experimental approach to determine the population density of the pathogen. Colony forming units of the pathogen were countable after soil-dilution plating onto nit mutant-selective media MMCPA, CMP, and CGMBP. Colony forming units of wild-type Fusarium spp. were countable using a wildtype Fusarium-selective medium, GMBP. By combining nit mutant- and wild-type-selective media, the population densities of pathogenic and nonpathogenic F. oxysporum in the same soil could be measured selectively. This method was useful in studying population dynamics of the pathogen after different soil treatments. Soil disinfested with hot water or chloropicrin was amended with the nit mutant pathogen, and subsequent changes in population densities of the pathogen were compared with those in nontreated field soil. The pathogen rapidly proliferated in disinfested soil and wilt developed faster than in nontreated soil. When a nonpathogenic isolate of F. oxysporum was added at high density to sterilized soil prior to the pathogen, growth of the pathogen was greatly suppressed. Nonpathogenic F. oxysporum could not, however, reduce the density of preexisting pathogen. |
| doi_str_mv | 10.1094/phyto.2003.93.9.1173 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_733747283</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>733747283</sourcerecordid><originalsourceid>FETCH-LOGICAL-c528t-92de2ce02db8c8a898398ae0832e00d1b10b2f3ecda35d918b6892cf492764583</originalsourceid><addsrcrecordid>eNp9kd9qFDEUh4Modq2-gWgQ1KsZ829mkksp1gqFCnZBr8LZTKamzCTTJCNdH8DnNsMuFLwQTsgh-c4HyQ-hl5TUlCjxYf65z6FmhPBalaop7fgjtKFK8KprpXiMNuWOVkqo7yfoWUq3hJBONu1TdEKlEoISvkF_tsniMGDA3uUI2VY--CW70f12_gZPSwafMfgeJztak90viyfbO8A5YHsPk_MWz2FeRsgueNzvfTkzaXWeLwmiWyYc7vdpDrF0Q43TvC7nwTiw2HmcghufoycDjMm-OO6naHv-6frsorq8-vzl7ONlZRomc6VYb5mxhPU7aSRIJbmSYInkzBLS0x0lOzZwa3rgTa-o3LVSMTMIxbpWNJKfovcH7xzD3WJT1pNLxo4jeBuWpDvOO9ExyQv57r8klVIq2rUFfPMPeBuW6MsrNOOUMVGMBRIHyMSQUrSDnqObIO41JXqNU3-9-HF9pdc4tSql1zjL2Kuje9mVX38YOuZXgLdHAJKBcYjgjUsPXENaKZuucK8P3ABBw00szPYbI5STYmFtQ_hfOeyzow</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>231224374</pqid></control><display><type>article</type><title>Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><source>American Phytopathological Society Journal Back Issues</source><creator>Takehara, T ; Kuniyasu, K ; Mori, M ; Hagiwara, H</creator><creatorcontrib>Takehara, T ; Kuniyasu, K ; Mori, M ; Hagiwara, H</creatorcontrib><description>ABSTRACT Determining the population density of the spinach wilt pathogen Fusarium oxysporum f. sp. spinaciae in soil with conventional Fusarium-selective media is quite difficult because nonpathogenic strains of F. oxysporum also grow on those media and are indistinguishable from the pathogen. Therefore, a nitrate-nonutilizing (nit) mutant of the pathogen and corresponding selective media were tested in an experimental approach to determine the population density of the pathogen. Colony forming units of the pathogen were countable after soil-dilution plating onto nit mutant-selective media MMCPA, CMP, and CGMBP. Colony forming units of wild-type Fusarium spp. were countable using a wildtype Fusarium-selective medium, GMBP. By combining nit mutant- and wild-type-selective media, the population densities of pathogenic and nonpathogenic F. oxysporum in the same soil could be measured selectively. This method was useful in studying population dynamics of the pathogen after different soil treatments. Soil disinfested with hot water or chloropicrin was amended with the nit mutant pathogen, and subsequent changes in population densities of the pathogen were compared with those in nontreated field soil. The pathogen rapidly proliferated in disinfested soil and wilt developed faster than in nontreated soil. When a nonpathogenic isolate of F. oxysporum was added at high density to sterilized soil prior to the pathogen, growth of the pathogen was greatly suppressed. Nonpathogenic F. oxysporum could not, however, reduce the density of preexisting pathogen.