Detection of the thermostable direct hemolysin gene ( tdh) and the thermostable direct hemolysin-related hemolysin gene ( trh) of Vibrio parahaemolyticus by polymerase chain reaction

Polymerase chain reaction (PCR) protocols were established for specific detection of the tdh and trh genes, the virulence marker genes of Vibrio parahaemolyticus encoding two related hemolysins. The tdh and trh genes are known to have sequence divergence of up to 3 · 3% and 16%, respectively. Attemp...

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Veröffentlicht in:	Molecular and cellular probes 1992-12, Vol.6 (6), p.477-487
Hauptverfasser:	Tada, Jun, Ohashi, Tetsuo, Nishimura, Naoyuki, Shirasaki, Yoshinari, Ozaki, Hiroko, Fukushima, Shigeru, Takano, Jun, Nishibuchi, Mitsuaki, Takeda, Yoshifumi
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Biomarkers DNA, Bacterial - genetics Feces - microbiology Fundamental and applied biological sciences. Psychology Genes, Bacterial Hemolysin Proteins - genetics Humans Microbiology Molecular Sequence Data Oligonucleotide Probes PCR Polymerase Chain Reaction Sensitivity and Specificity Techniques used in virology thermostable direct hemolysin gene Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification Vibrio parahaemolyticus - pathogenicity Virology Virulence
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