Hypovirulence and Double-Stranded RNA in Botrytis cinerea
Twenty-one strains of Botrytis cinerea isolated from 13 species of plants grown in China were compared for pathogenicity on Brassica napus, mycelial growth on potato dextrose agar, and presence of double-stranded (ds)RNA. The results showed that the strain CanBc-1 was severely debilitated in pathoge...
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| Veröffentlicht in: | Phytopathology 2007-12, Vol.97 (12), p.1590-1599 |
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| creator | Wu, M.D Zhang, L Li, G.Q Jiang, H Hou, M.S Huang, H.C |
| description | Twenty-one strains of Botrytis cinerea isolated from 13 species of plants grown in China were compared for pathogenicity on Brassica napus, mycelial growth on potato dextrose agar, and presence of double-stranded (ds)RNA. The results showed that the strain CanBc-1 was severely debilitated in pathogenicity and mycelial growth, compared with the 20 virulent strains. A dsRNA of approximately 3.0 kb in length was detected in CanBc-1 and 4 hypovirulent single-conidium (SC) isolates of CanBc-1, but was not detected in the 20 virulent strains of B. cinerea and 4 virulent SC isolates of CanBc-1. Results of the horizontal transmission experiment showed that the hypovirulent trait of CanBc-1 was transmissible and the 3.0-kb dsRNA was involved in the transmission of hypovirulence. Analysis of a 920-bp cDNA sequence generated from the 3.0-kb dsRNA of CanBc-1 indicated that the dsRNA element was a mycovirus, designated as B. cinerea debilitation-related virus (BcDRV). Further analyses showed that BcDRV is closely related to Ophiostoma mitovirus 3b infecting O. novo-ulmi, the causal agent of Dutch elm disease. Mitochondria and cytoplasm in hyphal cells of CanBc-1 became degenerated, compared with the virulent isolate CanBc-1c-66 of B cinerea. This is the first report on the occurrence of Mitovirus-associated hypovirulence in B. cinerea. |
| doi_str_mv | 10.1094/phyto-97-12-1590 |
| format | Article |
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The results showed that the strain CanBc-1 was severely debilitated in pathogenicity and mycelial growth, compared with the 20 virulent strains. A dsRNA of approximately 3.0 kb in length was detected in CanBc-1 and 4 hypovirulent single-conidium (SC) isolates of CanBc-1, but was not detected in the 20 virulent strains of B. cinerea and 4 virulent SC isolates of CanBc-1. Results of the horizontal transmission experiment showed that the hypovirulent trait of CanBc-1 was transmissible and the 3.0-kb dsRNA was involved in the transmission of hypovirulence. Analysis of a 920-bp cDNA sequence generated from the 3.0-kb dsRNA of CanBc-1 indicated that the dsRNA element was a mycovirus, designated as B. cinerea debilitation-related virus (BcDRV). Further analyses showed that BcDRV is closely related to Ophiostoma mitovirus 3b infecting O. novo-ulmi, the causal agent of Dutch elm disease. Mitochondria and cytoplasm in hyphal cells of CanBc-1 became degenerated, compared with the virulent isolate CanBc-1c-66 of B cinerea. This is the first report on the occurrence of Mitovirus-associated hypovirulence in B. cinerea.</description><identifier>ISSN: 0031-949X</identifier><identifier>EISSN: 1943-7684</identifier><identifier>DOI: 10.1094/phyto-97-12-1590</identifier><identifier>PMID: 18943720</identifier><identifier>CODEN: PHYTAJ</identifier><language>eng</language><publisher>St. Paul, MN: American Phytopathological Society</publisher><subject>Biological and medical sciences ; Botrytis cinerea ; Brassica napus ; double-stranded RNA ; Fundamental and applied biological sciences. Psychology ; fungal diseases of plants ; Fungal plant pathogens ; gene transfer ; host plants ; host range ; Mitovirus ; mycelium ; mycoviruses ; Ophiostoma ; pathogenicity ; Phytopathology. Animal pests. Plant and forest protection ; sexual reproduction ; Solanum