</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/phyto.2003.93.9.1173</identifier><identifier>PMID: 18944103</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>Biological and medical sciences ; Control ; Fundamental and applied biological sciences. Psychology ; Fungal plant pathogens ; Fusarium oxysporum ; hot water treatment ; inoculum density ; mutants ; nonpathogenic strains ; Phytopathology. Animal pests. Plant and forest protection ; plant pathogenic fungi ; population dynamics ; selective media ; soil fungi ; soil sterilization ; spinach ; spinach wilt ; Spinacia oleracea</subject><ispartof>Phytopathology, 2003-09, Vol.93 (9), p.1173-1181</ispartof><rights>2004 INIST-CNRS</rights><rights>Copyright American Phytopathological Society Sep 2003</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-92de2ce02db8c8a898398ae0832e00d1b10b2f3ecda35d918b6892cf492764583</citedby><cites>FETCH-LOGICAL-c528t-92de2ce02db8c8a898398ae0832e00d1b10b2f3ecda35d918b6892cf492764583</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3710,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15068857$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18944103$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takehara, T</creatorcontrib><creatorcontrib>Kuniyasu, K</creatorcontrib><creatorcontrib>Mori, M</creatorcontrib><creatorcontrib>Hagiwara, H</creatorcontrib><title>Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil</title><title>Phytopathology</title><addtitle>Phytopathology</addtitle><description>ABSTRACT Determining the population density of the spinach wilt pathogen Fusarium oxysporum f. sp. spinaciae in soil with conventional Fusarium-selective media is quite difficult because nonpathogenic strains of F. oxysporum also grow on those media and are indistinguishable from the pathogen. Therefore, a nitrate-nonutilizing (nit) mutant of the pathogen and corresponding selective media were tested in an experimental approach to determine the population density of the pathogen. Colony forming units of the pathogen were countable after soil-dilution plating onto nit mutant-selective media MMCPA, CMP, and CGMBP. Colony forming units of wild-type Fusarium spp. were countable using a wildtype Fusarium-selective medium, GMBP. By combining nit mutant- and wild-type-selective media, the population densities of pathogenic and nonpathogenic F. oxysporum in the same soil could be measured selectively. This method was useful in studying population dynamics of the pathogen after different soil treatments. Soil disinfested with hot water or chloropicrin was amended with the nit mutant pathogen, and subsequent changes in population densities of the pathogen were compared with those in nontreated field soil. The pathogen rapidly proliferated in disinfested soil and wilt developed faster than in nontreated soil. When a nonpathogenic isolate of F. oxysporum was added at high density to sterilized soil prior to the pathogen, growth of the pathogen was greatly suppressed. Nonpathogenic F. oxysporum could not, however, reduce the density of preexisting pathogen.</description><subject>Biological and medical sciences</subject><subject>Control</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungal plant pathogens</subject><subject>Fusarium oxysporum</subject><subject>hot water treatment</subject><subject>inoculum density</subject><subject>mutants</subject><subject>nonpathogenic strains</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>plant pathogenic fungi</subject><subject>population dynamics</subject><subject>selective media</subject><subject>soil fungi</subject><subject>soil sterilization</subject><subject>spinach</subject><subject>spinach wilt</subject><subject>Spinacia oleracea</subject><issn>0031-949X</issn><issn>1943-7684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kd9qFDEUh4Modq2-gWgQ1KsZ829mkksp1gqFCnZBr8LZTKamzCTTJCNdH8DnNsMuFLwQTsgh-c4HyQ-hl5TUlCjxYf65z6FmhPBalaop7fgjtKFK8KprpXiMNuWOVkqo7yfoWUq3hJBONu1TdEKlEoISvkF_tsniMGDA3uUI2VY--CW70f12_gZPSwafMfgeJztak90viyfbO8A5YHsPk_MWz2FeRsgueNzvfTkzaXWeLwmiWyYc7vdpDrF0Q43TvC7nwTiw2HmcghufoycDjMm-OO6naHv-6frsorq8-vzl7ONlZRomc6VYb5mxhPU7aSRIJbmSYInkzBLS0x0lOzZwa3rgTa-o3LVSMTMIxbpWNJKfovcH7xzD3WJT1pNLxo4jeBuWpDvOO9ExyQv57r8klVIq2rUFfPMPeBuW6MsrNOOUMVGMBRIHyMSQUrSDnqObIO41JXqNU3-9-HF9pdc4tSql1zjL2Kuje9mVX38YOuZXgLdHAJKBcYjgjUsPXENaKZuucK8P3ABBw00szPYbI5STYmFtQ_hfOeyzow</recordid><startdate>20030901</startdate><enddate>20030901</enddate><creator>Takehara, T</creator><creator>Kuniyasu, K</creator><creator>Mori, M</creator><creator>Hagiwara, H</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PCBAR</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>S0X</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030901</creationdate><title>Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil</title><author>Takehara, T ; Kuniyasu, K ; Mori, M ; Hagiwara, H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c528t-92de2ce02db8c8a898398ae0832e00d1b10b2f3ecda35d918b6892cf492764583</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Biological