tuberosum ; strain differences ; strains ; virulence</subject><ispartof>Phytopathology, 2007-12, Vol.97 (12), p.1590-1599</ispartof><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c538t-8700bafe4d419d8375629843cca532428afc3aff9e7e35db7ed1f1af4c6c78a73</citedby><cites>FETCH-LOGICAL-c538t-8700bafe4d419d8375629843cca532428afc3aff9e7e35db7ed1f1af4c6c78a73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,782,786,3728,27933,27934</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=19880998$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18943720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, M.D</creatorcontrib><creatorcontrib>Zhang, L</creatorcontrib><creatorcontrib>Li, G.Q</creatorcontrib><creatorcontrib>Jiang, H</creatorcontrib><creatorcontrib>Hou, M.S</creatorcontrib><creatorcontrib>Huang, H.C</creatorcontrib><title>Hypovirulence and Double-Stranded RNA in Botrytis cinerea</title><title>Phytopathology</title><addtitle>Phytopathology</addtitle><description>Twenty-one strains of Botrytis cinerea isolated from 13 species of plants grown in China were compared for pathogenicity on Brassica napus, mycelial growth on potato dextrose agar, and presence of double-stranded (ds)RNA. The results showed that the strain CanBc-1 was severely debilitated in pathogenicity and mycelial growth, compared with the 20 virulent strains. A dsRNA of approximately 3.0 kb in length was detected in CanBc-1 and 4 hypovirulent single-conidium (SC) isolates of CanBc-1, but was not detected in the 20 virulent strains of B. cinerea and 4 virulent SC isolates of CanBc-1. Results of the horizontal transmission experiment showed that the hypovirulent trait of CanBc-1 was transmissible and the 3.0-kb dsRNA was involved in the transmission of hypovirulence. Analysis of a 920-bp cDNA sequence generated from the 3.0-kb dsRNA of CanBc-1 indicated that the dsRNA element was a mycovirus, designated as B. cinerea debilitation-related virus (BcDRV). Further analyses showed that BcDRV is closely related to Ophiostoma mitovirus 3b infecting O. novo-ulmi, the causal agent of Dutch elm disease. Mitochondria and cytoplasm in hyphal cells of CanBc-1 became degenerated, compared with the virulent isolate CanBc-1c-66 of B cinerea. This is the first report on the occurrence of Mitovirus-associated hypovirulence in B. cinerea.</description><subject>Biological and medical sciences</subject><subject>Botrytis cinerea</subject><subject>Brassica napus</subject><subject>double-stranded RNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>fungal diseases of plants</subject><subject>Fungal plant pathogens</subject><subject>gene transfer</subject><subject>host plants</subject><subject>host range</subject><subject>Mitovirus</subject><subject>mycelium</subject><subject>mycoviruses</subject><subject>Ophiostoma</subject><subject>pathogenicity</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>sexual reproduction</subject><subject>Solanum tuberosum</subject><subject>strain differences</subject><subject>strains</subject><subject>virulence</subject><issn>0031-949X</issn><issn>1943-7684</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp90EtLxDAUBeAgio6PvSvtRl1Fb5q0N1n6HkFUfICuQiZNtNJpx6QV5t8bmQF3ri4XvnMWh5BdBscMlDiZfcz7jiqkLKesULBCRkwJTrGUYpWMADijSqjXDbIZ4ycAoCzKdbLBZFKYw4io8XzWfddhaFxrXWbaKrvohknj6FMf0ueq7PHuNKvb7Kzrw7yvY2br1gVntsmaN010O8u7RV6uLp_Px_T2_vrm_PSW2oLLnkoEmBjvRCWYqiTHosyVFNxaU_Bc5NJ4y433yqHjRTVBVzHPjBe2tCgN8i1ytOidhe5rcLHX0zpa1zSmdd0QNXKOopRYJHn4r2SqRISyTBAW0IYuxuC8noV6asJcM9C_w-qH8dvzvVaoWa5_h02RvWX3MJm66i-wXDKBgyUw0ZrGp_FsHf-ckhKUksntL5w3nTbvIZmXpxwYB5CFQJT8B0SKigs</recordid><startdate>20071201</startdate><enddate>20071201</enddate><creator>Wu, M.D</creator><creator>Zhang, L</creator><creator>Li, G.Q</creator><creator>Jiang, H</creator><creator>Hou, M.S</creator><creator>Huang, H.C</creator><general>American Phytopathological Society</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20071201</creationdate><title>Hypovirulence and Double-Stranded RNA in Botrytis cinerea</title><author>Wu, M.D ; Zhang, L ; Li, G.Q ; Jiang, H ; Hou, M.S ; Huang, H.C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c538t-8700bafe4d419d8375629843cca532428afc3aff9e7e35db7ed1f1af4c6c78a73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Biological and medical sciences</topic><topic>Botrytis cinerea</topic><topic>Brassica napus</topic><topic>double-stranded RNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>fungal diseases of plants</topic><topic>Fungal plant pathogens</topic><topic>gene transfer</topic><topic>host plants</topic><topic>host range</topic><topic>Mitovirus</topic><topic>mycelium</topic><topic>mycoviruses</topic><topic>Ophiostoma</topic><topic>pathogenicity</topic><topic>Phytopathology. Animal pests. Plant and forest protection</topic><topic>sexual reproduction</topic><topic>Solanum tuberosum</topic><topic>strain differences</topic><topic>strains</topic><topic>virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, M.D</creatorcontrib><creatorcontrib>Zhang, L</creatorcontrib><creatorcontrib>Li, G.Q</creatorcontrib><creatorcontrib>Jiang, H</creatorcontrib><creatorcontrib>Hou, M.S</creatorcontrib><creatorcontrib>Huang, H.C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Phytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, M.D</au><au>Zhang, L</au><au>Li, G.Q</au><au>Jiang, H</au><au>Hou, M.S</au><au>Huang, H.C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hypovirulence and Double-Stranded RNA in Botrytis cinerea</atitle><jtitle>Phytopathology</jtitle><addtitle>Phytopathology</addtitle><date>2007-12-01</date><risdate>2007</risdate><volume>97</volume><issue>12</issue><spage>1590</spage><epage>1599</epage><pages>1590-1599</pages><issn>0031-949X</issn><eissn>1943-7684</eissn><coden>PHYTAJ</coden><abstract>Twenty-one strains of Botrytis cinerea isolated from 13 species of plants grown in China were compared for pathogenicity on Brassica napus, mycelial growth on potato dextrose agar, and presence of double-stranded (ds)RNA. The results showed that the strain CanBc-1 was severely debilitated in pathogenicity and mycelial growth, compared with the 20 virulent strains. A dsRNA of approximately 3.0 kb in length was detected in CanBc-1 and 4 hypovirulent single-conidium (SC) isolates of CanBc-1, but was not detected in the 20 virulent strains of B. cinerea and 4 virulent SC isolates of CanBc-1. Results of the horizontal transmission experiment showed that the hypovirulent trait of CanBc-1 was transmissible and the 3.0-kb dsRNA was involved in the transmission of hypovirulence. Analysis of a 920-bp cDNA sequence generated from the 3.0-kb dsRNA of CanBc-1 indicated that the dsRNA element was a mycovirus, designated as B. cinerea debilitation-related virus (BcDRV). Further analyses showed that BcDRV is closely related to Ophiostoma mitovirus 3b infecting O. novo-ulmi, the causal agent of Dutch elm disease. Mitochondria and cytoplasm in hyphal cells of CanBc-1 became degenerated, compared with the virulent isolate CanBc-1c-66 of B cinerea. This is the first report on the occurrence of Mitovirus-associated hypovirulence in B. cinerea.</abstract><cop>St. Paul, MN</cop><pub>American Phytopathological Society</pub><pmid>18943720</pmid><doi>10.1094/phyto-97-12-1590</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Biological and medical sciences Botrytis cinerea Brassica napus double-stranded RNA Fundamental and applied biological sciences. Psychology fungal diseases of plants Fungal plant pathogens gene transfer host plants host range Mitovirus mycelium mycoviruses Ophiostoma pathogenicity Phytopathology. Animal pests. Plant and forest protection sexual reproduction Solanum tuberosum strain differences strains virulence |
| title | Hypovirulence and Double-Stranded RNA in Botrytis cinerea |
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