and medical sciences</topic><topic>Control</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungal plant pathogens</topic><topic>Fusarium oxysporum</topic><topic>hot water treatment</topic><topic>inoculum density</topic><topic>mutants</topic><topic>nonpathogenic strains</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>plant pathogenic fungi</topic><topic>population dynamics</topic><topic>selective media</topic><topic>soil fungi</topic><topic>soil sterilization</topic><topic>spinach</topic><topic>spinach wilt</topic><topic>Spinacia oleracea</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takehara, T</creatorcontrib><creatorcontrib>Kuniyasu, K</creatorcontrib><creatorcontrib>Mori, M</creatorcontrib><creatorcontrib>Hagiwara, H</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>SIRS Editorial</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takehara, T</au><au>Kuniyasu, K</au><au>Mori, M</au><au>Hagiwara, H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil</atitle><jtitle>Phytopathology</jtitle><addtitle>Phytopathology</addtitle><date>2003-09-01</date><risdate>2003</risdate><volume>93</volume><issue>9</issue><spage>1173</spage><epage>1181</epage><pages>1173-1181</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><coden>PHYTAJ</coden><abstract>ABSTRACT Determining the population density of the spinach wilt pathogen Fusarium oxysporum f. sp. spinaciae in soil with conventional Fusarium-selective media is quite difficult because nonpathogenic strains of F. oxysporum also grow on those media and are indistinguishable from the pathogen. Therefore, a nitrate-nonutilizing (nit) mutant of the pathogen and corresponding selective media were tested in an experimental approach to determine the population density of the pathogen. Colony forming units of the pathogen were countable after soil-dilution plating onto nit mutant-selective media MMCPA, CMP, and CGMBP. Colony forming units of wild-type Fusarium spp. were countable using a wildtype Fusarium-selective medium, GMBP. By combining nit mutant- and wild-type-selective media, the population densities of pathogenic and nonpathogenic F. oxysporum in the same soil could be measured selectively. This method was useful in studying population dynamics of the pathogen after different soil treatments. Soil disinfested with hot water or chloropicrin was amended with the nit mutant pathogen, and subsequent changes in population densities of the pathogen were compared with those in nontreated field soil. The pathogen rapidly proliferated in disinfested soil and wilt developed faster than in nontreated soil. When a nonpathogenic isolate of F. oxysporum was added at high density to sterilized soil prior to the pathogen, growth of the pathogen was greatly suppressed. Nonpathogenic F. oxysporum could not, however, reduce the density of preexisting pathogen.</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><pmid>18944103</pmid><doi>10.1094/phyto.2003.93.9.1173</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0031-949X |
| ispartof | Phytopathology, 2003-09, Vol.93 (9), p.1173-1181 |
| issn | 0031-949X 1943-7684 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_733747283 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues |
| subjects | Biological and medical sciences Control Fundamental and applied biological sciences. Psychology Fungal plant pathogens Fusarium oxysporum hot water treatment inoculum density mutants nonpathogenic strains Phytopathology. Animal pests. Plant and forest protection plant pathogenic fungi population dynamics selective media soil fungi soil sterilization spinach spinach wilt Spinacia oleracea |
| title | Use of a nitrate-nonutilizing mutant and selective media to examine population dynamics of Fusarium oxysporum f. sp. spinaciae in soil |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T02%3A54%3A43IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Use%20of%20a%20nitrate-nonutilizing%20mutant%20and%20selective%20media%20to%20examine%20population%20dynamics%20of%20Fusarium%20oxysporum%20f.%20sp.%20spinaciae%20in%20soil&rft.jtitle=Phytopathology&rft.au=Takehara,%20T&rft.date=2003-09-01&rft.volume=93&rft.issue=9&rft.spage=1173&rft.epage=1181&rft.pages=1173-1181&rft.issn=0031-949X&rft.eissn=1943-7684&rft.coden=PHYTAJ&rft_id=info:doi/10.1094/phyto.2003.93.9.1173&rft_dat=%3Cproquest_cross%3E733747283%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=231224374&rft_id=info:pmid/18944103&rfr_iscdi=true |